LOCUS       sequence-208264-        1173 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of proISG15.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Swatek KN, Aumayr M, Pruneda JN, Visser LJ, Berryman S, Kueck AF, 
            Geurink PP, Ovaa H, van Kuppeveld FJM, Tuthill TJ, Skern T, Komander
            D
  TITLE     Irreversible inactivation of ISG15 by a viral leader protease 
            enables alternative infection detection strategies.
  JOURNAL   Proc Natl Acad Sci U S A. 2018 Mar 6;115(10):2371-2376. doi: 
            10.1073/pnas.1710617115. Epub 2018 Feb 20.
  PUBMED    29463763
REFERENCE   2  (bases 1 to 1173)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1710617115"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2018-03-6- 6"; volume: "115"; issue: "10"; pages: "2371-2376"
COMMENT     Sequence Label: proISG15 (2-165)
FEATURES             Location/Qualifiers
     source          1..1173
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             44..61
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             71..94
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             610..627
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(680..698)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      694..741
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      778..1152
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(865..884)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 attttgttta actttaagaa ggagatatac catgggcagc agccatcatc atcatcatca
       61 cagcagcggt ctggaagttc tgtttcaggg cccgggctgg gacctgacgg tgaagatgct
      121 ggcgggcaac gaattccagg tgtccctgag cagctccatg tcggtgtcag agctgaaggc
      181 gcagatcacc cagaagatcg gcgtgcacgc cttccagcag cgtctggctg tccacccgag
      241 cggtgtggcg ctgcaggaca gggtccccct tgccagccag ggcctgggcc ccggcagcac
      301 ggtcctgctg gtggtggaca aatgcgacga acctctgagc atcctggtga ggaataacaa
      361 gggccgcagc agcacctacg aggtgcggct gacgcagacc gtggcccacc tgaagcagca
      421 agtgagcggg ctggagggtg tgcaggacga cctgttctgg ctgaccttcg aggggaagcc
      481 cctggaggac cagctcccgc tgggggagta cggcctcaag cccctgagca ccgtgttcat
      541 gaatctgcgc ctgcggggag gcggcacaga gcctggcggg cggagctaaa gctttctaga
      601 ccatttaaac accaccacca ccaccactga gatccggctg ctaacaaagc ccgaaaggaa
      661 gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa
      721 cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggattggc gaatgggacg
      781 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
      841 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
      901 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
      961 ctttacggca cctcgacccc aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     1021 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     1081 cttgttccaa ctggaaaaca ctcaccctat ctcggtctat cttttgattt aaagggattt
     1141 tgccgatttc ggcctatggt taaaaatgag ctg
//
