LOCUS       sequence-208297-        8413 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To test the effect of sequence on TDP43 splicing activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8413)
  TITLE     Rajat Rohatgi TDP43 plasmids
REFERENCE   2  (bases 1 to 8413)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHBS1201 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 6x-Phi]
FEATURES             Location/Qualifiers
     source          1..8413
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(271..290)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     540..558
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      2180..2189
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     enhancer        2342..2645
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2646..2849
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2800..2820
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      2911..2920
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2917..3618
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MGSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMAL
                     KLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARY
                     CDLPSKLGHKLN"
     misc_feature    3619..3684
                     /label=MCS
                     /note="multiple cloning site"
     CDS             3709..3765
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     protein_bind    complement(5011..5035)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     intron          5062..5634
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(5766..5785)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5831..5886
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5885..5904)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6284..6301)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6455..7043)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6535..6554)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7214..8005,8006..8074))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7837..7856
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8075..8179)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8247..8265
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 acagctcgtc catgccgccg gtggagtggc ggccctcggc gcgttcgtac tgttccacga
       61 tggtgtagtc ctcgttgtgg gaggtgatgt ccaacttgat gttgacgttg taggcgccgg
      121 gcagctgcac gggcttcttg gccttgtagg tggtcttgac ctcagcgtcg tagtggccgc
      181 cgtccttcag cttcagcctc tgcttgatct cgcccttcag ggcgccgtcc tcggggtaca
      241 tccgctcgga ggaggcctcc cagcccatgg tcttcttctg cattacgggg ccgtcggagg
      301 ggaagttggt gccgcgcagc ttcaccttgt agatgaactc gccgtcctgc agggaggagt
      361 cctgggtcac ggtcaccacg ccgccgtcct cgaagttcat cacgcgctcc cacttgaagc
      421 cctcggggaa ggacagcttc aagtagtcgg ggatgtcggc ggggtgcttc acgtaggcct
      481 tggagccgta catgaactga ggggacagga tgtcccaggc gaagggcagg gggccaccct
      541 tggtcacctt cagcttggcg gtctgggtgc cctcgtaggg gcggccctga ggaaaaaaaa
      601 ggaaagggca tgctcgccat gtgcaagata cagtgttgaa tgtggtgcaa aatataaaga
      661 tgtggaagaa aaagccactg aaaataatat gaggaaataa aaaactagag taaattatca
      721 gtggaacaat ttctattata caagacacta cacccataca ttctcctaat gctcatgtaa
      781 gaatttctcc aaaaatacct tccagcacta caaactagaa aaaaaaagag acatggacac
      841 caaattaagt tcttaatagt gaagaacaaa agaactacct tgcctgctcc agtggatcca
      901 gcaaccgcca acaactgtcc tctttctatc ttgaaattaa tatctttcag gacaggagta
      961 ccaagaagtg agaaattact gaagaagagg ctgtcatcac cattagaagt ttttctattg
     1021 ttattgtttt gttttgcttt ctcaaattat tccccaaatc cctgttaaaa aaaaacacac
     1081 acacacacac acacacacac atcaaaaata aaagatgagt ttgtcagata ttttcaatag
     1141 atgcttgttt tatgtaccat actcacctcg ccctcgccct cgatctcgaa ctcgtggccg
     1201 ttcacggagc cctccatgtg caccttgaag cgcatgaact ccttgatgat ggccatgtta
     1261 tcctcctcgc ccttgctggt tccggtgcct ccactttcat cttcaccttt agtgataccg
     1321 gctgccgtca cgaactcctt caggaccatg tgatcgcgct tctcgttcgg gtctttgctc
     1381 agcgtggtct gggtgctcag atagtggttg tccggcaggc gcaccgggcc gtcgccgatc
     1441 ggggtgttct gctgtttgtg gtcggcttcc tgctggctgc cgtcctcaac gttgtggcgg
     1501 atcttgaagt tagccttgat gccgttcttc tgcttgtcgg cttcgatacg gacgttgtgg
     1561 ctgttgtggt tgtactccag cttgtgaccc aggatgttgc cgtcctcctt gaagtcgttg
     1621 cccttcagct cgatgcggtt caccagggtg tcgccctcga acttcacctc cgcgcgggtc
     1681 ttgtaggtgc cgtcgtcctt gaactcgatg gtgcgctcct ggacgtagcc ttccggcatg
     1741 gcggacttga agaagtcgtg acgcttcatg tggtccgggt agcggctgaa gcactgcacg
     1801 ccgtaggtca gggtggtcac gagggtcggc cacggcaccg gcagcttgcc ggtggtgcag
     1861 atgaacttca gggtcagctt gccctcggtg gcatcgccct cgccctcgcc acgcacgctg
     1921 aacttgtggc cgtttacgtc gccgtccagc tcgactttga taggcacagt acctgtaaat
     1981 agttcctctc ctttactggt accatcatca gggaatgagc ttacatttct cctcttcagc
     2041 atctggtcat ccttcttagc tttcctcagc tccacattga cctctattct gcgacgcctc
     2101 atttctgtac tgtcttttcc cttgttcttg aatctgtgaa gacgggcagc tggtgtatta
     2161 gcattctcgt tggtggatcc catggtggca ccggtgaatt ctccaggcga tctgacggtt
     2221 cactaaacga gctctgctta tataggcctc ccaccgtaca cgccacctcg acatactcga
     2281 gtagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
     2341 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
     2401 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
     2461 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
     2521 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
     2581 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
     2641 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg
     2701 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
     2761 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
     2821 tacggtggga ggtctatata agcagagctg gtttagtgaa ccgtcagatc cgctagggat
     2881 cctctagtca gctgacgcgt taccaccggt gccaccatgg gaagcgagct gattaaggag
     2941 aacatgcaca tgaagctgta catggagggc accgtggaca accatcactt caagtgcaca
     3001 tccgagggcg aaggcaagcc ctacgagggc acccagacca tgagaatcaa ggtggtcgag
     3061 ggcggccctc tccccttcgc cttcgacatc ctggctacta gcttcctcta cggcagcaag
     3121 accttcatca accacaccca gggcatcccc gacttcttca agcagtcctt ccctgagggc
     3181 ttcacatggg agagagtcac cacatacgaa gacgggggcg tgctgaccgc tacccaggac
     3241 accagcctcc aggacggctg cctcatctac aacgtcaaga tcagaggggt gaacttcaca
     3301 tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg aggccttcac cgagacgctg
     3361 taccccgctg acggcggcct ggaaggcaga aacgacatgg ccctgaagct cgtgggcggg
     3421 agccatctga tcgcaaacgc caagaccaca tatagatcca agaaacccgc taagaacctc
     3481 aagatgcctg gcgtctacta tgtggactac agactggaaa gaatcaagga ggccaacaac
     3541 gagacctacg tcgagcagca cgaggtggca gtggccagat actgcgacct ccctagcaaa
     3601 ctggggcaca agcttaattc cggactcaga tctcgagctc aagcttcgaa ttctgcagtc
     3661 gacggtaccg cgggcccggg atccaccgga tctagaggag gaagcggagc tactaacttc
     3721 agcctgctga agcaggctgg agacgtggag gagaaccctg gacctatgtc tgaatatatt
     3781 cgggtaaccg aagatgagaa cgatgagccc attgaaatac catcggaaga cgatgggacg
     3841 gtgctgctct ccacggttac agcccagttt ccaggggcgt gtgggcttcg ctacaggaat
     3901 ccagtgtctc agtgtatgag aggtgtccgg ctggtagaag gaattctgca tgccccagat
     3961 gctggctggg gaaatctggt gtatgttgtc aactatccaa aagataacaa aagaaaaatg
     4021 gatgagacag atgcttcatc agcagtgaaa gtgaaaagag cagtccagaa aacatccgat
     4081 ttaatagtgt tgggtctccc atggaaaaca accgaacagg acctgaaaga gtattttagt
     4141 acctttggag aagttcttat ggtgcaggtc aagaaagatc ttaagactgg tcattcaaag
     4201 gggtttggct ttgttcgttt tacggaatat gaaacacaag tgaaagtaat gtcacagcga
     4261 catatgatag atggacgatg gtgtgactgc aaacttccta attctaagca aagccaagat
     4321 gagcctttga gaagcagaaa agtgtttgtg gggcgctgta cagaggacat gactgaggat
     4381 gagctgcggg agttcttctc tcagtacggg gatgtgatgg atgtcttcat ccccaagcca
     4441 ttcagggcct ttgcctttgt tacatttgca gatgatcaga ttgcgcagtc tctttgtgga
     4501 gaggacttga tcattaaagg aatcagcgtt catatatcca atgccgaacc taagcataac
     4561 agtaataggc agctagagcg ctccgggcgc ggcaatccgt ttggcggcgg gttcggaaac
     4621 cagaattcaa ggggtggcgg cgccggaggg ggatttggac tgggtaataa tcagggttct
     4681 aatatgggtg gcggtatgaa cgcgtttggg ttcagcatca accctgctat gatggcggct
     4741 gcacaggcgg ctctgcagtc atcatggggt atgatgggca tgctggcgtc acagcagaac
     4801 cagtccggcc cgagcggaaa caatcagaat caaggtaata tgcagcgtga gccaaaccag
     4861 gcgtttggat attccagtgg gaataactct ggaagtaata gcggcgcagc catcgggtgg
     4921 ggctctgcca gcaatgcggg atcagggagt aatggctttg gcggttttgg tgggtggggg
     4981 atgtcatcga tggatagtaa gtcaagctag acccagcttt cttgtacaaa gtggttgatc
     5041 ctctagactg agaacttcag ggtgagtttg gggacccttg attgttcttt ctttttcgct
     5101 attgtaaaat tcatgttata tggagggggc aaagttttca gggtgttgtt tagaatggga
     5161 agatgtccct tgtatcacca tggaccctca tgataatttt gtttctttca ctttctactc
     5221 tgttgacaac cattgtctcc tcttattttc ttttcatttt ctgtaacttt ttcgttaaac
     5281 tttagcttgc atttgtaacg aatttttaaa ttcacttttg tttatttgtc agattgtaag
     5341 tactttctct aatcactttt ttttcaaggc aatcagggta tattatattg tacttcagca
     5401 cagttttaga gaacaattgt tataattaaa tgataaggta gaatatttct gcatataaat
     5461 tctggctggc gtggaaatat tcttattggt agaaacaact acaccctggt catcatcctg
     5521 cctttctctt tatggttaca atgatataca ctgtttgaga tgaggataaa atactctgag
     5581 tccaaaccgg gcccctctgc taaccatgtt catgccttct tctctttcct acagctcctg
     5641 ggcaacgtgc tggttgttgt gctgtctcat cattttggca aagaattcac tcctcaggtg
     5701 caggctgcct atcagaaggt ggtggctggt gtggccaatg ccctggctca caaataccac
     5761 tgagatcttt ttccctctgc caaaaattat ggggacatca tgaagcccct tgagcatctg
     5821 acttctggct aataaaggaa atttattttc attgcaatag tgtgttggaa ttttttgtgt
     5881 ctctcactcg gaaggacata tgggagggca aatcatttaa aacatcagaa tgagtatttg
     5941 gtttagagtt tggcaacata tgcccatatg ctggctgcca tgaacaaagg ttggctataa
     6001 agaggtcatc agtatatgaa acagccccct gctgtccatt ccttattcca tagaaaagcc
     6061 ttgacttgag gttagatttt ttttatattt tgttttgtgt tatttttttc tttaacatcc
     6121 ctaaaatttt ccttacatgt tttactagcc agatttttcc tcctctcctg actactccca
     6181 gtcatagctg tccctcttct cttatggaga tccctcgact gcattaatga atcggccaac
     6241 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     6301 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     6361 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     6421 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     6481 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     6541 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     6601 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     6661 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     6721 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     6781 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     6841 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     6901 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     6961 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     7021 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     7081 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     7141 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     7201 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     7261 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     7321 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     7381 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     7441 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     7501 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     7561 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     7621 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     7681 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     7741 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     7801 ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca catagcagaa
     7861 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     7921 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     7981 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     8041 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     8101 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     8161 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     8221 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtcttcact
     8281 cgacgcggcc agcttggtcg agctggatac ttcccgtccg ccagggggac atgccggcga
     8341 tgctgaaggt cgcgcgcatt cccgatgaag aggccggtta ccgcctgttg acctggtggg
     8401 acgggcaggg cgc
//
