LOCUS       sequence-208300-        8412 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To test the effect of sequence on TDP43 splicing activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8412)
  TITLE     Rajat Rohatgi TDP43 plasmids
REFERENCE   2  (bases 1 to 8412)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHBS1337 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 G3...
FEATURES             Location/Qualifiers
     source          1..8412
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(264..283)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     533..551
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      2173..2182
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     enhancer        2335..2638
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2639..2842
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2793..2813
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      2904..2913
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2910..3611
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MGSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMAL
                     KLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARY
                     CDLPSKLGHKLN"
     misc_feature    3612..3677
                     /label=MCS
                     /note="multiple cloning site"
     CDS             3702..3758
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     protein_bind    complement(5009..5033)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     intron          5061..5633
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(5765..5784)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5830..5885
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5884..5903)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6283..6300)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6454..7042)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6534..6553)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7213..8004,8005..8073))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7836..7855
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8074..8178)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8246..8264
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gtccatgccg ccggtggagt ggcggccctc ggcgcgttcg tactgttcca cgatggtgta
       61 gtcctcgttg tgggaggtga tgtccaactt gatgttgacg ttgtaggcgc cgggcagctg
      121 cacgggcttc ttggccttgt aggtggtctt gacctcagcg tcgtagtggc cgccgtcctt
      181 cagcttcagc ctctgcttga tctcgccctt cagggcgccg tcctcggggt acatccgctc
      241 ggaggaggcc tcccagccca tggtcttctt ctgcattacg gggccgtcgg aggggaagtt
      301 ggtgccgcgc agcttcacct tgtagatgaa ctcgccgtcc tgcagggagg agtcctgggt
      361 cacggtcacc acgccgccgt cctcgaagtt catcacgcgc tcccacttga agccctcggg
      421 gaaggacagc ttcaagtagt cggggatgtc ggcggggtgc ttcacgtagg ccttggagcc
      481 gtacatgaac tgaggggaca ggatgtccca ggcgaagggc agggggccac ccttggtcac
      541 cttcagcttg gcggtctggg tgccctcgta ggggcggccc tgaggaaaaa aaaggaaagg
      601 gcatgctcgc catgtgcaag atacagtgtt gaatgtggtg caaaatataa agatgtggaa
      661 gaaaaagcca ctgaaaataa tatgaggaaa taaaaaacta gagtaaatta tcagtggaac
      721 aatttctatt atacaagaca ctacacccat acattctcct aatgctcatg taagaatttc
      781 tccaaaaata ccttccagca ctacaaacta gaaaaaaaaa gagacatgga caccaaatta
      841 agttcttaat agtgaagaac aaaagaacta ccttgcctgc tccagtggat ccagcaaccg
      901 ccaacaactg tcctctttct atcttgaaat taatatcttt caggacagga gtaccaagaa
      961 gtgagaaatt actgaagaag aggctgtcat caccattaga agtttttcta ttgttattgt
     1021 tttgttttgc tttctcaaat tattccccaa atccctgtta aaaaaaaaca cacacacaca
     1081 cacacacaca cacatcaaaa ataaaagatg agtttgtcag atattttcaa tagatgcttg
     1141 ttttatgtac catactcacc tcgccctcgc cctcgatctc gaactcgtgg ccgttcacgg
     1201 agccctccat gtgcaccttg aagcgcatga actccttgat gatggccatg ttatcctcct
     1261 cgcccttgct ggttccggtg cctccacttt catcttcacc tttagtgata ccggctgccg
     1321 tcacgaactc cttcaggacc atgtgatcgc gcttctcgtt cgggtctttg ctcagcgtgg
     1381 tctgggtgct cagatagtgg ttgtccggca ggcgcaccgg gccgtcgccg atcggggtgt
     1441 tctgctgttt gtggtcggct tcctgctggc tgccgtcctc aacgttgtgg cggatcttga
     1501 agttagcctt gatgccgttc ttctgcttgt cggcttcgat acggacgttg tggctgttgt
     1561 ggttgtactc cagcttgtga cccaggatgt tgccgtcctc cttgaagtcg ttgcccttca
     1621 gctcgatgcg gttcaccagg gtgtcgccct cgaacttcac ctccgcgcgg gtcttgtagg
     1681 tgccgtcgtc cttgaactcg atggtgcgct cctggacgta gccttccggc atggcggact
     1741 tgaagaagtc gtgacgcttc atgtggtccg ggtagcggct gaagcactgc acgccgtagg
     1801 tcagggtggt cacgagggtc ggccacggca ccggcagctt gccggtggtg cagatgaact
     1861 tcagggtcag cttgccctcg gtggcatcgc cctcgccctc gccacgcacg ctgaacttgt
     1921 ggccgtttac gtcgccgtcc agctcgactt tgataggcac agtacctgta aatagttcct
     1981 ctcctttact ggtaccatca tcagggaatg agcttacatt tctcctcttc agcatctggt
     2041 catccttctt agctttcctc agctccacat tgacctctat tctgcgacgc ctcatttctg
     2101 tactgtcttt tcccttgttc ttgaatctgt gaagacgggc agctggtgta ttagcattct
     2161 cgttggtgga tcccatggtg gcaccggtga attctccagg cgatctgacg gttcactaaa
     2221 cgagctctgc ttatataggc ctcccaccgt acacgccacc tcgacatact cgagtagtta
     2281 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
     2341 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
     2401 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
     2461 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
     2521 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
     2581 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
     2641 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
     2701 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
     2761 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
     2821 ggaggtctat ataagcagag ctggtttagt gaaccgtcag atccgctagg gatcctctag
     2881 tcagctgacg cgttaccacc ggtgccacca tgggaagcga gctgattaag gagaacatgc
     2941 acatgaagct gtacatggag ggcaccgtgg acaaccatca cttcaagtgc acatccgagg
     3001 gcgaaggcaa gccctacgag ggcacccaga ccatgagaat caaggtggtc gagggcggcc
     3061 ctctcccctt cgccttcgac atcctggcta ctagcttcct ctacggcagc aagaccttca
     3121 tcaaccacac ccagggcatc cccgacttct tcaagcagtc cttccctgag ggcttcacat
     3181 gggagagagt caccacatac gaagacgggg gcgtgctgac cgctacccag gacaccagcc
     3241 tccaggacgg ctgcctcatc tacaacgtca agatcagagg ggtgaacttc acatccaacg
     3301 gccctgtgat gcagaagaaa acactcggct gggaggcctt caccgagacg ctgtaccccg
     3361 ctgacggcgg cctggaaggc agaaacgaca tggccctgaa gctcgtgggc gggagccatc
     3421 tgatcgcaaa cgccaagacc acatatagat ccaagaaacc cgctaagaac ctcaagatgc
     3481 ctggcgtcta ctatgtggac tacagactgg aaagaatcaa ggaggccaac aacgagacct
     3541 acgtcgagca gcacgaggtg gcagtggcca gatactgcga cctccctagc aaactggggc
     3601 acaagcttaa ttccggactc agatctcgag ctcaagcttc gaattctgca gtcgacggta
     3661 ccgcgggccc gggatccacc ggatctagag gaggaagcgg agctactaac ttcagcctgc
     3721 tgaagcaggc tggagacgtg gaggagaacc ctggacctat gtctgaatat attcgggtaa
     3781 ccgaagatga gaacgatgag cccattgaaa taccatcgga agacgatggg acggtgctgc
     3841 tctccacggt tacagcccag tttccagggg cgtgtgggct tcgctacagg aatccagtgt
     3901 ctcagtgtat gagaggtgtc cggctggtag aaggaattct gcatgcccca gatgctggct
     3961 ggggaaatct ggtgtatgtt gtcaactatc caaaagataa caaaagaaaa atggatgaga
     4021 cagatgcttc atcagcagtg aaagtgaaaa gagcagtcca gaaaacatcc gatttaatag
     4081 tgttgggtct cccatggaaa acaaccgaac aggacctgaa agagtatttt agtacctttg
     4141 gagaagttct tatggtgcag gtcaagaaag atcttaagac tggtcattca aaggggtttg
     4201 gctttgttcg ttttacggaa tatgaaacac aagtgaaagt aatgtcacag cgacatatga
     4261 tagatggacg atggtgtgac tgcaaacttc ctaattctaa gcaaagccaa gatgagcctt
     4321 tgagaagcag aaaagtgttt gtggggcgct gtacagagga catgactgag gatgagctgc
     4381 gggagttctt ctctcagtac ggggatgtga tggatgtctt catccccaag ccattcaggg
     4441 cctttgcctt tgttacattt gcagatgatc agattgcgca gtctctttgt ggagaggact
     4501 tgatcattaa aggaatcagc gttcatatat ccaatgccga acctaagcac aatagcaata
     4561 gacagttaga aagaagtgga agatttggtg gtaatccagg tggctttggg aatcagggtg
     4621 gatttggtaa tagcagaggg ggtggagctg gtttgggaaa caatcaaggt agtaatatgg
     4681 gtagtgggat gaactttggt gcgttcagca ttaatccagc catgatggct gccgcccagg
     4741 cagcactaca gagcagttgg ggtatgatgg gcatgttagc cagccagcag aaccagtcag
     4801 gcccatcggg taataaccaa aaccaaggca acatgcagag ggagccaaac caggccttcg
     4861 gttctggaaa taactcttat agtggctcta attctggtgc agcaattggt tggggatcag
     4921 catccaatgc agggtcgggc agtggtttta atggaggctt tggctcaagc atggattcta
     4981 agtcttctgg ctggggaatg tagactagac ccagctttct tgtacaaagt ggttgatcct
     5041 ctagagctga gaacttcagg gtgagtttgg ggacccttga ttgttctttc tttttcgcta
     5101 ttgtaaaatt catgttatat ggagggggca aagttttcag ggtgttgttt agaatgggaa
     5161 gatgtccctt gtatcaccat ggaccctcat gataattttg tttctttcac tttctactct
     5221 gttgacaacc attgtctcct cttattttct tttcattttc tgtaactttt tcgttaaact
     5281 ttagcttgca tttgtaacga atttttaaat tcacttttgt ttatttgtca gattgtaagt
     5341 actttctcta atcacttttt tttcaaggca atcagggtat attatattgt acttcagcac
     5401 agttttagag aacaattgtt ataattaaat gataaggtag aatatttctg catataaatt
     5461 ctggctggcg tggaaatatt cttattggta gaaacaacta caccctggtc atcatcctgc
     5521 ctttctcttt atggttacaa tgatatacac tgtttgagat gaggataaaa tactctgagt
     5581 ccaaaccggg cccctctgct aaccatgttc atgccttctt ctctttccta cagctcctgg
     5641 gcaacgtgct ggttgttgtg ctgtctcatc attttggcaa agaattcact cctcaggtgc
     5701 aggctgccta tcagaaggtg gtggctggtg tggccaatgc cctggctcac aaataccact
     5761 gagatctttt tccctctgcc aaaaattatg gggacatcat gaagcccctt gagcatctga
     5821 cttctggcta ataaaggaaa tttattttca ttgcaatagt gtgttggaat tttttgtgtc
     5881 tctcactcgg aaggacatat gggagggcaa atcatttaaa acatcagaat gagtatttgg
     5941 tttagagttt ggcaacatat gcccatatgc tggctgccat gaacaaaggt tggctataaa
     6001 gaggtcatca gtatatgaaa cagccccctg ctgtccattc cttattccat agaaaagcct
     6061 tgacttgagg ttagattttt tttatatttt gttttgtgtt atttttttct ttaacatccc
     6121 taaaattttc cttacatgtt ttactagcca gatttttcct cctctcctga ctactcccag
     6181 tcatagctgt ccctcttctc ttatggagat ccctcgactg cattaatgaa tcggccaacg
     6241 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     6301 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     6361 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     6421 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     6481 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     6541 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     6601 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     6661 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     6721 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     6781 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     6841 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     6901 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     6961 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     7021 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     7081 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     7141 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     7201 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     7261 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     7321 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     7381 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     7441 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     7501 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     7561 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     7621 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     7681 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     7741 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     7801 gcgaccgagt tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac
     7861 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     7921 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     7981 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     8041 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     8101 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     8161 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat
     8221 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcactc
     8281 gacgcggcca gcttggtcga gctggatact tcccgtccgc cagggggaca tgccggcgat
     8341 gctgaaggtc gcgcgcattc ccgatgaaga ggccggttac cgcctgttga cctggtggga
     8401 cgggcagggc gc
//
