LOCUS       sequence-208331-       14559 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Binary vectors used for the inducible expression of tripartite 
            split-sfGFP components (with a 3xHA tag) in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14559)
  AUTHORS   Liu TY, Chou WC, Chen WY, Chu CY, Dai CY, Wu PY
  TITLE     Detection of membrane protein-protein interaction in planta based on
            dual intein-coupled tripartite split-GFP association.
  JOURNAL   Plant J. 2018 Feb 16. doi: 10.1111/tpj.13874.
  PUBMED    29451720
REFERENCE   2  (bases 1 to 14559)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2018 Feb 16. doi: 10.1111/tpj.13874."
COMMENT     Sequence Label: UBQ10:sXVE:(MCS)-3xHA-S11
FEATURES             Location/Qualifiers
     source          1..14559
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(28..831)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGAWIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(139..158)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     749..768
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(881..1060)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        4322..4368
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     CDS             4449..4538
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      4660..4912
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    5080..5104
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5569..6219
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     primer_bind     complement(6407..6424)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(6658..6677)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     oriT            6900..7009
                     /note="incP origin of transfer"
     CDS             7042..7413
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     rep_origin      7540..8251
                     /label=oriV
                     /note="incP origin of replication"
     mobile_element  8873..9640
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     CDS             9832..10626
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     CDS             10925..12073
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    13669..13693
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     complement(13940..13961)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     14152..14171
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     14296..14318
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     14310..14327
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     14311..14327
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(14340..14540)
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
ORIGIN
        1 atccgtcgaa gctagcttgg gtcccgctca gaagaactcg tcaagaaggc gatagaaggc
       61 gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg aggaagcggt cagcccattc
      121 gccgccaagc tcttcagcaa tatcacgggt agccaacgct atgtcctgat agcggtccgc
      181 cacacccagc cggccacagt cgatgaatcc agaaaagcgg ccattttcca ccatgatatt
      241 cggcaagcag gcatcgccat gggtcacgac gagatcctcg ccgtcgggca tgcgcgcctt
      301 gagcctggcg aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg
      361 atcgacaaga ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg
      421 gtcgaatggg caggtagccg gatcaagcgt atgcagccgc cgcattgcat cagccatgat
      481 ggatactttc tcggcaggag caaggtgaga tgacaggaga tcctgccccg gcacttcgcc
      541 caatagcagc cagtcccttc ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac
      601 gcccgtcgtg gccagccacg atagccgcgc tgcctcgtcc tgcagttcat tcagggcacc
      661 ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc
      721 ggcatcagag cagccgattg tctgttgtgc ccagtcatag ccgaatagcc tctccaccca
      781 agcggccgga gaacctgcgt gcaatccatc ttgttcaatc caagctccca tgggccctcg
      841 actagagtcg agatctggat tgagagtgaa tatgagactc taattggata ccgaggggaa
      901 tttatggaac gtcagtggag catttttgac aagaaatatt tgctagctga tagtgacctt
      961 aggcgacttt tgaacgcgca ataatggttt ctgacgtatg tgcttagctc attaaactcc
     1021 agaaacccgc ggctgagtgg ctccttcaac gttgcggttc tgtcagttcc aaacgtaaaa
     1081 cggcttgtcc cgcgtcatcg gcgggggtca taacgtgact cccttaattc tccgctcatg
     1141 atcttgatcc cctgcgccat cagatccttg gcggcaagaa agccatccag tttactttgc
     1201 agggcttccc aaccttacca gagggcgccc cagctggcaa ttccggttcg cttgctgtcc
     1261 ataaaaccgc ccagtctagc tatcgccatg taagcccact gcaagctacc tgctttctct
     1321 ttgcgcttgc gttttccctt gtccagatag cccagtagct gacattcatc cggggtcagc
     1381 accgtttctg cggactggct ttctacgtgt tccgcttcct ttagcagccc ttgcgccctg
     1441 agtgcttgcg gcagcgtgaa gcttcgacga gtcagtaata aacggcgtca aagtggttgc
     1501 agccggcaca cacgagtcgt gtttatcaac tcaaagcaca aatacttttc ctcaacctaa
     1561 aaataaggca attagccaaa aacaactttg cgtgtaaaca acgctcaata cacgtgtcat
     1621 tttattatta gctattgctt caccgcctta gctttctcgt gacctagtcg tcctcgtctt
     1681 ttcttcttct tcttctataa aacaataccc aaagagctct tcttcttcac aattcagatt
     1741 tcaatttctc aaaatcttaa aaactttctc tcaattctct ctaccgtgat caaggtaaat
     1801 ttctgtgttc cttattctct caaaatcttc gattttgttt tcgttcgatc ccaatttcgt
     1861 atatgttctt tggtttagat tctgttaatc ttagatcgaa gacgattttc tgggtttgat
     1921 cgttagatat catcttaatt ctcgattagg gtttcataga tatcatccga tttgttcaaa
     1981 taatttgagt tttgtcgaat aattactctt cgatttgtga tttctatcta gttctggtgt
     2041 tagtttctag tttgtgcgat cgaatttgtc gattaatctg agtttttctg attaacagac
     2101 tagcccgcgg aaaatgaagg ctcttactgc tagacaacaa gaagtttttg atcttattag
     2161 agatcatatt tctcaaactg gaatgcctcc tactagagct gaaattgctc aaagacttgg
     2221 atttaggtct cctaatgctg ctgaagaaca tcttaaggct cttgctagaa agggagttat
     2281 tgaaattgtt tctggagctt caagaggaat tagacttctt caagaagaag aagaaggact
     2341 tcctcttgtt ggaagagttg ctgctggaga accttcttct gctcctccta ctgatgtttc
     2401 tcttggagat gaacttcatc ttgatggaga agatgttgct atggctcatg ctgatgctct
     2461 tgatgatttt gatcttgata tgcttggaga tggagattct cctggacctg gatttactcc
     2521 tcatgattct gctccttatg gagcacttga tatggctgat tttgaatttg aacaaatgtt
     2581 tactgatgct cttggaattg atgaatatgg aggagatcct tctgctggag atatgagagc
     2641 tgctaatctt tggccttctc ctcttatgat taagaggtct aagaagaact ctcttgctct
     2701 ttctcttact gctgaccaaa tggtttctgc tcttcttgat gctgaacctc ctattcttta
     2761 ttctgaatat gatcctacta gacctttttc tgaggcttct atgatgggac ttcttactaa
     2821 tcttgctgat agagaacttg ttcatatgat taattgggct aagagagttc ctggatttgt
     2881 tgatcttact cttcatgacc aagttcatct tcttgaatgt gcttggcttg aaattcttat
     2941 gattggactt gtttggaggt ctatggaaca tcctgttaaa cttctttttg ctcctaatct
     3001 tcttcttgat agaaatcaag gaaagtgtgt tgaaggaatg gttgaaattt ttgatatgct
     3061 tcttgctact tcttcaagat ttagaatgat gaatcttcaa ggagaagaat ttgtttgtct
     3121 taagtctatt attcttctta attctggagt ttatactttt ctttcttcta ctcttaagtc
     3181 tcttgaagaa aaggatcata ttcatagagt tcttgataag attactgata ctcttattca
     3241 tcttatggct aaggctggac ttactcttca acaacaacat caaagacttg ctcaacttct
     3301 tcttattctt tctcatatta gacatatgtc taataaggga atggaacatc tttattctat
     3361 gaagtgtaag aatgttgttc ctctttatga tcttcttctt gaaatgcttg atgctcatag
     3421 acttcatgct cctacttcaa gaggaggagc ttctgttgaa gaaactgacc aatctcatct
     3481 tgctactgct ggatctactt cttctcattc tcttcaaaag tattatatta ctggagaagc
     3541 tgaaggattt cctgctactg tttgaagatc ttgatcaagc tccctggcca attcccagag
     3601 atgttagctg aaatcatcac taatcagata ccaaaatatt caaatggaaa tatcaaatag
     3661 cttctgtttc atcaaaaatg actcgaccta actgagtaag catgcttgtt cgagtattat
     3721 ggcattggga aaactgtttt tcttgtacca tttgttgtgc ttgtaattta ctgtgttttt
     3781 tattcggttt tcgctatcga actgtgaaat ggaaatggat ggagaagagt taatgaatga
     3841 tatggtcctt ttgttcattc tcaaattaat attatttgtt ttttctctta tttgttgtgt
     3901 gttgaatttg aaattataag agatatgcaa acattttgtt ttgagtaaaa atgtgtcaaa
     3961 tcgtggcctc taatgaccga agttaatatg aggagtaaaa catcccaaac gcggccgcaa
     4021 ccggtcttgc atccagctgg gctgcaggtc gaggctaaaa aactaatcgc attatcatcc
     4081 cctcgacgta ctgtacatat aaccactggt tttatataca gcagtactgt acatataacc
     4141 actggtttta tatacagcag tcgacgtact gtacatataa ccactggttt tatatacagc
     4201 agtactgtac atataaccac tggttttata tacagcagtc gaggtaagat tagatatgga
     4261 tatgtatatg gatatgtata tggtggtaat gccatgtaat atgctcgact ctaggatctt
     4321 cgcaagaccc ttcctctata taaggaagtt catttcattt ggagaggaca cgctgaagct
     4381 agtcgactct agccaaatct aggggatccg gcgcgcccgg gttaattaac tcgagtctag
     4441 agtcgactta cccatacgat gttccagatt acgctggcta cccatacgat gttccagatt
     4501 acgctggatc atacccatac gatgttccag attacgctac tagtggtgga gggtctggtg
     4561 gcggatcaac cagtgaaaag cgtgaccaca tggtccttct tgagtatgta actgctgctg
     4621 ggattacaga tgctagctaa atcgagctcg aatttccccg atcgttcaaa catttggcaa
     4681 taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg
     4741 ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg
     4801 gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag
     4861 cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcggggaatt
     4921 cagatcggct gagtggctcc ttcaacgttg cggttctgtc agttccaaac gtaaaacggc
     4981 ttgtcccgcg tcatcggcgg gggtcataac gtgactccct taattctccg ctcatgatca
     5041 gattgtcgtt tcccgccttc agtttaaact atcagtgttt gacaggatat attggcgggt
     5101 aaacctaaga gaaaagagcg tttattagaa taatcggata tttaaaaggg cgtgaaaagg
     5161 tttatccgtt cgtccatttg tatgtgcatg ccaaccacag ggttccccag atctggcgcc
     5221 ggccagcgag acgagcaaga ttggccgccg cccgaaacga tccgacagcg cgcccagcac
     5281 aggtgcgcag gcaaattgca ccaacgcata cagcgccagc agaatgccat agtgggcggt
     5341 gacgtcgttc gagtgaacca gatcgcgcag gaggcccggc agcaccggca taatcaggcc
     5401 gatgccgaca gcgtcgagcg cgacagtgct cagaattacg atcaggggta tgttgggttt
     5461 cacgtctggc ctccggacca gcctccgctg gtccgattga acgcgcggat tctttatcac
     5521 tgataagttg gtggacatat tatgtttatc agtgataaag tgtcaagcat gacaaagttg
     5581 cagccgaata cagtgatccg tgccgccctg gacctgttga acgaggtcgg cgtagacggt
     5641 ctgacgacac gcaaactggc ggaacggttg ggggttcagc agccggcgct ttactggcac
     5701 ttcaggaaca agcgggcgct gctcgacgca ctggccgaag ccatgctggc ggagaatcat
     5761 acgcattcgg tgccgagagc cgacgacgac tggcgctcat ttctgatcgg gaatgcccgc
     5821 agcttcaggc aggcgctgct cgcctaccgc gatggcgcgc gcatccatgc cggcacgcga
     5881 ccgggcgcac cgcagatgga aacggccgac gcgcagcttc gcttcctctg cgaggcgggt
     5941 ttttcggccg gggacgccgt caatgcgctg atgacaatca gctacttcac tgttggggcc
     6001 gtgcttgagg agcaggccgg cgacagcgat gccggcgagc gcggcggcac cgttgaacag
     6061 gctccgctct cgccgctgtt gcgggccgcg atagacgcct tcgacgaagc cggtccggac
     6121 gcagcgttcg agcagggact cgcggtgatt gtcgatggat tggcgaaaag gaggctcgtt
     6181 gtcaggaacg ttgaaggacc gagaaagggt gacgattgat caggaccgct gccggagcgc
     6241 aacccactca ctacagcaga gccatgtaga caacatcccc tccccctttc caccgcgtca
     6301 gacgcccgta gcagcccgct acgggctttt tcatgccctg ccctagcgtc caagcctcac
     6361 ggccgcgctc ggcctctctg gcggccttct ggcgctcttc cgcttcctcg ctcactgact
     6421 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     6481 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     6541 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     6601 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     6661 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     6721 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgcttttc cgctgcataa
     6781 ccctgcttcg gggtcattat agcgattttt tcggtatatc catccttttt cgcacgatat
     6841 acaggatttt gccaaagggt tcgtgtagac tttccttggt gtatccaacg gcgtcagccg
     6901 ggcaggatag gtgaagtagg cccacccgcg agcgggtgtt ccttcttcac tgtcccttat
     6961 tcgcacctgg cggtgctcaa cgggaatcct gctctgcgag gctggccggc taccgccggc
     7021 gtaacagatg agggcaagcg gatggctgat gaaaccaagc caaccaggaa gggcagccca
     7081 cctatcaagg tgtactgcct tccagacgaa cgaagagcga ttgaggaaaa ggcggcggcg
     7141 gccggcatga gcctgtcggc ctacctgctg gccgtcggcc agggctacaa aatcacgggc
     7201 gtcgtggact atgagcacgt ccgcgagctg gcccgcatca atggcgacct gggccgcctg
     7261 ggcggcctgc tgaaactctg gctcaccgac gacccgcgca cggcgcggtt cggtgatgcc
     7321 acgatcctcg ccctgctggc gaagatcgaa gagaagcagg acgagcttgg caaggtcatg
     7381 atgggcgtgg tccgcccgag ggcagagcca tgactttttt agccgctaaa acggccgggg
     7441 ggtgcgcgtg attgccaagc acgtccccat gcgctccatc aagaagagcg acttcgcgga
     7501 gctggtgaag tacatcaccg acgagcaagg caagaccgag cgcctttccg acgctcaccg
     7561 ggctggttgc cctcgccgct gggctggcgg ccgtctatgg ccctgcaaac gcgccagaaa
     7621 cgccgtcgaa gccgtgtgcg agacaccgcg gccgccggcg ttgtggatac ctcgcggaaa
     7681 acttggccct cactgacaga tgaggggcgg acgttgacac ttgaggggcc gactcacccg
     7741 gcgcggcgtt gacagatgag gggcaggctc gatttcggcc ggcgacgtgg agctggccag
     7801 cctcgcaaat cggcgaaaac gcctgatttt acgcgagttt cccacagatg atgtggacaa
     7861 gcctggggat aagtgccctg cggtattgac acttgagggg cgcgactact gacagatgag
     7921 gggcgcgatc cttgacactt gaggggcaga gtgctgacag atgaggggcg cacctattga
     7981 catttgaggg gctgtccaca ggcagaaaat ccagcatttg caagggtttc cgcccgtttt
     8041 tcggccaccg ctaacctgtc ttttaacctg cttttaaacc aatatttata aaccttgttt
     8101 ttaaccaggg ctgcgccctg tgcgcgtgac cgcgcacgcc gaaggggggt gccccccctt
     8161 ctcgaaccct cccggcccgc taacgcgggc ctcccatccc cccaggggct gcgcccctcg
     8221 gccgcgaacg gcctcacccc aaaaatggca gcgctggcag tccttgccat tgccgggatc
     8281 ggggcagtaa cgggatgggc gatcagcccg agcgcgacgc ccggaagcat tgacgtgccg
     8341 caggtgctgg catcgacatt cagcgaccag gtgccgggca gtgagggcgg cggcctgggt
     8401 ggcggcctgc ccttcacttc ggccgtcggg gcattcacgg acttcatggc ggggccggca
     8461 atttttacct tgggcattct tggcatagtg gtcgcgggtg ccgtgctcgt gttcgggggt
     8521 gcgataaacc cagcgaacca tttgaggtga taggtaagat tataccgagg tatgaaaacg
     8581 agaattggac ctttacagaa ttactctatg aagcgccata tttaaaaagc taccaagacg
     8641 aagaggatga agaggatgag gaggcagatt gccttgaata tattgacaat actgataaga
     8701 taatatatct tttatataga agatatcgcc gtatgtaagg atttcagggg gcaaggcata
     8761 ggcagcgcgc ttatcaatat atctatagaa tgggcaaagc ataaaaactt gcatggacta
     8821 atgcttgaaa ccaggacaat aaccttatag cttgtaaatt ctatcataat tgggtaatga
     8881 ctccaactta ttgatagtgt tttatgttca gataatgccc gatgactttg tcatgcagct
     8941 ccaccgattt tgagaacgac agcgacttcc gtcccagccg tgccaggtgc tgcctcagat
     9001 tcaggttatg ccgctcaatt cgctgcgtat atcgcttgct gattacgtgc agctttccct
     9061 tcaggcggga ttcatacagc ggccagccat ccgtcatcca tatcaccacg tcaaagggtg
     9121 acagcaggct cataagacgc cccagcgtcg ccatagtgcg ttcaccgaat acgtgcgcaa
     9181 caaccgtctt ccggagactg tcatacgcgt aaaacagcca gcgctggcgc gatttagccc
     9241 cgacatagcc ccactgttcg tccatttccg cgcagacgat gacgtcactg cccggctgta
     9301 tgcgcgaggt taccgactgc ggcctgagtt ttttaagtga cgtaaaatcg tgttgaggcc
     9361 aacgcccata atgcgggctg ttgcccggca tccaacgcca ttcatggcca tatcaatgat
     9421 tttctggtgc gtaccgggtt gagaagcggt gtaagtgaac tgcagttgcc atgttttacg
     9481 gcagtgagag cagagatagc gctgatgtcc ggcggtgctt ttgccgttac gcaccacccc
     9541 gtcagtagct gaacaggagg gacagctgat agaaacagaa gccactggag cacctcaaaa
     9601 acaccatcat acactaaatc agtaagttgg cagcatcacc cataattgtg gtttcaaaat
     9661 cggctccgtc gatactatgt tatacgccaa ctttgaaaac aactttgaaa aagctgtttt
     9721 ctggtattta aggttttaga atgcaaggaa cagtgaattg gagttcgtct tgttataatt
     9781 agcttcttgg ggtatcttta aatactgtag aaaagaggaa ggaaataata aatggctaaa
     9841 atgagaatat caccggaatt gaaaaaactg atcgaaaaat accgctgcgt aaaagatacg
     9901 gaaggaatgt ctcctgctaa ggtatataag ctggtgggag aaaatgaaaa cctatattta
     9961 aaaatgacgg acagccggta taaagggacc acctatgatg tggaacggga aaaggacatg
    10021 atgctatggc tggaaggaaa gctgcctgtt ccaaaggtcc tgcactttga acggcatgat
    10081 ggctggagca atctgctcat gagtgaggcc gatggcgtcc tttgctcgga agagtatgaa
    10141 gatgaacaaa gccctgaaaa gattatcgag ctgtatgcgg agtgcatcag gctctttcac
    10201 tccatcgaca tatcggattg tccctatacg aatagcttag acagccgctt agccgaattg
    10261 gattacttac tgaataacga tctggccgat gtggattgcg aaaactggga agaagacact
    10321 ccatttaaag atccgcgcga gctgtatgat tttttaaaga cggaaaagcc cgaagaggaa
    10381 cttgtctttt cccacggcga cctgggagac agcaacatct ttgtgaaaga tggcaaagta
    10441 agtggcttta ttgatcttgg gagaagcggc agggcggaca agtggtatga cattgccttc
    10501 tgcgtccggt cgatcaggga ggatatcggg gaagaacagt atgtcgagct attttttgac
    10561 ttactgggga tcaagcctga ttgggagaaa ataaaatatt atattttact ggatgaattg
    10621 ttttagtacc tagatgtggc gcaacgatgc cggcgacaag caggagcgca ccgacttctt
    10681 ccgcatcaag tgttttggct ctcaggccga ggcccacggc aagtatttgg gcaaggggtc
    10741 gctggtattc gtgcagggca agattcggaa taccaagtac gagaaggacg gccagacggt
    10801 ctacgggacc gacttcattg ccgataaggt ggattatctg gacaccaagg caccaggcgg
    10861 gtcaaatcag gaataagggc acattgcccc ggcgtgagtc ggggcaatcc cgcaaggagg
    10921 gtgaatgaat cggacgtttg accggaaggc atacaggcaa gaactgatcg acgcggggtt
    10981 ttccgccgag gatgccgaaa ccatcgcaag ccgcaccgtc atgcgtgcgc cccgcgaaac
    11041 cttccagtcc gtcggctcga tggtccagca agctacggcc aagatcgagc gcgacagcgt
    11101 gcaactggct ccccctgccc tgcccgcgcc atcggccgcc gtggagcgtt cgcgtcgtct
    11161 cgaacaggag gcggcaggtt tggcgaagtc gatgaccatc gacacgcgag gaactatgac
    11221 gaccaagaag cgaaaaaccg ccggcgagga cctggcaaaa caggtcagcg aggccaagca
    11281 ggccgcgttg ctgaaacaca cgaagcagca gatcaaggaa atgcagcttt ccttgttcga
    11341 tattgcgccg tggccggaca cgatgcgagc gatgccaaac gacacggccc gctctgccct
    11401 gttcaccacg cgcaacaaga aaatcccgcg cgaggcgctg caaaacaagg tcattttcca
    11461 cgtcaacaag gacgtgaaga tcacctacac cggcgtcgag ctgcgggccg acgatgacga
    11521 actggtgtgg cagcaggtgt tggagtacgc gaagcgcacc cctatcggcg agccgatcac
    11581 cttcacgttc tacgagcttt gccaggacct gggctggtcg atcaatggcc ggtattacac
    11641 gaaggccgag gaatgcctgt cgcgcctaca ggcgacggcg atgggcttca cgtccgaccg
    11701 cgttgggcac ctggaatcgg tgtcgctgct gcaccgcttc cgcgtcctgg accgtggcaa
    11761 gaaaacgtcc cgttgccagg tcctgatcga cgaggaaatc gtcgtgctgt ttgctggcga
    11821 ccactacacg aaattcatat gggagaagta ccgcaagctg tcgccgacgg cccgacggat
    11881 gttcgactat ttcagctcgc accgggagcc gtacccgctc aagctggaaa ccttccgcct
    11941 catgtgcgga tcggattcca cccgcgtgaa gaagtggcgc gagcaggtcg gcgaagcctg
    12001 cgaagagttg cgaggcagcg gcctggtgga acacgcctgg gtcaatgatg acctggtgca
    12061 ttgcaaacgc tagggccttg tggggtcagt tccggctggg ggttcagcag ccagcgcttt
    12121 actggcattt caggaacaag cgggcactgc tcgacgcact tgcttcgctc agtatcgctc
    12181 gggacgcacg gcgcgctcta cgaactgccg ataaacagag gattaaaatt gacaattgtg
    12241 attaaggctc agattcgacg gcttggagcg gccgacgtgc aggatttccg cgagatccga
    12301 ttgtcggccc tgaagaaagc tccagagatg ttcgggtccg tttacgagca cgaggagaaa
    12361 aagcccatgg aggcgttcgc tgaacggttg cgagatgccg tggcattcgg cgcctacatc
    12421 gacggcgaga tcattgggct gtcggtcttc aaacaggagg acggccccaa ggacgctcac
    12481 aaggcgcatc tgtccggcgt tttcgtggag cccgaacagc gaggccgagg ggtcgccggt
    12541 atgctgctgc gggcgttgcc ggcgggttta ttgctcgtga tgatcgtccg acagattcca
    12601 acgggaatct ggtggatgcg catcttcatc ctcggcgcac ttaatatttc gctattctgg
    12661 agcttgttgt ttatttcggt ctaccgcctg ccgggcgggg tcgcggcgac ggtaggcgct
    12721 gtgcagccgc tgatggtcgt gttcatctct gccgctctgc taggtagccc gatacgattg
    12781 atggcggtcc tgggggctat ttgcggaact gcgggcgtgg cgctgttggt gttgacacca
    12841 aacgcagcgc tagatcctgt cggcgtcgca gcgggcctgg cgggggcggt ttccatggcg
    12901 ttcggaaccg tgctgacccg caagtggcaa cctcccgtgc ctctgctcac ctttaccgcc
    12961 tggcaactgg cggccggagg acttctgctc gttccagtag ctttagtgtt tgatccgcca
    13021 atcccgatgc ctacaggaac caatgttctc ggcctggcgt ggctcggcct gatcggagcg
    13081 ggtttaacct acttcctttg gttccggggg atctcgcgac tcgaacctac agttgtttcc
    13141 ttactgggct ttctcagccc cagatctggg gtcgatcagc cggggatgca tcaggccgac
    13201 agtcggaact tcgggtcccc gacctgtacc attcggtgag caatggatag gggagttgat
    13261 atcgtcaacg ttcacttcta aagaaatagc gccactcagc ttcctcagcg gctttatcca
    13321 gcgatttcct attatgtcgg catagttctc aagatcgaca gcctgtcacg gttaagcgag
    13381 aaatgaataa gaaggctgat aattcggatc tctgcgaggg agatgatatt tgatcacagg
    13441 cagcaacgct ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc atccgtgttt
    13501 caaacccggc agcttagttg ccgttcttcc gaatagcatc ggtaacatga gcaaagtctg
    13561 ccgccttaca acggctctcc cgctgacgcc gtcccggact gatgggctgc ctgtatcgag
    13621 tggtgatttt gtgccgagct gccggtcggg gagctgttgg ctggctggtg gcaggatata
    13681 ttgtggtgta aacaaattga cgcttagaca acttaataac acattgcgga cgtttttaat
    13741 gtactggggt ggtttttctt ttcaccagtg agacgggcaa cagctgattg cccttcaccg
    13801 cctggccctg agagagttgc agcaagcggt ccacgctggt ttgccccagc aggcgaaaat
    13861 cctgtttgat ggtggttccg aaatcggcaa aatcccttat aaatcaaaag aatagcccga
    13921 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
    13981 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
    14041 caaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
    14101 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
    14161 agcgaaagga gcgggcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
    14221 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
    14281 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa ttaattccca
    14341 tcttgaaaga aatatagttt aaatatttat tgataaaata acaagtcagg tattatagtc
    14401 caagcaaaaa cataaattta ttgatgcaag tttaaattca gaaatatttc aataactgat
    14461 tatatcagct ggtacattgc cgtagatgaa agactgagtg cgatattatg tgtaatacat
    14521 aaattgatga tatagctagc ttagctcatc gggggatcg
//
