LOCUS       sequence-208512-        3525 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PdR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Iwata F, Hirakawa H, Nagamune T
  TITLE     A Stable Artificial Multienzymatic Complex Using a Heterotrimeric 
            Protein From Metallosphaera sedula.
  JOURNAL   Biotechnol J. 2018 Nov;13(11):e1700662. doi: 10.1002/biot.201700662.
            Epub 2018 Apr 30.
  PUBMED    29663675
REFERENCE   2  (bases 1 to 3525)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J."; date: "2018-11"; volume: "13(11):e1700662. doi"; pages: " 
            10.1002/biot.201700662. Epub 2018 Apr 30"
COMMENT     Sequence Label: pBsaGSPdR
FEATURES             Location/Qualifiers
     source          1..3525
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(1..6,7..24,25..31)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    39..55
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     44..66
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     63..79
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     63..79
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1446..1463)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1446..1462)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1455..1477)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(1617..1634)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1788..2376)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1868..1887)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2519..2621
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             2622..3281
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2688..2707)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     protein_bind    3490..3511
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
       61 cacaggaaac agctatgacc atgattacga attcgagctc gggtctcggg aggtagcatg
      121 aacgcaaacg acaacgtggt gattgttggc actggtctgg ctggcgttga agttgcgttt
      181 ggtctgcgtg caagcggctg ggaaggtaac atccgcctgg tgggtgatgc gactgttatt
      241 ccgcaccacc tgccgccgct gtctaaagcg tacctggcgg gcaaggcgac tgcggagtct
      301 ctgtacctgc gcactccgga tgcttacgcg gctcagaaca tccagctgct gggcggtacc
      361 caggttaccg cgattaaccg tgaccgtcag caggtgattc tgtctgatgg ccgtgcactg
      421 gattatgatc gtctggtgct ggcaactggc ggtcgtccgc gtccgctgcc ggtggctagc
      481 ggcgctgttg gtaaagcaaa caacttccgt tatctgcgta ccctggaaga cgcagaatgc
      541 atccgtcgcc agctgattgc ggacaaccgt ctggttgtta tcggtggcgg ctacatcggt
      601 ctggaggttg ctgctactgc gatcaaagcg aacatgcacg tgactctgct ggacactgct
      661 gcgcgtgttc tggaacgtgt gactgctccg ccggtgagcg cattctacga gcatctgcac
      721 cgcgaagctg gtgtggacat tcgtactggt actcaggttt gcggctttga aatgtctacc
      781 gatcagcaga aagttactgc ggttctgtgc gaagacggca ctcgtctgcc ggctgatctg
      841 gttattgcgg gtattggcct gatcccgaac tgcgaactgg catccgcagc gggcctgcag
      901 gttgacaacg gcatcgtgat caacgagcac atgcagacta gcgatccgct gattatggca
      961 gtgggcgatt gtgcgcgttt ccactctcag ctgtatgacc gttgggttcg catcgaaagc
     1021 gtgccgaacg cgctggaaca ggcgcgtaaa atcgcagcaa tcctgtgcgg taaagttccg
     1081 cgtgacgagg cggctccgtg gttttggagc gaccagtatg aaatcggcct gaaaatggtg
     1141 ggcctgtccg aaggttacga ccgtatcatc gttcgtggct ctctggcgca gccggacttc
     1201 tctgttttct atctgcaggg tgatcgcgtt ctggcagttg acactgtgaa ccgtccggtg
     1261 gaatttaacc agtctaaaca gatcattact gatcgcctgc cggttgagcc gaacctgctg
     1321 ggtgacgaat ctgtgccgct gaaggagatc atcgcggctg caaaagctga actgtcttct
     1381 gcgtgaggat ctgagaccgt acccggggat cctctagagt cgacctgcag gcatgcaagc
     1441 ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     1501 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     1561 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gagcttcttc cgcttcctcg
     1621 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     1681 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     1741 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     1801 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     1861 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     1921 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     1981 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     2041 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     2101 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     2161 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     2221 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     2281 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     2341 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     2401 gggtctgacg ctcagtggaa ctccgtcgaa cggaagatca cttcgcagaa taaataaatc
     2461 ctggtgtccc tgttgatacc gggaagccct gggccaactt ttggcgaaaa tgagacgttg
     2521 atcggcacgt aagaggttcc aactttcacc ataatgaaat aagatcacta ccgggcgtat
     2581 tttttgagtt atcgagattt tcaggagcta aggaagctaa aatggagaaa aaaatcactg
     2641 gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt
     2701 cagttgctca atgtacctat aaccagaccg ttcagctgga tattacggcc tttttaaaga
     2761 ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt gcccgcctga
     2821 tgaatgctca tccggaattt cgtatggcaa tgaaagacgg tgagctggtg atatgggata
     2881 gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttca tcgctctgga
     2941 gtgaatacca cgacgatttc cggcagtttc tacacatata ttcgcaagat gtggcgtgtt
     3001 acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt ttcgtctcag
     3061 ccaatccctg ggtgagtttc accagttttg atttaaacgt ggccaatatg gacaacttct
     3121 tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc
     3181 tggcgattca ggttcatcat gccgtttgtg atggcttcca tgtcggcaga atgcttaatg
     3241 aattacaaca gtactgcgat gagtggcagg gcggggcgta atttttttaa ggcagttatt
     3301 ggtgccctta aacgcctggt tgctacgcct gaataagtga taataagcgg atgaatggca
     3361 gaaattcagc ttggcccagt gccaagctcc aatacgcaaa ccgcctctcc ccgcgcgttg
     3421 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
     3481 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggc
//
