LOCUS       sequence-208601-        4930 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses proteins pan-neuronally in C. elegans (entire nervous 
            system).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4930)
  AUTHORS   Schade MA, Reynolds NK, Dollins CM, Miller KG
  TITLE     Mutations that rescue the paralysis of Caenorhabditis elegans ric-8 
            (synembryn) mutants activate the G alpha(s) pathway and define a 
            third major branch of the synaptic signaling network.
  JOURNAL   Genetics. 2005 Feb;169(2):631-49. doi: 10.1534/genetics.104.032334. 
            Epub 2004 Oct 16.
  PUBMED    15489510
REFERENCE   2  (bases 1 to 4930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.104.032334"; journalName: "Genetics"; date: 
            "2005-02"; volume: "169"; issue: "2"; pages: "631-49"
COMMENT     Sequence Label: KG#470
FEATURES             Location/Qualifiers
     source          1..4930
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2370..2392)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2492..2511
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2589..2607)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2675..2779
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2780..2848,2849..3640)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2998..3017)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3811..4399
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4300..4319
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4553..4570
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4687..4708
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4723..4728,4729..4746,4747..4753)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4761..4777
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4766..4788
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4785..4801
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4785..4801
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgggc tgcaggatct tcagatggga gcagtggact gtctatttga tattcttaaa
       61 atgtactcta taaaagatga aactgtaatg accaattccc tacataaata tttttaacta
      121 attggtcgga tatttgggga tcagagaagt acttgttttt gcgatattaa taatgatata
      181 tttttctagt aaaaaaatgt taagcgattt gtgaataatt aagttggctt gaaaaagatt
      241 ttttgatagc tatgtatttt gttaatttaa atcagatttt aaaactttat tattgactat
      301 ttatatatat tatatattat atattttcat ggtttaatca aaatctatta aattatttct
      361 aaaatctcta aatatcgaat tctctaaaaa gcttcatcaa caaaagcatt tcaattatct
      421 aacacaatga gaataattca agaactgaaa tagaaagaaa tctagaaaaa aaagagagtg
      481 aggtgcccta ttaaatcggt gctctcctct ctaacagaag cgcctacacg cacccaagag
      541 agctcttttt ctttcttttt tcggagcaca catacacgca tacactcctc agaagtgtat
      601 agagaaaatg ggagaagaag cgaaagaaga cgaagaagcg gttgtgatgt atgcatcggg
      661 cgtttctatc tctctccgtg agcaacgagc tagtcaaccc atcgatccat ttttgtgagc
      721 acacacagag agagactcaa acacactcac agcttcacac tgagctccaa gagctctgta
      781 gagagaagcc agagtatgct ttcctcgatt ttcctaacgt cttcttcttc tgcttttttc
      841 tttttcccaa cgagttgtag tccactaact tcaatttttc ctccagagaa agagttttag
      901 agagacagct gataaataca ctagagaaat aaaaagaatg ataaaaattt gctattttaa
      961 tcagttccct ctcgtttctc taagttttgt tgttgggttg ggaaattaaa attggaaaat
     1021 ggaaataacg tttttttctg tctaatcttg aattctccag acatttctta tttccttttt
     1081 tgttgcaagt ttttcttcct tctctaattt ttaatcaaag atatctcgct atgaactttg
     1141 aatttttgat attttgattc tataaataca taaagtaatc taaatgctct tttaaaataa
     1201 atctacagta gccctatttt cagggatccc cgggattggc caaaggaccc aaaggtatgt
     1261 ttcgaatgat actaacataa catagaacat tttcaggagg acccttggct agcagctctc
     1321 ctgcagggtt cgacggtacc ggtcagatat cactcgagga gctcccgaga tcttcagcgc
     1381 tcagcattcg tagaattgta agtttaaact ttctcatact aactaacgcg ctctatttaa
     1441 attttcagaa ttccaactga gcgccggtcg ctaccattac caacttgtct ggtgtcaaaa
     1501 ataatagggg ccgctgtcat cagagtaagt ttaaactgag ttctactaac taacgagtaa
     1561 tatttaaatt ttcagcatct cgcgcccgtg cctctgactt ctaagtccaa ttactcttca
     1621 acatccctac atgctctttc tccctgtgct cccaccccct atttttgtta ttatcaaaaa
     1681 aacttcttct taatttcttt gttttttagc ttcttttaag tcacctctaa caatgaaatt
     1741 gtgtagattc aaaaatagaa ttaattcgta ataaaaagtc gaaaaaaatt gtgctccctc
     1801 cccccattaa taataattct atcccaaaat ctacacaatg ttctgtgtac acttcttatg
     1861 ttttttttac ttctgataaa ttttttttga aacatcatag aaaaaaccgc acacaaaata
     1921 ccttatcata tgttacgttt cagtttatga ccgcaatttt tatttcttcg cacgtctggg
     1981 cctctcatga cgtcaaatca tgctcatcgt gaaaaagttt tggagtattt ttggaatttt
     2041 tcaatcaagt gaaagtttat gaaattaatt ttcctgcttt tgctttttgg gggtttcccc
     2101 tattgtttgt caagagtttc gaggacggcg tttttcttgc taaaatcaca agtattgatg
     2161 agcacgatgc aagaaagatc ggaagaaggt ttgggtttga ggctcagtgg aaggtgagta
     2221 gaagttgata atttgaaagt ggagtagtgt ctatggggtt tttgccttaa atgacagaat
     2281 acattcccaa tataccaaac ataactgttt cctactagtc ggccgtacgg gccctttcgt
     2341 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
     2401 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
     2461 gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg
     2521 caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgg
     2581 ccttaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     2641 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     2701 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     2761 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     2821 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     2881 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     2941 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     3001 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     3061 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     3121 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     3181 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     3241 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     3301 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     3361 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     3421 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     3481 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     3541 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     3601 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     3661 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     3721 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     3781 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     3841 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     3901 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     3961 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     4021 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     4081 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     4141 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     4201 gcattgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     4261 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     4321 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     4381 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     4441 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     4501 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     4561 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     4621 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     4681 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     4741 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     4801 ccatgattac gccaagctgt aagtttaaac atgatcttac taactaacta ttctcattta
     4861 aattttcaga gcttaaaaat ggctgaaatc actcacaacg atggatacgc taacaacttg
     4921 gaaatgaaat
//
