LOCUS       sequence-208668-         712 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Universal mini-vector for efficient cloning of DNA and expression of
            protein in E. coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Liu X, Li T, Hart DJ, Gao S, Wang H, Gao H, Xu S, Zhang Y, Liu Y, An
            Y
  TITLE     A universal mini-vector and an annealing of PCR products (APP)-based
            cloning strategy for convenient molecular biological manipulations.
  JOURNAL   Biochem Biophys Res Commun. 2018 Mar 18;497(4):978-982. doi: 
            10.1016/j.bbrc.2018.02.099. Epub 2018 Feb 12.
  PUBMED    29448102
REFERENCE   2  (bases 1 to 712)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bbrc.2018.02.099"; journalName: "Biochem Biophys Res 
            Commun"; date: "2018-03-18- 18"; volume: "497"; issue: "4"; pages: 
            "978-982"
COMMENT     Sequence Label: pANY1
FEATURES             Location/Qualifiers
     source          1..712
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(44..349)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(146..165)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 cctgacggtc agtcgactgg ctgtgtataa gggagcctga catttatatt ccccagaaca
       61 tcaggttaat ggcgtttttg atgtcatttt cgcggtggct gagatcagcc acttcttccc
      121 cgataacgga gaccggcaca ctggccatat cggtggtcat catgcgccag ctttcatccc
      181 cgatatgcac caccgggtaa agttcacggg agactttatc tgacagcaga cgtgcactgg
      241 ccagggggat caccatccgt cgcccgggcg tgtcaataat atcactctgt acatccacaa
      301 acagacgata acggctctct cttttatagg tgtaaacctt aaactgcatt tcaccagccc
      361 ctgttctcgt cagcaaaaga gccgttcatt tcaataaacc gggcgacctc agccatccct
      421 tcctgatttt ccgctttcca gcgttcggca cgcagacgac gggcttcatt ctgcatggtt
      481 gtgcttacca gaccggagat attgacatca tatatgcctt gagcaactga tagctgtcgc
      541 tgtcaactgt cactgtaata cgctgcttca tagcatacct ctttttgaca tacttcgggt
      601 atacatatca gtatatattc ttataccgca aaaatcagcg cgcaaatacg catactgtta
      661 tctggctttt agtaagccgg actgaccgtc gacaggccta ctagtggatc cc
//
