LOCUS       sequence-208754-       12442 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses pentamerised full-length protein ectodomain with no 
            N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 
            d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12442)
  AUTHORS   Knockel J, Dundas K, Yang ASP, Galaway F, Metcalf T, Gemert GV, 
            Sauerwein RW, Rayner JC, Billker O, Wright GJ
  TITLE     Systematic Identification of Plasmodium Falciparum Sporozoite 
            Membrane Protein Interactions Reveals an Essential Role for the p24 
            Complex in Host Infection.
  JOURNAL   Mol Cell Proteomics. 2021;20:100038. doi: 10.1074/mcp.RA120.002432. 
            Epub 2021 Jan 27.
  PUBMED    33515807
REFERENCE   2  (bases 1 to 12442)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics."; date: "2021"; volume: "20:100038. doi"; pages: " 
            10.1074/mcp.RA120.002432. Epub 2021 Jan 27"
COMMENT     Sequence Label: CCp2-COMP-blac-flag-his
FEATURES             Location/Qualifiers
     source          1..12442
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     310..329
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      352..361
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5974..6759
                     /codon_start=1
                     /gene="bla(M)"
                     /product="beta-lactamase lacking the signal sequence"
                     /label=bla(M)
                     /note="allows cytosolic expression of beta-lactamase"
                     /translation="PETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMST
                     FKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMS
                     DNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATT
                     LRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAA
                     LGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
     CDS             6766..6831
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             6838..6855
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    6893..6948
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6947..6966)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(9184..9202)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9270..9374
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9375..9443,9444..10235)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      10406..10994
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10895..10914
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11148..11165
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11282..11303
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11318..11323,11324..11341,11342..11348)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11356..11372
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11361..11383
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11380..11396
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11380..11396
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        11667..12046
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        12047..12250
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     12201..12221
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     12247..12271
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 ctcgagaaag gcgtctaacc agtcacagtc gcaaggtagg ctgagcaccg tggcgggcgg
       61 cagcgggtgg cggtcggggt tgtttctggc ggaggtgctg ctgatgatgt aattaaagta
      121 ggcggtcttg agacggcgga tggtcgaggt gaggtgtggc aggcttgaga tccagctgtt
      181 ggggtgagta ctccctctca aaagcgggca ttacttctgc gctaagattg tcagtttcca
      241 aaaacgagga ggatttgata ttcacctggc ccgatctggc catacacttg agtgacaatg
      301 acatccactt tgcctttctc tccacaggtg tccactccca ggtccaagtt tgccaccatg
      361 gagtttcaga cccaggtact catgtccctg ctgctctgca tgtctggtgc ggccgccggc
      421 gacgatatca gcaacacctt cttcaagttc gagttctgcg aggccaccag caccttcagc
      481 agcatcggcg agaacggcct gccccagtac gccgccgaga atgccctgac cagaggcagc
      541 ggctactggt gcagcgaggg caagcacaac gtgaacgacg tggtgtcctg gatcggccac
      601 ctgaagaacg tgcggagcct gaacggcatc atcatccact gggcctacac ccctggcgag
      661 gtgtccatcc tggccagcta cgacggcaac gagccctacg aggaagtggt gccctaccag
      721 ctgctggaaa gcagagtggg caacgtggtg cagaacatca tcttcaacca cgtgatccgg
      781 gccaagagca tcaaagtgaa catgcggcac gccatccacg actacttcgg catcaacttc
      841 gccaacgtgc tgggcagccg ggaccccacc ctgagaatcc agagcggcat gagcagcctg
      901 acccaggacc tgtgcctgca gatcgacgag aagaacgagg tggtgctgga cggctgcatc
      961 accgccatca gctacctgga cggccgggac ctgtggaagc tgaacagcaa gaaccagatc
     1021 tacaacccca tcaacaacct gtgcatcacc ctgaaggaca acctgatcgc caacggcggc
     1081 agactgatcc tggaagattg caacgccgcc ctggaacaca acgacggcag aagctcctgg
     1141 cagctgctgc ccaacaacca gctgaagatc ctgcggaacg gcaacttctg tctgagccag
     1201 gacggccaca agagcggcag catcgacgtg gccttccaca aagagtgcac ctccaccctg
     1261 tccagcgaca acaagaacca cgcccctgac aaggtggtgg acggcctgct ggacaccttc
     1321 tgggtgtccc aggaattcaa cctggacacc gccccagaca gcgtgcactt cgacgtgaac
     1381 ctgggcagca tctacaagct gcagaaggcc atcatcgact ggaagtaccc cgccaccaag
     1441 tacagcatca gcctgagcaa cgatggcgag aactacaaag aggtgtcctc caacctggcc
     1501 aacttcctgc ggagcacaat caacaatctg cacaacaccg aggcccagta catccggctg
     1561 accctgatgg cccctaaccc cgagttcagc gaagagaaca agctgttcta cggcatcaag
     1621 aagttcagcg tctacagcaa ccggatcaag tccatcgtgg acgactgcga caagatcaag
     1681 gacaccgacg acgcccggga caagtacttc ttcgagtttg tgtccgaagt gaacctgcag
     1741 gaaggcaaag agctgaagcg gctggacaac gaactgcagc tgtacgccga gaagatccag
     1801 aacgaggccc tgaagattca gtccctgaac cccaagctga agaagtgcaa gctggaaaaa
     1861 gagaagcggc acaaggacat ctccaacatc aagaacgtga tcctcaagaa catctacgaa
     1921 gtgatcaagc agaccgagaa tatcatcaag atgaaccctc tgagcagcta ctacagcacc
     1981 agcacaaaag agctgggcca gacctccgac aaccccgccg acaactgctt ccacctgaaa
     2041 aatgccctgc cctccagccc cagcggcttc tactacgtgc tgaccacctg tagccagaac
     2101 gtgctgcggg tgttctgcga catgaagatg ggcgccacct actacatccc cagcgtggac
     2161 aacaaaatca tcaacaagct gaaagacgtc gagaacgtgt gtgccaccta cggcctgaac
     2221 cctatccacc tgtaccacga gagccagatc tataccctgc ggaaggtgtt cgacaccatg
     2281 gacatcaata tcgccaaccc cgtgcccctg gccatccgga aagaggacag cgagttctac
     2341 tactccctgg acttccagac caacgtgcac gacatcattg ccaagttcgg cacccctgtg
     2401 ggcaatacct tcgggatcaa caacatcggc atcacattct tcgacagcag cagctccgag
     2461 atgagcgcct tcgtgtgcag cgacaatatc aactccatca acctgcccga gcccttcgtg
     2521 aatctggact gccagtcctc cctgaaagag acaaacgaga tcgagaagat gatcgggaac
     2581 gagtacctga tcaagtgccc tcacgactgc ctggaacggg acatcgagga aagcgtgatc
     2641 ggcggcgagg gaaacatcta cagcgaggac agctccatct gcctgagcgc cattcacgcc
     2701 ggcatctacg acaagcacta cctgatccac ctgagagtga tcaacgccct gaatgagtac
     2761 ggcggcttct tccagaacgg aatcatctcc gagagctttt tcaacaacgc ccaggaagtg
     2821 ggcttcaagc tgtttcacgt gccacccaag tgccccaagg acgacatcac cagcaacatc
     2881 aacaataaca acaattacta ctactatgac aacaacaatg ccaacgccat gtttagcttt
     2941 ctcgagctgg ataacaaaat gaacaacgtc aacgataagt tcgacaacaa cgactacacc
     3001 tacgtggact ccagcaccgc cgacgccatc aacgatctga tcaccatcgt gaacaagcag
     3061 gtcggaagca ccgacaccac ctttctggcc ctgattaaca agcagtccat caagatcatc
     3121 agcaacgcca gaagatacct caagcccacc gagatcttcg agaagaatat cgagctgctg
     3181 agcaatgagg ctctgaagga cgtggaaaag gtgttcaatc tgatcaaggt gctcagctcc
     3241 aagatcaaca gcgaactcga gaaaaagaag tataagctcg agatcctggt ggacgagcgg
     3301 ctgcggcaga aagagttcga gagctggaag ctggacaata tcgacaacat ctatgacacc
     3361 ttcgagatca tcaatagcgt gcagctccag cagatcggca agtggaacat cctggacaac
     3421 cctctgtacg agggcatcaa cggcattacc ctgatccaga atgtgcgggt gtacaacagc
     3481 cccgagaaca gcgtgatcaa tagcttcaac ggcgcctacg ccttcctgag atacaagagc
     3541 ttctacgact tcgtgttcag cacctatgtg aacatcaagg gcgtgggcag cgtgggcctg
     3601 atcttcagaa gctacgataa gtacaacttc tacatgctcg agctgaacaa tgaccggcag
     3661 aagaatgagt tcaacaagcg cctgctgaag tttgagaaca atatcgtgac cgagctggcc
     3721 attgtgaacg gcaatgatct ccaggaaggg gattggttcg tcgtccggat cgagtgcatc
     3781 ggcagcaaga tcattatcac cgtgctcaag accaacaagc ctatctacga gctgcccaag
     3841 cccgatatca tcatcaacga cgacttcacc tccagcggca ccatcggctt ttacacatac
     3901 ggcatcgaca acgtgcagtt caccaacatt gccgtggaat ctgtggaatg cagcaccaaa
     3961 gagatcctga gctacaatat cgctcccatc agctgcaata tctatgaaga gtactacgtg
     4021 ggcaagttta acaaggccta catccctttc gacagcgaga actccaacag cggctccagc
     4081 aactggaagt tcgccaagaa cattggcaac gagaagcacg tcatcctgca gaacagcaat
     4141 atgaagcaga ttgagaacga ggaacagatc cccagcttca tcattctcca gaacaaagcc
     4201 tgccagaccg gcgtgctgaa tttcgccgtg taccccgagt gctccaacgg catcgtgggc
     4261 accatgttca agtttctgga ctccaagaac tacgccatcc tggaaatcgg ctccggcttc
     4321 acccggctga gacagaatgt gaatggcaag tttcagctgc tgtctaagtc catcatcagc
     4381 gggtacaaag agcacatctg gaacagagtg accgtgtcct tctcctccaa caacattaac
     4441 gtcaacctgg gaaccggctt catgacctac cccatcttct ccctgatcgg cctgcacctg
     4501 agcgacggcg agagcgtggg ctttaccagc tacaactgcg ccaatgtggc cttctccaat
     4561 atctacatgc accccttcga cttcaagccc tacacaccca cccctaccct ggataccgag
     4621 tccttcctgc ctcctatctt cagcaagttc gaccaggcca ccatcaaaga agaggaccag
     4681 tcccaggaca tgggctacaa acagatcggc gataacaaga atagcgacat cagcaaggac
     4741 agccctatcg ataagcacag cttcgaggac tccacccggc agatgaagaa ggacgcctac
     4801 tattgcgcca cccacaaaaa catcgtggat atcattaact actgcaacca gtacgacaaa
     4861 gagaacgaca attgcgccaa cgagttctgt accatctgct gcaacaatat tgataccaaa
     4921 gaggaagagg acatccggac ctgcgagatt ctgtgccaga aactggacga caagattctg
     4981 cagaccagcg aagtgctgaa ctacctcaag aagtcctgca tcgagagccc caatgaggaa
     5041 ctgaagaaaa gctgcgagga cgacaatgac aaagaagaat gcctcatcga gatgtgcgaa
     5101 atgtgctgcc agagcgtgac catccccgac gacctgctga ccagccacat ggatatcgac
     5161 tccctgacca accactgcat ctccctgtgc gacaagcccg gcgcgccgtc gacctccatc
     5221 acggcctata agagtgaggg ggagtcagcg gagttctcct tcccactcaa ccttggagag
     5281 gaaagcctgc agggagagtt gagatggaag gcagagaagg ctccttcttc ccagtcctgg
     5341 atcaccttct ccctaaagaa ccaaaaggtg tctgtgcaga agtctactag caaccccaag
     5401 ttccagctgt ccgaaacgct cccactcacc cttcagatac cccaggtctc ccttcagttt
     5461 gctggttctg gcaacctgac cctgactctg gacagaggga tactgtatca ggaagtgaac
     5521 ctggtggtga tgaaagtgac tcagcccgac agcaacactt tgacctgtga ggtgatggga
     5581 cccacctcac ccaagatgag actgatcttg aagcaggaga atcaggaggc cagggtctcc
     5641 aggcaggaga aagtgattca agtgcaggcc cctgaagcag gggtgtggca atgtctactg
     5701 agtgaaggtg aagaggtcaa gatggactcc aagatccagg ttttatccaa agggttgaat
     5761 tccggcggcg gctccggagg cggtaccggt ggcggcgacc tagccccaca gatgcttcga
     5821 gaactccagg agactaatgc ggcgctgcaa gacgtgagag agctcttgcg acagcaggtc
     5881 aaggagatca ccttcctgaa gaatacggtg atggaatgtg acgcctgcgg tctagacaga
     5941 aacctgcctc cactagcacc tctgggccca cacccagaaa cgctggtgaa agtaaaagat
     6001 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     6061 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     6121 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     6181 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     6241 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     6301 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     6361 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     6421 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     6481 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     6541 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     6601 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     6661 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     6721 cagatcgctg agataggtgc ctcactgatt aagcattggg gccctgacta caaagaccat
     6781 gacggtgatt ataaagatca tgacatcgat tacaaggatg acgatgacaa gggccctcac
     6841 caccaccatc accactaatt aattaaggat cccccgacct cgacctctgg ctaataaagg
     6901 aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca
     6961 tatgggaggg caaatcattt ggtcgagatc cctcggagat ctctagctag aggatcgatc
     7021 cccgccccgg acgaactaaa cctgactacg acatctctgc cccttcttcg cggggcagtg
     7081 catgtaatcc cttcagttgg ttggtacaac ttgccaactg ggccctgttc cacatgtgac
     7141 acgggggggg accaaacaca aaggggttct ctgactgtag ttgacatcct tataaatgga
     7201 tgtgcacatt tgccaacact gagtggcttt catcctggag cagactttgc agtctgtgga
     7261 ctgcaacaca acattgcctt tatgtgtaac tcttggctga agctcttaca ccaatgctgg
     7321 gggacatgta cctcccaggg gcccaggaag actacgggag gctacaccaa cgtcaatcag
     7381 aggggcctgt gtagctaccg ataagcggac cctcaagagg gcattagcaa tagtgtttat
     7441 aaggccccct tgttaaccct aaacgggtag catatgcttc ccgggtagta gtatatacta
     7501 tccagactaa ccctaattca atagcatatg ttacccaacg ggaagcatat gctatcgaat
     7561 tagggttagt aaaagggtcc taaggaacag cgatatctcc caccccatga gctgtcacgg
     7621 ttttatttac atggggtcag gattccacga gggtagtgaa ccattttagt cacaagggca
     7681 gtggctgaag atcaaggagc gggcagtgaa ctctcctgaa tcttcgcctg cttcttcatt
     7741 ctccttcgtt tagctaatag aataactgct gagttgtgaa cagtaaggtg tatgtgaggt
     7801 gctcgaaaac aaggtttcag gtgacgcccc cagaataaaa tttggacggg gggttcagtg
     7861 gtggcattgt gctatgacac caatataacc ctcacaaacc ccttgggcaa taaatactag
     7921 tgtaggaatg aaacattctg aatatcttta acaatagaaa tccatggggt ggggacaagc
     7981 cgtaaagact ggatgtccat ctcacacgaa tttatggcta tgggcaacac ataatcctag
     8041 tgcaatatga tactggggtt attaagatgt gtcccaggca gggaccaaga caggtgaacc
     8101 atgttgttac actctatttg taacaagggg aaagagagtg gacgccgaca gcagcggact
     8161 ccactggttg tctctaacac ccccgaaaat taaacggggc tccacgccaa tggggcccat
     8221 aaacaaagac aagtggccac tctttttttt gaaattgtgg agtgggggca cgcgtcagcc
     8281 cccacacgcc gccctgcggt tttggactgt aaaataaggg tgtaataact tggctgattg
     8341 taaccccgct aaccactgcg gtcaaaccac ttgcccacaa aaccactaat ggcaccccgg
     8401 ggaatacctg cataagtagg tgggcgggcc aagatagggg cgcgattgct gcgatctgga
     8461 ggacaaatta cacacacttg cgcctgagcg ccaagcacag ggttgttggt cctcatattc
     8521 acgaggtcgc tgagagcacg gtgggctaat gttgccatgg gtagcatata ctacccaaat
     8581 atctggatag catatgctat cctaatctat atctgggtag cataggctat cctaatctat
     8641 atctgggtag catatgctat cctaatctat atctgggtag tatatgctat cctaatttat
     8701 atctgggtag cataggctat cctaatctat atctgggtag catatgctat cctaatctat
     8761 atctgggtag tatatgctat cctaatctgt atccgggtag catatgctat cctaatagag
     8821 attagggtag tatatgctat cctaatttat atctgggtag catatactac ccaaatatct
     8881 ggatagcata tgctatccta atctatatct gggtagcata tgctatccta atctatatct
     8941 gggtagcata ggctatccta atctatatct gggtagcata tgctatccta atctatatct
     9001 gggtagtata tgctatccta atttatatct gggtagcata ggctatccta atctatatct
     9061 gggtagcata tgctatccta atctatatct gggtagtata tgctatccta atctgtatcc
     9121 gggtagcata tgctatcctc atgataagct gtcaaacatg agaattttct tgaagacgaa
     9181 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     9241 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     9301 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     9361 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     9421 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     9481 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     9541 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     9601 gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt
     9661 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     9721 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     9781 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     9841 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     9901 gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac
     9961 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
    10021 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
    10081 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
    10141 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
    10201 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
    10261 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
    10321 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
    10381 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
    10441 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
    10501 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
    10561 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
    10621 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
    10681 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
    10741 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
    10801 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
    10861 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
    10921 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
    10981 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
    11041 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
    11101 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
    11161 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
    11221 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
    11281 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
    11341 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
    11401 attacgccaa gctctagcta gaggtcgacc aattctcatg tttgacagct tatcatcgca
    11461 gatccgggca acgttgttgc cattgctgca ggcgcagaac tggtaggtat ggaagatcta
    11521 tacattgaat caatattggc aattagccat attagtcatt ggttatatag cataaatcaa
    11581 tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg
    11641 ctcatgtcca atatgaccgc catgttgaca ttgattattg actagttatt aatagtaatc
    11701 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
    11761 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
    11821 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
    11881 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtccgc cccctattga
    11941 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tacgggactt
    12001 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
    12061 gcagtacacc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
    12121 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
    12181 taataacccc gccccgttga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
    12241 aagcagagct cgtttagtga accgtcagat cctcactctc ttccgcatcg ctgtctgcga
    12301 gggccagctg ttgggctcgc ggttgaggac aaactcttcg cggtctttcc agtactcttg
    12361 gatcggaaac ccgtcggcct ccgaacggta ctccgccacc gagggacctg agcgagtccg
    12421 catcgaccgg atcggaaaac ct
//
