LOCUS       sequence-208759-       10549 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses pentamerised full-length protein ectodomain with no 
            N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 
            d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10549)
  AUTHORS   Knockel J, Dundas K, Yang ASP, Galaway F, Metcalf T, Gemert GV, 
            Sauerwein RW, Rayner JC, Billker O, Wright GJ
  TITLE     Systematic Identification of Plasmodium Falciparum Sporozoite 
            Membrane Protein Interactions Reveals an Essential Role for the p24 
            Complex in Host Infection.
  JOURNAL   Mol Cell Proteomics. 2021;20:100038. doi: 10.1074/mcp.RA120.002432. 
            Epub 2021 Jan 27.
  PUBMED    33515807
REFERENCE   2  (bases 1 to 10549)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics."; date: "2021"; volume: "20:100038. doi"; pages: " 
            10.1074/mcp.RA120.002432. Epub 2021 Jan 27"
COMMENT     Sequence Label: SIAP-1-COMP-blac-flag-his
FEATURES             Location/Qualifiers
     source          1..10549
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     310..329
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      352..361
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4081..4866
                     /codon_start=1
                     /gene="bla(M)"
                     /product="beta-lactamase lacking the signal sequence"
                     /label=bla(M)
                     /note="allows cytosolic expression of beta-lactamase"
                     /translation="PETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMST
                     FKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMS
                     DNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATT
                     LRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAA
                     LGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
     CDS             4873..4938
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             4945..4962
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    5000..5055
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5054..5073)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7291..7309)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7377..7481
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7482..7550,7551..8342)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8513..9101
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9002..9021
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9255..9272
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9389..9410
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9425..9430,9431..9448,9449..9455)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9463..9479
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9468..9490
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9487..9503
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9487..9503
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        9774..10153
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10154..10357
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10308..10328
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     10354..10378
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 ctcgagaaag gcgtctaacc agtcacagtc gcaaggtagg ctgagcaccg tggcgggcgg
       61 cagcgggtgg cggtcggggt tgtttctggc ggaggtgctg ctgatgatgt aattaaagta
      121 ggcggtcttg agacggcgga tggtcgaggt gaggtgtggc aggcttgaga tccagctgtt
      181 ggggtgagta ctccctctca aaagcgggca ttacttctgc gctaagattg tcagtttcca
      241 aaaacgagga ggatttgata ttcacctggc ccgatctggc catacacttg agtgacaatg
      301 acatccactt tgcctttctc tccacaggtg tccactccca ggtccaagtt tgccaccatg
      361 gagtttcaga cccaggtact catgtccctg ctgctctgca tgtctggtgc ggccgcctac
      421 aacatcaaga gcggcaacac cgccaacaat atcaaatacg tgaacgtgct ggacaacgac
      481 cgggacatca acacccacag cgtgctgccc gaggtggaaa acgtgatcga gcggaaggac
      541 atctaccggc agatcaactt catggaaacc ttcgtgtcca gcaacaacat gatgcacgac
      601 agagagaagc acaccagcaa cgacgccggc agctacgaga tcaccggcat cgtggacggc
      661 atgaagatcg gccaccccat cagcgtggcc ctgggcagcc agtacagcaa ctacttcgac
      721 tacctgcaga tcgtgcacct ggactacacc aacagccggt tcagcttcac cgtgggcgag
      781 ggcaagtact acctgcggac ctacggcagc acctacatga cccctagcgc catcaagatc
      841 aaggtgccct gcgagaagtg caagttcatc aacagcgagt acagcggcat catcaagatt
      901 atcccttacg agacaaacaa caacctgttc atctacaact gggtgctgca gaccagcagc
      961 cctctggccc tggaaaacat caataccgtg ttcagcgacg aggccgacct gatccacggc
     1021 aacagcctga gcgaggaatt caagatcgac agcagcgccg ctgccaccag cctgaacacc
     1081 ttctatggca tcgtgctgca cggcatctgg tcctccgagt acgccgagcg gctgctgacc
     1141 gtgatctccg agttccccga ctgcgtgaag atgagcgccc acgacaagaa cgccagaagc
     1201 aagcagcgga agaaccagaa gtggatcctg gtcaacgagg acctgggctc cttcgacatg
     1261 aagatggaag tgtgcgagga agtgaactgc gactactccg ccatcatcca cgtgtccaag
     1321 cacgccttcg agtactccaa gaaactggtg cacaaccggg gcagaaacgg ccggtactac
     1381 agcagacggg tggaaaagat cctgatccgg gccctgctga gcctggactt cagcctgttt
     1441 atcacctact tccagcagaa acacggcgtg accctgctgg accctcagta cgactacgag
     1501 ctgatcacca acatggccgg ctacagctcc aacaactacc agagctggaa ccacaacctg
     1561 gaagaactgg tggaactggc caccagctgg gacgagtacc ccaagggcct gcagaaggtg
     1621 cagggcctga gctacctgct gcggagaaag aacggcgcca agcaccccgt gtaccccaca
     1681 gcccctgccg tggcctttcc agccggcagc cagaacaacg ccttcatcga gttcatggaa
     1741 tccgccttcg tgaactacgt ggacatcagc cacctggtca tccacgaggt ggcccacttc
     1801 atctgggtca acaccgtgtc caaagaactg aaagagaaat ggatccagat cgggcagtgg
     1861 tacaaagagc ccctgagccc cagcgaatgg gccacaaagc tggaagtgga attcgtgtcc
     1921 gcctacgccc atgataagaa ccccgccgag gacttcgccg agagcatggc cacctacgtg
     1981 ctgaacagca agctgctgaa ctccagaagc ttcgacaagt tcaagtggat tcaggacaat
     2041 ctgttcggcg gaggcttcta catcaccacc ggcacccaca agttcgacgt gatcaacctg
     2101 ggcaatgagg tgtactactt ccccggcaaa gtgaccagag tgcgggccaa ggtgctgggc
     2161 tcccctaccg aggacaagct ggtcaagatc tacatcagcc tgctgtccag cgacggcagc
     2221 gagggctgtg ccaagcacgg ctacgcccgg atcttcagcg agcagcagac cttccgggac
     2281 ctgtacttcc acaccgagga tagaagcccc tgcagccaca agctgtacgg cgagttcacc
     2341 atgaacaagc acgagagcag aggccggtgg accgccgagt ccatgatctt caccggcgag
     2401 aacaacatcg agagatacgt gggcctgggc tctttccact tctacctgta cgtgaacaac
     2461 cagaacgagg acgtggaaaa gcctatccct ctgctggact ccatcagcat ctacacacac
     2521 aacgccgccg agactaacga cgctctgctg cggctgcacg tgatggtgct ggaaaatgaa
     2581 ctgatcaaag agcacggcgg accttacgcc agcttcgccg cccacgagaa caaggcctac
     2641 tcctacgaga gccggaccta caagatgtac cctcccgagt tcaacaccct gatgctgaag
     2701 gccgactact tcatcagaga tatcaacacc agaggcttcc gcgaagtgaa catggacagc
     2761 tgcaagagct acaccaatat ggacacccgg aacctgaagt gcttccaggt gctgaacccc
     2821 gtgaccatcc ccaagtactg tatcggctcc acctactttc tgagacaggt gtccatcgag
     2881 gatatcgccg gcaatctgga aaccgtgaat atcgccagcg acaagtacag cgccagactg
     2941 caccctatcg gcgtgcggga caagcagaaa cccgtggtgt ccaacgtgcg ggtgtcctcc
     3001 aagcccgcca acgagtacca cgacggcgag acaatcgtgt ccctgagctt caacgtgcac
     3061 gacaacctgg ccggcgtgta ctacatcttc gtgtacctgc gggatcccca cggcggaaag
     3121 cacagaagcg acatcgaccg ggccagcctg cctaccggca cagagaacaa gcagatcaat
     3181 cacaagatcc tgctgcccaa gggctccatg ggcggcacct ggatgctgga agagatcaag
     3241 gccgtggact cctgcaagaa cgaggcccgg aatatctaca cccactccgt gtacgtgcag
     3301 aacgacggcg cgccgtcgac ctccatcacg gcctataaga gtgaggggga gtcagcggag
     3361 ttctccttcc cactcaacct tggagaggaa agcctgcagg gagagttgag atggaaggca
     3421 gagaaggctc cttcttccca gtcctggatc accttctccc taaagaacca aaaggtgtct
     3481 gtgcagaagt ctactagcaa ccccaagttc cagctgtccg aaacgctccc actcaccctt
     3541 cagatacccc aggtctccct tcagtttgct ggttctggca acctgaccct gactctggac
     3601 agagggatac tgtatcagga agtgaacctg gtggtgatga aagtgactca gcccgacagc
     3661 aacactttga cctgtgaggt gatgggaccc acctcaccca agatgagact gatcttgaag
     3721 caggagaatc aggaggccag ggtctccagg caggagaaag tgattcaagt gcaggcccct
     3781 gaagcagggg tgtggcaatg tctactgagt gaaggtgaag aggtcaagat ggactccaag
     3841 atccaggttt tatccaaagg gttgaattcc ggcggcggct ccggaggcgg taccggtggc
     3901 ggcgacctag ccccacagat gcttcgagaa ctccaggaga ctaatgcggc gctgcaagac
     3961 gtgagagagc tcttgcgaca gcaggtcaag gagatcacct tcctgaagaa tacggtgatg
     4021 gaatgtgacg cctgcggtct agacagaaac ctgcctccac tagcacctct gggcccacac
     4081 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     4141 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     4201 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     4261 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     4321 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     4381 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     4441 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     4501 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     4561 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     4621 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     4681 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     4741 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     4801 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     4861 cattggggcc ctgactacaa agaccatgac ggtgattata aagatcatga catcgattac
     4921 aaggatgacg atgacaaggg ccctcaccac caccatcacc actaattaat taaggatccc
     4981 ccgacctcga cctctggcta ataaaggaaa tttattttca ttgcaatagt gtgttggaat
     5041 tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttggt cgagatccct
     5101 cggagatctc tagctagagg atcgatcccc gccccggacg aactaaacct gactacgaca
     5161 tctctgcccc ttcttcgcgg ggcagtgcat gtaatccctt cagttggttg gtacaacttg
     5221 ccaactgggc cctgttccac atgtgacacg gggggggacc aaacacaaag gggttctctg
     5281 actgtagttg acatccttat aaatggatgt gcacatttgc caacactgag tggctttcat
     5341 cctggagcag actttgcagt ctgtggactg caacacaaca ttgcctttat gtgtaactct
     5401 tggctgaagc tcttacacca atgctggggg acatgtacct cccaggggcc caggaagact
     5461 acgggaggct acaccaacgt caatcagagg ggcctgtgta gctaccgata agcggaccct
     5521 caagagggca ttagcaatag tgtttataag gcccccttgt taaccctaaa cgggtagcat
     5581 atgcttcccg ggtagtagta tatactatcc agactaaccc taattcaata gcatatgtta
     5641 cccaacggga agcatatgct atcgaattag ggttagtaaa agggtcctaa ggaacagcga
     5701 tatctcccac cccatgagct gtcacggttt tatttacatg gggtcaggat tccacgaggg
     5761 tagtgaacca ttttagtcac aagggcagtg gctgaagatc aaggagcggg cagtgaactc
     5821 tcctgaatct tcgcctgctt cttcattctc cttcgtttag ctaatagaat aactgctgag
     5881 ttgtgaacag taaggtgtat gtgaggtgct cgaaaacaag gtttcaggtg acgcccccag
     5941 aataaaattt ggacgggggg ttcagtggtg gcattgtgct atgacaccaa tataaccctc
     6001 acaaacccct tgggcaataa atactagtgt aggaatgaaa cattctgaat atctttaaca
     6061 atagaaatcc atggggtggg gacaagccgt aaagactgga tgtccatctc acacgaattt
     6121 atggctatgg gcaacacata atcctagtgc aatatgatac tggggttatt aagatgtgtc
     6181 ccaggcaggg accaagacag gtgaaccatg ttgttacact ctatttgtaa caaggggaaa
     6241 gagagtggac gccgacagca gcggactcca ctggttgtct ctaacacccc cgaaaattaa
     6301 acggggctcc acgccaatgg ggcccataaa caaagacaag tggccactct tttttttgaa
     6361 attgtggagt gggggcacgc gtcagccccc acacgccgcc ctgcggtttt ggactgtaaa
     6421 ataagggtgt aataacttgg ctgattgtaa ccccgctaac cactgcggtc aaaccacttg
     6481 cccacaaaac cactaatggc accccgggga atacctgcat aagtaggtgg gcgggccaag
     6541 ataggggcgc gattgctgcg atctggagga caaattacac acacttgcgc ctgagcgcca
     6601 agcacagggt tgttggtcct catattcacg aggtcgctga gagcacggtg ggctaatgtt
     6661 gccatgggta gcatatacta cccaaatatc tggatagcat atgctatcct aatctatatc
     6721 tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc
     6781 tgggtagtat atgctatcct aatttatatc tgggtagcat aggctatcct aatctatatc
     6841 tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct aatctgtatc
     6901 cgggtagcat atgctatcct aatagagatt agggtagtat atgctatcct aatttatatc
     6961 tgggtagcat atactaccca aatatctgga tagcatatgc tatcctaatc tatatctggg
     7021 tagcatatgc tatcctaatc tatatctggg tagcataggc tatcctaatc tatatctggg
     7081 tagcatatgc tatcctaatc tatatctggg tagtatatgc tatcctaatt tatatctggg
     7141 tagcataggc tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg
     7201 tagtatatgc tatcctaatc tgtatccggg tagcatatgc tatcctcatg ataagctgtc
     7261 aaacatgaga attttcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta
     7321 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     7381 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     7441 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     7501 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     7561 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     7621 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     7681 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag
     7741 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     7801 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     7861 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     7921 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     7981 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa
     8041 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     8101 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     8161 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     8221 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     8281 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     8341 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     8401 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     8461 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     8521 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     8581 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     8641 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     8701 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     8761 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     8821 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     8881 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     8941 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     9001 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     9061 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     9121 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     9181 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     9241 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     9301 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     9361 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     9421 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     9481 ttcacacagg aaacagctat gaccatgatt acgccaagct ctagctagag gtcgaccaat
     9541 tctcatgttt gacagcttat catcgcagat ccgggcaacg ttgttgccat tgctgcaggc
     9601 gcagaactgg taggtatgga agatctatac attgaatcaa tattggcaat tagccatatt
     9661 agtcattggt tatatagcat aaatcaatat tggctattgg ccattgcata cgttgtatct
     9721 atatcataat atgtacattt atattggctc atgtccaata tgaccgccat gttgacattg
     9781 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
     9841 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     9901 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     9961 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
    10021 tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
    10081 tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg tattagtcat
    10141 cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat agcggtttga
    10201 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
    10261 aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg
    10321 taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcct
    10381 cactctcttc cgcatcgctg tctgcgaggg ccagctgttg ggctcgcggt tgaggacaaa
    10441 ctcttcgcgg tctttccagt actcttggat cggaaacccg tcggcctccg aacggtactc
    10501 cgccaccgag ggacctgagc gagtccgcat cgaccggatc ggaaaacct
//
