LOCUS       sequence-208774-        8655 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  inducible expression of vsv-INCENP d543-746iPRC1 279-482-EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8655)
  AUTHORS   van der Horst A, Vromans MJ, Bouwman K, van der Waal MS, Hadders MA,
            Lens SM
  TITLE     Inter-domain Cooperation in INCENP Promotes Aurora B Relocation from
            Centromeres to Microtubules.
  JOURNAL   Cell Rep. 2015 Jul 21;12(3):380-7. doi: 
            10.1016/j.celrep.2015.06.038. Epub 2015 Jul 9.
  PUBMED    26166576
REFERENCE   2  (bases 1 to 8655)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.06.038"; journalName: "Cell Rep"; date: 
            "2015-07-21- 21"; volume: "12"; issue: "3"; pages: "380-7"
COMMENT     Sequence Label: pcDNA4-Tet-on-vsv-Incenp d543-746iPRC1_279-482...
FEATURES             Location/Qualifiers
     source          1..8655
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             1030..1062
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     regulatory      3892..3901
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3898..3900,3901..3903,3904..4617)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3898..3900,3901..3903,3904..4614)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3943..3964)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4204..4223)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4551..4572
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4663..4680)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4669..4893
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4939..5367
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5026..5045)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5236..5257
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5376..5396)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5381..5710
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5561..5696
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5623..5642
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5758..5805
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5824..6198
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6328..6449
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6365..6384)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6419..6438
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6498..6514)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6498..6514)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6511..6533)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6522..6538
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6546..6552,6553..6570,6571..6576))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6591..6612
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6729..6746)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6900..7488)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6980..6999)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7659..8450,8451..8519))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8282..8301
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8520..8624)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttggtaccg agctcggatc tcgagctcaa gcttggtacc
     1021 gccaccatgt acaccgacat cgagatgaac cggttgggca aggggacgac ggccccaggg
     1081 cccattcacc tgctggagct ctgtgaccag aagctcatgg agtttctctg caacatggat
     1141 aataaggact tggtgtggct tgaggagatc caagaggagg ccgagcgcat gttcaccaga
     1201 gaattcagca aagagccaga gctgatgccc aaaacacctt ctcagaagaa ccgacggaag
     1261 aagagacgga tttcttatgt tcaggatgaa aacagagatc ccatcaggag aaggttatcc
     1321 cgcagaaagt ctcggagcag ccagctgagc tcccgacgcc tccgcagcaa ggacagtgta
     1381 gagaagctgg ctacagtggt cggggagaac ggctccgttc tgcggcgtgt gacccgtgct
     1441 gcggctgcag ctgccgcggc taccatggca ttggctgcac cttcttcacc cacccctgag
     1501 tctcccacga tgctgactaa gaagcccgag gataaccaca cccagtgcca gctggtgcct
     1561 gtggtggaga tcggcatcag tgagcgccag aatgctgagc agcatgtcac ccagctcatg
     1621 tccaccgagc ctctgccccg cactctgtcc ccgactccag cttcagccac agctccaacc
     1681 tcccagggca tcccgacatc agatgaggaa tcaacaccta agaagtcgaa ggccaggata
     1741 ctggagtcca tcacagtgag ctccctgatg gctacacccc aggaccccaa gggtcaaggg
     1801 gtcgggacgg ggcggtctgc gtctaagctc aggattgcgc aggtctcccc tggcccacgg
     1861 gactcgccag cctttccaga ttctccatgg cgggagcggg tgctggctcc catcctgccg
     1921 gataacttct ccacgcccac gggctctcgc acggactctc aatcggtgcg gcacagcccg
     1981 atcgccccgt cttccccgag tccccgagtc ttagcccaga agtactctct ggtggccaaa
     2041 caggaaagtg ttgtccgcag ggcgagcaga aggcttgcca agaagactgc cgaagagcca
     2101 gctgcctctg gccgcatcat ctgtcacagt tacctggaga ggctcctgaa tgttgaggtg
     2161 ccccagaaag ttggttctga gcagaaggaa ccccccgagg aggctgagcc tgtggcggca
     2221 gctgagccag aggtccctga gaacaacgga aataactcgt ggccccacaa tgacacagag
     2281 atcgccaaca gcacacccaa cccgaagcct gcagccagca gcccggaaac accctctgca
     2341 gggcagcaag aggccaagac ggaccaagca gatggaccca gagagccacc gcagagtgcc
     2401 aggaggaagc gcagctacaa gcaggccgtg agtgagctgg acgaggagca gcacctggag
     2461 gatgaggagc tgcagccccc caggagcaag accccttcct caccctgccc agccagcaag
     2521 gtggtacggc ccctccggac ctttctgcac acagtgcaga ggaaccagat gctcacgacc
     2581 ccgacctcag ccccacgcag cgtcatgaag tcctttatta agcgcaacac tcccctgcgc
     2641 atggacccca agtgcagctt cgtcgagaag gagcggcagc gcctggagct taaggaaagc
     2701 agcgtggatc ggttggaaga actgaaaatg caaaacatga agaaagtgat tgaggcaatt
     2761 cgagtggagc tggttcagta ctgggaccag tgcttttata gccaggagca gagacaagct
     2821 tttgcccctt tctgtgctga ggactacaca gaaagtctgc tccagctcca cgatgctgag
     2881 attgtgcggt taaaaaacta ctatgaagtt cacaaggaac tctttgaagg tgtccagaag
     2941 tgggaagaaa cctggaggct tttcttagag tttgagagaa aagcttcaga tccaaatcga
     3001 tttacaaacc gaggaggaaa tcttctaaaa gaagaaaaac aacgagccaa gctccagaaa
     3061 atgctgccca agctggaaga agagttgaag gcacgaattg aattgtggga acaggaacat
     3121 tcaaaggcat ttatggtgaa tgggcagaaa ttcatggagt atgtggcaga acaatgggag
     3181 atgcatcgat tggagaaaga gagagccaag caggaaagac aactgaagaa caaaaaacag
     3241 acagagacag agatgctgta tggcagcgct cctcgaacac ctagcaagcg gcgaggactg
     3301 gctcccaata caccgggaag cagccttaag gagcagctgc agagggaact ggaggagaag
     3361 aagaagaagg aagagcagca gcgtctggct gagcggcagc tgcaggagga gcaagagaag
     3421 aaagccaagg aggcagcagg ggccagcaag gccctgaatg tgactgtgga cgtgcagtct
     3481 ccagcttgta cctcatatca gatgactccg caagggcaca gggcccctcc caagatcaac
     3541 ccagataact acgggatgga tctgaatagc gacgactcca ccgatgatga ggcccatccc
     3601 cggaagccca tccccacctg ggcccgaggc accccgctca gccaggctat cattcaccag
     3661 tactaccacc caccgaacct tctggagctc tttggaacca ttctcccact ggacttggag
     3721 gatatcttca agaagagcaa gccccgctat cacaagcgca ccagctctgc tgtctggaac
     3781 tcaccgcccc tgcagggcgc cagggtcccc agcagcctgg cctacagcct gaagaagcac
     3841 aagcttcgaa ttctgcagtc gacggtaccg cgggcccggg atccaccggt cgccaccatg
     3901 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     3961 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
     4021 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     4081 gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag
     4141 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
     4201 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     4261 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     4321 ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc
     4381 atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
     4441 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     4501 ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     4561 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaaagc
     4621 ggccgctcga gtctagaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta
     4681 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
     4741 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     4801 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
     4861 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg
     4921 gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     4981 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     5041 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     5101 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     5161 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     5221 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     5281 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     5341 tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg
     5401 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     5461 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     5521 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     5581 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     5641 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     5701 cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc agcacgtgtt
     5761 gacaattaat catcggcata gtatatcggc atagtataat acgacaaggt gaggaactaa
     5821 accatggcca agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg
     5881 gtcgagttct ggaccgaccg gctcgggttc tcccgggact tcgtggagga cgacttcgcc
     5941 ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg tccaggacca ggtggtgccg
     6001 gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg
     6061 gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg ccatgaccga gatcggcgag
     6121 cagccgtggg ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg
     6181 gccgaggagc aggactgaca cgtgctacga gatttcgatt ccaccgccgc cttctatgaa
     6241 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
     6301 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa
     6361 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     6421 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag
     6481 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     6541 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     6601 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     6661 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     6721 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     6781 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     6841 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     6901 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     6961 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     7021 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     7081 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     7141 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     7201 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     7261 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     7321 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     7381 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     7441 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     7501 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     7561 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     7621 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     7681 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     7741 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     7801 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     7861 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     7921 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     7981 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     8041 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     8101 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     8161 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     8221 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     8281 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     8341 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     8401 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     8461 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     8521 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     8581 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     8641 gtgccacctg acgtc
//
