LOCUS       sequence-208963-         885 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial Expression of RNF144A RBR domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Michel MA, Swatek KN, Hospenthal MK, Komander D
  TITLE     Ubiquitin Linkage-Specific Affimers Reveal Insights into K6-Linked 
            Ubiquitin Signaling.
  JOURNAL   Mol Cell. 2017 Oct 5;68(1):233-246.e5. doi: 
            10.1016/j.molcel.2017.08.020. Epub 2017 Sep 21.
  PUBMED    28943312
REFERENCE   2  (bases 1 to 885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2017.08.020"; journalName: "Mol Cell"; date: 
            "2017-10-5- 5"; volume: "68"; issue: "1"; pages: "233-246.e5"
COMMENT     Sequence Label: Human RNF144A (16-228)
FEATURES             Location/Qualifiers
     source          1..885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..52)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(715..738)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(777..799)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 cttcctttcg ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgtttaaatg
       61 gtctagaaag ctttaatcgt agtgtatcag aaggaaatca tcgtccagag actccaggca
      121 gtaccagcag aaggcgtgct tgcagttctt gcacatcatc tgcgcgcagc cttcgtctcg
      181 ctcgatgtag actttgcact tggggcagcg cttgatgggc gcgtcatctt cttccatttt
      241 gaaagcagca ctggtctccc cggggaggaa ggtgatcggc atggtctccg ggcagccctg
      301 gccagggtgc cagctggctt tgcaggtgga gcagaattcc atacggcagg ctttgcactg
      361 cactggctgg ggggtctgca gccccacgtc ctggagctga cacacagctt ggcaggtgga
      421 cgccgggcac caagtccgac agggatcaaa cagcacctct ctttcaaatt gtagcttttt
      481 atatctttgc ataatttcag ctgcaaccat gcactcaatc tcgttctcct gtaggtggcc
      541 ctgtttaggg caggcagcat ctgggcagct aattgcggtt tctaatcctt ctttgatcaa
      601 gagctcaaca tactgtttca ggcacagagt acagaagatg cattggcact gggctatggt
      661 tgtcatctgc tccactgggt actccccaag acagagcttg caagacacca gcggcgggcc
      721 ctgaaacaga acttccagac cgctgctcag atccgatttt ggaggatggt cgccaccacc
      781 aaacgtggct tgccagccct gcaaaggcca tgctatatac ttgctggatt tcaagtactt
      841 atcaatttgt gggatagctt caatacgttt tttaaaacaa actaa
//
