LOCUS       sequence-208968-        1054 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial Expression of HUWE1 HECT domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Michel MA, Swatek KN, Hospenthal MK, Komander D
  TITLE     Ubiquitin Linkage-Specific Affimers Reveal Insights into K6-Linked 
            Ubiquitin Signaling.
  JOURNAL   Mol Cell. 2017 Oct 5;68(1):233-246.e5. doi: 
            10.1016/j.molcel.2017.08.020. Epub 2017 Sep 21.
  PUBMED    28943312
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2017.08.020"; journalName: "Mol Cell"; date: 
            "2017-10-5- 5"; volume: "68"; issue: "1"; pages: "233-246.e5"
COMMENT     Sequence Label: Human HUWE1 (3993-4374)
FEATURES             Location/Qualifiers
     source          1..1054
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(55..72)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gggggggcaa gccacttcag cttcctttcg ggctttgtta gcagccggat ctcagtggtg
       61 gtggtggtgg tgtttaaatg gtctagaaag ctttaggcca gcccaaagcc ttcagagcac
      121 tcctggatag ccaacagtag catgtggcgg agcttctcaa agctctcata ggcaggcaga
      181 tccagctgat taaagcatgt gtgagctgaa ggcaggcgat ctgtggacct gtcatctcga
      241 tggatctgaa acttctgaat gccattcatg ccttcgaggg cagcaaagcc ttgcaggggt
      301 accttggaag tacccgtgac aaactggagg aacttggcac ggtcagcttg atcgaaagaa
      361 cgcaatgctc tccagaacca ctggatctga atagagttgg actggtactt gtggtattca
      421 gtgttggatt tcagatcatc gatgtcaatg gtgggcagtc ctgatataag cagctctaac
      481 tcctgctcag tgaagatgga aatgaggcgc tttggaatga tctcatagaa gccttctaag
      541 aaagccgcca actgcttgcg gatggctcct gtcattctca tctggcatac caggtgtaca
      601 tactccttct tattctcctc tgttaccaag atgttggccc cattgggttt gaggtcacga
      661 acttcacaaa ctccaaactc ttggacctca gtgctgaagg tgaggtcata gcctagtgtg
      721 gagacatcat tttccagcag ataaaccaga ccttggtaga agtggtaatc ttcactctcc
      781 atatctgtat atctgactga cttgcccaag atgtgtttgt aaaaggatcg agtaaagtag
      841 cactccagaa gacggttgtc atatacagct ttggccacaa tgcgtccgac aaacttgaag
      901 tagctgaggt ggttggggtt gcagtgggaa gatggattga tggtgtaggt gactcgatca
      961 ccaggtgagg tacggaacaa ggcatacata gggttaaaca tctctcgaga gatgatcata
     1021 taccactccc gcaggagccc gccagcatcc tgcc
//
