LOCUS       sequence-209033-        6484 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 400 / 490, Emission: 468 
            / 511.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6484)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 6484)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PS-CFP2-TBRII-N-18
FEATURES             Location/Qualifiers
     source          1..6484
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      537..546
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1873..1884
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             2373..3089
                     /codon_start=1
                     /product="photoswitchable cyan-to-green fluorescent 
                     protein"
                     /label=PS-CFP2
                     /note="mammalian codon-optimized"
                     /translation="MSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVATLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDDG
                     NYKTRAEVKFEGDTLVSRIELTGTDFKEDGNILGNKMEYNYNATNVYIVADKARNGIKV
                     NFKVRHNIKDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYLE
                     FVTAAAITHGMDELYK"
     polyA_signal    3212..3333
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3249..3268)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3303..3322
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3340..3795)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3477..3498)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3689..3708
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3822..3926
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3928..4285
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3928..4119
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3951..3971)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4136..4271
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4320..5114
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4320..5114
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4374..4393)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4984..5003
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5302..5321)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5346..5393
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5722..6310
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6211..6230
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcca
      541 ccatgggtcg ggggctgctc aggggcctgt ggccgctgca catcgtcctg tggacgcgta
      601 tcgccagcac gatcccaccg cacgttcaga agtcggatgt ggaaatggag gcccagaaag
      661 atgaaatcat ctgccccagc tgtaatagga ctgcccatcc actgagacat attaataacg
      721 acatgatagt cactgacaac aacggtgcag tcaagtttcc acaactgtgt aaattttgtg
      781 atgtgagatt ttccacctgt gacaaccaga aatcctgcat gagcaactgc agcatcacct
      841 ccatctgtga gaagccacag gaagtctgtg tggctgtatg gagaaagaat gacgagaaca
      901 taacactaga gacagtttgc catgacccca agctccccta ccatgacttt attctggaag
      961 atgctgcttc tccaaagtgc attatgaagg aaaaaaaaaa gcctggtgag actttcttca
     1021 tgtgttcctg tagctctgat gagtgcaatg acaacatcat cttctcagaa gaatataaca
     1081 ccagcaatcc tgacttgttg ctagtcatat ttcaagtgac aggcatcagc ctcctgccac
     1141 cactgggagt tgccatatct gtcatcatca tcttctactg ctaccgcgtt aaccggcagc
     1201 agaagctgag ttcaacctgg gaaaccggca agacgcggaa gctcatggag ttcagcgagc
     1261 actgtgccat catcctggaa gatgaccgct ctgacatcag ctccacgtgt gccaacaaca
     1321 tcaaccacaa cacagagctg ctgcccattg agctggacac cctggtgggg aaaggtcgct
     1381 ttgctgaggt ctataaggcc aagctgaagc agaacacttc agagcagttt gagacagtgg
     1441 cagtcaagat ctttccctat gaggagtatg cctcttggaa gacagagaag gacatcttct
     1501 cagacatcaa tctgaagcat gagaacatac tccagttcct gacggctgag gagcggaaga
     1561 cggagttggg gaaacaatac tggctgatca ccgccttcca cgccaagggc aacctacagg
     1621 agtacctgac gcggcatgtc atcagctggg aggacctgcg caagctgggc agctccctcg
     1681 cccgggggat tgctaacctc cacagtgatc acactccatg tgggaggccc aagatgccca
     1741 tcgtgcacag ggacctcaag agctccaata tcctcgtgaa gaacgaccta acctgctgcc
     1801 tgtgtgactt tgggctttcc ctgcgtctgg accctactct gtctgtggat gacctggcta
     1861 acagtgggca ggtgggaact gcaagataca tggctccaga agtcctagaa tccaggatga
     1921 atttggagaa tgttgagtcc ttcaagcaga ccgatgtcta ctccatggct ctggtgctct
     1981 gggaaatgac atctcgctgt aatgcagtgg gagaagtaaa agattatgag cctccatttg
     2041 gttccaaggt gcgggagcac ccctgtgtcg aaagcatgaa ggacaacgtg ttgagagatc
     2101 gagggcgacc agaaattccc agcttctggc tcaaccacca gggcatccag atggtgtgtg
     2161 agacgttgac tgagtgctgg gaccacgacc cagaggcccg tctcacagcc cagtgtgtgg
     2221 cagaacgctt cagtgagctg gagcatctgg acaggctctc ggggaggagc tgctcggagg
     2281 agaagattcc tgaagacggc tccctaaaca ctaccaaaag cagcggtgga gcaagcgcag
     2341 ccagtggtag cgcggatcca ccggtcgcca ccatgagcaa gggcgccgag ctgttcaccg
     2401 gcatcgtgcc catcctgatc gagctgaatg gcgatgtgaa tggccacaag ttcagcgtga
     2461 gcggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
     2521 ccggcaagct gcctgtgccc tggcccaccc tggtggccac cctgagctac ggcgtgcagt
     2581 gcttctcacg ctaccccgat cacatgaagc agcacgactt cttcaagagc gccatgcctg
     2641 agggctacat ccaggagcgc accatcttct tcgaggatga cggcaactac aagacgcgcg
     2701 ccgaggtgaa gttcgagggc gataccctgg tgagtcgcat cgagctgacc ggcactgatt
     2761 tcaaggagga tggcaacatc ctgggcaata agatggagta caactacaac gccaccaatg
     2821 tgtacatcgt ggccgacaag gccaggaatg gcatcaaggt gaacttcaag gtccgccaca
     2881 acatcaagga tggcagcgtg cagctggccg accactacca gcagaatacc cccatcggcg
     2941 atggccctgt gctgctgccc gataaccact acctgtccac ccagagcgcc ctgtccaagg
     3001 accccaacga gaagcgcgat cacatgatct acctcgagtt cgtgaccgcc gccgccatca
     3061 cccacggcat ggatgagctg tacaagtaaa gcggccgcga ctctagatca taatcagcca
     3121 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
     3181 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
     3241 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     3301 ttgtggtttg tccaaactca tcaatgtatc ttaaggcgta aattgtaagc gttaatattt
     3361 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa
     3421 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag
     3481 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
     3541 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga
     3601 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
     3661 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg
     3721 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc
     3781 cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     3841 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     3901 ttcaataata ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg
     3961 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     4021 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     4081 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     4141 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     4201 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     4261 ttttggaggc ctaggctttt gcaaagatcg atcaagagac aggatgagga tcgtttcgca
     4321 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     4381 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     4441 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     4501 aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     4561 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     4621 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     4681 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     4741 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     4801 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg
     4861 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     4921 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     4981 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     5041 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     5101 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct
     5161 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     5221 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     5281 ccaccctagg gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc
     5341 tatgacggca ataaaaagac agaataaaac gcacggtgtt gggtcgtttg ttcataaacg
     5401 cggggttcgg tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca
     5461 atacgcccgc gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg
     5521 gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc tcaggttact catatatact
     5581 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     5641 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     5701 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     5761 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     5821 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     5881 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     5941 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     6001 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     6061 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     6121 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     6181 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     6241 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     6301 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     6361 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgccat
     6421 gcattagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
     6481 tccg
//
