LOCUS       sequence-209069-        8968 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tet-regulated miR-E (miR-30 variant)-based RNAi.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8968)
  AUTHORS   Fellmann C, Hoffmann T, Sridhar V, Hopfgartner B, Muhar M, Roth M, 
            Lai DY, Barbosa IA, Kwon JS, Guan Y, Sinha N, Zuber J
  TITLE     An optimized microRNA backbone for effective single-copy RNAi.
  JOURNAL   Cell Rep. 2013 Dec 26;5(6):1704-13. doi: 
            10.1016/j.celrep.2013.11.020. Epub 2013 Dec 12.
  PUBMED    24332856
REFERENCE   2  (bases 1 to 8968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2013.11.020"; journalName: "Cell Rep"; date: 
            "2013-12-26- 26"; volume: "5"; issue: "6"; pages: "1704-13"
COMMENT     Sequence Label: LT3GEN
FEATURES             Location/Qualifiers
     source          1..8968
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             233..413
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    460..585
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1078..1311
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1496..1540
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1807..1924
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1940..2318
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    1948..1966
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1984..2002
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2020..2038
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2056..2074
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2092..2110
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2128..2146
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2164..2182
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2227..2251
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(2342..2344,2345..2347,2348..3061)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2387..2408)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2648..2667)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2995..3016
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        3335..3834
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3370..3393)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3767..3786
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3848..4651
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3911..3930)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4521..4540
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    4699..5287
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4752..4772)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5170..5181)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5494..5727
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5799..5920
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5836..5855)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5890..5909
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5939..6074
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6106..6125)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6107..6125)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6135..6152)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6135..6151)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6144..6166)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6296..6751
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6383..6402)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6593..6614
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6780..6884
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6885..6953,6954..7745)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7103..7122)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7916..8504
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8405..8424
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8658..8675
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8792..8813
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8828..8833,8834..8851,8852..8858)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8866..8882
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8871..8893
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8890..8906
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8890..8906
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8925..8945
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8927..8945
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacaataa acgggtctct
      241 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
      301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
      361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
      421 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
      481 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
      541 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
      601 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
      661 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
      721 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
      781 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
      841 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
      901 agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag
      961 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1021 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1081 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cctcaatgac
     1141 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1201 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1261 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1321 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1381 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     1441 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     1501 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     1561 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     1621 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     1681 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     1741 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     1801 gttaactttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     1861 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
     1921 ttttatcgat tctagattcg agtttactcc ctatcagtga tagagaacgt atgaagagtt
     1981 tactccctat cagtgataga gaacgtatgc agactttact ccctatcagt gatagagaac
     2041 gtataaggag tttactccct atcagtgata gagaacgtat gaccagttta ctccctatca
     2101 gtgatagaga acgtatctac agtttactcc ctatcagtga tagagaacgt atatccagtt
     2161 tactccctat cagtgataga gaacgtataa gctttaggcg tgtacggtgg gcgcctataa
     2221 aagcagagct cgtttagtga accgtcagat cgcctggagc aattccacaa cacttttgtc
     2281 ttataccaac tttccgtacc acttcctacc ctcgtaaagt cgagcttgcg ttggatccac
     2341 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     2401 cggcgacgta aacggccaca agttcagcgt gtctggcgag ggcgagggcg atgccaccta
     2461 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     2521 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
     2581 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     2641 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     2701 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     2761 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
     2821 cggcatcaag gcgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
     2881 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     2941 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
     3001 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta
     3061 atgacgtacg gcggccgcct cgactaggga taacagggta attgtttgaa tgaggcttca
     3121 gtactttaca gaatcgttgc ctgcacatct tggaaacact tgctgggatt acttcgactt
     3181 cttaacccaa cagaaggctc gagcaaccag aattcgagca attatcttgt ttactaaaac
     3241 tgaatacctt gctatctctt tgatacattt ttacaaagct gaattaaaat ggtataaatt
     3301 aaatcacttt tttcaattga cgcgtaattc taccgggtag gggaggcgct tttcccaagg
     3361 cagtctggag catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc
     3421 tggcctcgca cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc
     3481 cccttcgcgc caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc
     3541 gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac
     3601 agcaccgctg agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc
     3661 tccttcgctt tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc
     3721 gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt
     3781 caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc
     3841 agccaatatg ggatcggcca ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     3901 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     3961 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     4021 tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     4081 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     4141 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     4201 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     4261 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     4321 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     4381 ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     4441 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     4501 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     4561 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     4621 cttctatcgc cttcttgacg agttcttctg aggggatccg tcgacctgca gccaagctta
     4681 tcgaaacgcg tctggaacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     4741 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     4801 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     4861 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     4921 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     4981 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     5041 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg
     5101 tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     5161 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     5221 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     5281 tccccgcctg gaattaattc tgcagtcgag acctagaaaa acatggagca atcacaagta
     5341 gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg
     5401 tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag
     5461 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     5521 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
     5581 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     5641 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     5701 tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt
     5761 tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata
     5821 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     5881 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat
     5941 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     6001 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     6061 cttttttgga ggcctaggct tttgcgtcga gacgtaccca attcgcccta tagtgagtcg
     6121 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     6181 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     6241 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcgacgcg
     6301 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     6361 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     6421 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     6481 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     6541 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     6601 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     6661 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     6721 aattttaaca aaatattaac gtttacaatt tcccaggtgg cacttttcgg ggaaatgtgc
     6781 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     6841 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     6901 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     6961 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     7021 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     7081 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     7141 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     7201 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     7261 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     7321 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     7381 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     7441 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     7501 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     7561 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     7621 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     7681 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     7741 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     7801 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     7861 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     7921 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     7981 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     8041 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     8101 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     8161 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     8221 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     8281 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     8341 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     8401 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     8461 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     8521 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     8581 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     8641 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     8701 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     8761 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     8821 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     8881 aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact
     8941 aaagggaaca aaagctggag ctgcaagc
//
