LOCUS       sequence-209176-        9554 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Limb connective tissue specific inducible Cre.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9554)
  TITLE     Cre-inducible plasmids
REFERENCE   2  (bases 1 to 9554)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Prrx1:TFPnls-T2a-Cre-Ert
FEATURES             Location/Qualifiers
     source          1..9554
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     649..668
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        649..667
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(682..693,694..881)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     intron          3455..3587
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     3570..3589
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     3684..3703
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      3751..3760
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3757..4464
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
     CDS             4489..4509
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4513..4533
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4537..4557
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4579..4632
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4642..5664
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="NLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLLS
                     VCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLPR
                     PSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFLG
                     IAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVERW
                     ISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQRY
                     LAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLEDG
                     D"
     CDS             5677..6612
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             5725..6618
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATA"
     primer_bind     complement(6702..6721)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6767..6822
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6821..6840)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     misc_feature    join(7167..7304,7305..7316)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(7343..7363)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7343..7361)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7405..7421)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7405..7421)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7418..7440)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7429..7445
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7453..7459,7460..7477,7478..7483))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7498..7519
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7636..7653)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7807..8395)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7887..7906)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8566..9357,9358..9426))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9189..9208
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9427..9531)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgtagggat aacagggtaa tgcgcgcgta atacgactca
      661 ctatagggcg aattgggtac ccagaggtgt aaagtacttg agtaatttta cttgattact
      721 gtacttaagt attatttttg gggattttta ctttacttga gtacaattaa aaatcaatac
      781 ttttactttt acttaattac atttttttag aaaaaaaagt actttttact ccttacaatt
      841 ttatttacag tcaaaaagta cttatttttt ggagatcact tgggcccggc tcgagcatgc
      901 acgcgtgaat tcaataggag cctgtaatta cgttctagaa acctgtagca agaattcaat
      961 aggagcctgt aattacgttc tagaaacctg tagcaagaat tcttgctaca ggtttctaga
     1021 acaatggggg aggggaagtc agcaagtggc agtagttggt ttgcctctga cctttaggaa
     1081 aataccagac atttgcattt tctatatatg ctagatgcta tatttgttat tatctaaaca
     1141 aatttgctac acagatgtta gaaatgtatc taaatgttcc catagaaacc attaataccc
     1201 aggttgatac tgtagcaatt ggttgatagg aacatgggta accagctgcg gtttttcaaa
     1261 atgtgcctgc ttcctggaga aaaggcggaa tgcaaggaaa ggcacttaat gatatttctg
     1321 gggatggtct ctgatctctt ttccttgaca gtcttctgga gtatcaacca tactggctgc
     1381 aaacagaaac aaggatactt cttggaggca atatctaggg tgctagaagt cctgatcttt
     1441 tactcaggat ttggtcacaa atggcaatcc tcacgtctga aagaaaaagg aatccatctg
     1501 tgtaagtaaa gatggagcgc atgagcctcc agtctgagaa gatatgggag agactgatgc
     1561 tgtctggcct taggaagaga gaaagggtgt ctcctttccc ctaagggagc agagagttcc
     1621 agagacaccg gtggaaatat gccactttaa cctgaccatc gagtcgcttc cactccaggc
     1681 actttaggct ctaggagtct caccgttggc atctccgaaa gcacctcagg atactcaagt
     1741 ccccactctg tctggcccac ccacgggcaa gtccccagcg caagaaccag ctcttctggc
     1801 ttagatgccc tccggcagac aaaccttcgc ggctcagagc ctgagctgcc gagcaggggc
     1861 gaccagagca ctttggcggg gctgggggct gccggtcggg ggcacggcac gagtggctgg
     1921 agcgaggcga ggggtggaaa aaggaggggg cggcgggtgg actaatgatg aaaaagtctc
     1981 ctccatcctg gctccttaat taaatgcatg gaaagaaccg aggcgcgcac atctggtttc
     2041 aatctacagc cctttgatgg catcaaatgt tcttttccca gatcagggct ggaagttctg
     2101 ggctaactat ggccgtttgg agcacagaaa ccatttatac acactgggac ccttccttct
     2161 tttcaattca atctcatagc tacaggagaa ggggaaatct agcaatcagg ggagcgaggg
     2221 gaaacgtatg tatgtgtgtg tttaaagaac ataaaacgcc acaaataagc acatactatt
     2281 ttacttactg gccatatagt aactcattcc taatgagact attaaagctg ttaccagatt
     2341 ccaacaggtc atgaaaacac cgtccagcac cgctctgccc tctgtcaagc ctggtcatag
     2401 acttcgacct tcctcagtct ctgtgtttgc cagggcctca ccgaactgga tacagattag
     2461 cacagagccc accccacccc cagctcctcc tgctcccccc aacttcttca gccccagtgt
     2521 tcctgtctgc tctgatctgg ggcagcctcg gctacgagca agaaattaga acattggcaa
     2581 aggtagggga attatcagga cggactttct tctggtttaa tgaatatgta aattttctac
     2641 tgtctgaagc ctggggattt ctggtggcag cgaaagtctc gaatccattc gaagctgaag
     2701 acttctggat ctgcccaagc cgtctttaga tcgtagagag cctcccgtta ttatcgctct
     2761 tgttatttta ttacattatt taataggctg tctctacaga gccaaacgag ctttaatagg
     2821 cttgctcttt tttgccgttc agtctcagac tctccctgcg ctctccgcca aaacaaagct
     2881 gcaggaaaag ttactggggg gcggtgcagc cgcagatttg actgttatag atgtgcatct
     2941 tttccctgtt cctttttctt ttttcttttc ctaacaagtt tgccctcagc tgttatcaaa
     3001 acatgcaaat gagatattcc aagaggatct caaacaaatc cggaggagtt aatgctgaaa
     3061 caaaataatc agccccgttt taaagtgact gcgggtttcg ggcacttttc tctctgtgat
     3121 actactgcaa gggaatgata attagacctt tattttaaaa atccaaaggg gctctctcct
     3181 tagcttccct cccttttccc tccctccctc cttcctccct ccctcctcct ctcttgctcc
     3241 ctccctctcc ctttctctct ggctctgatg ttggcaaagg ggttttctta atcagactgt
     3301 tttttcggtc cgaggaaaag gaggtaggag attgtgatgg agaaaggggg tctgtaaaac
     3361 gtcaggcgcc gcacaaaggc tttgccacgt aattacaggc tcctattgaa ttcgaatttt
     3421 aagctgcaga agttggtcgt gaggcactgg gcaggtaagt atcaaggtta caagacaggt
     3481 ttaaggagac caatagaaac tgggcttgtc gagacagaga agactcttgc gtttctgata
     3541 ggcacctatt ggtcttactg acatccactt tgcctttctc tccacaggtg tccactccca
     3601 gttcaattac agctcttaaa attccgccac tagagcctct gctaaccatg ttcatgcctt
     3661 cttctctttc ctacagctcc tgggcaacgt gctggttatt gtgctgtctc atcattttgg
     3721 caaagaatta ttccgctagc gctaccggtc gccaccatgg tgagcaaggg cgaggagacc
     3781 acaatgggcg taatcaagcc cgacatgaag atcaagctga agatggaggg caacgtgaat
     3841 ggccacgcct tcgtgatcga gggcgagggc gagggcaagc cctacgacgg caccaacacc
     3901 atcaacctgg aggtgaagga gggagccccc ctgcccttct cctacgacat tctgaccacc
     3961 gcgttcgcct acggcaacag ggccttcacc aagtaccccg acgacatccc caactacttc
     4021 aagcagtcct tccccgaggg ctactcttgg gagcgcacca tgaccttcga ggacaagggc
     4081 atcgtgaagg tgaagtccga catctccatg gaggaggact ccttcatcta cgagatacac
     4141 ctcaagggcg agaacttccc ccccaacggc cccgtgatgc agaagaagac caccggctgg
     4201 gacgcctcca ccgagaggat gtacgtgcgc gacggcgtgc tgaagggcga cgtcaagcac
     4261 aagctgctgc tggagggcgg cggccaccac cgcgttgact tcaagaccat ctacagggcc
     4321 aagaaggcgg tgaagctgcc cgactatcac tttgtggacc accgcatcga gatcctgaac
     4381 cacgacaagg actacaacaa ggtgaccgtt tacgagagcg ccgtggcccg caattccacc
     4441 gacggcatgg acgagctgta caagtccgga ctcagatctc gagctgatcc aaaaaagaag
     4501 agaaaggtag atccaaaaaa gaagagaaag gtagatccaa aaaagaagag aaaggtagga
     4561 tccaccggat ctagacccga gggcagagga agtcttctaa catgcggtga cgtggaggag
     4621 aatcccggcc cactagtcga caatttactg accgtacacc aaaatttgcc tgcattaccg
     4681 gtcgatgcaa cgagtgatga ggttcgcaag aacctgatgg acatgttcag ggatcgccag
     4741 gcgttttctg agcatacctg gaaaatgctt ctgtccgttt gccggtcgtg ggcggcatgg
     4801 tgcaagttga ataaccggaa atggtttccc gcagaacctg aagatgttcg cgattatctt
     4861 ctatatcttc aggcgcgcgg tctggcagta aaaactatcc agcaacattt gggccagcta
     4921 aacatgcttc atcgtcggtc cgggctgcca cgaccaagtg acagcaatgc tgtttcactg
     4981 gttatgcggc ggatccgaaa agaaaacgtt gatgccggtg aacgtgcaaa acaggctcta
     5041 gcgttcgaac gcactgattt cgaccaggtt cgttcactca tggaaaatag cgatcgctgc
     5101 caggatatac gtaatctggc atttctgggg attgcttata acaccctgtt acgtatagcc
     5161 gaaattgcca ggatcagggt taaagatatc tcacgtactg acggtgggag aatgttaatc
     5221 catattggca gaacgaaaac gctggttagc accgcaggtg tagagaaggc acttagcctg
     5281 ggggtaacta aactggtcga gcgatggatt tccgtctctg gtgtagctga tgatccgaat
     5341 aactacctgt tttgccgggt cagaaaaaat ggtgttgccg cgccatctgc caccagccag
     5401 ctatcaactc gcgccctgga agggattttt gaagcaactc atcgattgat ttacggcgct
     5461 aaggatgact ccggtcagag atacctggcc tggtctggac acagtgcccg tgtcggagcc
     5521 gcgcgagata tggcccgcgc tggagtttca ataccggaga tcatgcaagc tggtggctgg
     5581 accaatgtaa atattgtcat gaactatatc cgtaacctgg atagtgaaac aggggcaatg
     5641 gtgcgcctgc tggaagatgg cgatctcgag ccatctgctg gagacatgag agctgccaac
     5701 ctttggccaa gcccgctcat gatcaaacgc tctaagaaga acagcctggc cttgtccctg
     5761 acggccgacc agatggtcag tgccttgttg gatgctgagc cccccatact ctattccgag
     5821 tatgatccta ccagaccctt cagtgaagct tcgatgatgg gcttactgac caacctggca
     5881 gacagggagc tggttcacat gatcaactgg gcgaagaggg tgccaggctt tgtggatttg
     5941 accctccatg atcaggtcca ccttctagaa tgtgcctggc tagagatcct gatgattggt
     6001 ctcgtctggc gctccatgga gcacccagtg aagctactgt ttgctcctaa cttgctcttg
     6061 gacaggaacc agggaaaatg tgtagagggc atggtggaga tcttcgacat gctgctggct
     6121 acatcatctc ggttccgcat gatgaatctg cagggagagg agtttgtgtg cctcaaatct
     6181 attattttgc ttaattctgg agtgtacaca tttctgtcca gcaccctgaa gtctctggaa
     6241 gagaaggacc atatccaccg agtcctggac aagatcacag acactttgat ccacctgatg
     6301 gccaaggcag gcctgaccct gcagcagcag caccagcggc tggcccagct cctcctcatc
     6361 ctctcccaca tcaggcacat gagtaacaaa ggcatggagc atctgtacag catgaagtgc
     6421 aagaacgtgg tgcccctcta tgacctgctg ctggaggcgg cggacgccca ccgcctacat
     6481 gcgcccacta gccgtggagg ggcatccgtg gaggagacgg accaaagcca cttggccact
     6541 gcgggctcta cttcatcgca ttccttgcaa aagtattaca tcacggggga ggcagagggt
     6601 ttccctgcca cagcttaaga tatcactcct caggtgcagg ctgcctatca gaaggtggtg
     6661 gctggtgtgg ccaatgccct ggctcacaaa taccactgag atctttttcc ctctgccaaa
     6721 aattatgggg acatcatgaa gccccttgag catctgactt ctggctaata aaggaaattt
     6781 attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag gacatatggg
     6841 agggcaaatc atttaaaaca tcagaatgag catttggttt agagtttggc aacatatgcc
     6901 catatgctgg ctgccatgaa caaaggttgg ctataaagag gtcatcagta tatgaaacag
     6961 ccccctgctg tccattcctt attccataga aaagccttga cttgaggtta gatttttttt
     7021 atattttgtt ttgtgttatt tttttcttta acatccctaa aattttcctt acatgtttta
     7081 ctagccagat ttttcctcct ctcctgacta ctcccagtca tagctgtccc tcttctctta
     7141 tgaagatccc tcgaccagcg gccgctaata ctcaagtaca attttaatgg agtacttttt
     7201 tacttttact caagtaagat tctagccaga tacttttact tttaattgag taaaattttc
     7261 cctaagtact tgtactttca cttgagtaaa atttttgagt actttttaca cctctgccgc
     7321 ggtggagctc cagcttttgt tccctttagt gagggttaat tgcgcgcatt accctgttat
     7381 ccctacgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     7441 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     7501 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     7561 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     7621 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     7681 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     7741 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     7801 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     7861 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     7921 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     7981 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     8041 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     8101 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     8161 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     8221 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     8281 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     8341 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     8401 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     8461 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     8521 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     8581 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     8641 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     8701 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     8761 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     8821 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     8881 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     8941 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     9001 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     9061 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     9121 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     9181 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     9241 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     9301 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     9361 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     9421 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     9481 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     9541 ccgaaaagtg ccac
//
