LOCUS       sequence-209215-       20795 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 20795)
  AUTHORS   Potter CJ, Tasic B, Russler EV, Liang L, Luo L
  TITLE     The Q system: a repressible binary system for transgene expression, 
            lineage tracing, and mosaic analysis.
  JOURNAL   Cell. 2010 Apr 30. 141(3):536-48.
  PUBMED    20434990
REFERENCE   2  (bases 1 to 20795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2010 
            Apr 30. 141(3):536-48."
COMMENT     Sequence Label: pBac-GH146-QF
FEATURES             Location/Qualifiers
     source          1..20795
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   746..808
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     regulatory      1287..1296
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1293..1970
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(1473..1493)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     1885..1908
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    2091..2172
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2128..2147)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2182..2201
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2241..2257
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(4373..4393)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     repeat_region   18121..18155
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(18646..18662)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(18646..18662)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(18659..18681)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    18670..18686
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(18694..18700,18701..18718,18719..18724))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    18739..18760
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(18877..18894)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(19048..19636)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(19128..19147)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(19807..20598,20599..20667))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     20430..20449
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(20668..20772)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcctcgtt cattcacgtt tttgaacccg tggaggacgg
      661 gcagactcgc ggtgcaaatg tgttttacag cgtgatggag cagatgaaga tgctcgacac
      721 gctgcagaac acgcagctag attaacccta gaaagataat catattgtga cgtacgttaa
      781 agataatcat gtgtaaaatt gacgcatgtg ttttatcggt ctgtatatcg aggtttattt
      841 attaatttga atagatatta agttttatta tatttacact tacatactaa taataaattc
      901 aacaaacaat ttatttatgt ttatttattt attaaaaaaa acaaaaactc aaaatttctt
      961 ctataaagta acaaaacttt tatcgaattc ctgcagcccg ggggatccac tagttctagt
     1021 gttcccacaa tggttaattc gagctcgccc ggggatctaa ttcaattaga gactaattca
     1081 attagagcta attcaattag gatccaagct tatcgatttc gaaccctcga ccgccggagt
     1141 ataaatagag gcgcttcgtc tacggagcga caattcaatt caaacaagca aagtgaacac
     1201 gtcgctaagc gaaagctaag caaataaaca agcgcagctg aacaagctaa acaatcgggg
     1261 taccgcgggc ccgggatcca ccggtcgcca ccatggcctc ctccgagaac gtcatcaccg
     1321 agttcatgcg cttcaaggtg cgcatggagg gcaccgtgaa cggccacgag ttcgagatcg
     1381 agggcgaggg cgagggccgc ccctacgagg gccacaacac cgtgaagctg aaggtgacca
     1441 agggcggccc cctgcccttc gcctgggaca tcctgtcccc ccagttccag tacggctcca
     1501 aggtgtacgt gaagcacccc gccgacatcc ccgactacaa gaagctgtcc ttccccgagg
     1561 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggcgacc gtgacccagg
     1621 actcctccct gcaggacggc tgcttcatct acaaggtgaa gttcatcggc gtgaacttcc
     1681 cctccgacgg ccccgtgatg cagaagaaga ccatgggctg ggaggcctcc accgagcgcc
     1741 tgtacccccg cgacggcgtg ctgaagggcg agacccacaa ggccctgaag ctgaaggacg
     1801 gcggccacta cctggtggag ttcaagtcca tctacatggc caagaagccc gtgcagctgc
     1861 ccggctacta ctacgtggac gccaagctgg acatcacctc ccacaacgag gactacacca
     1921 tcgtggagca gtacgagcgc accgagggcc gccaccacct gttcctgtag cggccgcgac
     1981 tctagatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
     2041 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
     2101 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     2161 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct cgaggggggg
     2221 cccggtaccg ggccccccct cgaggtcgac ggtatcgata agcttgatgg actatagttg
     2281 agggcattcg gattgccacc ggctgccagg atggctgcat tcttcttgtg cttttgcatg
     2341 tgcgaggtta gagcagcagc tgtattatag cccctgttgc acatgctgca ctggaagggc
     2401 ttctgtatgt catgggtttt catgtggatc ttcaagtggt cactagagaa agatgaaata
     2461 gaggaaattt attgacttag ttaataggtc acatattaaa ccagactccc tcacttacct
     2521 cctggaaaaa gctgcctcgc aatgcggaca cttgtagttc cgctcgttgg tatgcagttt
     2581 cttatgacga tctcgggatc gcttgtgctt gaagagcttg gagcagtatt cacatttgaa
     2641 aggcaggtgt tcagcgtggc tctgtaatga atatacacat attcggaaaa tcccattagc
     2701 atgatgataa tctgagcaat tagccgtagg cccactgggc tatttgaaat tcttagaaga
     2761 gcaccagaca acaaggcagc gtcaactgtc cgtctacccc agttaaaagt tgcgatgaca
     2821 acgatgacga ctacgacgac gacttggacg actcctggct tgaatttata gatgcgtcaa
     2881 tctgaatctg aatctgcata ccgactgact cgactcggac tcaggcccag acgagtaggt
     2941 gtttcacttg cgactcgtgg cttgtggaca aaattcatct aaatgcgcga cgccgcttac
     3001 tgtttttttt gttttttttt tctttcgcag gttgggctgg aaaaggggca ggtgctgggg
     3061 ctggggttgg gtcttaaact acacccgcaa ccggtttgct ttttgggttc ttttagcggc
     3121 agccggcttt gaactttgag agcggcgaaa aaaaaaatta gcccatttgt atgcagcccc
     3181 ccagtttgga aagccttaaa attgcacaat atatgatgag ttagccgggt tagtcgcatc
     3241 cagatccaca gagagcgtac aacttctcgt gttggttatg gttttttaaa ctgtcgctcc
     3301 aatcaccatc gccatcaaca tcccataagc tgaaagaccg aattccgaac cgtaccagcc
     3361 catccaggcg ctaggatcaa agccggatcg caacatgtta gaccccggtg gacgataaag
     3421 atcgctgctc ggtagtcctc aacagcagtt gcaattgggt taacaagttg ctgatgtcat
     3481 gggcggcggt gttagcagtt caaaaagcat ttaagccgaa ttagaatttt cgctcgcgga
     3541 gatgaggggg catgtgggtg aagtgcggtg gccacaaagg ctcaaagctt cgggttgtct
     3601 tagatgttgt tttagagatt gccgaaggag tgttagataa atgatatagg tctgcaattt
     3661 ttaaataatt cacttgatcc taatttgttt catttggaag atttcagaga acgtaccttt
     3721 attttacata tattgttcaa aggagaaacg agccttgcgt acatgctaga tttgacattt
     3781 ccacttaaac aaccctttac atctaagagc tatgccactt tcgctttcaa ttaacaaatt
     3841 gaaatcgcag tttggttcct attcactcga tggaaatgaa aatcaatgga accaccgcag
     3901 attgtcaatc aaagtcctga caccctgagt gcctaaaggt gtcctaagta tttagaagac
     3961 tgtcaccaaa ggggcaaatg tcaatcaaga gaaaacacct tgctcgaaaa tagaataatc
     4021 gccttaaaaa gatgtcagga tggtaattac gaactttcaa agccacccac ttgtggtgga
     4081 gaccacccac ccactcaccc tttctagact ggagttcctt cgaactttga acccactcaa
     4141 tttggtaact cacctgtacg tgatgcttca agtagccgag acgtggaaat gtcttttcgc
     4201 aaaactggca ctggtaggat ccgtcgccgc gacttttaat cccctctcct gtcaacttat
     4261 caaacttgct tgctgctcca gtggctcccc cacctgcggt ggctcccatt ttggcttcct
     4321 tattgatttt gcgcagcttg ttggctattt tcagcgcctg ttgatgatgg taatgatgat
     4381 gatggtgatg atggaagtgt ggcaaacgtt ttggcttggg caaatcctcg ccatcagcac
     4441 caccattgat cttgccctca gtcggatgat cctcctgacc ggcggcggtt ggagctccga
     4501 tcaagtggtg tgaattgggc gtcatggtgc cgtcagaaat gctaggcgaa taactggacg
     4561 atgtgtgggt gctgtacata tctggaagga gaaaatgaag acacggatta gtaccaagaa
     4621 aataggatca gtgtcactta ggggtaaaac gtcttacatc ggtcttacat ataaaaatat
     4681 tatgataaga gtagaacaaa ttaggaaatt atgtttcaat ttattataga gttgcttatt
     4741 taacgaagaa aggcaattgg gaacattgcg ttcccaataa tggtctttct atcatgggaa
     4801 gcggtttttt tatgagacta tattccatct tatcgagtgg gcggctgtgg ctgcatcctg
     4861 tttagcggcg ctttggggac cgggacagga aatcaacgtc gactatcaaa tgctggacgc
     4921 ggaattaatt caattaaccg ggcaacattt ggtgatgacc agacgacacc ccattcccca
     4981 tacatgggta tgagctgtaa gcgagtctca tgcaaactaa ttgcgctttg ttcttgctgc
     5041 tgttttaatt acaggcccca gcatccatga gggtcacttg attccccccg gattcgggct
     5101 tctattaaca cgcgccaagt gccgatgttc actttccgtt ttcgacaact tttaattaag
     5161 cccaattgcg catgatcttc gcgtctgtat ctgtttggat aatgctctgg gtcgagggat
     5221 cgtggttgtt ttaccccccc gatcggttga actgtgatct atctggtcta agttccactc
     5281 taattgggaa acatcaagct gatgcatcag ccacttgatt ccttcattcg tcttgatctt
     5341 tggggttaaa gaaagttgta agagatttaa agccattcgc atttgattga aatgttggct
     5401 atggttaagt taagggggta agaaagtcaa gtgtcaaagc ggttaaacgg attggagaag
     5461 attgatgtag atgttctagg atttatggca aaagttgaaa attatacaat tcgtttcgat
     5521 tttcgtggaa attttgaaat atatatttta gaatgaataa gttttatgat ccagatgtaa
     5581 gcaaaatgaa agctgtttat ttttactgac atcatataaa ttgattgatt ttccgtcggc
     5641 acattgcaca ttggatacaa gctttttata agctttgcta tttacactgt ttggttttaa
     5701 aggaccccaa tggtcggacg cctaattaat ccatcaagaa ctataacttt ttttggaatt
     5761 ttcccaaatt ctttttggat atccttgcta tttaccgtta ttggtgatct ccttggtggc
     5821 ctggatcttt tcaattaaat tctccaaacg cgaactgggt accagtattg tcttatttaa
     5881 cttctagtta atttatttaa ttggatttga actaaattcg attgaggagg cactttagct
     5941 gcactttctc gataaacacc aagcactttc agtttaggcc cttgctgcac caaaggatca
     6001 ttatatctct attctgatgt atgggttgcc agcgtttttt ttttctttca aagcgcgagg
     6061 cgcgagacga aggacgtcca attgccgcga gcaccaaaat gtggctaaaa tcagagccgc
     6121 agagccaaag gccgagtgcc aaagagccca acggagaaag acagagagag ggagagggag
     6181 agagagagtg tgaggaagca gacggaagga gagggcgaca actaggcgaa ctagtatgat
     6241 gaaataacat aaggtggtcc cgtcgatagc cgaagcttac cgaagtatac acttaaattc
     6301 agtgcacgtt tgcttgttga gaggaaaggt tgtgtgcgga cgaatttttt tttgaaaaca
     6361 ttaaccctta cgtgcggccg ctaagcaaat aaacaagcgc agctgaacaa gctaaacaat
     6421 ctgcagccca agctggccgg ccaaaatgcc gcctaaacgc aagacactca atgccgctgc
     6481 cgaagccaat gcccacgctg atggccatgc tgatggcaat gctgatggtc atgtcgctaa
     6541 cactgcagca agcagcaaca acgcccgttt tgcggacttg accaacattg acacacccgg
     6601 cctcggccct accacgacga cgttacttgt cgagcccgct cgttcgaaac gccagagagt
     6661 ctcgagggcc tgtgatcagt gtcgagctgc acgtgaaaag tgtgatggaa tccagccggc
     6721 ttgcttcccc tgtgtgtcgc agggccggtc gtgtacctac caggccagtc ccaagaagcg
     6781 aggagtccag acgggctaca tccgcactct cgaactggct ctggcttgga tgttcgagaa
     6841 cgttgcccgc agcgaggacg ccctccacaa tcttttggtc cgtgatgctg gccagggcag
     6901 cgctctcctg gtcggcaaag actcgcctgc tgcagaacgc ctgcatgcaa gatgggcgac
     6961 gagtcgagtc aacaaaagca tcacccgtct tctctcaggt caggccgcac aagatccatc
     7021 tgaagacggc caatccccgt ccgaagacat aaatgtccaa gatgccgggg caaagacatc
     7081 cgacttccct catgcgcctc acttgacttt ctcggcgcct aagtctagta cagctgagac
     7141 acgcactcta ccaggcccgg tccgaccacc tatttcggca aacaccctgg aaaacaacct
     7201 tcagccagat ggtaccggga tagggaagct accacccaat cattggcgcc tgctggatat
     7261 ctacttttcc tacacgcatt cttggctccc tatcctcgag aagaaagaca tgtaccaagc
     7321 attgtaccag tactctgaac aaggctcgtt gcttccctct gcgaatgtcg agtctggcgt
     7381 tcatgccgag ctctggagcg cgctcgccct ggcgtccttc caggctgctg ctactgctgc
     7441 atcgagtgct acgggtccag cttcagctgc tcatggccat gacaatgcca tcaatccttc
     7501 acctgcagac atatctgaca cagcccgaaa gctcatacct ttggaaagcg ggccgttcca
     7561 ggttcagcac tgcagagcgt tgctgcttct ttgtctcgta agccttgggc gggatgattg
     7621 ggagtctgct tggttgctgg ttggctttgc ggtccgcgtc ctacttgttg ttcgcaccca
     7681 gttgcctcct gatgatgacc ggccacgacc aagaatgcgt gcgctgctcg tcgcgtgctt
     7741 catcgtggat accattgtgt ctatgagaca caacgtgccg gcccatctca agccagacga
     7801 cattgcggat ctgccgttac ctgaagacgg tcaagatcaa tgggagccgt ggacaccatg
     7861 tgagggctta ggcggtgaac acaccatgct gcaaatgttg aggaacccgg catacccttt
     7921 aagcacattc aaccacctat atggcgtgac caagctggtt gctttggagc ttctgccaag
     7981 aatacgaaca tcttcacaga acgctccctt ggagttcagg tcgcggttgc agcaggtaat
     8041 cggccacaat tctcccttca gcgtctttgt cctttcccag gatacagcat cggcttttgt
     8101 gcctactgca taccttaccc gtaccgttta tttatgggca gctgcctttt ctgagcctct
     8161 caacgaacac tactcgcatc ttctgatcga aactcttgat cagtatcaga agcggtttgg
     8221 tacatatgca atcccacctc tgatcccttc tcttctagac tcccttcttg ctttaaagaa
     8281 acaatcacat tcttcagagc ggcatcgaag gcacttggaa gagcttttcc ccgcctactc
     8341 ctccatttgg cctcggggag gccgacacag caatactggc ctccaaccca tacgacaact
     8401 tgagcttccg ccgactgcga ctgccactgc aagtatcatg ccccatgtca tggaacagcc
     8461 cctgtcaacg tcaataaatc cggtcaatga tcggtttaat ggaataccga atcctacccc
     8521 ctacaatagc gatgcggccc tagacgcaat tactcaaacc aatgactacg gatcagtcaa
     8581 tacccatggc attctcagta catatccacc acctgccacc cacttgaacg aggcctcggt
     8641 ggcccttgcg cctggaggtg ctcctcccag accacccccg ccatacgttg atagtacaac
     8701 gaaccatcct ccttaccata gtaacctcgt ccccatggcc aatttcggat attcgactgt
     8761 agactatgat gcgatggtgg atgatctggc atcgatcgag tacacggatg cggttgacgt
     8821 tgatccgcag ttcatgacga acctcgggtt tgtcccaggg tgtaatttta gcgatatcaa
     8881 cacgtatgag caataggcgg ccgcgttaac cgatccgtcg accatgaagc ctagggctta
     8941 tcgataccgt cgactaaagc caaatagaaa attattcagt tcctggctta agtttttaaa
     9001 agtgatatta tttatttggt tgtaaccaac caaaagaatg taaataacta atacataatt
     9061 atgttagttt taagttagca acaaattgat tttagctata ttagctactt ggttaataaa
     9121 tagaatatat ttatttaaag ataattgcgt ttttattgtc agggagtgag tttgcttaaa
     9181 aactcgttta gatccactag ttttttcgcc tctgtgtgcg tgccttttgg gattctcggc
     9241 caacaaatga gaagcaacac acagcgaacg taatttaaat ggcgccccca ccccaccccc
     9301 actaatacac acacgaacgc gcacgcacac gggcgagagt gagaaagctt cgtgtgtata
     9361 taagcaccac acactgatat cccgaagtaa aagccgaaga tgttgcgttc gtgggcgaga
     9421 gcgagatggc gccacataga gtgcgcaaaa gagagagggc gaatttgtcg tatctgtgtt
     9481 ggagttggtg cccccaccga acacatagac gctatatagc cacatataca ccactacact
     9541 ggtgctggcg aagggcggag catgggatcc aatgggtcga cgcggccaga gggttccctg
     9601 atgggtcaaa ggaggggggt tgcggatgga agcgggatgg caaatgcgtt ttatacttaa
     9661 tttatgtgca cataaaaatt taatttaccc aaaacgtcag gtgatgatgt gatatttcac
     9721 tacttttggc tcgttttccc cttttttgtg gtttttgttg ttcatttttt ttttggcgag
     9781 atttgacttt gatgaaggaa ttagcttttt gggttaatta cttgctatgg gtatagaaag
     9841 tttttatatt caatacataa agtataactt ttcctaaagt aaataaagtt ggtttttgga
     9901 agtttaaata ataaatacta ataataaata atagtgcggg gcagaccctc agccgtggaa
     9961 gctaccgatc aattttgttt gtgggcgtgg caatgttctt accagcaaag gcgacatttg
    10021 aatctgcata cttgatcttg aactttctat aaattttagt caacgagatc acgacgttcg
    10081 agttccaact tcaagttgtg atcaagttgt gattcttatg aagaatacac acttataggg
    10141 gaggaaatgt ttccttctac ctgttaatac ctttaagcgt attcagtttt actctacgag
    10201 tttaatttta aactttaatt caaccaacct ttgccaaact ttttgactgg cacaaatgtg
    10261 gtaatttgta tacatttcca taaaaatcca ggcaaaccga agtgtgtaaa tgtcaattgg
    10321 cactttttgc tcatatcaac atatgaacat gaccgcaaat aaacactaca caggcaatag
    10381 aaaaagtaca aagttaaatt gacgtcagaa acgagatgat aaaaacgaaa gccatgcggg
    10441 aaaaacacgt taaagccatt agcgcgtacc cgtcaatgtg gcaagcgaat tgttgcgaaa
    10501 agcaattgtg atgccggaga atatagcagg acaccgcatc cggtggccag gacgaggaca
    10561 acacacaatg cactgaaagc caaattaatt atgcatacgg cggcggcagt gacagccagc
    10621 tggaaaaacg tcagcaaagt gcagcaaata aaagctgaat tcgtgcgaac tgtcaacagc
    10681 agaagttcac ttagagaaaa atatgcatgt cttgcgacca cttacattcc tatctatgtt
    10741 tcataagtaa tttgaaagta tttaagaagt ataactattt cccgcaaaat agaaattcaa
    10801 taaaatggtt tcagtatttt taaataggat aactaataga tttgattaaa tagcataaac
    10861 tgaaatataa acctcttcaa tttatttaac atttcaatga aaacgggatt tgggccttca
    10921 ttaatatttt atcctaacaa taagtgtaca tattttaatg ccttttttaa tgtgtatcta
    10981 ttcttaagct ccgcactgtg cacattgcac ctgtcctctc ctttctttta gcccaaatcc
    11041 tttgtcagtt gccgtaaaac tattaaagca attaagaaat cacttttcac tattttagcc
    11101 ccactctaac ttttgttttt tcctttttta ctaagcttat ttttacaatt ttctttatac
    11161 cttaactcct attggtggct gactgagttg gccaatcgca gcgctctctg ccggcgcatg
    11221 cgtcgctgtt accgcttttg cacaacgaaa caaaaaaaca aaaacaaaaa caaccagagc
    11281 cagtacatga gcggcacata tagaaagaga tggctatcga tggagctgtc gattcgggtc
    11341 tctcggctgt taacagttaa ctctcgattt gctaattgac gtcgccaggt ttgtcgtttg
    11401 agtctttggt ttgaggttgt gcaatggttc gtggctacat tcaggccgtt aacgattgca
    11461 tttctacggg acttttgaaa ataaattttg tattctttta agtttgtttg gttgtaaaat
    11521 atttatgtat tgaagataaa ttttcgttgt aaggattaat attaaataag ttctatttta
    11581 ataaatcaat aagtcgaatg agttcttaaa attaatctta aattcacttc ggtatattgt
    11641 tatattttcg tgtaacctca acactttcat aattaatact ttaattaaag taatttattt
    11701 tgtattattt atcgtacgcc atttgcttaa tagagcttaa ctgtcaattc ctcaaagcta
    11761 accacttcgt tgtcccaaaa acccttttcg aacatcattg tcccattata gcggttttca
    11821 attacaaata cttccaatta acctaataac gggcaccaga aagctttaac ctttgacctc
    11881 cctcaaacaa aactttgtcg caagtcaaaa gtcggaaaaa acccacaaaa cgaatttatc
    11941 aacggcaatc ctaagatcaa attgcaatta gaattaaatt ttcgctttct acatatttaa
    12001 tgacagacga acacaagtag aagttttccg tgcgacaaca attttcgtaa ttgtaagagg
    12061 aaatccggat tttccatttt aaattctaat tgcccttaga tgttatttat gtccattcac
    12121 attttcccca atttcccatt tggccgttga aaaatcaact ttcggctgaa aaatgttaaa
    12181 agttacccat acccgctcgc cactcgacat tgagccactt tagtcctcca tctccggtgt
    12241 taattacgtt tgagtgacag ttttgtgaca ggccacgata agtgggtagg atggttggga
    12301 tttttgggtg gtggtgcggt agttgggcgg tggtgggtga aagggtgtcg gcacaggtaa
    12361 attgtgtctc ccagcctggg ctgcccgatc cttttcctgt gcattttaaa tgcgcggcta
    12421 atgcaaagtc ggcccgaact tttcaccgag atttccagat ttgcagttgg tcagcagcag
    12481 gacctcatgt gtcactcttg ccgtccttga tatgtgtatg tgtgtgtgta agcgtgtgtg
    12541 tttgctgaca gccaaagggt ttcgccagcg ccctcatgca taatagataa attataaaaa
    12601 tatatgccct gcttgggagt tgggtaggtg gaaaaggaga ggcctgtcag catgtcatcc
    12661 attgtcctgc cgccagccaa agtgtccttt tgtttcagcg cattttaaat tagccattct
    12721 cggaaaaagt cactcaaatt gtcactttga tgtcctgaca gctgcccaga ccccagttcc
    12781 aagccaaaca aaaaaaaaac ccaaaaacca tcatcggtcc taagtccgag ttctatgtgt
    12841 ctccacagat ttccatcttt ccgtgatctc attccggcgc taacggcgca attaatcagt
    12901 cgcttgagga cacttttaat tagtgccgca atcatttggt ggtcaagatt gggaccaaaa
    12961 aaaggtttat tcatgtcaaa ggatcatttt taatgaaagc tgacattatt tgtggactta
    13021 tgcgctctgt gtggctgatg attgaaattt taaatgaaag cccaacaaaa tgaatagaaa
    13081 ttatgcaagc atatggctct tcacctactt ctgtaaaaag tcgcatcctt cctggtggcc
    13141 actctactcc actcagctct tctttcacct ggaacccaac tgcgttgccc tccccgcatt
    13201 agagacaact cttagacata tttaccaaac aaacaattat cctcgtaatc tgatttcgat
    13261 gatgacccct ctttggcata ttgtcaagtc atgcccgctc aatggtccat aatgatgatc
    13321 ccttcgaatt gcatcacaca cgtatctcga tctccaagtt actcaatgaa aaatgaagtt
    13381 cctcaatggt ggacaaggag agccgaggat ttgtctcaat tgggatcgag gcgctgacac
    13441 aaatcaatca gtcggtgcag tcagcaggta gaagtgatcg gggatcagtc tgggccagcg
    13501 atcttgatcg actgtcaagg ggtaatgatg ggtctggcga cgtgtcgagg gcgtcctgcg
    13561 gtggctaaat ccgctttctt gaaaagggtt tctcaaatcg atttcgatga cgctctgccc
    13621 aaccgaccat aattgacttt cgcacctgtt ttgtggacgc actagctctt actctctctc
    13681 ttgccatgga atgaaaaccc attgaaccgc agaaatgtgg ggatagtggg gaaatgtgcg
    13741 gtgcggtggc acgtgctacc cagttttgtg gctttaactg ggagccagaa caatgcccca
    13801 cagctgccac tcctaaacgg tctttgacca acaagccatc aaaatcgcac gcgtgccagc
    13861 cgccataaac ttggagggtc tgagacggcg tccagaaaat tgaaatgcat tgcattgccg
    13921 tcggatggag ggaggtggaa gtggaagtaa gtgaagtgaa gtccagaaac cagattaaca
    13981 tgcccgattg ccacatccga ggtcttttgt acttgctccg attccgattc cggtcgctga
    14041 tatctactac gctggagttc ttcaactcca ctcggaagac ggaaactaga acttcacggc
    14101 gattcaagtg gcatgataac ggggttcact cggaattggg tttacgacac ttttttcctg
    14161 ctactggttt gtgtttggca ccgctgatgg agataataat cataaaataa ttttattgca
    14221 aaggctgttg ctacctcact tttggcattt aactaataag ttggagggga attgtggcgg
    14281 gtcaatgatg atgaaggatg ctgagggtga ggacagatga tggggtccag ctggaggtca
    14341 ttgtcaatga attattaacg acatttctct aagggcaagg aggcagccag gattaaatat
    14401 ttgggtacat cccacattaa atatagtttt tcaaaccaac tctaataaaa gcctttggta
    14461 tctaataagt taaactgtta aaagctaatg ggagttatca atagtgctgc aaataactcc
    14521 ttcactcaac tactaattgg atacttaact cgtaacgtta ttatccaaat tttcaaccca
    14581 attctccgga caccaaaatc cccagacaaa cagatggggt gactctgaac accacaaaga
    14641 tttataagca tgttacctgc tgagtctgct tgcccataac aatatgtcta tattaaatcc
    14701 tttaagatca aataaccaaa gcagcagcca tatcgcatgt gaaacatttc aacgcgtatt
    14761 tcatttaaat taccccaaat ttatccccga aaaacgagac gagccacaga gtatttcact
    14821 gtgtgtgtgt gtgtgtgtgt gtgtgatgga gaaaagggcg gaaagccgga gctgaaaggg
    14881 ttcccctggg attttcgtac gcggaattgc gccttggagt cgcataagga cgaggagcaa
    14941 cccagaacta tgcacatggg agccaccaaa aaaaaaaaac caaggagcaa tgtgggtggt
    15001 aaaaatgcga tacacgaacg cttggctgct tttatttttg catcgtaaaa ggaaatgaaa
    15061 acgtctttcg cggaaagttt gcttttccag tgcggccgac accgcgcgcg ggaatgggtc
    15121 caaaaaactt ggcgaaaggc ggacgaacca ccaaaggcgc ataaatcatc caaggtggca
    15181 atagcagcag caacttgggc gtgtcataac tttggagcca gaaacccagt tggccatagc
    15241 ccgtgggatc gaaaacaatg aggatggagc cggcgaaagt tggtacaaaa aaaattgcca
    15301 agagtcctgg ccaatgcgcc tatggtgggt tacaggtaaa aaaaaaaaac gtagaatgcg
    15361 gcaacgtcct gcaactgaca ctgcagtccg agctacgccg agtgccagga tgcaggacca
    15421 acttttttcg ccttgtttcc gcttgtttag ctgcggtgtc agggcggttt tacacctgag
    15481 attgcgatta ctttgtccgg acaggcgggc aagttgggtt aaagttttcg agatccctat
    15541 accctaaggt aagtgggtgc ctgcatttcg tggtatggct ttaaaaacta tgtgcattaa
    15601 aggacatgag cttagttagg aatgtactca taaaatgcac tcaaatagta ttgctaaatt
    15661 gaatcattgt ggatcattat ctattatatt ttcaagatat gctaaccatt ttttaatgag
    15721 catgccaaca aacttagcta atcaactgaa caacgaacac ttctaagtaa tttaatacct
    15781 aaataaaaga tatatgataa attcaatgaa gccaaaaatg gtttgaagag tgtgtgaatg
    15841 tcaccgcccc ctttttttgg gtggctcctc cccctgagag ccgcgtaaag cagcgaaaac
    15901 tttctaatga cttgtcggcg gtgtaaggaa ctttagttgt ggacattttg caggtccgga
    15961 gctgtaatta aaacgcatta gatttatact taggatagcc cggtgaccca gttacgcgac
    16021 ggtggctgga tcttcgtgag ggctgtgtgc cctaaagagc cggagagttc tctctctacg
    16081 tgatcatcgc attgatcccg tagtagtctt ttggcccttt tgagtgccac acactaagct
    16141 gaagcgaagc ggttcattaa ccgtggcgat gtcatcttgc cgggttaacc agttttcccc
    16201 aaacacagat ttctatctcc gtgtgcggtg cgtaaagcaa tttgcgctga tgatgagacg
    16261 cgacttcttg ggaaagcaac ggttcaagtg ctgtgagaat ttgccacttt aagcaatccc
    16321 caagactcaa gccgtctcga ctccattcga cccacgaaag aaactgacat cccaagctca
    16381 aaaaaccttt gaccaaagca attatttatg gcctcgagac acgaggaagg ggataactct
    16441 ctgtttgtct tgtaagacaa atgtgtaatt aattgcgtcg catcaaaagg aagctctaaa
    16501 actatttgca tacgagcatg gcaaattgtc ttcgctgtct catcttgaac tacacgtgca
    16561 attaataaat caaaagggac agcctaagcc cagcgtgtcc tttcgtcctt ccgagcggaa
    16621 gtctcgggcc aggccaagac aaacaaatga agtgactgga accgaaaaac tttgccaact
    16681 taaggtattc ttttctgcaa gtgcccggat tcgaagttcg gcgggccaag agacgggcgt
    16741 tccactgcaa ctaattgcat ttcattgcat acatttaggt tgccccaccg aatcctttgg
    16801 ctccttgctt tcgcttgcag ccgcaatcac ttcgcacaca caacgtaaaa cgtttcaaaa
    16861 ttgtgctcaa atagccagtg agatttcctt ggagctgaca aatgggttga ggtggaaagc
    16921 caaccacaag tgcctcgagt ccttaaaggg ggagggcaag aggagtcaag gatgccagga
    16981 aggatgcgct ggccaagtgt tgagtgctag ctatgcttgc actgggagaa cttaaaattg
    17041 ctttgttaac gttttagttg ttcaaaaact attcaagttt tatggtaaag aaacttagaa
    17101 gactaatact tataaccttc gatcagcctt aattagtctt agttttattt acctttacct
    17161 atttctattt tatgttattt tcgtaaagtg tagaggaagg ataagacaga caagttagca
    17221 gccgggcaaa tttagctccg gagcaggagg caagtagtga agtcaagtga agactgagtt
    17281 gtgtgcatga atgaatgaat gaaagggggc ggcaatgggc agcccgttca gaatgctgcc
    17341 gccggcaagg acgttgttcg tggcaggaag agcaaaaagc acaagaagga agttgtgaaa
    17401 aaatagcagt cgccacaaaa cgaaacaaat aacacagaaa aagcattcgg aatggcacta
    17461 gagagaaatg tggccaagtg tgagggaacg gtagatggtt cacaggatgg ttggatggct
    17521 ggatggctgg atggttggat ggtcggatgg ttgaatggtt gcggggggta tggcggtgag
    17581 gtcatgtcgt ggccgtagct catgatgctg ccgggtctcc atttctggca tctctgtctg
    17641 tctctcagtc tcttcgacag ctcgtaaatg gcagccagga aagtgatggc acaaggggga
    17701 actgggaggg agtaggagga tactctggtc ccaacttgac tccgaatgct ggcccgagtt
    17761 cagcttggtg ttgattgctg tttggggttt ttaaaacgta gttaggctgc ccggcagcca
    17821 caggaccgca gcatatggaa acgacaccca tctataacaa gaaaatatat atataataag
    17881 ttatcacgta agtagaacat gaaataacaa tataattatc gtatgagtta aatcttaaaa
    17941 gtcacgtaaa agataatcat gcgtcatttt gactcacgcg gtcgttatag ttcaaaatca
    18001 gtgacactta ccgcattgac aagcacgcct cacgggagct ccaagcggcg actgagatgt
    18061 cctaaatgca cagcgacgga ttcgcgctat ttagaaagag agagcaatat ttcaagaatg
    18121 catgcgtcaa ttttacgcag actatctttc tagggttaat ctagctgcat caggatcata
    18181 tcgtcgggtc ttttttccgg ctcagtcatc gcccaagctg gcgctatctg ggcatcgggg
    18241 aggaagaagc ccgtgccttt tcccgcgagg ttgaagcggc atggaaagag tttgccgagg
    18301 atgactgctg ctgcattgac gttgagcgaa aacgcacgtt taccatgatg attcgggaag
    18361 gtgtggccat gcacgccttt aacggtgaac tgttcgttca ggccacctgg gataccagtt
    18421 cgtcgcggct tttccggaca cagttccgga tggtcagccc gaagcgcatc agcaacccga
    18481 acaataccgg cgacagccgg aactgccgtg ccggtgtgca gattaatgac agcggtgcgg
    18541 cgctgggata ttacgtcagc gaggacgggt atcctggctg gatgccgcag aaatggacat
    18601 ggataccccg tgagttaccc ggcgggcgcg cttggcgtaa tcatggtcat agctgtttcc
    18661 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
    18721 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
    18781 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
    18841 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
    18901 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
    18961 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
    19021 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
    19081 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
    19141 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
    19201 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
    19261 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
    19321 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
    19381 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
    19441 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
    19501 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
    19561 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
    19621 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
    19681 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
    19741 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
    19801 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
    19861 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
    19921 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
    19981 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
    20041 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
    20101 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
    20161 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
    20221 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
    20281 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
    20341 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
    20401 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
    20461 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
    20521 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
    20581 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
    20641 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
    20701 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
    20761 aggggttccg cgcacatttc cccgaaaagt gccac
//
