LOCUS       sequence-209241-        6445 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of mRIN2 dRA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6445)
  AUTHORS   Sandri C, Caccavari F, Valdembri D, Camillo C, Veltel S, 
            Santambrogio M, Lanzetti L, Bussolino F, Ivaska J, Serini G
  TITLE     The R-Ras/RIN2/Rab5 complex controls endothelial cell adhesion and 
            morphogenesis via active integrin endocytosis and Rac signaling.
  JOURNAL   Cell Res. 2012 Oct;22(10):1479-501. doi: 10.1038/cr.2012.110. Epub 
            2012 Jul 24.
  PUBMED    22825554
REFERENCE   2  (bases 1 to 6445)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/cr.2012.110"; journalName: "Cell Res"; date: "2012-10"; 
            volume: "22"; issue: "10"; pages: "1479-501"
COMMENT     Sequence Label: HA-mRIN2 dRA
FEATURES             Location/Qualifiers
     source          1..6445
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        97..400
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        401..604
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     555..575
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     601..625
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          740..835
                     /label=CMV intron
                     /note="modified intron A from human cytomegalovirus (CMV)"
     CDS             856..882
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             862..888
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     polyA_signal    3269..3390
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3306..3325)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3360..3379
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3397..3852)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3534..3555)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3746..3765
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3879..3983
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3985..4342
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3985..4176
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4008..4028)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4193..4328
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4377..5171
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4377..5171
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4431..4450)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5041..5060
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5359..5378)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5403..5450
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5779..6367
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6268..6287
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgattctg tggataaccg tattaccgcc atgcattagt tattaatagt aatcaattac
       61 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
      121 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
      181 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
      241 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
      301 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
      361 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
      421 catcaatggg cgtggatagc agtttgactc acggggattt ccaagtctcc accccattga
      481 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa
      541 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
      601 agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca
      661 tagaagacac cgggaccgat ccagcctccg cggccgggaa cggtgcattg gaacgcggat
      721 tccccgtgcc aagagtgacg taagtaccgc ctatagactc tataggcaca cccctttggc
      781 tcttatgcat gctgacagac taacagactg ttcctttcct gggtcttttc tgcaggcacc
      841 gtcgacttaa caatgtaccc atacgatgtt ccggattacg ctagcctccc agatctcgac
      901 ccattttgca ttctcagcat ggtgagacac aaggatggtg gctactctga agacaaggat
      961 gggaagacat gtccccgaga ctctggctat gacagcctct ccaacaggct cagcattctg
     1021 gaccggctcc tgcataccca tcccatatgg ctgcagctga gtctgagtga ggaggaggca
     1081 gcagaggtcc tgcaggccca gcctccaggg atctttttgg ttcggaaatc ctccaaaatg
     1141 cagaagaaag tcctctccct tcgcctgccc tgtgaatttg gagcacctct caaagagttt
     1201 actataaagg aaagcaccta caccttttcc ctggaaggct caggaatcag ctttgcagat
     1261 ttattccggc ttattgcctt ctactgcatc agcagggacg ttttgccatt caccttgaag
     1321 ctgccttatg ccatttcaac agccaagacg gagtctcagc ttgaagaact agcccagctg
     1381 ggactaaatt tttggagctc ctcagcagac aacaaacccc cgaactcccc acctccccat
     1441 aggcctcttc cctcagccgg catctgccct gcctccttgc gtcagctctg ccttataaat
     1501 ggagtgcatt ctataaaaac caggacgcct tcggagctgg agtgcagcca gaccaacgga
     1561 gccttgtgtt ttattaatcc tcttttcttg aaagtgcaca gccaggacct cagcacaggc
     1621 ccaaagcggc ccagcacaag gactcccaac gcgaatggca ccgagagacc tcggtccccc
     1681 ccacccaggc ccccgccacc cgctattaat agtctgcaca caagccctgg gctatccagg
     1741 actgaacccc agacgagcat gccagaaaca gtcaaccata gcaaacatgg gaacgtagct
     1801 ctgcttggaa caaaaccaac tcccatccct ccaccccggc tgaagaagca ggcttctttt
     1861 ttggaagcag aaagcagcgc caagaccttg actgcccgcc ggcccagccg gagctcggag
     1921 ccggagcccg agctagagct ggagttagag atgggcacag ctgggcacgc aggtggagcc
     1981 ccgcctaggg acgcccccgg ggattgcaca agggcgccgc cgcccggctc tgaatcacaa
     2041 ccaccaccgt gccatggagc caggcagagg ctgagcgaca tgagcctttc cacttcctcc
     2101 tccgactcgc tggagttcga ccggagcatg cctctgtatg ggtacgaggc cgacaccacc
     2161 agcagcctgg aggactacga aggggagagt gaccaagaga ccatggcacc tcccatcaag
     2221 tccaaaaaga agaggaacag ctcttttgtg ctgcccaagc ttgtcaagtc ccagttgcga
     2281 aagatgagcg gggtgttcag ctccttcatg acccctgaga agcggatggt ccgcaggatc
     2341 gccgagctgt cccgggacaa atgcacctat tttgggtgct tggtgcagga ctacgtgagc
     2401 ttcctgaagg agaacaagga gtgccatgtg tccagcaccg atatgctgca gaccatccgg
     2461 cagtttatga cccaggtcaa gaactatttg tctcagagct ctgaactgga cccccctatt
     2521 gagtcactga tcccagaaga ccaaattgat gtggtgttgg agaaggccat gcacaaatgt
     2581 atcttgaagc ccctcaaggg ccatgtggag gccatgctga aggacttcca cacggctgac
     2641 ggctcgtgga aacaactcaa ggaaaaccta caactcgtgc ggcagaggaa cccacaggag
     2701 ctgggggtct ttgccccaac ccctgacttg atggagctgg agaaaatcaa gcttaaattc
     2761 atgaccatgc agaagatgta ttcgccagaa aagaaagtca tgcttttgct acgggtctgt
     2821 aagctcatct acactgtcat ggagaacaac tcagggagga tgtacggtgc agacgacttc
     2881 ttgccagtcc tgacctatgt catagctcag tgtgacatgc ttgaactgga cacggaaatc
     2941 gagtacatga tggaactcct ggacccatca ctgctgcacg gagaaggagg ctattacttg
     3001 accagtgcct atggggcgct atctctgata aagaatttcc aagaagaaca ggctgcgaga
     3061 ctgctcagct ccgaagccag ggacacgctg aggcagtggc acaagcggag aaccaccaac
     3121 cgaactatcc cctccgttga cgactaggcg gccgcgactc tagatcataa tcagccatac
     3181 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
     3241 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
     3301 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     3361 tggtttgtcc aaactcatca atgtatctta aggcgtaaat tgtaagcgtt aatattttgt
     3421 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg
     3481 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt
     3541 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
     3601 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt
     3661 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
     3721 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc
     3781 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
     3841 tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     3901 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     3961 aataatattg aaaaaggaag agtcctgagg cggaaagaac cagctgtgga atgtgtgtca
     4021 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     4081 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     4141 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     4201 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     4261 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     4321 tggaggccta ggcttttgca aagatcgatc aagagacagg atgaggatcg tttcgcatga
     4381 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
     4441 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
     4501 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag
     4561 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
     4621 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
     4681 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
     4741 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
     4801 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
     4861 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg
     4921 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
     4981 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
     5041 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
     5101 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
     5161 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc
     5221 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     5281 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     5341 ccctaggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat
     5401 gacggcaata aaaagacaga ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg
     5461 ggttcggtcc cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata
     5521 cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct
     5581 cgcagccaac gtcggggcgg caggccctgc catagcctca ggttactcat atatacttta
     5641 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     5701 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     5761 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     5821 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     5881 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     5941 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     6001 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     6061 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     6121 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     6181 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     6241 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     6301 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     6361 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     6421 tcacatgttc tttcctgcgt tatcc
//
