LOCUS       sequence-209283-        1520 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Shen H, Campanello GC, Flicker D, Grabarek Z, Hu J, Luo C, Banerjee 
            R, Mootha VK
  TITLE     The Human Knockout Gene CLYBL Connects Itaconate to Vitamin B12.
  JOURNAL   Cell. 2017 Nov 2;171(4):771-782.e11. doi: 
            10.1016/j.cell.2017.09.051. Epub 2017 Oct 19.
  PUBMED    29056341
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.09.051"; journalName: "Cell"; date: "2017-11-2-
            2"; volume: "171"; issue: "4"; pages: "771-782.e11"
COMMENT     Sequence Label: His-PaIct
FEATURES             Location/Qualifiers
     source          1..1520
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             31..48
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             55..99
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             1364..1381
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(1434..1452)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1448..1495
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt ctggtgccac gcggttctgg tatgaaagaa
       61 accgctgctg ctaaattcga acgccagcac atggacagcc cagatctggg taccatgacc
      121 cagagtcatc gtccgcgccc gctggatggc attaccgtgg ttagcctgga gcatgccatt
      181 gccgccccgt tttgcacacg tcagctggcc gatctgggtg cccgtgtgat caaaattgaa
      241 cgtccgggtg tgggcgattt tgcccgcggc tatgatgaac gcgttaacgg tctgagcagc
      301 cattttgtgt ggacaaaccg cagcaagcag agcctgagcc tggatttaaa gcaggccgaa
      361 gcactgccgc tgctggaaaa actgctggcc ggcgccgatg ttctggttca gaatctggca
      421 ccgggtgccg cagcccgtct gggtctgagc tatgaagcct tacgtgaacg ctttccgcgt
      481 ctgatcgtgt gcgatattag cggctacggc gagggtggtc cgtacgagaa gaagaaagcc
      541 tacgacctgc tgattcagag cgaaggcggt tttctgagcg tgaccggtgg cgcaggcccg
      601 aatgaaatgg ccaaagccgg ctgcagcatc gcagacattg ccgccggcat gtacgcctac
      661 agtagtattc tgagcgccct gctgttacgc gaacgtaccg gtgaaggtag ccgtatcgat
      721 gtgagcatgc tggaaagtct ggtggaatgg atgggctacc cgatgtatta tgcctaccag
      781 gatgccccgc cgccgccgcg cgccggtgcc agccatagta ccatctaccc ttatggcccg
      841 ttcccggcag gtgatggtgg tacagttatg ctgggtctgc agaacgagcg tgagtggcgt
      901 gcattttgcg acaaagtgct gcgccgtccg gaactggccg atgacgaacg cttcagcgcc
      961 aatttcaaac gcagtgccaa ccgtgaggaa ctgcgcgcac tgatcgttga ggcctttgca
     1021 gcactgccgg ccgcagaagt tgttgcccgc ctggaacagg cacaaatcgc cagcgcccat
     1081 gtgaatgata tggccggtgt ttgggcccat ccgcaactgc aggcccgtca gcgctggaca
     1141 agcattgata gcccggccgg tcgtctgcct gccttattac cgccggcaag cagcagtgcc
     1201 tttgttccgc gcatggatgc agtgccggca ctgggtggcg atagtgatgc cctgctggcc
     1261 gaactgggtt gcacagccgc cgatattcag cgtctgcgcg caacaggcac tgtttgatga
     1321 tgagaattcg agctccgtcg acaagcttgc ggccgcactc gagcaccacc accaccacca
     1381 ctgagatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga
     1441 gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa
     1501 aggaggaact atatccggat
//
