LOCUS       sequence-20937-e         614 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 614)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG304GAL-ccdB-DsRed
FEATURES             Location/Qualifiers
     source          1..614
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(45..169)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(177..193)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(177..193)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(214..237)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
ORIGIN
        1 ggaagcataa agtggtaaag cctgggggtg cctaatgcgg ccgccatagt gactggatat
       61 gttgtgtttt acagtattat gtagtctgtt ttttatgcaa aatctaattt aatatattga
      121 tatttatatc attttacgtt tctcgttcag cttttttgta caaacttgtg atgggggatc
      181 cactagttct agaatccggg gttttttctc cttgacgtta aagtatagag gtatattaac
      241 aattttttgt tgatactttt attacatttg aataagaagt aatacaaacc gaaaatgttg
      301 aaagtattag ttaaagtggt tatgcagttt ttgcatttat atatctgtta atagatcaaa
      361 aatcatcgct tcgctgatta attaccccag aaataaggct aaaaaactaa tcgcattatc
      421 atcctatggt tgttaatttg attcgttcat ttgaaggttt gtggggccag gttactgcca
      481 atttttcctc ttcataacca taaaagctag tattgtagaa tctttattgt tcggagcagt
      541 gcggcgcgag gcacatctgc gtttcaggaa cgcgaccggt gaagacgagg acgcccggag
      601 gagagtcttc cttc
//
