LOCUS       sequence-209403-        9085 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expressing Ca sensor yellow cameloen 3.6 under guard cell specific 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9085)
  AUTHORS   Yang Y, Costa A, Leonhardt N, Siegel RS, Schroeder JI
  TITLE     Isolation of a strong Arabidopsis guard cell promoter and its 
            potential as a research tool.
  JOURNAL   Plant Methods. 2008 Feb 19;4:6. doi: 10.1186/1746-4811-4-6.
  PUBMED    18284694
REFERENCE   2  (bases 1 to 9085)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods."; date: "2008-02-19"; volume: "4:6. doi"; pages: " 
            10.1186/1746-4811-4-6"
COMMENT     Sequence Label: pGC1::YC3.60
FEATURES             Location/Qualifiers
     source          1..9085
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(1719..1721,1722..1724,1725..2237)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIE"
     CDS             2859..2936
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     CDS             2943..3677
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="MDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRD
                     HMVLLEFVTAAGITLGMDELYKGGSGGMVSKGEELFTGVVPILVELDGDVNGHKFSVSG
                     EGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPE
                     GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
                     VYITADKQKNGIKANFKIRHNIE"
     terminator      3701..3953
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3962..3978)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4026..4046)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4026..4044)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4065..4081)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4065..4081)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4078..4100)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4089..4105
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4113..4119,4120..4137,4138..4143))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4158..4179
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4296..4313)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4418..4442
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(4533..5121)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4613..4632)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5292..6107)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(5292..6107)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     6015..6034
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     6099..6118
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      6398..6833
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    6960..6982
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     polyA_signal    6990..7166
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(7398..8423)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(8443..8788)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(8480..8500)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     primer_bind     9006..9022
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9032..9051
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9032..9050
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gagtaaagat tcagtaaccc gatgctcctg ctcttcctca agaccttcct tgattcgccg
       61 ccggtatgtt ctccgtctgt ggtagcgcct ttggaacact ctaccaacgc cgccatgaaa
      121 ggatctctca tggccgcagg ggacgtgttc ttcttacatc tggtgttagg gctatggtta
      181 ctccagtgag gagggagagg caagaggttg cttaatgatt cgtttttccg gtgatacgag
      241 aactctttag gtttaccggg aagcttttcc catgaaaatg ggatgccaag tggatggaga
      301 ggagttgccg gagagttgcc ggagaatagg agggaattgg aggaggagga agagagtgat
      361 cgccgggttg aaatgttaac cgtcgaggag aatttgaccg agttggatcg tctagtaggt
      421 acaattcggg tccttggcga agtatccatt caaaatagtg tttagttttg gacttgagaa
      481 cttgttgtct ctttgatctc ttttatataa aactttggac gtgtaggaca aacttgtcaa
      541 cataagaaac aaaatggttg caacagagag gatgaattta taagttttca acaccgcttt
      601 tcttattaga cggacaacaa tctatagtgg agtaaatttt tatttttggt aaaatggtta
      661 gtgaattcaa atatctaaat tttgtgactc actaacatta acaaatatgc ataagacata
      721 aaaaaaagaa agaataattc ttatgaaaca agaaaaaaaa cctatacaat caatctttag
      781 gaattgacga tgtagaattg tagatgataa attttctcaa atatagatgg gcctaatgaa
      841 gggtgccgct tattggatct gacccatttt gaggacatta atattttcat tggttataag
      901 ccttttaatc aaaattgtca ttaaattgat gtctccctct cgggtcattt tcctttctcc
      961 ctcacaatta atgtagactt tagcaatttg cacgctgtgc tttgtcttta tatttagtaa
     1021 cacaaacatt ttgacttgtc ttgtagagtt tttctctttt atttttctat ccaatatgaa
     1081 aactaaaagt gttctcgtat acatatatta aaattaaaga aacctatgaa aacaccaata
     1141 caaatgcgat attgttttca gttcgacgtt tcatgtttgt tagaaaattt ctaatgacgt
     1201 ttgtataaaa tagacaatta aacgccaaac actacatctg tgttttcgaa caatattgcg
     1261 tctgcgtttc cttcatctat ctctctcagt gtcacaatgt ctgaactaag agacagctgt
     1321 aaactatcat taagacataa actaccaaag tatcaagcta atgtaaaaat tactctcatt
     1381 tccacgtaac aaattgagtt agcttaagat attagtgaaa ctaggtttga attttcttct
     1441 tcttcttcca tgcatcctcc gaaaaaaggg aaccaatcaa aactgtttgc atatcaaact
     1501 ccaacacttt acagcaaatg caatctataa tctgtgattt atccaataaa aacctgtgat
     1561 ttatgtttgg ctccagcgat gaaagtctat gcatgtgatc tctatccaac atgagtaatt
     1621 gttcagaaaa taaaaagtag ctgaaatgta tctatataaa gaatcatcca caagtactat
     1681 tttcacacac tacttcaaaa tcactactca agaaatccat ggtgagcaag ggcgaggagc
     1741 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaggt
     1801 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
     1861 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctggg
     1921 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     1981 ccatgcccga aggctacgtc caggagcgta ccatcttctt caaggacgac ggcaactaca
     2041 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
     2101 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacatca
     2161 gccacaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc cacttcaaga
     2221 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
     2281 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc
     2341 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
     2401 cccgcatgca tgaccaactg acagaagagc agattgcaga gttcaaagaa gccttctcat
     2461 tattcgacaa ggatggggac ggcaccatca ccacaaagga acttggcacc gttatgaggt
     2521 cgcttggaca aaacccaacg gaagcagaat tgcaggatat gatcaatgaa gtcgatgctg
     2581 atggcaatgg aacgatttac tttcctgaat ttcttactat gatggctaga aaaatgaagg
     2641 acacagacag cgaagaggaa atccgagaag cattccgtgt ttttgacaag gatgggaacg
     2701 gctacatcag cgctgctcag ttacgtcacg tcatgacaaa cctcggggag aagttaacag
     2761 atgaagaagt tgatgaaatg ataagggaag cagatatcga tggtgatggc caagtaaact
     2821 atgaagagtt tgtacaaatg atgacagcaa agggggggaa gaggcgctgg aagaaaaact
     2881 tcattgccgt cagcgctgcc aaccggttca agaagatctc cagctccggg gcactggagc
     2941 tcatggacgg cggcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     3001 gccccgtgct gctgcccgac aaccactacc tgagctacca gtccgccctg agcaaagacc
     3061 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     3121 tcggcatgga cgagctgtac aagggtggca gcggtggcat ggtgagcaag ggcgaggagc
     3181 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
     3241 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagctga
     3301 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgggctacg
     3361 gcctgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     3421 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
     3481 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
     3541 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
     3601 gccacaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc aacttcaaga
     3661 tccgccacaa catcgagtaa gaattcgatc gaatttcccc gatcgttcaa acatttggca
     3721 ataaagtttc ttaagattga atcctgttgc cggtcttgcg atgattatca tataatttct
     3781 gttgaattac gttaagcatg taataattaa catgtaatgc atgacgttat ttatgagatg
     3841 ggtttttatg attagagtcc cgcaattata catttaatac gcgatagaaa acaaaatata
     3901 gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct atgttactag atcgggaatt
     3961 cactggccgt cgttttacaa cgtcgtgacg cggccgccac cgcggtggag ctccagcttt
     4021 tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat catggtcata gctgtttcct
     4081 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     4141 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     4201 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     4261 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     4321 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     4381 gaatcagggg ataacgcagg aaagaacatg aaggccttga caggatatat tggcgggtaa
     4441 actaagtcgc tgtatgtgtt tgtttgagat ctcatgtgag caaaaggcca gcaaaaggcc
     4501 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     4561 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     4621 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     4681 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     4741 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     4801 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     4861 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     4921 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     4981 tttggtatct gcgctctgct gaagccagtt accttcggaa gaagagttgg tagctcttga
     5041 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     5101 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     5161 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     5221 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgtgtaacat
     5281 tggtctagtg attagaaaaa ctcatcgagc atcaaatgaa actgcaattt attcatatca
     5341 ggattatcaa taccatattt ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg
     5401 aggcagttcc ataggatggc aagatcctgg tatcggtctg cgattccgac tcgtccaaca
     5461 tcaatacaac ctattaattt cccctcgtca aaaataaggt tatcaagtga gaaatcacca
     5521 tgagtgacga ctgaatccgg tgagaatggc aaaagtttat gcatttcttt ccagacttgt
     5581 tcaacaggcc agccattacg ctcgtcatca aaatcactcg catcaaccaa accgttattc
     5641 attcgtgatt gcgcctgagc gagacgaaat acgcgatcgc tgttaaaagg acaattacaa
     5701 acaggaatcg aatgcaaccg gcgcaggaac actgccagcg catcaacaat attttcacct
     5761 gaatcaggat attcttctaa tacctggaat gctgttttcc ctgggatcgc agtggtgagt
     5821 aaccatgcat catcaggagt acggataaaa tgcttgatgg tcggaagagg cataaattcc
     5881 gtcagccagt ttagtctgac catctcatct gtaacatcat tggcaacgct acctttgcca
     5941 tgtttcagaa acaactctgg cgcatcgggc ttcccataca atcggtagat tgtcgcacct
     6001 gattgcccga cattatcgcg agcccattta tacccatata aatcagcatc catgttggaa
     6061 tttaatcgcg gccttgagca agacgtttcc cgttgaatat ggctcataac accccttgta
     6121 ttactgttta tgtaagcaga cagttttatt gttcatgatg atatattttt atcttgtgca
     6181 atgtaacatc agagattttg agacacaacg tggctttgtt gaataaatcg aacttttgct
     6241 gagttgaagg atcagatcac gcatcttccc gacaacgcag accgttccgt ggcaaagcaa
     6301 aagttcaaaa tcaccaactg gtccacctac aacaaagctc tcatcaaccg tggctccctc
     6361 actttctggc tggatgatgg ggcgattcag gcgatcccca tccaacagcc cgccgtcgag
     6421 cgggcttttt tatccccgga agcctgtgga tagagggtag ttatccacgt gaaaccgcta
     6481 atgccccgca aagccttgat tcacggggct ttccggcccg ctccaaaaac tatccacgtg
     6541 aaatcgctaa tcagggtacg tgaaatcgct aatcggagta cgtgaaatcg ctaataaggt
     6601 cacgtgaaat cgctaatcaa aaaggcacgt gagaacgcta atagcccttt cagatcaaca
     6661 gcttgcaaac acccctcgct ccggcaagta gttacagcaa gtagtatgtt caattagctt
     6721 ttcaattatg aatatatata tcaattattg gtcgcccttg gcttgtggac aatgcgctac
     6781 gcgcaccggc tccgcccgtg gacaaccgca agcggttgcc caccgtcgag cgcctttgcc
     6841 cacaacccgg cggccggccg caacagatcg ttttataaat tttttttttt gaaaaagaaa
     6901 aagcccgaaa ggcggcaacc tctcgggctt ctggatttcc gatccccgga attagatctt
     6961 ggcaggatat attgtggtgt aacgttatcg atctggattt tagtactgga ttttggtttt
     7021 aggaattaga aattttattg atagaagtat tttacaaata caaatacata ctaagggttt
     7081 cttatatgct caacacatga gcgaaaccct ataagaaccc taatttccct tatcgggaaa
     7141 ctactcacac attatttatg gagaaaatag agagagatag atttgtagag agagactggt
     7201 gatttcagcg taccgaatta attcctgaat cactgcgacc ggccctcccg cgacccagcc
     7261 gagcgagctt agcgaactgt ggacgagaac tgtgccacca agcgtaaggc cgttctctcg
     7321 catttgcctt gctaggctcg cgcgagttgc tggctgaggc gttctcgaaa tcagctcttg
     7381 ttcggtcggc atctactcta ttcctttgcc ctcggacgag tgctggggcg tcggtttcca
     7441 ctatcggcga gtacttctac acagccatcg gtccagacgg ccgcgcttct gcgggcgatt
     7501 tgtgtacgcc cgacagtccc ggctccggat cggacgattg cgtcgcatcg accctgcgcc
     7561 caagctgcat catcgaaatt gccgtcaacc aagctctgat agagttggtc aagaccaatg
     7621 cggagcatat acgcccggag ccttggcgat cctgcaagct ccggatgcct ccgctcgaag
     7681 tagcgcgtct gctgctccat acaagccaac cacggcctcc agaagaagat gttggcgacc
     7741 tcgtattggg aatccccgaa catcgcctcg ctccagtcaa tgaccgctgt tatgcggcca
     7801 ttgtccgtca ggacattgtt ggagccgaaa tccgcgtgca cgaggtgccg gacttcgggg
     7861 cagtcctcgg cccaaagcat cagctcatcg agagcctgcg cgacggacgc actgacggtg
     7921 tcgtccatca cagtttgcca gtgatacaca tggggatcag caatcgcgca tatgaaatca
     7981 cgccatgtag tgtattgacc gattccttgc ggtccgaatg ggccgaaccc gctcgtctgg
     8041 ctaagatcgg ccgcagcgat agcatccata gcctccgcga ccggctgaag aacagcgggc
     8101 agttcggttt caggcaggtc ttgcaacgtg acaccctgtg cacggcggga gatgcaatag
     8161 gtcaggctct cgctgaactc cccaatgtca agcacttccg gaatcgggag cgcggccgat
     8221 gcaaagtgcc gataaacata acgatctttg tagaaaccat cggcgcagct atttacccgc
     8281 aggacatatc cacgccctcc tacatcgaag ctgaaagcac gagattcttc gccctccgag
     8341 agctgcatca ggtcggagac gctgtcgaac ttttcgatca gaaacttctc gacagacgtc
     8401 gcggtgagtt caggcttttt catagggggg atcagcttgg gctgtcctct ccaaatgaaa
     8461 tgaacttcct tatatagagg aagggtcttg cgaaggatag tgggattgtg cgtcatccct
     8521 tacgtcagtg gagatgtcac atcaatccac ttgctttgaa gacgtggttg gaacgtcttc
     8581 tttttccacg atgctcctcg tgggtggggg tccatctttg ggaccactgt cggcagaggc
     8641 atcttgaatg atagcctttc ctttatcgca atgatggcat ttgtaggagc caccttcctt
     8701 ttctactgtc ctttcgatga agtgacagat agctgggcaa tggaatccga ggaggtttcc
     8761 cgaaattacc ctttgttgaa aagtctcaat agccctttgg tcttctgaga ctgtatcttt
     8821 gacatttttg gagtaggggt acgataacat taacgcttac aatttccatt cgccattcag
     8881 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
     8941 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
     9001 acgttgtaaa acgacggcca gtgagcgcgc gtaatacgac tcactatagg gcgaattggg
     9061 taccgggccc cccctcgagg tcgac
//
