LOCUS       sequence-209519-        6758 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  HLA-A0201 his tagged.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6758)
  AUTHORS   Lampen MH, Uchtenhagen H, Blom K, Varnaite R, Pakalniene J, 
            Dailidyte L, Walchli S, Lindquist L, Mickiene A, Michaelsson J, 
            Schumacher TN, Ljunggren HG, Sandberg JK, Achour A, Gredmark-Russ S
  TITLE     Breadth and Dynamics of HLA-A2- and HLA-B7-Restricted CD8(+) T Cell 
            Responses against Nonstructural Viral Proteins in Acute Human 
            Tick-Borne Encephalitis Virus Infection.
  JOURNAL   Immunohorizons. 2018 Jul 2;2(6):172-184. doi: 
            10.4049/immunohorizons.1800029.
  PUBMED    31022685
REFERENCE   2  (bases 1 to 6758)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.4049/immunohorizons"; journalName: "Immunohorizons"; date: 
            "2018-07-2- 2"; volume: "2"; issue: "6"; pages: "172-184"
COMMENT     Sequence Label: pMP71-HLA-A0201-His
FEATURES             Location/Qualifiers
     source          1..6758
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..25
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6..24
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    56..80
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1196..1213
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    complement(1239..1263)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    1508..1911
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     LTR             2417..3007
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(3032..3048)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3032..3048)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3045..3067)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3056..3072
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3080..3086,3087..3104,3105..3110))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3125..3146
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3263..3280)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3434..4022)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(4193..4984,4985..5053))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4816..4835
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5054..5158)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5226..5244
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5282..5304)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5404..5423
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5617..5639
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5631..5648
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5632..5648
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tcgagtaata cgactcacta tagggagacc caagctggct aggtaagctt gatcaacaag
       61 tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc atggccgtca tggcgccccg
      121 aaccctcgtc ctgctactct cgggggctct ggccctgacc cagacctggg cgggctctca
      181 ctccatgagg tatttcttca catccgtgtc ccggcccggc cgcggggagc cccgcttcat
      241 cgcagtgggc tacgtggacg acacgcagtt cgtgcggttc gacagcgacg ccgcgagcca
      301 gaggatggag ccgcgggcgc cgtggataga gcaggagggt ccggagtatt gggacgggga
      361 gacacggaaa gtgaaggccc actcacagac tcaccgagtg gacctgggga ccctgcgcgg
      421 ctactacaac cagagcgagg ccggttctca caccgtccag aggatgtatg gctgcgacgt
      481 ggggtcggac tggcgcttcc tccgcgggta ccaccagtac gcctacgacg gcaaggatta
      541 catcgccctg aaagaggacc tgcgctcttg gaccgcggcg gacatggcag ctcagaccac
      601 caagcacaag tgggaggcgg cccatgtggc ggagcagttg agagcctacc tggagggcac
      661 gtgcgtggag tggctccgca gatacctgga gaacgggaag gagacgctgc agcgcacgga
      721 cgcccccaaa acgcatatga ctcaccacgc tgtctctgac catgaagcca ccctgaggtg
      781 ctgggccctg agcttctacc ctgcggagat cacactgacc tggcagcggg atggggagga
      841 ccagacccag gacacggagc tcgtggagac caggcctgca ggggatggaa ccttccagaa
      901 gtgggcggct gtggtggtgc cttctggaca ggagcagaga tacacctgcc atgtgcagca
      961 tgagggtttg cccaagcccc tcaccctgag atgggagccg tcttcccagc ccaccatccc
     1021 catcgtgggc atcattgctg gcctggttct ctttggagct gtgatcactg gagctgtggt
     1081 cgctgctgtg atgtggagga ggaagagctc agatagaaaa ggagggagct actctcaggc
     1141 tgcaagcagt gacagtgccc agggctctga tgtgtctctc acagcttgta aagtgcatca
     1201 ccatcaccat cactgactcg agaagggtgg gcgcgccgac ccagctttct tgtacaaagt
     1261 ggttgatcta gagggcccgc ggttcgaagg taagcctatc cctaaccctc aattcgagca
     1321 tcttaccgcc atttattccc atatttgttc tgtttttctt gatttgggta tacatttaaa
     1381 tgttaataaa acaaaatggt ggggcaatca tttacatttt atgggatatg taattactag
     1441 ttcaggtgta ttgccacaag acaaacatgt taagaaactt tcccgttatt tacgctctgt
     1501 tcctgttaat caacctctgg attacaaaat ttgtgaaaga ttgactgata ttcttaacta
     1561 tgttgctcct tttacgctgt gtggatatgc tgctttaatg cctctgtatc atgctattgc
     1621 ttcccgtacg gctttcgttt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     1681 ggagttgtgg cccgttgtcc gtcaacgtgg cgtggtgtgc tctgtgtttg ctgacgcaac
     1741 ccccactggc tggggcattg ccaccacctg tcaactcctt tctgggactt tcgctttccc
     1801 cctcccgatc gccacggcag aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     1861 taggttgctg ggcactgata attccgtggt gttgtcgggg aagctgacgt cctttccatg
     1921 gctgctcgcc tgtgttgcca actggatcct gcgcgggacg tccttctgct acgtcccttc
     1981 ggctctcaat ccagcggacc tcccttcccg aggccttctg ccggttctgc ggcctctccc
     2041 gcgtcttcgc tttcggcctc cgacgagtcg gatctccctt tgggccgcct ccccgcctgt
     2101 ttcgcctcgg cgtccggtcc gtgttgcttg gtcgtcacct gtgcagaatt gcgaaccatg
     2161 gattccaccg tgaactttgt ctcctggcat gcaaatcgtc aacttggcat gccaagaata
     2221 attcggatcc aagcttaggc ctgctcgctt tcttgctgtc ccatttctat taaaggttcc
     2281 tttgttccct aagtccaact actaaactgg gggatattat gaagggcctt gagcatctgg
     2341 attctgccta gcgctaagct taacacgagc catagataga ataaaagatt ttatttagtc
     2401 tccagaaaaa ggggggaatg aaagacccca cctgtaggtt tggcaagcta gcttaagtaa
     2461 cgccattttg caaggcatgg aaaatacata actgagaata gagaagttca gatcaaggtt
     2521 aggaacagag agacagcaga atatgggcca aacaggatat ctgtggtaag cagttcctgc
     2581 cccggctcag ggccaagaac agttggaaca gcagaatatg ggccaaacag gatatctgtg
     2641 gtaagcagtt cctgccccgg ctcagggcca agaacagatg gtccccagat gcggtcccgc
     2701 cctcagcagt ttctagagaa ccatcagatg tttccagggt gccccaagga cctgaaatga
     2761 ccctgtgcct tatttgaact aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct
     2821 gctccccgag ctcaataaaa gagcccacaa cccctcactc ggcgcgccag tcctccgata
     2881 gactgcgtcg cccgggtacc cgtgttctca ataaaccctc ttgcagttgc atccgactcg
     2941 tggtctcgct gttccttggg agggtctcct ctgagtgatt gactgcccac ctcgggggtc
     3001 tttcattctc gagcagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     3061 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     3121 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     3181 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     3241 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     3301 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     3361 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     3421 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     3481 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt cgccctggaa
     3541 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     3601 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     3661 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     3721 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     3781 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     3841 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     3901 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     3961 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     4021 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     4081 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     4141 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     4201 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     4261 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     4321 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     4381 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     4441 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     4501 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     4561 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     4621 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     4681 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     4741 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     4801 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     4861 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     4921 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     4981 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     5041 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     5101 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     5161 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     5221 ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa
     5281 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     5341 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact
     5401 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
     5461 gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt
     5521 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
     5581 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
     5641 cggccagtga attagtactc tagcttaagt aacgccattt tgcaaggcat ggaaaataca
     5701 taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca gaatatgggc
     5761 caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga acagttggaa
     5821 cagcagaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc ggctcagggc
     5881 caagaacaga tggtccccag atgcggtccc gccctcagca gtttctagag aaccatcaga
     5941 tgtttccagg gtgccccaag gacctgaaat gaccctgtgc cttatttgaa ctaaccaatc
     6001 agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg agctcaataa aagagcccac
     6061 aacccctcac tcggcgcgcc agtcctccga tagactgcgt cgcccgggta cccgtattcc
     6121 caataaagcc tcttgctgtt tgcatccgaa tcgtggactc gctgatcctt gggagggtct
     6181 cctcagattg attgactgcc cacctcgggg gtctttcatt tggaggttcc accgagattt
     6241 ggagacccct gcccagggac caccgacccc cccgccggga ggtaagctgg ccagcggtcg
     6301 tttcgtgtct gtctctgtct ttgtgcgtgt ttgtgccggc atctaatgtt tgcgcctgcg
     6361 tctgtactag ttggctaact agatctgtat ctggcggtcc cgcggaagaa ctgacgagtt
     6421 cgtattcccg gccgcagccc ctgggagacg tcccagcggc ctcgggggcc cgttttgtgg
     6481 cccattctgt atcagttaac ctacccgagt cggacttttt ggagctccgc cactgtccga
     6541 ggggtacgtg gctttgttgg gggacgagag acagagacac ttcccgcccc cgtctgaatt
     6601 tttgctttcg gttttacgcc gaaaccgcgc cgcgcgtctt gtctgctgca gcatcgttct
     6661 gtgttgtctc tgtctgactg tgtttctgta tttgtctgaa aattagctcg acaaagttaa
     6721 gtaatagtcc ctctctccaa gctcacttac aggcggcc
//
