LOCUS       sequence-209540-        5702 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  high transduction efficiency AAV-mediated synapsin 
            promoter-dependent expression IRES-hrGFP .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5702)
  AUTHORS   Pousinha PA, Mouska X, Raymond EF, Gwizdek C, Dhib G, Poupon G, 
            Zaragosi LE, Giudici C, Bethus I, Pacary E, Willem M, Marie H
  TITLE     Physiological and pathophysiological control of synaptic GluN2B-NMDA
            receptors by the C-terminal domain of amyloid precursor protein.
  JOURNAL   Elife. 2017 Jul 6;6. doi: 10.7554/eLife.25659.
  PUBMED    28682239
REFERENCE   2  (bases 1 to 5702)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-07-6"; volume: "6. doi"; pages: " 10.7554/eLife.25659"
COMMENT     Sequence Label: pAAV-syn-IRES-hrGFP
FEATURES             Location/Qualifiers
     source          1..5702
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      178..187
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             184..903
                     /codon_start=1
                     /product="humanized Renilla green fluorescent protein"
                     /label=hrGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKQILKNTGLQEIMSFKVNLEGVVNNHVFTMEGCGKGNILFGN
                     QLVQIRVTKGAPLPFAFDILSPAFQYGNRTFTKYPEDISDFFIQSFPAGFVYERTLRYE
                     DGGLVEIRSDINLIEEMFVYRVEYKGRNFPNDGPVMKKTITGLQPSFEVVYMNDGVLVG
                     QVILVYRLNSGKFYSCHMRTLMKSKGVVKDFPEYHFIQHRLEKTYVEDGGFVEQHETAI
                     AQLTSLGKPLGSLHEWV"
     misc_feature    928..1516
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(981..1001)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1399..1410)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    1536..2012
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(1735..1754)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   2058..2198
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2058..2187
                     /label=AAV2 ITR
     rep_origin      2273..2728
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2360..2379)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2570..2591
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2745..2764)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2864..2886
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2924..2942)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3010..3114
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3115..3183,3184..3975)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3333..3352)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4146..4734
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4635..4654
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   4796..4925
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        5136..5583
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      5596..5605
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat
       61 ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaacgtct
      121 aggccccccg aacgcggagg agtcgtgtcg tgcctgagag cgcagtcgag aggatccgcc
      181 accatggtga gcaagcagat cctgaagaac accggcctgc aggagatcat gagcttcaag
      241 gtgaacctgg agggcgtggt gaacaaccac gtgttcacca tggagggctg cggcaagggc
      301 aacatcctgt tcggcaacca gctggtgcag atccgcgtga ccaagggcgc ccccctgccc
      361 ttcgccttcg acatcctgag ccccgccttc cagtacggca accgcacctt caccaagtac
      421 cccgaggaca tcagcgactt cttcatccag agcttccccg ccggcttcgt gtacgagcgc
      481 accctgcgct acgaggacgg cggcctggtg gagatccgca gcgacatcaa cctgatcgag
      541 gagatgttcg tgtaccgcgt ggagtacaag ggccgcaact tccccaacga cggccccgtg
      601 atgaagaaga ccatcaccgg cctgcagccc agcttcgagg tggtgtacat gaacgacggc
      661 gtgctggtgg gccaggtgat cctggtgtac cgcctgaaca gcggcaagtt ctacagctgc
      721 cacatgcgca ccctgatgaa gagcaagggc gtggtgaagg acttccccga gtaccacttc
      781 atccagcacc gcctggagaa gacctacgtg gaggacggcg gcttcgtgga gcagcacgag
      841 accgccatcg cccagctgac cagcctgggc aagcccctgg gcagcctgca cgagtgggtg
      901 taagaattcc gtacaaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga
      961 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     1021 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     1081 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     1141 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     1201 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     1261 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     1321 aaatcatcgt cctttccttg gctgctcgcc tatgttgcca cctggattct gcgcgggacg
     1381 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     1441 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     1501 tgggccgcct ccccgcatcg ataccggctc gagacgggtg gcatccctgt gacccctccc
     1561 cagtgcctct cctggccctg gaagttgcca ctccagtgcc caccagcctt gtcctaataa
     1621 aattaagttg catcattttg tctgactagg tgtccttcta taatattatg gggtggaggg
     1681 gggtggtatg gagcaagggg caagttggga agacaacctg tagggcctgc ggggtctatt
     1741 gggaaccaag ctggagtgca gtggcacaat cttggctcac tgcaatctcc gcctcctggg
     1801 ttcaagcgat tctcctgcct cagcctcccg agttgttggg attccaggca tgcatgacca
     1861 ggctcagcta atttttgttt ttttggtaga gacggggttt caccatattg gccaggctgg
     1921 tctccaactc ctaatctcag gtgatctacc caccttggcc tcccaaattg ctgggattac
     1981 aggcgtgaac cactgctccc ttccctgtcc ttctcgagct gattttgtag gtaaccacgt
     2041 gcggaccgag cggccgcagg aacccctagt gatggagttg gccactccct ctctgcgcgc
     2101 tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc
     2161 ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc ggtattttct
     2221 ccttacgcat ctgtgcggta tttcacaccg catacgtcaa agcaaccata gtacgcgccc
     2281 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     2341 gccagcgcct tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     2401 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     2461 cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc
     2521 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     2581 ttccaaactg gaacaacact caactctatc tcgggctatt cttttgattt ataagggatt
     2641 ttgccgattt cggtctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     2701 tttaacaaaa tattaacgtt tacaatttta tggtgcactc tcagtacaat ctgctctgat
     2761 gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
     2821 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     2881 cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta
     2941 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     3001 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     3061 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     3121 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     3181 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     3241 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     3301 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     3361 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     3421 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     3481 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     3541 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     3601 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     3661 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     3721 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     3781 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     3841 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     3901 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     3961 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     4021 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     4081 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     4141 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     4201 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     4261 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     4321 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     4381 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     4441 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     4501 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     4561 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     4621 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     4681 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     4741 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgtcctgc
     4801 aggcagctgc gcgctcgctc gctcactgag gccgcccggg cgtcgggcga cctttggtcg
     4861 cccggcctca gtgagcgagc gagcgcgcag agagggagtg gccaactcca tcactagggg
     4921 ttcctgcggc cgcacgcgtt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     4981 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     5041 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg cagcaaatgg
     5101 ttaattaatc tagactgcag agggccctgc gtatgagtgc aagtgggttt taggaccagg
     5161 atgaggcggg gtgggggtgc ctacctgacg accgaccccg acccactgga caagcaccca
     5221 acccccattc cccaaattgc gcatccccta tcagagaggg ggaggggaaa caggatgcgg
     5281 cgaggcgcgt gcgcactgcc agcttcagca ccgcggacag tgccttcgcc cccgcctggc
     5341 ggcgcgcgcc accgccgcct cagcactgaa ggcgcgctga cgtcactcgc cggtcccccg
     5401 caaactcccc ttcccggcca ccttggtcgc gtccgcgccg ccgccggccc agccggaccg
     5461 caccacgcga ggcgcgagat aggggggcac gggcgcgacc atctgcgctg cggcgccggc
     5521 gactcagcgc tgcctcagtc tgcggtgggc agcggaggag tcgtgtcgtg cctgagagcg
     5581 cagtcgagag gatccgccac catggtgagc aagcagatcc tgaagaacac cggcctgcag
     5641 gagatcatga gcttcaaggt gaacctggag ggcgtggtga acaaccacgt gttcaccatg
     5701 gg
//
