LOCUS       sequence-209586-         702 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses full length HPF1 in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Liszczak G, Diehl KL, Dann GP, Muir TW
  TITLE     Acetylation blocks DNA damage-induced chromatin ADP-ribosylation.
  JOURNAL   Nat Chem Biol. 2018 Jul 16. pii: 10.1038/s41589-018-0097-1. doi: 
            10.1038/s41589-018-0097-1.
  PUBMED    30013063
REFERENCE   2  (bases 1 to 702)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2018 Jul 16. pii: 10.1038/s41589-018-0097-1. doi: 
            10.1038/s41589-018-0097-1."
COMMENT     Sequence Label: pET30-HPF1-His-Sumo-Flag
FEATURES             Location/Qualifiers
     source          1..702
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             55..72
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             79..372
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="MSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPL
                     RRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIGG"
     CDS             382..405
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gaaggagata tacatatggg cagcagccat cntnancatc atcacggcag cggcctggtg
       61 ccgcgcggca gcgctagcat gtcggactca gaagtcaatc aagaagctaa gccagaggtc
      121 aagccagaag tcaagcctga gactcacatc aatttaaagg tgtccgatgg atcttcagag
      181 atcttcttca agatcaaaaa gaccactcct ttaagaaggc tgatggaagc gttcgctaaa
      241 agacagggta aggaaatgga ctccttaaga ttcttgtacg acggtattag aattcaagct
      301 gatcagaccc ctgaagattt ggacatggag gataacgata ttattgaggc tcacagagaa
      361 cagattggtg gttcaggtag tgactacaaa gacgatgacg acaagggcgg atctggcatg
      421 gtcggcggtg gcgggaagcg caggcccggc ggggaggggc cgcagtgtga aaaaacaact
      481 gatgtgaaga aaagtaaatt ctgtgaagct gatgtctcca gtgaccttcg aaaagaagta
      541 gaaaatcatt ataagctttc tttacctgaa gatttctatc acttctggaa gttctgtgaa
      601 gaacttgatc ctgaaaagcc atctgattca ctttctgcaa gccttggact tcaattagtt
      661 ggtccttatg atatccttgc tggaaaacat aaaacgaaga aa
//
