LOCUS       sequence-20965-e         674 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 674)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 674)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG414GPD-ccdB-DsRed
FEATURES             Location/Qualifiers
     source          1..674
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(130..254)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(466..486)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
ORIGIN
        1 gtctcgttat cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg
       61 ccattaacct gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtctg
      121 caggtcgacc atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta
      181 tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttc
      241 ttgtacaaag tggtgatggg ctgcaggaat tcgatatcaa gcttaatgga caacaccgag
      301 gacgtcatca aggagttcat gcagttcaag gtgcgcatgg agggctccgt gaacggccac
      361 tacttcgaga tcgagggcga gggcgagggc aagccctacg agggcaccca gaccgccaag
      421 ctgcaggtga ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc cccccagttc
      481 cagtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta catgaagctg
      541 tccttccccg agggcttcac ctgggagcgc tccatgaact tcgaggacgg cggcgtggtg
      601 gaggtgcagc aggactcctc cctgcaggac ggcaccttca tctacaaggt gaagttcaag
      661 ggcgtgaact tccc
//
