LOCUS       sequence-209769-       14556 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Binary vectors used for the inducible expression of tripartite 
            split-sfGFP components (with a 3xHA tag) in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14556)
  AUTHORS   Liu TY, Chou WC, Chen WY, Chu CY, Dai CY, Wu PY
  TITLE     Detection of membrane protein-protein interaction in planta based on
            dual intein-coupled tripartite split-GFP association.
  JOURNAL   Plant J. 2018 Feb 16. doi: 10.1111/tpj.13874.
  PUBMED    29451720
REFERENCE   2  (bases 1 to 14556)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2018 Feb 16. doi: 10.1111/tpj.13874."
COMMENT     Sequence Label: UBQ10:sXVE:S10-(MCS)-3xHA
FEATURES             Location/Qualifiers
     source          1..14556
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      67..778
                     /label=oriV
                     /note="incP origin of replication"
     mobile_element  1400..2167
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     CDS             2359..3153
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     CDS             3452..4600
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    6196..6220
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     complement(6467..6488)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6679..6698
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6823..6845
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6837..6854
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6838..6854
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(6867..7067)
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     CDS             complement(7114..7917)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGAWIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7225..7244)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     7835..7854
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(7967..8146)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        11408..11454
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     CDS             11625..11714
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      11743..11995
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    12163..12187
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             12652..13302
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     primer_bind     complement(13490..13507)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(13741..13760)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     oriT            13983..14092
                     /note="incP origin of transfer"
     CDS             14125..14496
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
ORIGIN
        1 ctccatcaag aagagcgact tcgcggagct ggtgaagtac atcaccgacg agcaaggcaa
       61 gaccgagcgc ctttccgacg ctcaccgggc tggttgccct cgccgctggg ctggcggccg
      121 tctatggccc tgcaaacgcg ccagaaacgc cgtcgaagcc gtgtgcgaga caccgcggcc
      181 gccggcgttg tggatacctc gcggaaaact tggccctcac tgacagatga ggggcggacg
      241 ttgacacttg aggggccgac tcacccggcg cggcgttgac agatgagggg caggctcgat
      301 ttcggccggc gacgtggagc tggccagcct cgcaaatcgg cgaaaacgcc tgattttacg
      361 cgagtttccc acagatgatg tggacaagcc tggggataag tgccctgcgg tattgacact
      421 tgaggggcgc gactactgac agatgagggg cgcgatcctt gacacttgag gggcagagtg
      481 ctgacagatg aggggcgcac ctattgacat ttgaggggct gtccacaggc agaaaatcca
      541 gcatttgcaa gggtttccgc ccgtttttcg gccaccgcta acctgtcttt taacctgctt
      601 ttaaaccaat atttataaac cttgttttta accagggctg cgccctgtgc gcgtgaccgc
      661 gcacgccgaa ggggggtgcc cccccttctc gaaccctccc ggcccgctaa cgcgggcctc
      721 ccatcccccc aggggctgcg cccctcggcc gcgaacggcc tcaccccaaa aatggcagcg
      781 ctggcagtcc ttgccattgc cgggatcggg gcagtaacgg gatgggcgat cagcccgagc
      841 gcgacgcccg gaagcattga cgtgccgcag gtgctggcat cgacattcag cgaccaggtg
      901 ccgggcagtg agggcggcgg cctgggtggc ggcctgccct tcacttcggc cgtcggggca
      961 ttcacggact tcatggcggg gccggcaatt tttaccttgg gcattcttgg catagtggtc
     1021 gcgggtgccg tgctcgtgtt cgggggtgcg ataaacccag cgaaccattt gaggtgatag
     1081 gtaagattat accgaggtat gaaaacgaga attggacctt tacagaatta ctctatgaag
     1141 cgccatattt aaaaagctac caagacgaag aggatgaaga ggatgaggag gcagattgcc
     1201 ttgaatatat tgacaatact gataagataa tatatctttt atatagaaga tatcgccgta
     1261 tgtaaggatt tcagggggca aggcataggc agcgcgctta tcaatatatc tatagaatgg
     1321 gcaaagcata aaaacttgca tggactaatg cttgaaacca ggacaataac cttatagctt
     1381 gtaaattcta tcataattgg gtaatgactc caacttattg atagtgtttt atgttcagat
     1441 aatgcccgat gactttgtca tgcagctcca ccgattttga gaacgacagc gacttccgtc
     1501 ccagccgtgc caggtgctgc ctcagattca ggttatgccg ctcaattcgc tgcgtatatc
     1561 gcttgctgat tacgtgcagc tttcccttca ggcgggattc atacagcggc cagccatccg
     1621 tcatccatat caccacgtca aagggtgaca gcaggctcat aagacgcccc agcgtcgcca
     1681 tagtgcgttc accgaatacg tgcgcaacaa ccgtcttccg gagactgtca tacgcgtaaa
     1741 acagccagcg ctggcgcgat ttagccccga catagcccca ctgttcgtcc atttccgcgc
     1801 agacgatgac gtcactgccc ggctgtatgc gcgaggttac cgactgcggc ctgagttttt
     1861 taagtgacgt aaaatcgtgt tgaggccaac gcccataatg cgggctgttg cccggcatcc
     1921 aacgccattc atggccatat caatgatttt ctggtgcgta ccgggttgag aagcggtgta
     1981 agtgaactgc agttgccatg ttttacggca gtgagagcag agatagcgct gatgtccggc
     2041 ggtgcttttg ccgttacgca ccaccccgtc agtagctgaa caggagggac agctgataga
     2101 aacagaagcc actggagcac ctcaaaaaca ccatcataca ctaaatcagt aagttggcag
     2161 catcacccat aattgtggtt tcaaaatcgg ctccgtcgat actatgttat acgccaactt
     2221 tgaaaacaac tttgaaaaag ctgttttctg gtatttaagg ttttagaatg caaggaacag
     2281 tgaattggag ttcgtcttgt tataattagc ttcttggggt atctttaaat actgtagaaa
     2341 agaggaagga aataataaat ggctaaaatg agaatatcac cggaattgaa aaaactgatc
     2401 gaaaaatacc gctgcgtaaa agatacggaa ggaatgtctc ctgctaaggt atataagctg
     2461 gtgggagaaa atgaaaacct atatttaaaa atgacggaca gccggtataa agggaccacc
     2521 tatgatgtgg aacgggaaaa ggacatgatg ctatggctgg aaggaaagct gcctgttcca
     2581 aaggtcctgc actttgaacg gcatgatggc tggagcaatc tgctcatgag tgaggccgat
     2641 ggcgtccttt gctcggaaga gtatgaagat gaacaaagcc ctgaaaagat tatcgagctg
     2701 tatgcggagt gcatcaggct ctttcactcc atcgacatat cggattgtcc ctatacgaat
     2761 agcttagaca gccgcttagc cgaattggat tacttactga ataacgatct ggccgatgtg
     2821 gattgcgaaa actgggaaga agacactcca tttaaagatc cgcgcgagct gtatgatttt
     2881 ttaaagacgg aaaagcccga agaggaactt gtcttttccc acggcgacct gggagacagc
     2941 aacatctttg tgaaagatgg caaagtaagt ggctttattg atcttgggag aagcggcagg
     3001 gcggacaagt ggtatgacat tgccttctgc gtccggtcga tcagggagga tatcggggaa
     3061 gaacagtatg tcgagctatt ttttgactta ctggggatca agcctgattg ggagaaaata
     3121 aaatattata ttttactgga tgaattgttt tagtacctag atgtggcgca acgatgccgg
     3181 cgacaagcag gagcgcaccg acttcttccg catcaagtgt tttggctctc aggccgaggc
     3241 ccacggcaag tatttgggca aggggtcgct ggtattcgtg cagggcaaga ttcggaatac
     3301 caagtacgag aaggacggcc agacggtcta cgggaccgac ttcattgccg ataaggtgga
     3361 ttatctggac accaaggcac caggcgggtc aaatcaggaa taagggcaca ttgccccggc
     3421 gtgagtcggg gcaatcccgc aaggagggtg aatgaatcgg acgtttgacc ggaaggcata
     3481 caggcaagaa ctgatcgacg cggggttttc cgccgaggat gccgaaacca tcgcaagccg
     3541 caccgtcatg cgtgcgcccc gcgaaacctt ccagtccgtc ggctcgatgg tccagcaagc
     3601 tacggccaag atcgagcgcg acagcgtgca actggctccc cctgccctgc ccgcgccatc
     3661 ggccgccgtg gagcgttcgc gtcgtctcga acaggaggcg gcaggtttgg cgaagtcgat
     3721 gaccatcgac acgcgaggaa ctatgacgac caagaagcga aaaaccgccg gcgaggacct
     3781 ggcaaaacag gtcagcgagg ccaagcaggc cgcgttgctg aaacacacga agcagcagat
     3841 caaggaaatg cagctttcct tgttcgatat tgcgccgtgg ccggacacga tgcgagcgat
     3901 gccaaacgac acggcccgct ctgccctgtt caccacgcgc aacaagaaaa tcccgcgcga
     3961 ggcgctgcaa aacaaggtca ttttccacgt caacaaggac gtgaagatca cctacaccgg
     4021 cgtcgagctg cgggccgacg atgacgaact ggtgtggcag caggtgttgg agtacgcgaa
     4081 gcgcacccct atcggcgagc cgatcacctt cacgttctac gagctttgcc aggacctggg
     4141 ctggtcgatc aatggccggt attacacgaa ggccgaggaa tgcctgtcgc gcctacaggc
     4201 gacggcgatg ggcttcacgt ccgaccgcgt tgggcacctg gaatcggtgt cgctgctgca
     4261 ccgcttccgc gtcctggacc gtggcaagaa aacgtcccgt tgccaggtcc tgatcgacga
     4321 ggaaatcgtc gtgctgtttg ctggcgacca ctacacgaaa ttcatatggg agaagtaccg
     4381 caagctgtcg ccgacggccc gacggatgtt cgactatttc agctcgcacc gggagccgta
     4441 cccgctcaag ctggaaacct tccgcctcat gtgcggatcg gattccaccc gcgtgaagaa
     4501 gtggcgcgag caggtcggcg aagcctgcga agagttgcga ggcagcggcc tggtggaaca
     4561 cgcctgggtc aatgatgacc tggtgcattg caaacgctag ggccttgtgg ggtcagttcc
     4621 ggctgggggt tcagcagcca gcgctttact ggcatttcag gaacaagcgg gcactgctcg
     4681 acgcacttgc ttcgctcagt atcgctcggg acgcacggcg cgctctacga actgccgata
     4741 aacagaggat taaaattgac aattgtgatt aaggctcaga ttcgacggct tggagcggcc
     4801 gacgtgcagg atttccgcga gatccgattg tcggccctga agaaagctcc agagatgttc
     4861 gggtccgttt acgagcacga ggagaaaaag cccatggagg cgttcgctga acggttgcga
     4921 gatgccgtgg cattcggcgc ctacatcgac ggcgagatca ttgggctgtc ggtcttcaaa
     4981 caggaggacg gccccaagga cgctcacaag gcgcatctgt ccggcgtttt cgtggagccc
     5041 gaacagcgag gccgaggggt cgccggtatg ctgctgcggg cgttgccggc gggtttattg
     5101 ctcgtgatga tcgtccgaca gattccaacg ggaatctggt ggatgcgcat cttcatcctc
     5161 ggcgcactta atatttcgct attctggagc ttgttgttta tttcggtcta ccgcctgccg
     5221 ggcggggtcg cggcgacggt aggcgctgtg cagccgctga tggtcgtgtt catctctgcc
     5281 gctctgctag gtagcccgat acgattgatg gcggtcctgg gggctatttg cggaactgcg
     5341 ggcgtggcgc tgttggtgtt gacaccaaac gcagcgctag atcctgtcgg cgtcgcagcg
     5401 ggcctggcgg gggcggtttc catggcgttc ggaaccgtgc tgacccgcaa gtggcaacct
     5461 cccgtgcctc tgctcacctt taccgcctgg caactggcgg ccggaggact tctgctcgtt
     5521 ccagtagctt tagtgtttga tccgccaatc ccgatgccta caggaaccaa tgttctcggc
     5581 ctggcgtggc tcggcctgat cggagcgggt ttaacctact tcctttggtt ccgggggatc
     5641 tcgcgactcg aacctacagt tgtttcctta ctgggctttc tcagccccag atctggggtc
     5701 gatcagccgg ggatgcatca ggccgacagt cggaacttcg ggtccccgac ctgtaccatt
     5761 cggtgagcaa tggatagggg agttgatatc gtcaacgttc acttctaaag aaatagcgcc
     5821 actcagcttc ctcagcggct ttatccagcg atttcctatt atgtcggcat agttctcaag
     5881 atcgacagcc tgtcacggtt aagcgagaaa tgaataagaa ggctgataat tcggatctct
     5941 gcgagggaga tgatatttga tcacaggcag caacgctctg tcatcgttac aatcaacatg
     6001 ctaccctccg cgagatcatc cgtgtttcaa acccggcagc ttagttgccg ttcttccgaa
     6061 tagcatcggt aacatgagca aagtctgccg ccttacaacg gctctcccgc tgacgccgtc
     6121 ccggactgat gggctgcctg tatcgagtgg tgattttgtg ccgagctgcc ggtcggggag
     6181 ctgttggctg gctggtggca ggatatattg tggtgtaaac aaattgacgc ttagacaact
     6241 taataacaca ttgcggacgt ttttaatgta ctggggtggt ttttcttttc accagtgaga
     6301 cgggcaacag ctgattgccc ttcaccgcct ggccctgaga gagttgcagc aagcggtcca
     6361 cgctggtttg ccccagcagg cgaaaatcct gtttgatggt ggttccgaaa tcggcaaaat
     6421 cccttataaa tcaaaagaat agcccgagat agggttgagt gttgttccag tttggaacaa
     6481 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
     6541 cgatggccca ctacgtgaac catcacccaa atcaagtttt ttggggtcga ggtgccgtaa
     6601 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc
     6661 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgccattc aggctgcgca
     6721 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
     6781 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
     6841 aaacgacggc cagtgaatta attcccatct tgaaagaaat atagtttaaa tatttattga
     6901 taaaataaca agtcaggtat tatagtccaa gcaaaaacat aaatttattg atgcaagttt
     6961 aaattcagaa atatttcaat aactgattat atcagctggt acattgccgt agatgaaaga
     7021 ctgagtgcga tattatgtgt aatacataaa ttgatgatat agctagctta gctcatcggg
     7081 ggatcgatcc gtcgaagcta gcttgggtcc cgctcagaag aactcgtcaa gaaggcgata
     7141 gaaggcgatg cgctgcgaat cgggagcggc gataccgtaa agcacgagga agcggtcagc
     7201 ccattcgccg ccaagctctt cagcaatatc acgggtagcc aacgctatgt cctgatagcg
     7261 gtccgccaca cccagccggc cacagtcgat gaatccagaa aagcggccat tttccaccat
     7321 gatattcggc aagcaggcat cgccatgggt cacgacgaga tcctcgccgt cgggcatgcg
     7381 cgccttgagc ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt cgtccagatc
     7441 atcctgatcg acaagaccgg cttccatccg agtacgtgct cgctcgatgc gatgtttcgc
     7501 ttggtggtcg aatgggcagg tagccggatc aagcgtatgc agccgccgca ttgcatcagc
     7561 catgatggat actttctcgg caggagcaag gtgagatgac aggagatcct gccccggcac
     7621 ttcgcccaat agcagccagt cccttcccgc ttcagtgaca acgtcgagca cagctgcgca
     7681 aggaacgccc gtcgtggcca gccacgatag ccgcgctgcc tcgtcctgca gttcattcag
     7741 ggcaccggac aggtcggtct tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa
     7801 cacggcggca tcagagcagc cgattgtctg ttgtgcccag tcatagccga atagcctctc
     7861 cacccaagcg gccggagaac ctgcgtgcaa tccatcttgt tcaatccaag ctcccatggg
     7921 ccctcgacta gagtcgagat ctggattgag agtgaatatg agactctaat tggataccga
     7981 ggggaattta tggaacgtca gtggagcatt tttgacaaga aatatttgct agctgatagt
     8041 gaccttaggc gacttttgaa cgcgcaataa tggtttctga cgtatgtgct tagctcatta
     8101 aactccagaa acccgcggct gagtggctcc ttcaacgttg cggttctgtc agttccaaac
     8161 gtaaaacggc ttgtcccgcg tcatcggcgg gggtcataac gtgactccct taattctccg
     8221 ctcatgatct tgatcccctg cgccatcaga tccttggcgg caagaaagcc atccagttta
     8281 ctttgcaggg cttcccaacc ttaccagagg gcgccccagc tggcaattcc ggttcgcttg
     8341 ctgtccataa aaccgcccag tctagctatc gccatgtaag cccactgcaa gctacctgct
     8401 ttctctttgc gcttgcgttt tcccttgtcc agatagccca gtagctgaca ttcatccggg
     8461 gtcagcaccg tttctgcgga ctggctttct acgtgttccg cttcctttag cagcccttgc
     8521 gccctgagtg cttgcggcag cgtgaagctt cgacgagtca gtaataaacg gcgtcaaagt
     8581 ggttgcagcc ggcacacacg agtcgtgttt atcaactcaa agcacaaata cttttcctca
     8641 acctaaaaat aaggcaatta gccaaaaaca actttgcgtg taaacaacgc tcaatacacg
     8701 tgtcatttta ttattagcta ttgcttcacc gccttagctt tctcgtgacc tagtcgtcct
     8761 cgtcttttct tcttcttctt ctataaaaca atacccaaag agctcttctt cttcacaatt
     8821 cagatttcaa tttctcaaaa tcttaaaaac tttctctcaa ttctctctac cgtgatcaag
     8881 gtaaatttct gtgttcctta ttctctcaaa atcttcgatt ttgttttcgt tcgatcccaa
     8941 tttcgtatat gttctttggt ttagattctg ttaatcttag atcgaagacg attttctggg
     9001 tttgatcgtt agatatcatc ttaattctcg attagggttt catagatatc atccgatttg
     9061 ttcaaataat ttgagttttg tcgaataatt actcttcgat ttgtgatttc tatctagttc
     9121 tggtgttagt ttctagtttg tgcgatcgaa tttgtcgatt aatctgagtt tttctgatta
     9181 acagactagc ccgcggaaaa tgaaggctct tactgctaga caacaagaag tttttgatct
     9241 tattagagat catatttctc aaactggaat gcctcctact agagctgaaa ttgctcaaag
     9301 acttggattt aggtctccta atgctgctga agaacatctt aaggctcttg ctagaaaggg
     9361 agttattgaa attgtttctg gagcttcaag aggaattaga cttcttcaag aagaagaaga
     9421 aggacttcct cttgttggaa gagttgctgc tggagaacct tcttctgctc ctcctactga
     9481 tgtttctctt ggagatgaac ttcatcttga tggagaagat gttgctatgg ctcatgctga
     9541 tgctcttgat gattttgatc ttgatatgct tggagatgga gattctcctg gacctggatt
     9601 tactcctcat gattctgctc cttatggagc acttgatatg gctgattttg aatttgaaca
     9661 aatgtttact gatgctcttg gaattgatga atatggagga gatccttctg ctggagatat
     9721 gagagctgct aatctttggc cttctcctct tatgattaag aggtctaaga agaactctct
     9781 tgctctttct cttactgctg accaaatggt ttctgctctt cttgatgctg aacctcctat
     9841 tctttattct gaatatgatc ctactagacc tttttctgag gcttctatga tgggacttct
     9901 tactaatctt gctgatagag aacttgttca tatgattaat tgggctaaga gagttcctgg
     9961 atttgttgat cttactcttc atgaccaagt tcatcttctt gaatgtgctt ggcttgaaat
    10021 tcttatgatt ggacttgttt ggaggtctat ggaacatcct gttaaacttc tttttgctcc
    10081 taatcttctt cttgatagaa atcaaggaaa gtgtgttgaa ggaatggttg aaatttttga
    10141 tatgcttctt gctacttctt caagatttag aatgatgaat cttcaaggag aagaatttgt
    10201 ttgtcttaag tctattattc ttcttaattc tggagtttat acttttcttt cttctactct
    10261 taagtctctt gaagaaaagg atcatattca tagagttctt gataagatta ctgatactct
    10321 tattcatctt atggctaagg ctggacttac tcttcaacaa caacatcaaa gacttgctca
    10381 acttcttctt attctttctc atattagaca tatgtctaat aagggaatgg aacatcttta
    10441 ttctatgaag tgtaagaatg ttgttcctct ttatgatctt cttcttgaaa tgcttgatgc
    10501 tcatagactt catgctccta cttcaagagg aggagcttct gttgaagaaa ctgaccaatc
    10561 tcatcttgct actgctggat ctacttcttc tcattctctt caaaagtatt atattactgg
    10621 agaagctgaa ggatttcctg ctactgtttg aagatcttga tcaagctccc tggccaattc
    10681 ccagagatgt tagctgaaat catcactaat cagataccaa aatattcaaa tggaaatatc
    10741 aaatagcttc tgtttcatca aaaatgactc gacctaactg agtaagcatg cttgttcgag
    10801 tattatggca ttgggaaaac tgtttttctt gtaccatttg ttgtgcttgt aatttactgt
    10861 gttttttatt cggttttcgc tatcgaactg tgaaatggaa atggatggag aagagttaat
    10921 gaatgatatg gtccttttgt tcattctcaa attaatatta tttgtttttt ctcttatttg
    10981 ttgtgtgttg aatttgaaat tataagagat atgcaaacat tttgttttga gtaaaaatgt
    11041 gtcaaatcgt ggcctctaat gaccgaagtt aatatgagga gtaaaacatc ccaaacgcgg
    11101 ccgcaaccgg tcttgcatcc agctgggctg caggtcgagg ctaaaaaact aatcgcatta
    11161 tcatcccctc gacgtactgt acatataacc actggtttta tatacagcag tactgtacat
    11221 ataaccactg gttttatata cagcagtcga cgtactgtac atataaccac tggttttata
    11281 tacagcagta ctgtacatat aaccactggt tttatataca gcagtcgagg taagattaga
    11341 tatggatatg tatatggata tgtatatggt ggtaatgcca tgtaatatgc tcgactctag
    11401 gatcttcgca agacccttcc tctatataag gaagttcatt tcatttggag aggacacgct
    11461 gaagctagtc gactctagcc aaatctaggg gatccatgga tttaccagac gaccattacc
    11521 tgtcaacaca aactatcctt tcgaaagatc tcaacggtac ggacgttggg tctggcggtg
    11581 gctccggcgc gcccgggtta attaactcga gtctagagtc gacttaccca tacgatgttc
    11641 cagattacgc tggctaccca tacgatgttc cagattacgc tggatcatac ccatacgatg
    11701 ttccagatta cgctactagc taaatcgagc tcgaatttcc ccgatcgttc aaacatttgg
    11761 caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt
    11821 ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga
    11881 tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata
    11941 tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgggga
    12001 attcagatcg gctgagtggc tccttcaacg ttgcggttct gtcagttcca aacgtaaaac
    12061 ggcttgtccc gcgtcatcgg cgggggtcat aacgtgactc ccttaattct ccgctcatga
    12121 tcagattgtc gtttcccgcc ttcagtttaa actatcagtg tttgacagga tatattggcg
    12181 ggtaaaccta agagaaaaga gcgtttatta gaataatcgg atatttaaaa gggcgtgaaa
    12241 aggtttatcc gttcgtccat ttgtatgtgc atgccaacca cagggttccc cagatctggc
    12301 gccggccagc gagacgagca agattggccg ccgcccgaaa cgatccgaca gcgcgcccag
    12361 cacaggtgcg caggcaaatt gcaccaacgc atacagcgcc agcagaatgc catagtgggc
    12421 ggtgacgtcg ttcgagtgaa ccagatcgcg caggaggccc ggcagcaccg gcataatcag
    12481 gccgatgccg acagcgtcga gcgcgacagt gctcagaatt acgatcaggg gtatgttggg
    12541 tttcacgtct ggcctccgga ccagcctccg ctggtccgat tgaacgcgcg gattctttat
    12601 cactgataag ttggtggaca tattatgttt atcagtgata aagtgtcaag catgacaaag
    12661 ttgcagccga atacagtgat ccgtgccgcc ctggacctgt tgaacgaggt cggcgtagac
    12721 ggtctgacga cacgcaaact ggcggaacgg ttgggggttc agcagccggc gctttactgg
    12781 cacttcagga acaagcgggc gctgctcgac gcactggccg aagccatgct ggcggagaat
    12841 catacgcatt cggtgccgag agccgacgac gactggcgct catttctgat cgggaatgcc
    12901 cgcagcttca ggcaggcgct gctcgcctac cgcgatggcg cgcgcatcca tgccggcacg
    12961 cgaccgggcg caccgcagat ggaaacggcc gacgcgcagc ttcgcttcct ctgcgaggcg
    13021 ggtttttcgg ccggggacgc cgtcaatgcg ctgatgacaa tcagctactt cactgttggg
    13081 gccgtgcttg aggagcaggc cggcgacagc gatgccggcg agcgcggcgg caccgttgaa
    13141 caggctccgc tctcgccgct gttgcgggcc gcgatagacg ccttcgacga agccggtccg
    13201 gacgcagcgt tcgagcaggg actcgcggtg attgtcgatg gattggcgaa aaggaggctc
    13261 gttgtcagga acgttgaagg accgagaaag ggtgacgatt gatcaggacc gctgccggag
    13321 cgcaacccac tcactacagc agagccatgt agacaacatc ccctccccct ttccaccgcg
    13381 tcagacgccc gtagcagccc gctacgggct ttttcatgcc ctgccctagc gtccaagcct
    13441 cacggccgcg ctcggcctct ctggcggcct tctggcgctc ttccgcttcc tcgctcactg
    13501 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
    13561 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
    13621 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
    13681 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
    13741 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
    13801 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt ttccgctgca
    13861 taaccctgct tcggggtcat tatagcgatt ttttcggtat atccatcctt tttcgcacga
    13921 tatacaggat tttgccaaag ggttcgtgta gactttcctt ggtgtatcca acggcgtcag
    13981 ccgggcagga taggtgaagt aggcccaccc gcgagcgggt gttccttctt cactgtccct
    14041 tattcgcacc tggcggtgct caacgggaat cctgctctgc gaggctggcc ggctaccgcc
    14101 ggcgtaacag atgagggcaa gcggatggct gatgaaacca agccaaccag gaagggcagc
    14161 ccacctatca aggtgtactg ccttccagac gaacgaagag cgattgagga aaaggcggcg
    14221 gcggccggca tgagcctgtc ggcctacctg ctggccgtcg gccagggcta caaaatcacg
    14281 ggcgtcgtgg actatgagca cgtccgcgag ctggcccgca tcaatggcga cctgggccgc
    14341 ctgggcggcc tgctgaaact ctggctcacc gacgacccgc gcacggcgcg gttcggtgat
    14401 gccacgatcc tcgccctgct ggcgaagatc gaagagaagc aggacgagct tggcaaggtc
    14461 atgatgggcg tggtccgccc gagggcagag ccatgacttt tttagccgct aaaacggccg
    14521 gggggtgcgc gtgattgcca agcacgtccc catgcg
//
