LOCUS       sequence-209912-         774 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses dCas9-PmCDA1-UGI and gRNA(HPRT) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Nishida K, Arazoe T, Yachie N, Banno S, Kakimoto M, Tabata M, 
            Mochizuki M, Miyabe A, Araki M, Hara KY, Shimatani Z, Kondo A
  TITLE     Targeted nucleotide editing using hybrid prokaryotic and vertebrate 
            adaptive immune systems.
  JOURNAL   Science. 2016 Aug 4. pii: aaf8729.
  PUBMED    27492474
REFERENCE   2  (bases 1 to 774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2016 Aug 4. pii: aaf8729."
COMMENT     Sequence Label: pcDNA3.1_pCMV-dCas-PmCDA1-ugi pH1-gRNA(HPRT)
FEATURES             Location/Qualifiers
     source          1..774
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(33..86)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(93..113)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(747..770)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tgcgtgcatc catcttgttc aatcattcta gatggaccag ggttttcttc cacatcccca
       61 caagtcagaa ggcttcccct gccttcggta ccgacttttc ttttcttctt gggaccaacg
      121 gctggagact tagtggtatg cagaatcttg acctggatca tgatgctcag ctcggatctc
      181 cacttctccg cacgtttcag tgttttctcc aaccagcggt tttcattgag ctggttatga
      241 gagctttgga tgaaaatctt ccgacaacac tgatagtgct cggaaaccat cacgttcagg
      301 cctacaccat tgtccctcag attccagagt cctatctggt tccgggcatt cttctcgtag
      361 tacagcttgc aggcccagat tttcaatgtg tgcccgtttc cccgaagttc ctgattatac
      421 cattccagaa ttttctcggc acaatcagcg caaggactcc agctggaata ccagttgatc
      481 gtaaactgcc caggattatc ccgaagatac tcttccacct ttcggatact gaatatctca
      541 gcgtgtattc ctctctcggt gccactttgg ggcttgttga cggcatagcc ccaaaagcaa
      601 gcccttcttt cgccccttcg cttcaactca aacagcacgt agcagcggtg tgacacagat
      661 ttcttgttgt tgaagaactg cttcttgaag gtgtaaatat ccagtttctc atgaatgcgc
      721 acgtactcgg cgtctgtcat ccctgattta tcgtcgtcgt ctttgtagtc gatg
//
