LOCUS       sequence-209913-       10456 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Used to replace C. glabrata YAP1 promoter with the stronger TDH3 
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10456)
  AUTHORS   Vu BG, Moye-Rowley WS
  TITLE     Construction and Use of a Recyclable Marker To Examine the Role of 
            Major Facilitator Superfamily Protein Members in Candida glabrata 
            Drug Resistance Phenotypes.
  JOURNAL   mSphere. 2018 Mar 28;3(2). pii: mSphere00099-18. doi: 
            10.1128/mSphere.00099-18. eCollection 2018 Mar-Apr.
  PUBMED    29600281
REFERENCE   2  (bases 1 to 10456)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mSphere."; 
            date: "2018-03-28"; volume: "3(2). pii: mSphere00099-18. doi"; 
            pages: " 10.1128/mSphere.00099-18. eCollection 2018 Mar-Apr"
COMMENT     Sequence Label: pBV163
FEATURES             Location/Qualifiers
     source          1..10456
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(12..29)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(183..771)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(263..282)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(942..1733,1734..1802))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1565..1584
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1803..1907)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1975..1993
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2031..2053)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2153..2172
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2366..2388
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2380..2397
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2381..2397
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2998..3031
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        3086..3429
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     terminator      4008..4205
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             7207..8142
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAVNLWPSPLMIKRSKENSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPGKLLFAPNLLLDRNQGKCVEGMVEIFDMLLVTSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLLLSHIRHMSNEGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     terminator      8319..8566
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(8335..8353)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     protein_bind    8592..8625
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(10237..10253)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10237..10253)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10250..10272)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10261..10277
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10285..10291,10292..10309,10310..10315))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10330..10351
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
       61 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
      121 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
      181 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
      241 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
      301 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
      361 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
      421 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
      481 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
      541 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
      601 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
      661 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
      721 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
      781 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
      841 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
      901 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
      961 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     1021 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     1081 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     1141 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     1201 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     1261 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     1321 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     1381 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     1441 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     1501 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     1561 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     1621 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     1681 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     1741 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     1801 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     1861 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     1921 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
     1981 cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     2041 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
     2101 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca
     2161 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
     2221 agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
     2281 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
     2341 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa
     2401 ttcgagctcg gtacctccca tttagaaaga gtttgtgtct gttgtacctg tacttccccg
     2461 tacagccccg tacagccccg tacagccccg tgtgttggtc tttctccggt ttgtattttg
     2521 atttccgttg tgtactgttt tgtactgctg cttctgtagc caaatggaac ccgaaacttt
     2581 gtgtagaagg gtgaaaaagg ccttggcaca gaacatagcg gagtgggaag gtaccccttg
     2641 ttcctgcagc accgtctgtg tcacgacagc acagtgtcct ttcccgttac tgaaagaggt
     2701 gctctccagt tgcgagtgcg gtaatttctt tgggcgatac cgcatcggtg aaggacaggt
     2761 gggaactggg aggggctctt ccctctctct atacacgtaa aactgtgtgt ggggggatga
     2821 tgggcagatt tcaccattat ataagaaaaa cccaatatgc aacagtaact acttgataag
     2881 cgcttacttt tcgaaaccgc acgcaatacc aaataccaga agagcctaca acttgaaggt
     2941 ttcttagaca cgccaaggac ctgaagcttc gtacgctgca ggtcgacaac ccttaatata
     3001 acttcgtata atgtatgcta tacgaagtta ttaggtctag agatctgttt agcttgcctc
     3061 gtccccgccg ggtcacccgg ccagcgacat ggaggcccag aataccctcc ttgacagtct
     3121 tgacgtgcgc agctcagggg catgatgtga ctgtcgcccg tacatttagc ccatacatcc
     3181 ccatgtataa tcatttgcat ccatacattt tgatggccgc acggcgcgaa gcaaaaatta
     3241 cggctcctcg ctgcagacct gcgagcaggg aaacgctccc ctcacagacg cgttgaattg
     3301 tccccacgcc gcgcccctgt agagaaatat aaaaggttag gatttgccac tgaggttctt
     3361 ctttcatata cttcctttta aaatcttgct aggatacagt tctcacatca catccgaaca
     3421 taaacaacca tgggtacaac cttagacgat acagcataca gatacagaac aagtgtccct
     3481 ggtgacgctg aagccattga agccttagac ggtagtttta ctacagacac tgtattcaga
     3541 gttaccgcaa ctggtgatgg ttttactttg agagaagttc cagtcgatcc acctttaaca
     3601 aaagttttcc ctgatgacga atccgatgac gaaagtgatg acggtgaaga tggtgaccca
     3661 gattcaagaa catttgttgc ttatggtgat gacggtgatt tggccggttt cgttgtcgta
     3721 tcttactcag gttggaacag aagattaacc gttgaagata tagaagtcgc tccagaacat
     3781 agaggtcacg gtgttggtag agcattgatg ggtttagcca ccgaatttgc tagagaaaga
     3841 ggtgctggtc atttgtggtt agaagtcact aatgtaaacg ctcctgcaat acacgcctat
     3901 agaagaatgg gtttcacatt gtgtggttta gataccgctt tgtacgacgg tacagcctcc
     3961 gacggtgaac aagccttata catgtctatg ccatgccctt aatcagtact gacaataaaa
     4021 agattcttgt tttcaagaac ttgtcatttg tatagttttt ttatattgta gttgttctat
     4081 tttaatcaaa tgttagcgtg atttatattt tttttcgcct cgacatcatc tgcccagatg
     4141 cgaagttaag tgcgcagaaa gtaatatcat gcgtcaatcg tatgtgaatg ctggtcgcta
     4201 tactgctgtc gattcgatac taacgccgcc atccagtttt ttatattatt tggtctaaca
     4261 taccagttac aattagtatt acaatggttt accaagtaca aattatgtaa tatggttata
     4321 attggcttat atgtatggag gtagggaact gcaggttatt gttctgttaa actaccagta
     4381 atgcaagacg gctaagtcat atgactgcca ctttgtcaat cctgaagaaa atgacaatag
     4441 aaagtgatat aatacacgtg atatgcgtag gatgggaccc aaagatagcc acatcacatg
     4501 atcttcaaaa accccgtcaa aaaggaataa atcgaaggaa aaaaaatgcc acgtgacttt
     4561 gatagtcaaa atcaggttat attactatac gatcccccac acataacctt taccacacac
     4621 cgcacgggct ggacttatcc tattagaaag cggtgcagcc agggccaaga aaacgcgaac
     4681 gacgcagaaa aaacgtcaag caaaaaaact gtggtgttat ttaaagcata caatttcctg
     4741 cctcccttta atgctacggg ggatctagca aatgggaaaa tatcacatga ctttggctag
     4801 aaggggtggg aaaacagata ttttagtcac attgtttgat ttcacgtact acacgataca
     4861 ctacactaca caatacactg caatacacta cactgtatgg ttccctccgg ccctggatat
     4921 gctagcgaag gatcccaagc ccatcgagga aatcattcaa gagcatctgc aggtctcaaa
     4981 tacactaagt ccaaacacaa acagcactga gctaatccaa gacaccaatt gcatgccttc
     5041 tcaattagaa cagtttatta cacagcttat aactgtgcat cttgccattc ttttcgagat
     5101 agccatttgc atttgattaa catgcttagc tcgttctcaa gccacagtaa aatggattgc
     5161 cttttgagtt tccatcgttg tataaatagg cactcattcc tatagccttg ctctttggtc
     5221 ttgtctctgt aatggtaata gctgtaagtc aggatataac actccagaaa agaaacacct
     5281 aacaatatgt ccaatttact gaccggtatg ttcgactact atggatagac aagactataa
     5341 ctcctataat atagggcgtg tggtcgctga taaacaattt cttctacaca caacaaggag
     5401 ttacccagtg ttgactagag tatggccata atctagtcaa atactctaga gatgactctc
     5461 gctatttcat tcaattgcat taatactaac aatttctatt ttaaaatttt aaactacttt
     5521 ctactgcatt gaacttcatt ttggtttttt ctacggtttt tcttttgatc tccttttctt
     5581 atctgtccct gcgtgtgtga ataaccgctg cgatactcaa ttgctttcat aaccattttc
     5641 acaggatcaa ttgctgagac tatcctctca cctctctatc gtttccccct ttgccacatt
     5701 agcctatggc acgtacattt ttttttcacc ctgtgcgaag cgacaatttt ttttttggga
     5761 aaatcatgat aggcgcgtgc gtgggtttct aagggtgacg gcgattatga gttaggagtg
     5821 gtgggcattg ggatcataca tataatgtaa aagtggcaac ggttgatgaa caggcacaac
     5881 tacctgcaca gatgctacta tggcctaggg ctttgcgttt ctctacttat tgagatgctt
     5941 ttgttttagg tgagaaatcg aaagccatgg ctacaaaata ggatctacct ctacaacata
     6001 tgtagtccac ttccatcagt tattggccgg taatggtgtt cacacattgc aattaggtaa
     6061 atgaaagtct tcccccagca gaaggtaaga tcaacattag gagaagacca aagtaaagaa
     6121 gaccaaatga ttatagttca gggataaaga agtggttttt ttttaaaaac cagtatatgt
     6181 acagtacacc aaaatttgcc tgcattaccg gtcgatgcaa cgagtgatga ggttcgcaag
     6241 aacctgatgg acatgttcag ggatcgccag gcgttttctg agcatacctg gaaaatgctt
     6301 ctgtccgttt gccggtcgtg ggcggcatgg tgcaagttga ataaccggaa atggtttccc
     6361 gcagaacctg aagatgttcg cgattatctt ctatatcttc aggcgcgcgg tctggcagta
     6421 aaaactatcc agcaacattt gggccagcta aacatgcttc atcgtcggtc cgggctgcca
     6481 cgaccaagtg acagcaatgc tgtttcactg gttatgcggc ggatccgaaa agaaaacgtt
     6541 gatgccggtg aacgtgcaaa acaggctcta gcgttcgaac gcactgattt cgaccaggtt
     6601 cgttcactca tggaaaatag cgatcgctgc caggatatac gtaatctggc atttctgggg
     6661 attgcttata acaccctgtt acgtatagcc gaaattgcca ggatcagggt taaagatatc
     6721 tcacgtactg acggtgggag aatgttaatc catattggca gaacgaaaac gctggttagc
     6781 accgcaggtg tagagaaggc acttagcctg ggggtaacta aactggtcga gcgatggatt
     6841 tccgtctctg gtgtagctga tgatccgaat aactacctgt tttgccgggt cagaaaaaat
     6901 ggtgttgccg cgccatctgc caccagccag ctatcaactc gcgccctgga agggattttt
     6961 gaagcagctc atcgactgat ttacggcgct gaggatgact ctggtcagag gtacctggcc
     7021 tggtctggac acagtgcccg tgtcggagcc gcgcgagata tggcccgcgc tggagtttca
     7081 ataccggaga tcatgcaagc tggtggctgg accaatgtaa atattgtcat gaactatatc
     7141 cgtaacctgg atagtgaaac aggggcaatg gtggtcctgc tggaagatgg cgatctcgag
     7201 ccatccgctg gagacatgag agctgtcaac ctttggccaa gcccgctcat gatcaaacgc
     7261 tctaaggaga acagcctggc cttgtccctg acggccgacc agatggtcag tgccttgttg
     7321 gatgctgagc cccccatact ctattccgag tatgatccta ccagaccctt cagtgaagct
     7381 tcgatgatgg gcttactgac caacctggca gacagggagc tggttcacat gatcaactgg
     7441 gcgaagaggg tgccaggctt tgtggatttg accctccatg atcaggtcca ccttctagaa
     7501 tgtgcctggc tagagatcct gatgattggt ctcgtctggc gctccatgga acacccgggg
     7561 aagctcctgt ttgctcctaa cttgctcctg gacaggaatc aaggtaaatg tgtggaaggc
     7621 atggtggaga tcttcgacat gctgctggtt acatcatctc ggttccgcat gatgaatctg
     7681 cagggagagg agtttgtgtg cctcaaatct attattttgc ttaattctgg agtatacaca
     7741 tttctgtcca gcaccctgaa gtctctcgag gagaaggacc atatccaccg agtcctggac
     7801 aagatcacag acactttgat ccacctgatg gccaaggcag gcctgaccct gcagcagcag
     7861 caccagcggc tagcccagct cctcctcctc ctctcccaca tcaggcacat gagtaacgaa
     7921 ggcatggagc atctgtacag catgaagtgc aagaacgtgg tacccctcta tgacctgctg
     7981 ctggagatgc tagacgccca ccgcctacat gcgcccacta gtcgtggagg ggcatccgtg
     8041 gaggagacgg accaaagcca cttggccact gcgggctcta cttcatcgca ttccttgcaa
     8101 aagtattaca tcacggggga ggcagagggt ttccctgcca cagtctgagt agaatgcctg
     8161 atattgactc aatatccgtt gcgtttcctg tcaaaagtat gcatagtgct gaacatttcg
     8221 tgatgaatgc caccgaggaa gaagcacggc gcggttttgc taaagtgatg tctgagtttg
     8281 gcgaactctt gggtaaggtt ggaattgtcg acctcgagtc atgtaattag ttatgtcacg
     8341 cttacattca cgccctcccc ccacatccgc tctaaccgaa aaggaaggag ttagacaacc
     8401 tgaagtctag gtccctattt atttttttat agttatgtta gtattaagaa cgttatttat
     8461 atttcaaatt tttctttttt ttctgtacag acgcgtgtac gcatgtaaca ttatactgaa
     8521 aaccttgctt gagaaggttt tgggacgctc gaaggcttta atttgcaaac gagctctcga
     8581 gaacccttaa tataacttcg tataatgtat gctatacgaa gttattaggt gatatcagat
     8641 ccactagtgg cctatgcggc cgccgtcagg tgatcatatc actcacaata tcactcacat
     8701 atcttccaat attacgtagt aactgtgcca ccaataaatg tatgggtgtt aagtatgtat
     8761 tctctgtggg tcccgagaga atgccccgtg tgcagcactg cagcacgtag agatgtacgg
     8821 gggggggaaa ggttttcggg gttccacttt tagtggaacc ccagtgaaga gagaaggacc
     8881 aaagaagaga agagaaggga aggatgttta cgtcatgcag ggattgtccg tctcccgaga
     8941 accgtcgtgg atcggggggg ggtgggtgag agagagagac ctacaaagga ccaaacagga
     9001 atcccctttg tatctgtgga ctcgagagac aatgggaagg gaaagaaagg ggagccccac
     9061 tgcaagacga gacaaagcac cacggcacgt ccttttcaaa cagagttccc agaagtagag
     9121 ggaaaaagag gaatccacca caccaacacc cgaccaccac accaaggacc aaaggaaaga
     9181 aaaagtaacg agacccatcc cccctagagc aatacccaaa cttgttcata ctacgcacag
     9241 tactgccata ccacactacc acactacaaa actgccacat taccacacta ccacactaca
     9301 ctatactaag cccaatgtct tgtgcatagt gtgaggacat tgcatagtgt gagacaagtg
     9361 ttgaagtgtt taaagtgcta aagtggtaag tgctaatgtg gtaagtagta aatggtagtg
     9421 gtgcttttga tggttccagt acaatagaat atcgaagaac aattgggaga ctaccttctt
     9481 ggtgctgaaa tttttttctt gcttcccccc ccgattccaa aaaagatata taaagggaca
     9541 attgtaatag ttaattgcaa tctgaatatt catctttcat ttcttctctt ttatcaattc
     9601 tcctctatat catcaaggaa ttaaaacacc tacaaatcac aacaaacata acatatggct
     9661 gaggtggata acggtggcgc acagaagagc agcgcttcga ggaagaagcg gtaccaggag
     9721 ctggatcctg agacgcgtat gaagcgtgtg gcacagaaca gggctgcgca gaaggcgttc
     9781 agggagcgta aggagcgcaa gatgaaggaa ctggagcgga aagtggtgga cttggagaac
     9841 ttgacaaagc tgaatgaggt ggagaccaac ttcctcaggg accagctgag tatcctggtg
     9901 aaagagttgc ggaagtacag accagagacg aaacaggacc ataaggtatt gaagtacttg
     9961 gagaagcaca agggtggggc tgctggtgct gggaacggtg ccgccaccgg ctcggtttcc
    10021 acatcgacaa gacacacaga tctcgcggcg tcaaatgcta acagagtatc aaaggattct
    10081 agcatcttac ctggtgcgaa gattatacgg caagatcttg agagtttcaa tgaaaatagg
    10141 catttcaacg tcacaggtca gttgacgcca cctggtaaca cgtcctcatc aacaacagca
    10201 aactccctgc aggcatgcaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
    10261 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
    10321 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
    10381 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
    10441 tttgcgtatt gggcgc
//
