LOCUS       sequence-20994-e         582 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 582)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG425GPD-ccdB-DsRed
FEATURES             Location/Qualifiers
     source          1..582
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(116..240)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(452..472)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
ORIGIN
        1 ggagaagtgg ctgatctcag ccaccgcgaa aatgacatca aaaacgccat taacctgatg
       61 ttctggggaa tataaatgtc aggctccctt atacacagcc agtctgcagg tcgaccatag
      121 tgactggata tgttgtgttt tacagtatta tgtagtctgt tttttatgca aaatctaatt
      181 taatatattg atatttatat cattttacgt ttctcgttca gctttcttgt acaaagtggt
      241 gatgggctgc aggaattcga tatcaagctt aatggacaac accgaggacg tcatcaagga
      301 gttcatgcag ttcaaggtgc gcatggaggg ctccgtgaac ggccactact tcgagatcga
      361 gggcgagggc gagggcaagc cctacgaggg cacccagacc gccaagctgc aggtgaccaa
      421 gggcggcccc ctgcccttcg cctgggacat cctgtccccc cagttccagt acggctccaa
      481 ggcctacgtg aagcaccccg ccgacatccc cgactacatg aagctgtcct tccccgaggg
      541 cttcacctgg gaacgctcca tgaacttcga ggacggcggc gt
//
