LOCUS       sequence-209974-       11971 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For Tol2 transposon-mediated stable expression of LRL and 
            IRES-luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11971)
  AUTHORS   Kawauchi D, Ogg RJ, Liu L, Shih DJH, Finkelstein D, Murphy BL, Rehg 
            JE, Korshunov A, Calabrese C, Zindy F, Phoenix T, Kawaguchi Y, 
            Gronych J, Gilbertson RJ, Lichter P, Gajjar A, Kool M, Northcott PA,
            Pfister SM, Roussel MF
  TITLE     Novel MYC-driven medulloblastoma models from multiple embryonic 
            cerebellar cells.
  JOURNAL   Oncogene. 2017 Sep 14;36(37):5231-5242. doi: 10.1038/onc.2017.110. 
            Epub 2017 May 15.
  PUBMED    28504719
REFERENCE   2  (bases 1 to 11971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/onc.2017.110"; journalName: "Oncogene"; date: "2017-09-14- 
            14"; volume: "36"; issue: "37"; pages: "5231-5242"
COMMENT     Sequence Label: pT2K-LRL-IRES-Luc2
FEATURES             Location/Qualifiers
     source          1..11971
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(34..462)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     356..375
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     589..611
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     603..620
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     604..620
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     630..649
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        630..648
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(2499..2510,2511..2673)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     enhancer        3046..3425
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3427..3703
                     /label=chicken beta-actin promoter
     intron          3704..4724
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     4644..4666
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     4732..4751
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    complement(4797..4830)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             4847..5524
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(5027..5047)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     5439..5462
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    5597..5652
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6013..6029)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6037..6053
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6061..6067,6068..6085,6086..6091))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6106..6127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6186..6381
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6232..6367
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    complement(6390..6423)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    6471..7020
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6615..6632)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6842..6861
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7023..8675
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    8778..8833
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     misc_feature    join(9501..9688,9689..9700)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(9734..9750)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9734..9750)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(9787..9807)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9787..9805)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9826..9842)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9850..9866
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9874..9880,9881..9898,9899..9904))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9919..9940
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10057..10074)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10228..10816)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10308..10327)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10987..11778,11779..11847))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11610..11629
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11848..11952)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      241 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      301 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
      361 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
      421 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc
      481 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
      541 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
      601 gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc gaattgggta
      661 ccaaatagta ggaattaccc acctgtacaa gtgctgaaaa cttggatgaa taagcccgtt
      721 tgccattttc tactgctatt ttaaatcttt tctgtatttg tctgcatttg tctttaccct
      781 tgaataaatg ttttagttgt tttttttcaa ttatgactgt gtttaacaga cattattaca
      841 ataatatgta atagtagtac acactattat tatgtaatag tacttgttga ctgtatttga
      901 gaactggacc gagtgagtgt tacgtcaccc attcaaaatg acttacttct ggctccaaca
      961 aaatgaagtt aattcagttg ccatttttca ctgtatggac atcgccgtgt tggagctaga
     1021 cgttgccaat aagcaagaaa gagccgagat gcgtcgagtc tgagtcaccg ttcctatggc
     1081 aacccctcta accaatcaga agtaagcttg ttggaagtcc acagcctacc acttgaaagc
     1141 gggctgcaca aaatctgtca aacgttttga acgttggatg tgagagcaca tacttttatt
     1201 aaggcatctg gttggtcagt ttataatatc aacaacttgg gctacagaaa agaaaagtta
     1261 ttacagaaat tatgattaac aagtacatgt taaataaaga ttttaatatg aatgccacca
     1321 ctggagcatt catgccattt ggagcttctt cctgtttgga tcactagaag gaggaggtca
     1381 ctcattacag ttctcatata cagtcgttgg ttggttggtt ggttggtaga ttgattgatt
     1441 gattgattga ttgattgatt gattgattga ttgattgatt gattgattga ttgattgatt
     1501 gattggtagt caaaataaga aataatttcc acagattcat tacagaaatg attaaatgca
     1561 tacataaaaa actggggggg ggggggatac aacaacacac ttaagtcaca tttgcctacg
     1621 taaagaaaag taaagaaaat caatagctat attttacatc tccttttttt gctgtctttt
     1681 aattagcctt gttttgctgt tatcttgatt gaaactgtaa cttcttcacc tgcttctttt
     1741 ctttgtaggt tttgccagcc cagaaacagg catggctgtg caaggccaga gcaccatgca
     1801 caatgcgctg catgtcttca tgaacggctc aatgtcctca gtccagggct cagccaacga
     1861 ccccatcttc ctccttcacc atgctttcat tgacaggtaa caaacacgtc atgacattag
     1921 actgcacagt ttttgacaaa gttcatacaa tctgttgttt atagctgcta caattagtga
     1981 agtttgtgaa tgtacttgga tgagcagcga aagatcaatt gagatcaatt gttagagttt
     2041 ggttgccctg cagagcaaag aacaaaaaat aatctggtgg ctttactgcg tgaggttatt
     2101 attggtggaa tagaaacaca aaacataatt gcatttattt gtttaatttt ttatcttatc
     2161 ttaactttca tcttgcatat ttgtttctta catcatttct agcatctttg agcgctggct
     2221 aagaactcat cagcctcccc ggtccatcta cccacgtacc aatgcaccaa ttggccacaa
     2281 tgacggctac tacatggtgc cattccttcc tctttatagg aatggagact acctcctgtc
     2341 caacaatgct cttggatacg agtacgccta cctgttggac ccaggtcatt gcacaacacc
     2401 agaaatgccc tctgatctgc aaaagacgtg aatatctgtt cagacaccca tatccactct
     2461 gttccacaca ggtcagaggt ttgtccagga gttcttgaca gaggtgtaaa aagtactcaa
     2521 aaattttact caagtgaaag tacaagtact tagggaaaat tttactcaat taaaagtaaa
     2581 agtatctggc tagaatctta cttgagtaaa agtaaaaaag tactccatta aaattgtact
     2641 tgagtattaa ggaagtaaaa gtaaaagcaa gaaagaaaac tagagattct tgtttaagct
     2701 tttaatctca aaaaacatta aatgaaatgc atacaaggtt ttatcctgct ttagaactgt
     2761 ttgtatttaa ttatcaaact ataagacaga caatctaatg ccagtacacg ctactcaaag
     2821 ttgtaaaacc tcagatttaa cttcagtaga agctgattct caaaattgtt agtgtcaagc
     2881 ctagctcttt tggggctgaa aagcaatcct gcagtgctga aaagcctctc acaggcagcc
     2941 gatgcgggaa gaggtgtatt agtcttgata gagaggctgc aaatagcagg aaacgtgagc
     3001 agagactccc tggtgtctga aacacaggcc agatgggccc tcgtcgacat tgattattga
     3061 ctagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
     3121 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
     3181 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
     3241 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
     3301 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
     3361 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
     3421 ccatggtcga ggtgagcccc acgttctgct tcactctccc catctccccc ccctccccac
     3481 ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg gcgggggggg
     3541 ggggggncgc gcgccrggcg gggcggggsg gggsgrgggg sggggsgggg cgrggcggag
     3601 aggtgcggcg gcagccaatc agagcggcgc gctccgaaag tttcctttta tggcgaggcg
     3661 gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgcgctg
     3721 ccttcgcccc gtgccccgct ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac
     3781 cgcgttactc ccacaggtga gcgggcggga cggcccttct cctccgggct gtaattagcg
     3841 cttggtttaa tgacggcttg tttcttttct gtggctgcgt gaaagccttg aggggctccg
     3901 ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg tgcgtgtgtg tgtgcgtggg
     3961 gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag cgctgcgggc gcggcgcggg
     4021 gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc gggggcggtg ccccgcggtg
     4081 cggggggggc tgcgagggga acaaaggctg cgtgcggggt gtgtgcgtgg gggggtgagc
     4141 agggggtgtg ggcgcgtcgg tcgggctgca accccccctg cacccccctc cccgagttgc
     4201 tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg cgtggcgcgg ggctcgccgt
     4261 gccgggcggg gggtggcggc aggtgggggt gccgggcggg gcggggccgc ctcgggccgg
     4321 ggrgggctcg ggggaggggc gcggcggccc ccggagcgcc ggcggctgtc gaggcgcggc
     4381 gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac ttcctttgtc
     4441 ccaaatctgt gcggagccga aatctgggag gcgccgccgc accccctcta gcgggcgcgg
     4501 ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg gagggccttc gtgcgtcgcc
     4561 gcgccgccgt ccccttctcc ctctccagcc tcggggctgt ccgcgggggg acggctgcct
     4621 tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg gctctagcta
     4681 gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc
     4741 tggttattgt gctgtctcat cattttggca aagaattcct cgagtctaga tcgcgtataa
     4801 cttcgtatag catacattat acgaagttat ctcgagtcta ggatccatgg cctcctccga
     4861 gaacgtcatc accgagttca tgcgcttcaa ggtgcgcatg gagggcaccg tgaacggcca
     4921 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggccaca acaccgtgaa
     4981 gctgaaggtg accaagggcg gccccctgcc cttcgcctgg gacatcctgt ccccccagtt
     5041 ccagtacggc tccaaggtgt acgtgaagca ccccgccgac atccccgact acaagaagct
     5101 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggc
     5161 gaccgtgacc caggactcct ccctgcagga cggctgcttc atctacaagg tgaagttcat
     5221 cggcgtgaac ttcccctccg acggccccgt gatgcagaag aagaccatgg gctgggaggc
     5281 ctccaccgag cgcctgtacc cccgcgacgg cgtgctgaag ggcgagaccc acaaggccct
     5341 gaagctgaag gacggcggcc actacctggt ggagttcaag tccatctaca tggccaagaa
     5401 gcccgtgcag ctgcccggct actactacgt ggacgccaag ctggacatca cctcccacaa
     5461 cgaggactac accatcgtgg agcagtacga gcgcaccgag ggccgccacc acctgttcct
     5521 gtaggaattg atctttttcc ctctgccaaa aattatgggg acatcatgaa gccccttgag
     5581 catctgactt ctggctaata aaggaaattt attttcattg caatagtgtg ttggaatttt
     5641 ttgtgtctct cactcggaag gacatatggg agggcaaatc atttaaaaca tcagaatgag
     5701 tatttggttt agagtttggc aacatatgcc catatgctgg ctgccatgaa caaaggttgg
     5761 ctataaagag gtcatcagta tatgaaacag ccccctgctg tccattcctt attccataga
     5821 aaagccttga cttgaggtta gatttttttt atattttgtt ttgtgttatt tttttcttta
     5881 acatccctaa aattttcctt acatgtttta ctagccagat ttttcctcct ctcctgacta
     5941 ctcccagtca tagctgtccc tcttctctta tggagatccc tcgacctgca gcccaagctt
     6001 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     6061 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     6121 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagcg
     6181 gatccgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     6241 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     6301 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     6361 ggaggcctag gcttttgcaa aaagctaaca taacttcgta tagcatacat tatacgaagt
     6421 tatgagctcg aattcagatc tcgagcggga tcaattccgc ccccccccta acgttactgg
     6481 ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt
     6541 gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc
     6601 taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc
     6661 agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg
     6721 gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc
     6781 tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa
     6841 atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg
     6901 tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa
     6961 aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgataata
     7021 ccatggaaga tgccaaaaac attaagaagg gcccagcgcc attctaccca ctcgaagacg
     7081 ggaccgccgg cgagcagctg cacaaagcca tgaagcgcta cgccctggtg cccggcacca
     7141 tcgcctttac cgacgcacat atcgaggtgg acattaccta cgccgagtac ttcgagatga
     7201 gcgttcggct ggcagaagct atgaagcgct atgggctgaa tacaaaccat cggatcgtgg
     7261 tgtgcagcga gaatagcttg cagttcttca tgcccgtgtt gggtgccctg ttcatcggtg
     7321 tggctgtggc cccagctaac gacatctaca acgagcgcga gctgctgaac agcatgggca
     7381 tcagccagcc caccgtcgta ttcgtgagca agaaagggct gcaaaagatc ctcaacgtgc
     7441 aaaagaagct accgatcata caaaagatca tcatcatgga tagcaagacc gactaccagg
     7501 gcttccaaag catgtacacc ttcgtgactt cccatttgcc acccggcttc aacgagtacg
     7561 acttcgtgcc cgagagcttc gaccgggaca aaaccatcgc cctgatcatg aacagtagtg
     7621 gcagtaccgg attgcccaag ggcgtagccc taccgcaccg caccgcttgt gtccgattca
     7681 gtcatgcccg cgaccccatc ttcggcaacc agatcatccc cgacaccgct atcctcagcg
     7741 tggtgccatt tcaccacggc ttcggcatgt tcaccacgct gggctacttg atctgcggct
     7801 ttcgggtcgt gctcatgtac cgcttcgagg aggagctatt cttgcgcagc ttgcaagact
     7861 ataagattca atctgccctg ctggtgccca cactatttag cttcttcgct aagagcactc
     7921 tcatcgacaa gtacgaccta agcaacttgc acgagatcgc cagcggcggg gcgccgctca
     7981 gcaaggaggt aggtgaggcc gtggccaaac gcttccacct accaggcatc cgccagggct
     8041 acggcctgac agaaacaacc agcgccattc tgatcacccc cgaaggggac gacaagcctg
     8101 gcgcagtagg caaggtggtg cccttcttcg aggctaaggt ggtggacttg gacaccggta
     8161 agacactggg tgtgaaccag cgcggcgagc tgtgcgtccg tggccccatg atcatgagcg
     8221 gctacgttaa caaccccgag gctacaaacg ctctcatcga caaggacggc tggctgcaca
     8281 gcggcgacat cgcctactgg gacgaggacg agcacttctt catcgtggac cggctgaaga
     8341 gcctgatcaa atacaagggc taccaggtag ccccagccga actggagagc atcctgctgc
     8401 aacaccccaa catcttcgac gccggggtcg ccggcctgcc cgacgacgat gccggcgagc
     8461 tgcccgccgc agtcgtcgtg ctggaacacg gtaaaaccat gaccgagaag gagatcgtgg
     8521 actatgtggc cagccaggtt acaaccgcca agaagctgcg cggtggtgtt gtgttcgtgg
     8581 acgaggtgcc taaaggactg accggcaagt tggacgcccg caagatccgc gagattctca
     8641 ttaaggccaa gaagggcggc aagatcgccg tgtagggcgg ccgcgactct agagtcgagt
     8701 ctagatatca gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga
     8761 gcatctgact tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt
     8821 tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttaaaac atcagaatga
     8881 gtatttggtt tagagtttgg caacatatgc ccatatgctg gctgccatga acaaaggttg
     8941 gctataaaga ggtcatcagt atatgaaaca gccccctgct gtccattcct tattccatag
     9001 aaaagccttg acttgaggtt agattttttt tatattttgt tttgtgttat ttttttcttt
     9061 aacatcccta aaattttcct tacatgtttt actagccaga tttttcctcc tctcctgact
     9121 actcccagtc atagctgtcc ctcttctctt atggagatcc ctcgacctgc agcccaagct
     9181 tctgcgaaga tacggccacg ggtgctcttg atcctgtggc tgattttgga ctgtgctgct
     9241 cgcagctgct gatgaatcac atacttcctc cattttcttc cactgattga ctgttataat
     9301 ttccctaatt tccaggtcaa ggtgctgtgc attgtggtaa tagatgtgac atgacgtcac
     9361 ttccaaagga ccaatgaaca tgtctgacca atttcatata atgtgaaaac gattttcata
     9421 ggcagaataa ataacattta aattaaactg ggcatcagcg caattcaatt ggtttggtaa
     9481 tagcaaggga aaatagaatg aagtgatctc caaaaaataa gtactttttg actgtaaata
     9541 aaattgtaag gagtaaaaag tacttttttt tctaaaaaaa tgtaattaag taaaagtaaa
     9601 agtattgatt tttaattgta ctcaagtaaa gtaaaaatcc ccaaaaataa tacttaagta
     9661 cagtaatcaa gtaaaattac tcaagtactt tacacctctg gttcttgacc ccctaccttc
     9721 agcaagccca gcagatccac tagttctaga gcggccgcca ccgcggtgga gctccagctt
     9781 ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc
     9841 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     9901 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     9961 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
    10021 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
    10081 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
    10141 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
    10201 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
    10261 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
    10321 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
    10381 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
    10441 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
    10501 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
    10561 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
    10621 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
    10681 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
    10741 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
    10801 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
    10861 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
    10921 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
    10981 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
    11041 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
    11101 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
    11161 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
    11221 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
    11281 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
    11341 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
    11401 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
    11461 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
    11521 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
    11581 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
    11641 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
    11701 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
    11761 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
    11821 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
    11881 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
    11941 aggggttccg cgcacatttc cccgaaaagt g
//
