LOCUS       sequence-210-exp        8775 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8775)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1997
REFERENCE   2  (bases 1 to 8775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: L3811
FEATURES             Location/Qualifiers
     source          1..8775
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1405..1433)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2181..2201
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     promoter        6520..6624
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6625..6693,6694..7485)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6843..6862)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7656..8244
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8145..8164
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8398..8415
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8532..8553
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8568..8573,8574..8591,8592..8598)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8606..8622
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8611..8633
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8630..8646
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8630..8646
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcctgcaggt cgaggcattt gaattggggg tggtggacag taactgtctg
       61 taataataat tactcctgac caggttgcaa ttcgagtttt gataagcata attatacctt
      121 gtacattgtg ggttttgtgc tgtggacgtt ttattgtgac atccccataa gctacaagaa
      181 caaaaatgaa atgtaaaagt attgaaaacg tcgtacattt tatatctgag tagtatcctt
      241 tgctttaaat gtccataaaa ctaattttat aatcaataaa acaacgtttg taaatcaact
      301 gagtttacaa gtagagacat tgagggatac tttcactatg ctaaagtgat aatcgaccaa
      361 ataataactt cactttggta tttattcctg tcttataaat gttatgtatg aattaaattc
      421 atatgcatat ggctcactct gacaattttt aataatcttc cagatcaata ttgactaccg
      481 atgcgggtgg tcttttgctt tgaattctgc tgaactttac accccgaaca gcaatgtgtg
      541 cttcagccta aaaaaaagta agtgtgttaa tcagtgcccc cgattcttca ttttttgccc
      601 ctctctcccg tttcgtcggc aaaagaagag aaaataaaga taagtctcaa gataggttgg
      661 taatcgctaa agtggttgtg tggataagag tagcaaaatg gcaggaagag cactttgcgc
      721 gcacacactg tactcattgt tctggataaa attctctcgt tgtttgccgt cggatgtctg
      781 cctctctgca ttgagccggc ttcttcacta tctttagtta acctaaaatg ccgtttcttt
      841 tctcgtatcc ccactatccc gttgaggttc tctgctctct tcgctcccta ccgccagcga
      901 gcaactatcc gtgggggcgc cttgctcgga agatgggggg gaagaaagaa gatttttgct
      961 atttgcactt gagaaagaga cttttcctgc gtcgatggtt agagaacagt gtgcagacac
     1021 ttttcagcta cctagaatta caattggata tccccgcctc ccaatccacc cacccaggga
     1081 aaaagaaggg ctcgccgaaa atcaaagtta tctccaggct cgcgcatccc accgagcggt
     1141 tgacttctct ccaccacttt tcattttaac cctcggggta cgggattggc caaaggaccc
     1201 aaaggtatgt ttcgaatgat actaacataa catagaacat tttcaggagg acccttggct
     1261 agcgtcgacg gtaccggtca gatatcactc gaggagctcc cgagatccta tcgattcgcg
     1321 gccgctgtac acccgggtgc cctatagtga gtcgtattgg taccggtaga aaaaatgagt
     1381 aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt
     1441 aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacata cggaaaactt
     1501 acccttaaat ttatttgcac tactggaaaa ctacctgttc catgggtaag tttaaacata
     1561 tatatactaa ctaaccctga ttatttaaat tttcagccaa cacttgtcac tactttctgt
     1621 tatggtgttc aatgcttctc gagataccca gatcatatga aacagcatga ctttttcaag
     1681 agtgccatgc ccgaaggtta tgtacaggaa agaactatat ttttcaaaga tgacgggaac
     1741 tacaagacac gtaagtttaa acagttcggt actaactaac catacatatt taaattttca
     1801 ggtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt aaaaggtatt
     1861 gattttaaag aagatggaaa cattcttgga cacaaattgg aatacaacta taactcacac
     1921 aatgtataca tcatggcaga caaacaaaag aatggaatca aagttgtaag tttaaacatg
     1981 attttactaa ctaactaatc tgatttaaat tttcagaact tcaaaattag acacaacatt
     2041 gaagatggaa gcgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc
     2101 cctgtccttt taccagacaa ccattacctg tccacacaat ctgccctttc gaaagatccc
     2161 aacgaaaaga gagaccacat ggtccttctt gagtttgtaa cagctgctgg gattacacat
     2221 ggcatggacg aactatacaa aagcccacaa gctttggaat tcgctagccg gccatacaag
     2281 taatccgggt tctagaagcg ctgctaaggg ggccctcgtc gagtcggtca caatcacctg
     2341 aaactccaaa ggcagccagt gaggaacgtg aagaagaaga aaaagagtca tctgaacagg
     2401 tttgattttc tttctggtca aaaagatgaa attattgatt ttcagccaga tactcccaaa
     2461 actagcagcg agaagtctgc aagtcgttca cagtcgccca gagaatcgcg ggaagtgagc
     2521 caagaggtat gtttttcaaa aatcaataac tgatcataat ttttattgtt tggtgaattt
     2581 aagaaaataa tattcgaaaa ttcctctgaa ttatcaagat tgcagtatta atttcgagaa
     2641 aaattgagat attcatagag ctattgtaaa ttttcttgat ttcagactga aacttcggaa
     2701 aatcaagaga aaatcaaaga aaaggatgac ggggatgatc agcctggcac accgaacagc
     2761 tatagaagcc gggaaacttc accagctcca aaaaggtcca aggagaccag gtttgtcaaa
     2821 agcttcctgc gattaattct catttcaatt tttcagagaa tcagagtctc ctgaaaaatc
     2881 cccggttcgt tcaagatctc ccagaaggtc ttcagcacgt tccccgtcac gatctcctag
     2941 acggcgccga gaaagaagct cagaaagaaa gcaatccgaa gagccagcac cgctaccaga
     3001 gaaaaagaag aaagagccgc tggatattct acgaacaaga accggaggag catatattcc
     3061 acccgccaaa cttcgactta tgcaacaaca gattagtgat aagcaaagtg aacagtatca
     3121 gagaatgaat tgggaaagaa tgaagaaaaa gattcacgga ttggttaaca gagtcaacgc
     3181 gaagaatctt gttcaaattg tcagagaact tcttcaagag aatgtgattc gttcaaagtg
     3241 agtgagaaaa tcgaaggaaa aggaaagaat taatttaatt tttcagggga cttctctgcc
     3301 gtgacattat tcaagctcag gctttctcac caggattctc taacgtctat gcagctttgg
     3361 cggcagttat caactcgaaa ttccctcatg tcggtgaact tcttctccgt cgtctgattg
     3421 tacagttcaa aagaagtttc cgtagaaatg acagaggcgt cacggtgaac gtgatcaaat
     3481 tcatcgcaca tttgattaat caacaagttg ctcacgaagt tcttgcgctg gaaatcatga
     3541 ttctgatgct tgaagaacca actgatgatt cagttgaagt cgccattgcg ttcctgaaag
     3601 agtgtggagc aaagcttctg gagattgctc cagcagctct taacagtgtc tacgaccgtc
     3661 ttcgtgcaat tctcatggaa actgaaagat cggaaaatgc actggatcga cgtattcagt
     3721 atatgattga gactgcaatg cagattcgaa aggacaaatt tgcggcgtat ccggcggtca
     3781 ttgaagactt ggacttgatt gaggaggagg atcagatcat ccatacactt aatttggagg
     3841 atgcggttga tccggaaaat gggcttaatg tgttcaaact agatccagaa ttcgaaaaga
     3901 acgaggaggt ttatgaggag atccgtaagg aaatcattgg aaacgccgat atttcggatg
     3961 aggatggtgg cgacgagttg gatgatgaag aagagggtag tgatgtggaa gaggctccga
     4021 agaagactac agagattatt gataatactg atcagaattg actgctttca gaaggtattc
     4081 attttgagtt ttgggccggc aaatctgtaa gttgccggtt gccgaaaatt tgctgaattt
     4141 gccggaaaaa aaaattccgg aatttattta aaaacttttt gtaaaaatta aattaaattt
     4201 gcaacttttc agagaagtct acctgacaat gcaatcatct ttggactacc aagaagctgc
     4261 tcacaaattg ctgaaaatga agattccaga cagcatgcag gtcagcgatg ttgcaaagaa
     4321 aaattttcga ccaaaaaaac caaccaatca taaaatttaa aaaaaaactc cgtttttttc
     4381 ttttttttta tacgagaaaa accaaaaaaa tgtatttttg ccaaattcta aaatactatc
     4441 cccgaaattt tcaatatttt ctctttcaga acgaactctg cgcgatgctt gtcgattgtt
     4501 gtgctcaaca gcgtacctac gagcgattct acggaatgct catcgaacgt ttctgccgac
     4561 ttcgcctcga ataccagcaa tactttgaaa agctctgcca ggacacgtat tccacgattc
     4621 accgaattga catcacaaaa ctgcggaatt tggctcgcct tattgctcat ttgctctcga
     4681 cggatgctat tgactggaag attttggccg atatgaaaat gaccgaagag gacacaactt
     4741 cttctggcag aatctatatt aaatatatat ttaatgaact tgtggaggcg atgggaatgg
     4801 ttaaacttca ttcgagagtt actgatccgt gagtttccta gagagagttg ttttcgtatt
     4861 caattttccc tattttcaga actttggctc attgctttgt tggattattc ccacgaacta
     4921 atccgaacag cgcacgattt tcgatcaact tcttcacaat gattggattg ggtggtttga
     4981 cgttggaact tcgtgaatgg ctggcaaagg gtctcaagaa gaagaaggga atgctggatc
     5041 agttgaaggc cgaatcaagc tcagattcat cgtcgtcttc ggattcgtca gactcgtctg
     5101 attcttcgga ttctgacgat tcatccgact cgtcttcaga ttcctcatct tcttcagaat
     5161 cagagccaga accaccgaag aaaaagaaga agaagaacag tgaagagagt tccaaaaaga
     5221 aggaaaaaga gaatattggt cgacgggatc gtggagacaa gagagctgaa cgtcatcgtg
     5281 atcaaagtgt ggagaacaag gacaaggatc gtcgacgtcg ccaggattct gacgaaaatc
     5341 gtcggccaga acgaggagat gaccgcaagg atcggagtaa agatcgtcgt cgtcaagact
     5401 cggatgatga ggatcggaaa ggtcgtgaac gtcgggaaga ttcaggggaa agacgtcgcg
     5461 gagatcggga tcgacgtgat cgaaacaagg atcaggagga tcaccgtgaa gatcgccgtg
     5521 accgaagcaa ggatcgtgag gatcgacgtg atcgccgtcg tcatgactct gatgatgatc
     5581 gtaaaactcg tcgggataga agtgaagagc gaggaggacg tcgtcgtgaa gtggaatcgg
     5641 atgatcgacg ccgacgtcgt tgaattttca aattttaaat actgaatatt tgtttttttt
     5701 cctattattt atttattctc tttgtgtttt ttttcttgct ttctaaaaaa ttaattcaat
     5761 ccaaatctaa acattttttt ttctctttcc gtctcccaat tcgtattccg ctcctctcat
     5821 ctgaacacaa tgtgcaagtt tatttatctt ctcgctttca tttcattagg acgtgggggg
     5881 aattggtgga agggggaaac acacaaaagg atgatggaaa tgaaataagg acacacaata
     5941 tgcaacaaca ttcaattcag aaatatggag gaaggtttaa aagaaaacat aaaaatatat
     6001 agaggaggaa ggaaaactag taaaaaataa gcaaagaaat taggcgaacg atgagaattg
     6061 tcctcgcttg gcaaatgcga atccgtatgg agaggcacgt ttggcgaagg caaatgttcg
     6121 gtatggagat ctgtaaaaat ttttaagttg aaatttggtg ttgctctttt acaaaatttt
     6181 ccgattttcg cttgaaatta cggtgccagg tctcgacacg tcttccaatt tttcaaattc
     6241 aaaagagcct ttaatgggct gtagttgcta atttctcgtt tttgaaaatt tttcttccgt
     6301 ttaatcgaaa tttgatgtat tttatttatg attttcaata aatttcaaag aaactggtga
     6361 aaactcggaa aattgtgaac tacagtaatc caatccttaa aggcgcacac cttttaaatg
     6421 tccgccccaa tacgatattt ttttaagatt cgctagagcg gcccaaaagg cctttttttt
     6481 ttggcgccga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     6541 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     6601 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     6661 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     6721 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     6781 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     6841 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     6901 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     6961 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     7021 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     7081 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     7141 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     7201 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     7261 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     7321 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     7381 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     7441 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     7501 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     7561 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     7621 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     7681 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     7741 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     7801 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     7861 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     7921 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     7981 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     8041 cgtgagcatt gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     8101 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     8161 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     8221 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     8281 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     8341 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     8401 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     8461 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     8521 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     8581 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
     8641 tatgaccatg attacgccaa gctgtaagtt taaacatgat cttactaact aactattctc
     8701 atttaaattt tcagagctta aaaatggctg aaatcactca caacgatgga tacgctaaca
     8761 acttggaaat gaaat
//
