LOCUS       sequence-210055-        9310 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression plasmid of sgRNA with mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9310)
  AUTHORS   Tarumoto Y, Lu B, Somerville TDD, Huang YH, Milazzo JP, Wu XS, 
            Klingbeil O, El Demerdash O, Shi J, Vakoc CR
  TITLE     LKB1, Salt-Inducible Kinases, and MEF2C Are Linked Dependencies in 
            Acute Myeloid Leukemia.
  JOURNAL   Mol Cell. 2018 Feb 28. pii: S1097-2765(18)30109-6. doi: 
            10.1016/j.molcel.2018.02.011.
  PUBMED    29526696
REFERENCE   2  (bases 1 to 9310)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2018 Feb 28. pii: S1097-2765(18)30109-6. doi: 
            10.1016/j.molcel.2018.02.011."
COMMENT     Sequence Label: LRCherry2.1
FEATURES             Location/Qualifiers
     source          1..9310
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        12..238
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             239..419
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    466..591
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1084..1317
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1502..1546
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1724..1972
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1724..1744
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     1895..1914
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    3998..4115
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4184..4395
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      4415..4424
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4424..4447
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4448..5158
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4593..4611)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4861..4880
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    5174..5762
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5227..5247)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5645..5656)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5834..6067
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6145..6266
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6182..6201)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6236..6255
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6306..6441
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6461..6480)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6462..6480)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6490..6507)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6490..6506)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6499..6521)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6648..7103
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6735..6754)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6945..6966
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7129..7233
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7234..7302,7303..8094)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7452..7471)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8265..8853
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8754..8773
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9007..9024
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9141..9162
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9177..9182,9183..9200,9201..9207)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9215..9231
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9220..9242
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9239..9255
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9239..9255
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9274..9294
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9276..9294
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgcaagctta atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag
       61 ttagcaacat gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag
      121 gtggtacgat cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac
      181 cactgaattg ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg
      241 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
      301 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
      361 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
      421 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
      481 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
      541 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
      601 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
      661 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
      721 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
      781 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
      841 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
      901 aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga
      961 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     1021 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     1081 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     1141 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     1201 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     1261 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     1321 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     1381 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     1441 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     1501 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     1561 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     1621 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     1681 attatcgttt cagacccacc tcccaacccc gaggggaccc agagagggcc tatttcccat
     1741 gattccttca tatttgcata tacgatacaa ggctgttaga gagataatta gaattaattt
     1801 gactgtaaac acaaagatat tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg
     1861 gtagtttgca gttttaaaat tatgttttaa aatggactat catatgctta ccgtaacttg
     1921 aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgaaaca ccggagacgg
     1981 ttgtaaatga gcacacaaaa tacacatgct aaaatattat attctatgac ctttataaaa
     2041 tcaaccaaaa tcttcttttt aataacttta gtatcaataa ttagaatttt tatgttcctt
     2101 tttgcaaact tttaataaaa atgagcaaaa taaaaaaacg ctagttttag taactcgcgt
     2161 tgttttcttc acctttaata atagctactc caccacttgt tcctaagcgg tcagctcctg
     2221 cttcaatcat tttttgagca tcttcaaatg ttctaactcc accagctgct ttaactaaag
     2281 cattgtcttt aacaactgac ttcattagtt taacatcttc aaatgttgca cctgattttg
     2341 aaaatcctgt tgatgtttta acaaattcta atccagcttc aacagctatt tcacaagctt
     2401 tcatgatttc ttcttttgtt aataaacaat tttccataat acatttaaca acatgtgatc
     2461 cagctgcttt ttttacagct ttcatgtctt ctaaaactaa ttcataattt ttgtctttta
     2521 atgcaccaat atttaatacc atatcaattt ctgttgcacc atctttaatt gcttcagaaa
     2581 cttcgaatgc ttttgtagct gttgtgcatg cacctagagg aaaacctaca acatttgtta
     2641 ttcctacatt tgtgcctttt aataattctt tacaatagct tgttcaatat gaattaacac
     2701 aaactgttgc aaaatcaaat tcaattgctt catcacataa ttgtttaatt tcagctttcg
     2761 tagcatcttg ttttaataat gtgtgatcta tatatttgtt tagtttcatt ttttctccta
     2821 tatattcatt tttaatttta attctttaat aatttcgtct actttaactt tagcgttttg
     2881 aacagattca ccaacaccta taaaataaat ttttagttta ggttcagttc cacttgggcg
     2941 aacagcaaat catgacttat cttctaaata aaattttagt aagtcttgtc ctggcatatt
     3001 atacattcca tcgatgtagt cttcaacatt aacaacttta agtccagcaa tttgagttaa
     3061 gggtgttgct ctcaatgatt tcattaatgg ttcaattttt aatttctttt cttctggttt
     3121 aaaattcaag tttaaagtga aagtgtaata tgcacccatt tctttaaata aatcttctaa
     3181 atagtctact aatgttttat tttgtttttt ataaaatcaa gcagcctctg ctattaatat
     3241 agaagcttgt attccatctt tatctctagc tgagtcatca attacatatc cataactttc
     3301 ttcataagca aaaacaaaat ttaatccgtt atcttcttct ttagcaattt ctctacccat
     3361 tcatttaaat ccagttaaag tttttacaat attaactcca tatttttcat gagcgattct
     3421 atcacccaaa tcacttgtta caaaacttga atatagagcc ggattttttg gaatgctatt
     3481 taagcgtttt agatttgata attttcaatc aattaaaatt ggtcctgttt gatttccatc
     3541 taatcttaca aaatgaccat catgttttat tgccattcca aatctgtcag catctgggtc
     3601 attcataata ataatatctg catcatgttt aataccatat tcaagcggta tttttcatgc
     3661 aggatcaaat tctggatttg gatttacaac atttttaaat gtttcatctt caaatgcatg
     3721 ctcttcaacc tcaataacgt tatatcctga ttcacgtaat atttttgggg taaatttagt
     3781 tcctgttcca ttaactgcgc taaaaataat ttttaaatct tttttagctt cttgctcttt
     3841 tttgtacgtc tctgtttaag agctatgctg gaaacagcat agcaagttta aataaggcta
     3901 gtccgttatc aactttgctg gaaacagcaa agtggcaccg agtcggtgct ttttttgaat
     3961 tctagatctt gagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
     4021 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
     4081 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
     4141 atccactttg gcgccggctc gagtggctcc ggtgcccgtc agtgggcaga gcgcacatcg
     4201 cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg
     4261 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt
     4321 gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt
     4381 gccgccagaa cacaggtgtc gtgacgcggg atccgccacc atggattaca aagacgatga
     4441 cgataagatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg
     4501 cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg
     4561 cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc
     4621 cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt
     4681 gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg
     4741 ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct
     4801 gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg
     4861 ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga
     4921 ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta
     4981 cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta
     5041 caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca
     5101 gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt acaagtagac
     5161 gcgttaagtc gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     5221 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     5281 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     5341 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     5401 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     5461 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     5521 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt
     5581 tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     5641 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     5701 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     5761 gcgtcgactt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     5821 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct
     5881 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     5941 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     6001 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     6061 tctagcagta cgtatagtag ttcatgtcat cttattattc agtatttata acttgcaaag
     6121 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa
     6181 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     6241 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc
     6301 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     6361 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     6421 gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta
     6481 cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     6541 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     6601 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag
     6661 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     6721 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     6781 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     6841 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     6901 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     6961 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     7021 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     7081 caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct
     7141 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     7201 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     7261 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     7321 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     7381 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     7441 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     7501 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     7561 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     7621 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     7681 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     7741 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     7801 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     7861 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     7921 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     7981 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     8041 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     8101 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     8161 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     8221 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     8281 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     8341 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     8401 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     8461 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     8521 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     8581 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     8641 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     8701 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     8761 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     8821 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     8881 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     8941 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     9001 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     9061 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     9121 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     9181 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     9241 ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa
     9301 aagctggagc
//
