LOCUS       sequence-210062-        3538 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Preparation of RNA probe for ISH of mouse Vmn2r76 gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3538)
  AUTHORS   Akiyoshi S, Ishii T, Bai Z, Mombaerts P
  TITLE     Subpopulations of vomeronasal sensory neurons with coordinated 
            coexpression of type 2 vomeronasal receptor genes are differentially
            dependent on Vmn2r1.
  JOURNAL   Eur J Neurosci. 2018 Apr;47(7):887-900. doi: 10.1111/ejn.13875. Epub
            2018 Mar 25.
  PUBMED    29465786
REFERENCE   2  (bases 1 to 3538)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/ejn.13875";
            journalName: "Eur J Neurosci"; date: "2018-04"; volume: "47"; issue:
            "7"; pages: "887-900"
COMMENT     Sequence Label: pISH-Vmn2r76
FEATURES             Location/Qualifiers
     source          1..3538
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(663..681)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(664..681)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(699..715)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(699..715)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(712..734)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    723..739
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(747..753,754..771,772..777))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    792..813
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(930..947)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1101..1689)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1181..1200)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1860..2651,2652..2720))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2483..2502
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2721..2825)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2903..3358)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3040..3061)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3252..3271
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3484..3506
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3498..3515
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3499..3515
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(3522..3538,1..3)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        join(3522..3538,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggcgaattg ggcccgacgt cgcatgctcc cggccgccat ggcggccgcg ggaattcgat
       61 tccatttcac cctcttctca gtagctttga acactttcct tatctgtacc agatagctat
      121 caaggacaca tctgtagcta ttggcatggt atctttggtg atttatttcg gatggaactg
      181 ggtgggactg atgatttcag atgacggtct aggacttcaa tttgcctctg aattgagaga
      241 agaaatgcaa agatatgaca tctgtttagc ctttgtgact attatcacat ataacacaaa
      301 attattcttg acaatgactg acacgtatta caatcagatc atgatgtctt tagcaaaagc
      361 tgttatcatt tttggagaca aagattctct tctacaagtg atttttagac tgtggcagtt
      421 tttagacatt cgaagaatct gggttactac ctcacagtgg gatatcatta caagtaatgg
      481 agaattcctc ttcaactcct ttcatggtac cctcagcttt tcacaccatt attctgagat
      541 agctggtttt aaagaattta tacagacagc gcacccttta aaaatcacta gtgaattcgc
      601 ggccgcctgc aggtcgacca tatgggagag ctcccaacgc gttggatgca tagcttgagt
      661 attctatagt gtcacctaaa tagcttggcg taatcatggt catagctgtt tcctgtgtga
      721 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
      781 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
      841 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
      901 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
      961 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     1021 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     1081 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     1141 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     1201 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     1261 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     1321 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     1381 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     1441 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     1501 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     1561 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     1621 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     1681 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     1741 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     1801 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     1861 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     1921 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     1981 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     2041 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     2101 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     2161 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     2221 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     2281 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     2341 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     2401 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     2461 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     2521 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     2581 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     2641 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     2701 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     2761 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     2821 ccgcgcacat ttccccgaaa agtgccacct gatgcggtgt gaaataccgc acagatgcgt
     2881 aaggagaaaa taccgcatca ggaaattgta agcgttaata ttttgttaaa attcgcgtta
     2941 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
     3001 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
     3061 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
     3121 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta
     3181 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
     3241 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
     3301 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc
     3361 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     3421 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     3481 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gtaatacgac tcactata
//
