LOCUS       sequence-21050-e         641 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 641)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG423GAL-ccdB-Cerulean
FEATURES             Location/Qualifiers
     source          1..641
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(41..165)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(173..189)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(173..189)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(210..233)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
ORIGIN
        1 agcataaagt gtaaagcctg ggggtgccta atgcggccgc catagtgact ggatatgttg
       61 tgttttacag tattatgtag tctgtttttt atgcaaaatc taatttaata tattgatatt
      121 tatatcattt tacgtttctc gttcagcttt tttgtacaaa cttgtgatgg gggatccact
      181 agttctagaa tccggggttt tttctccttg acgttaaagt atagaggtat attaacaatt
      241 ttttgttgat acttttatta catttgaata agaagtaata caaaccgaaa atgttgaaag
      301 tattagttaa agtggttatg cagtttttgc atttatatat ctgttaatag atcaaaaatc
      361 atcgcttcgc tgattaatta ccccagaaat aaggctaaaa aactaatcgc attatcatcc
      421 tatggttgtt aatttgattc gttcatttga aggtttgtgg ggccaggtta ctgccaattt
      481 ttcctcttca taaccataaa agctagtatt gtagaatctt tattgttcgg agcagtgcgg
      541 cgcgaggcac atctgcgttt caggaacgcg accggtgaag acgaggacgc acggaggaga
      601 gtcttccttc ggagggctgt cccccgctcg gcggcttcta a
//
