LOCUS       sequence-210656-        7421 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Alpha Actinin, Excitation: 400 / 490, Emission: 468 / 
            511.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7421)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 7421)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PS-CFP2-Alpha-Actinin-19
FEATURES             Location/Qualifiers
     source          1..7421
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        54..357
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        358..561
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     512..532
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             3363..4079
                     /codon_start=1
                     /product="photoswitchable cyan-to-green fluorescent 
                     protein"
                     /label=PS-CFP2
                     /note="mammalian codon-optimized"
                     /translation="MSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVATLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDDG
                     NYKTRAEVKFEGDTLVSRIELTGTDFKEDGNILGNKMEYNYNATNVYIVADKARNGIKV
                     NFKVRHNIKDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYLE
                     FVTAAAITHGMDELYK"
     polyA_signal    4202..4323
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4239..4258)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4293..4312
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4330..4785)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4467..4488)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4679..4698
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4812..4916
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4918..5275
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4918..5109
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4941..4961)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5126..5261
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5310..6104
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5310..6104
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5364..5383)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5974..5993
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6292..6311)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6336..6383
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6712..7300
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7201..7220
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
       61 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
      121 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
      181 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
      241 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
      301 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
      361 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
      421 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
      481 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
      541 gaggtctata taagcagagc tggtttagtg aaccgtcaga tccgctagcg ctaccggact
      601 cagatctcga gctcaagctt cgcaccatca tggaccatta tgattctcag caaaccaacg
      661 attacatgca gccagaagag gactgggacc gggacctgct cctggacccg gcctgggaga
      721 agcagcagag aaagacattc acggcatggt gtaactccca cctccggaag gcggggacac
      781 agatcgagaa catcgaagag gacttccggg atggcctgaa gctcatgctg ctgctggagg
      841 tcatctcagg tgaacgcttg gccaagccag agcgaggcaa gatgagagtg cacaagatct
      901 ccaacgtcaa caaggccctg gatttcatag ccagcaaagg cgtcaaactg gtgtccatcg
      961 gagccgaaga aatcgtggat gggaatgtga agatgaccct gggcatgatc tggaccatca
     1021 tcctgcgctt tgccatccag gacatctccg tggaagagac ttcagccaag gaagggctgc
     1081 tcctgtggtg tcagagaaag acagcccctt acaaaaatgt caacatccag aacttccaca
     1141 taagctggaa ggatggcctc ggcttctgtg ctttgatcca ccgacaccgg cccgagctga
     1201 ttgactacgg gaagctgcgg aaggatgatc cactcacaaa tctgaatacg gcttttgacg
     1261 tggcagagaa gtacctggac atccccaaga tgctggatgc cgaagacatc gttggaactg
     1321 cccgaccgga tgagaaagcc atcatgactt acgtgtctag cttctaccac gccttctctg
     1381 gagcccagaa ggcggagaca gcagccaatc gcatctgcaa ggtgttggcc gtcaaccagg
     1441 agaacgagca gcttatggaa gactacgaga agctggccag tgatctgttg gagtggatcc
     1501 gccgcacaat cccgtggctg gagaaccggg tgcccgagaa caccatgcat gccatgcaac
     1561 agaagctgga ggacttccgg gactaccggc gcctgcacaa gccgcccaag gtgcaggaga
     1621 agtgccagct ggagatcaac ttcaacacgc tgcagaccaa gctgcggctc agcaaccggc
     1681 ctgccttcat gccctctgag ggcaggatgg tctcggacat caacaatgcc tggggctgcc
     1741 tggagcaggt ggagaagggc tatgaggagt ggttgctgaa tgagatccgg aggctggagc
     1801 gactggacca cctggcagag aagttccggc agaaggcctc catccacgag gcctggactg
     1861 acggcaaaga ggccatgctg cgacagaagg actatgagac cgccaccctc tcggagatca
     1921 aggccttgct caagaagcat gaggccttcg agagtgacct ggctgcccac caggaccgtg
     1981 tggagcagat tgccgccatc gcacaggagc tcaatgagct ggactattat gactcaccca
     2041 gtgtcaacgc ccgttgccaa aagatctgtg accagtggga caatctgggg gccctaactc
     2101 agaagcgaag ggaagctctg gagcggaccg agaaactgct ggagaccatt gaccagctgt
     2161 acttggagta tgccaagcgg gctgcaccct tcaacaactg gatggagggg gccatggagg
     2221 acctgcagga caccttcatt gtgcacacca ttgaggagat ccagggactg accacagccc
     2281 atgagcagtt caaggccacc ctccctgatg ccgacaagga gcgcctggcc atcctgggca
     2341 tccacaatga ggtgtccaag attgtccaga cctaccacgt caatatggcg ggcaccaacc
     2401 cctacacaac catcacgcct caggagatca atggcaaatg ggaccacgtg cggcagctgg
     2461 tgcctcggag ggaccaagct ctgacggagg agcatgcccg acagcagcac aatgagaggc
     2521 tacgcaagca gtttggagcc caggccaatg tcatcgggcc ctggatccag accaagatgg
     2581 aggagatcgg gaggatctcc attgagatgc atgggaccct ggaggaccag ctcagccacc
     2641 tgcggcagta tgagaagagc atcgtcaact acaagccaaa gattgatcag ctggagggcg
     2701 accaccagct catccaggag gcgctcatct tcgacaacaa gcacaccaac tacaccatgg
     2761 agcacatccg tgtgggctgg gagcagctgc tcaccaccat cgccaggacc atcaatgagg
     2821 tagagaacca gatcctgacc cgggatgcca agggcatcag ccaggagcag atgaatgagt
     2881 tccgggcctc cttcaaccac tttgaccggg atcactccgg cacactgggt cccgaggagt
     2941 tcaaagcctg cctcatcagc ttgggttatg atattggcaa cgacccccag ggagaagcag
     3001 aatttgcccg catcatgagc attgtggacc ccaaccgcct gggggtagtg acattccagg
     3061 ccttcattga cttcatgtcc cgcgagacag ccgacacaga tacagcagac caagtcatgg
     3121 cttccttcaa gatcctggct ggggacaaga actacattac catggacgag ctgcgccgcg
     3181 agctgccacc cgaccaggct gagtactgca tcgcgcggat ggccccctac accggccccg
     3241 actccgtgcc aggtgctctg gactacatgt ccttctccac ggcgctgtac ggcgagagtg
     3301 acctcaagct tcgaattctg cagtcgacgg taccgcgggc ccgggatcca ccggtcgcca
     3361 ccatgagcaa gggcgccgag ctgttcaccg gcatcgtgcc catcctgatc gagctgaatg
     3421 gcgatgtgaa tggccacaag ttcagcgtga gcggcgaggg cgagggcgat gccacctacg
     3481 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcctgtgccc tggcccaccc
     3541 tggtggccac cctgagctac ggcgtgcagt gcttctcacg ctaccccgat cacatgaagc
     3601 agcacgactt cttcaagagc gccatgcctg agggctacat ccaggagcgc accatcttct
     3661 tcgaggatga cggcaactac aagacgcgcg ccgaggtgaa gttcgagggc gataccctgg
     3721 tgagtcgcat cgagctgacc ggcactgatt tcaaggagga tggcaacatc ctgggcaata
     3781 agatggagta caactacaac gccaccaatg tgtacatcgt ggccgacaag gccaggaatg
     3841 gcatcaaggt gaacttcaag gtccgccaca acatcaagga tggcagcgtg cagctggccg
     3901 accactacca gcagaatacc cccatcggcg atggccctgt gctgctgccc gataaccact
     3961 acctgtccac ccagagcgcc ctgtccaagg accccaacga gaagcgcgat cacatgatct
     4021 acctcgagtt cgtgaccgcc gccgccatca cccacggcat ggatgagctg tacaagtaaa
     4081 gcggccgcga ctctagatca taatcagcca taccacattt gtagaggttt tacttgcttt
     4141 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
     4201 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     4261 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     4321 ttaaggcgta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa
     4381 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat
     4441 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg
     4501 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac
     4561 catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta
     4621 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag
     4681 ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg
     4741 taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc
     4801 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     4861 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtcctg
     4921 aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     4981 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     5041 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     5101 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     5161 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     5221 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaagatcg
     5281 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt
     5341 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     5401 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     5461 ccgacctgtc cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg
     5521 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     5581 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
     5641 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
     5701 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
     5761 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
     5821 tcgccaggct caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
     5881 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
     5941 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
     6001 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
     6061 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt
     6121 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc
     6181 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
     6241 gcgcggggat ctcatgctgg agttcttcgc ccaccctagg gggaggctaa ctgaaacacg
     6301 gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac
     6361 gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt
     6421 cgatacccca ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc
     6481 ccacccccca agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc
     6541 tgccatagcc tcaggttact catatatact ttagattgat ttaaaacttc atttttaatt
     6601 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     6661 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     6721 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     6781 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     6841 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     6901 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     6961 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     7021 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     7081 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     7141 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     7201 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     7261 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     7321 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     7381 tgattctgtg gataaccgta ttaccgccat gcattagtta t
//
