LOCUS       sequence-210690-        8815 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral  expression of shRNA targeting PBRM.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8815)
  AUTHORS   Gao W, Li W, Xiao T, Liu XS, Kaelin WG Jr
  TITLE     Inactivation of the PBRM1 tumor suppressor gene amplifies the 
            HIF-response in VHL-/- clear cell renal carcinoma.
  JOURNAL   Proc Natl Acad Sci U S A. 2017 Jan 31;114(5):1027-1032. doi: 
            10.1073/pnas.1619726114. Epub 2017 Jan 12.
  PUBMED    28082722
REFERENCE   2  (bases 1 to 8815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1619726114"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2017-01-31- 31"; volume: "114"; issue: "5"; pages: 
            "1027-1032"
COMMENT     Sequence Label: shPBRM1#131-Tet-pLKO-puro
FEATURES             Location/Qualifiers
     source          1..8815
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        82..176
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     primer_bind     93..112
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    130..148
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    158..176
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    276..393
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        442..952
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     871..889
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     intron          961..1533
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     1541..1560
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        1588..1606
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1617..2237
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(1690..1709)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     misc_feature    2272..2845
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2439..2456)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2666..2685
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2865..3464
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2865..2884)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3361..3381
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             3592..3825
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3897..4018
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3934..3953)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3988..4007
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4058..4193
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(4214..4232)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4242..4259)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4242..4258)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4251..4273)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4400..4855
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4487..4506)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4697..4718
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4881..4985
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4986..5054,5055..5846)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5204..5223)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6017..6605
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6506..6525
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6759..6776
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6893..6914
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6929..6934,6935..6952,6953..6959)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6967..6983
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6972..6994
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6991..7007
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6991..7007
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7026..7046
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7028..7046
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7074..7300
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7301..7481
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7528..7653
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8146..8379
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8564..8608
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgatcacga
       61 gactagcctc gagcggccgc aatatttgca tgtcgctatg tgttctggga aatcaccata
      121 aacgtgaaat ccctatcagt gatagagact tataagttcc ctatcagtga tagagacacc
      181 gggatatgag ctgtataaga aaactcgagt tttcttatac agctcatatc tttttaattc
      241 tcgacctcga gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg
      301 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
      361 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat
      421 ccactttggc cgcggctcga gggggttggg gttgcgcctt ttccaaggca gccctgggtt
      481 tgcgcaggga cgcggctgct ctgggcgtgg ttccgggaaa cgcagcggcg ccgaccctgg
      541 gtctcgcaca ttcttcacgt ccgttcgcag cgtcacccgg atcttcgccg ctacccttgt
      601 gggccccccg gcgacgcttc ctgctccgcc cctaagtcgg gaaggttcct tgcggttcgc
      661 ggcgtgccgg acgtgacaaa cggaagccgc acgtctcact agtaccctcg cagacggaca
      721 gcgccaggga gcaatggcag cgcgccgacc gcgatgggct gtggccaata gcggctgctc
      781 agcagggcgc gccgagagca gcggccggga aggggcggtg cgggaggcgg ggtgtggggc
      841 ggtagtgtgg gccctgttcc tgcccgcgcg gtgttccgca ttctgcaagc ctccggagcg
      901 cacgtcggca gtcggctccc tcgttgaccg aatcaccgac ctctctcccc agggggatct
      961 gtgagtttgg ggacccttga ttgttctttc tttttcgcta ttgtaaaatt catgttatat
     1021 ggagggggca aagttttcag ggtgttgttt agaatgggaa gatgtccctt gtatcaccat
     1081 ggaccctcat gataattttg tttctttcac tttctactct gttgacaacc attgtctcct
     1141 cttattttct tttcattttc tgtaactttt tcgttaaact ttagcttgca tttgtaacga
     1201 atttttaaat tcacttttgt ttatttgtca gattgtaagt actttctcta atcacttttt
     1261 tttcaaggca atcagggtat attatattgt acttcagcac agttttagag aacaattgtt
     1321 ataattaaat gataaggtag aatatttctg catataaatt ctggctggcg tggaaatatt
     1381 cttattggta gaaacaacta catcctggtc atcatcctgc ctttctcttt atggttacaa
     1441 tgatatacac tgtttgagat gaggataaaa tactctgagt ccaaaccggg cccctctgct
     1501 aaccatgttc atgccttctt ctttttccta cagctcctgg gcaacgtgct ggttattgtg
     1561 ctgtctcatc attttggcaa agaattgtaa tacgactcac tatagggcga attgatatgt
     1621 ctagattaga taaaagtaaa gtgattaaca gcgcattaga gctgcttaat gaggtcggaa
     1681 tcgaaggttt aacaacccgt aaactcgccc agaagctagg tgtagagcag cctacattgt
     1741 attggcatgt aaaaaataag cgggctttgc tcgacgcctt agccattgag atgttagata
     1801 ggcaccatac tcacttttgc cctttagaag gggaaagctg gcaagatttt ttacgtaata
     1861 acgctaaaag ttttagatgt gctttactaa gtcatcgcga tggagcaaaa gtacatttag
     1921 gtacacggcc tacagaaaaa cagtatgaaa ctctcgaaaa tcaattagcc tttttatgcc
     1981 aacaaggttt ttcactagag aatgcattat atgcactcag cgctgtgggg cattttactt
     2041 taggttgcgt attggaagat caagagcatc aagtcgctaa agaagaaagg gaaacaccta
     2101 ctactgatag tatgccgcca ttattacgac aagctatcga attatttgat caccaaggtg
     2161 cagagccagc cttcttattc ggccttgaat tgatcatatg cggattagaa aaacaactta
     2221 aatgtgaaag tgggtccgcg tacagcggct cccgggagtt ctagggatct gcccctctcc
     2281 ctcccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt
     2341 ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg
     2401 ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg
     2461 tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc
     2521 tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca
     2581 aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag
     2641 ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa
     2701 ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt
     2761 tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt
     2821 tttcctttga aaaacacgat gataaggatc caccggagct taccatgacc gagtacaagc
     2881 ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg
     2941 cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg
     3001 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg
     3061 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg
     3121 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc
     3181 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg
     3241 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc
     3301 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc
     3361 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg
     3421 aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgacgcccg ccccacgacc
     3481 cgcagcgccc gaccgaaagg agcgcacgac cccatgcatc ggtaccttta agaccaatga
     3541 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     3601 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
     3661 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     3721 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     3781 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc
     3841 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact
     3901 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     3961 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     4021 atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     4081 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     4141 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac
     4201 gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa
     4261 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     4321 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     4381 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     4441 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     4501 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     4561 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     4621 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     4681 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     4741 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     4801 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg
     4861 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     4921 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     4981 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     5041 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     5101 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     5161 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     5221 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     5281 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     5341 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     5401 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     5461 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     5521 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     5581 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     5641 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     5701 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     5761 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     5821 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     5881 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     5941 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     6001 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     6061 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     6121 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     6181 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     6241 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     6301 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     6361 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     6421 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     6481 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     6541 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     6601 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     6661 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     6721 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     6781 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     6841 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     6901 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     6961 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
     7021 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct taatgtagtc
     7081 ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca
     7141 aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg atcgtgcctt
     7201 attaggaagg caacagacgg gtctgacatg gattggacga accactgaat tgccgcattg
     7261 cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct ggttagacca
     7321 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     7381 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     7441 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
     7501 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
     7561 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
     7621 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
     7681 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
     7741 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
     7801 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
     7861 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
     7921 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
     7981 gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag
     8041 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
     8101 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
     8161 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
     8221 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
     8281 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
     8341 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
     8401 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
     8461 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
     8521 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
     8581 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
     8641 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
     8701 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
     8761 cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtgga
//
