LOCUS       sequence-210761-        7962 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length BMR1_01g01510 
            ectodomain with no N-linked glycans upon cotransfection with BirA in
            mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7962)
  AUTHORS   Elton CM, Rodriguez M, Ben Mamoun C, Lobo CA, Wright GJ
  TITLE     A library of recombinant Babesia microti cell surface and secreted 
            proteins for diagnostics discovery and reverse vaccinology.
  JOURNAL   Int J Parasitol. 2019 Feb;49(2):115-125. doi: 
            10.1016/j.ijpara.2018.10.003. Epub 2018 Oct 24.
  PUBMED    30367868
REFERENCE   2  (bases 1 to 7962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ijpara.2018.10.003"; journalName: "Int J Parasitol"; 
            date: "2019-02"; volume: "49"; issue: "2"; pages: "115-125"
COMMENT     Sequence Label: BMR1_01g01510-bio-His
FEATURES             Location/Qualifiers
     source          1..7962
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3126..3143
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    3195..3250
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3249..3268)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5486..5504)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5572..5676
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5677..5745,5746..6537)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5895..5914)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6708..7296
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7197..7216
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7450..7467
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7584..7605
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7620..7625,7626..7643,7644..7650)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7658..7674
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7663..7685
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7682..7698
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7682..7698
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgcca
     1201 gcagccctag ctactgtgat gcccccagcg gcttcaccgt gatcaacgtg gccagcctgc
     1261 tggacggcac ccccctgcct atcggcatca ccctggaaac cgacaagagc ggctacaact
     1321 acttcaccgt ggaccggacc ctgccccagt acaagaaagt gggcttctgc ctgaacggct
     1381 acctggacac cttcgacacc cccaaggtgc agaccatcct gcacaagaaa ttcgaggaca
     1441 gcgtgtacgt gtcctacatc gacaagttcg tgaccaacgt ggacggcaac gcccccatct
     1501 gcaccctgac ccacgcctac agcctgtacg gcggcgacat caccagcgcc ctgcctccat
     1561 tcaccgtgcg gaagatcagc gacggcttcc tgagccggct gaacgagcgg aagcagctga
     1621 gcagcatctg cagcaccgcc tgtggcggcc tggtggacgt cgtgggcggc aactacccca
     1681 agggcagcga catcgtgccc cacagcgaga gcgtggacaa agtgaagttc agcgacgtgc
     1741 tggtcaagag cctgaccctg aagtgcggcg accacacctt ctacaacttc gaccgggacc
     1801 agttcgccta catgtacctg ttcgagcacg acggcgccaa atacgccgcc ctgagcagac
     1861 tgacccccac catgatcgag gtggccgtga tgtgcaaata cggcaacggc gtggaagaga
     1921 agctgctgta cgacaacgac gccgagttcc agcaggccgt gctgaagctg gtgcccatca
     1981 acatcttcat cgagaacacc gccagcatgc cccacggcgc cagcatgacc aagctggacg
     2041 agaacatcga cgagatcgcc atcaacagat tcgtgctgag atacccctac gtgctgctgc
     2101 ccggcctggc cgtggacatc ggcaagggca acgccgacac catctggatc gtgaagccca
     2161 gcgaagagga cagcgaagtg aagtacttcg tggtgttcgg cgtgaagggc gagatcgaca
     2221 gcgtggacga catcaagttc atcagcgcct actacgtgaa caaagagaac gtcgccgtca
     2281 atatcgccaa ccggttcatc ctgggcccca acattgccag agccagcacc cccgtgtaca
     2341 gccactacag caagctgtac ccccccagat acaagatcga ctacaagttc tccggcgtgt
     2401 tcagccccct gagctacgtg gtggccggca acagccaggg ctccgacgac gtgtccgtgg
     2461 aaaaggccgt ggctggcgcg ccgtcgacct ccatcacggc ctataagagt gagggggagt
     2521 cagcggagtt ctccttccca ctcaaccttg gagaggaaag cctgcaggga gagttgagat
     2581 ggaaggcaga gaaggctcct tcttcccagt cctggatcac cttctcccta aagaaccaaa
     2641 aggtgtctgt gcagaagtct actagcaacc ccaagttcca gctgtccgaa acgctcccac
     2701 tcacccttca gataccccag gtctcccttc agtttgctgg ttctggcaac ctgaccctga
     2761 ctctggacag agggatactg tatcaggaag tgaacctggt ggtgatgaaa gtgactcagc
     2821 ccgacagcaa cactttgacc tgtgaggtga tgggacccac ctcacccaag atgagactga
     2881 tcttgaagca ggagaatcag gaggccaggg tctccaggca ggagaaagtg attcaagtgc
     2941 aggcccctga agcaggggtg tggcaatgtc tactgagtga aggtgaagag gtcaagatgg
     3001 actccaagat ccaggtttta tccaaagggt tgaattccgg cagcctgcac cacatcctgg
     3061 acgcccagaa gatgctgtgg aaccacaggg acaggaacct gcctcctctg gcccctctgg
     3121 gcccccacca ccatcaccac cattgagtct agacacgtga ttaattaagg atcccccgac
     3181 ctcgacctct ggctaataaa ggaaatttat tttcattgca atagtgtgtt ggaatttttt
     3241 gtgtctctca ctcggaagga catatgggag ggcaaatcat ttggtcgaga tccctcggag
     3301 atctctagct agaggatcga tccccgcccc ggacgaacta aacctgacta cgacatctct
     3361 gccccttctt cgcggggcag tgcatgtaat cccttcagtt ggttggtaca acttgccaac
     3421 tgggccctgt tccacatgtg acacgggggg ggaccaaaca caaaggggtt ctctgactgt
     3481 agttgacatc cttataaatg gatgtgcaca tttgccaaca ctgagtggct ttcatcctgg
     3541 agcagacttt gcagtctgtg gactgcaaca caacattgcc tttatgtgta actcttggct
     3601 gaagctctta caccaatgct gggggacatg tacctcccag gggcccagga agactacggg
     3661 aggctacacc aacgtcaatc agaggggcct gtgtagctac cgataagcgg accctcaaga
     3721 gggcattagc aatagtgttt ataaggcccc cttgttaacc ctaaacgggt agcatatgct
     3781 tcccgggtag tagtatatac tatccagact aaccctaatt caatagcata tgttacccaa
     3841 cgggaagcat atgctatcga attagggtta gtaaaagggt cctaaggaac agcgatatct
     3901 cccaccccat gagctgtcac ggttttattt acatggggtc aggattccac gagggtagtg
     3961 aaccatttta gtcacaaggg cagtggctga agatcaagga gcgggcagtg aactctcctg
     4021 aatcttcgcc tgcttcttca ttctccttcg tttagctaat agaataactg ctgagttgtg
     4081 aacagtaagg tgtatgtgag gtgctcgaaa acaaggtttc aggtgacgcc cccagaataa
     4141 aatttggacg gggggttcag tggtggcatt gtgctatgac accaatataa ccctcacaaa
     4201 ccccttgggc aataaatact agtgtaggaa tgaaacattc tgaatatctt taacaataga
     4261 aatccatggg gtggggacaa gccgtaaaga ctggatgtcc atctcacacg aatttatggc
     4321 tatgggcaac acataatcct agtgcaatat gatactgggg ttattaagat gtgtcccagg
     4381 cagggaccaa gacaggtgaa ccatgttgtt acactctatt tgtaacaagg ggaaagagag
     4441 tggacgccga cagcagcgga ctccactggt tgtctctaac acccccgaaa attaaacggg
     4501 gctccacgcc aatggggccc ataaacaaag acaagtggcc actctttttt ttgaaattgt
     4561 ggagtggggg cacgcgtcag cccccacacg ccgccctgcg gttttggact gtaaaataag
     4621 ggtgtaataa cttggctgat tgtaaccccg ctaaccactg cggtcaaacc acttgcccac
     4681 aaaaccacta atggcacccc ggggaatacc tgcataagta ggtgggcggg ccaagatagg
     4741 ggcgcgattg ctgcgatctg gaggacaaat tacacacact tgcgcctgag cgccaagcac
     4801 agggttgttg gtcctcatat tcacgaggtc gctgagagca cggtgggcta atgttgccat
     4861 gggtagcata tactacccaa atatctggat agcatatgct atcctaatct atatctgggt
     4921 agcataggct atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt
     4981 agtatatgct atcctaattt atatctgggt agcataggct atcctaatct atatctgggt
     5041 agcatatgct atcctaatct atatctgggt agtatatgct atcctaatct gtatccgggt
     5101 agcatatgct atcctaatag agattagggt agtatatgct atcctaattt atatctgggt
     5161 agcatatact acccaaatat ctggatagca tatgctatcc taatctatat ctgggtagca
     5221 tatgctatcc taatctatat ctgggtagca taggctatcc taatctatat ctgggtagca
     5281 tatgctatcc taatctatat ctgggtagta tatgctatcc taatttatat ctgggtagca
     5341 taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta
     5401 tatgctatcc taatctgtat ccgggtagca tatgctatcc tcatgataag ctgtcaaaca
     5461 tgagaatttt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     5521 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     5581 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     5641 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     5701 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     5761 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     5821 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     5881 acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa
     5941 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     6001 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     6061 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     6121 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     6181 gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg
     6241 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     6301 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     6361 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     6421 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     6481 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     6541 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     6601 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     6661 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     6721 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     6781 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     6841 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     6901 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     6961 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     7021 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     7081 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     7141 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     7201 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     7261 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     7321 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     7381 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     7441 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     7501 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     7561 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     7621 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     7681 acaggaaaca gctatgacca tgattacgcc aagctctagc tagaggtcga ccaattctca
     7741 tgtttgacag cttatcatcg cagatccggg caacgttgtt gccattgctg caggcgcaga
     7801 actggtaggt atggaagatc tatacattga atcaatattg gcaattagcc atattagtca
     7861 ttggttatat agcataaatc aatattggct attggccatt gcatacgttg tatctatatc
     7921 ataatatgta catttatatt ggctcatgtc caatatgacc gc
//
