LOCUS       sequence-210762-        5742 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 505 / 569, Emission: 516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5742)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 5742)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos4a-Actin-C-18
FEATURES             Location/Qualifiers
     source          1..5742
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        119..422
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        423..626
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     577..597
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      665..674
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             671..1351
                     /codon_start=1
                     /product="fixation-resistant green-to-red photoswitchable 
                     fluorescent protein (Paez-Segala et al., 2015)"
                     /label=mEos4a
                     /note="derived from mEos2"
                     /translation="MVSAIKPDMRIKLRMEGNVNGHHFVIDGDGTGKPYEGKQTMDLEV
                     KEGGPLPFAFDILTTAFHYGNRVFVKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICYA
                     RNDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALL
                     LEGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHAIEILSHDKDYNKVKLYEHAVAHSGLP
                     DNARR"
     polyA_signal    2660..2781
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2697..2716)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2751..2770
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2788..3243)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2925..2946)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3137..3156
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3270..3374
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3399..3419)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3443..3653
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        3465..3661
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3512..3647
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3696..4490
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3696..4490
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3750..3769)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4360..4379
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4678..4697)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4722..4769
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5098..5686
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5587..5606
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcatta
       61 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      121 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      181 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      241 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      301 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      361 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      421 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
      481 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      541 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
      601 ggtgggaggt ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc
      661 ggtcgccacc atggtgagtg cgattaagcc agacatgagg atcaaactcc gtatggaagg
      721 caacgtaaac gggcaccact ttgtgatcga cggagatggt acaggcaagc cttatgaggg
      781 aaaacagacc atggatcttg aagtcaaaga gggcggacct ctgccttttg cctttgatat
      841 cctgaccact gcattccatt acggcaacag ggtattcgtt aaatatccag acaacataca
      901 agactatttt aagcagtcgt ttcctaaggg gtattcgtgg gaacgaagct tgactttcga
      961 agacgggggc atttgctatg ccagaaacga cataacaatg gaaggggaca ctttctataa
     1021 taaagttcga ttttatggta ccaactttcc cgccaatggt ccagttatgc agaagaagac
     1081 gctgaaatgg gagccctcca ctgagaaaat gtatgtgcgt gatggagtgc tgacgggtga
     1141 tattcatatg gctttgttgc ttgaaggaaa tgcccattac cgatgtgact tcagaactac
     1201 ttacaaagct aaggagaagg gtgtcaagtt accaggctac cactttgtgg accacgccat
     1261 tgagatttta agccatgaca aagattacaa caaggttaag ctgtatgagc atgctgttgc
     1321 tcattctgga ttgcctgaca atgccagacg atccggactc agatctggca gcggtggagg
     1381 cagcgcatcc ggcggaagcg gaagcgatga tgatatcgcc gcgctcgtcg tcgacaacgg
     1441 ctccggcatg tgcaaggccg gcttcgcggg cgacgatgcc ccccgggccg tcttcccctc
     1501 catcgtgggg cgccccaggc accagggcgt gatggtgggc atgggtcaga aggattccta
     1561 tgtgggcgac gaggcccaga gcaagagagg catcctcacc ctgaagtacc ccatcgagca
     1621 cggcatcgtc accaactggg acgacatgga gaaaatctgg caccacacct tctacaatga
     1681 gctgcgtgtg gctcccgagg agcaccccgt gctgctgacc gaggcccccc tgaaccccaa
     1741 ggccaaccgc gagaagatga cccagatcat gtttgagacc ttcaacaccc cagccatgta
     1801 cgttgctatc caggctgtgc tatccctgta cgcctctggc cgtaccactg gcatcgtgat
     1861 ggactccggt gacggggtca cccacactgt gcccatctac gaggggtatg ccctccccca
     1921 tgccatcctg cgtctggacc tggctggccg ggacctgact gactacctca tgaagatcct
     1981 caccgagcgc ggctacagct tcaccaccac ggccgagcgg gaaatcgtgc gtgacattaa
     2041 ggagaagctg tgctacgtcg ccctggactt cgagcaagag atggccacgg ctgcttccag
     2101 ctcctccctg gagaagagct acgagctgcc tgacggccag gtcatcacca ttggcaatga
     2161 gcggttccgc tgccctgagg cactcttcca gccttccttc ctgggcatgg agtcctgtgg
     2221 catccacgaa actaccttca actccatcat gaagtgtgac gtggacatcc gcaaagacct
     2281 gtacgccaac acagtgctgt ctggcggcac caccatgtac cctggcattg ccgacaggat
     2341 gcagaaggag atcactgccc tggcacccag cacaatgaag atcaagatca ttgctcctcc
     2401 tgagcgcaag tactccgtgt ggatcggcgg ctccatcctg gcctcgctgt ccaccttcca
     2461 gcagatgtgg atcagcaagc aggagtatga cgagtccggc ccctccatcg tccaccgcaa
     2521 atgcttctag ggatccaccg gatctagata actgatcata atcagccata ccacatttgt
     2581 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
     2641 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
     2701 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     2761 caaactcatc aatgtatctt aaggcgtaaa ttgtaagcgt taatattttg ttaaaattcg
     2821 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
     2881 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
     2941 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
     3001 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag
     3061 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
     3121 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
     3181 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
     3241 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     3301 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     3361 gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg
     3421 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     3481 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     3541 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     3601 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     3661 agatcgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg
     3721 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
     3781 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
     3841 tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt
     3901 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
     3961 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
     4021 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
     4081 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
     4141 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
     4201 aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg
     4261 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     4321 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
     4381 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     4441 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
     4501 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac
     4561 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
     4621 cctccagcgc ggggatctca tgctggagtt cttcgcccac cctaggggga ggctaactga
     4681 aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa
     4741 taaaacgcac ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca
     4801 ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc
     4861 cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc
     4921 aggccctgcc atagcctcag gttactcata tatactttag attgatttaa aacttcattt
     4981 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     5041 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     5101 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     5161 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     5221 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     5281 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     5341 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     5401 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     5461 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     5521 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     5581 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     5641 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     5701 ggccttttta cggttcctgg ccttttgctg gccttttgct ca
//
