LOCUS       sequence-210931-       14560 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14560)
  AUTHORS   Tasic B, Miyamichi K, Hippenmeyer S, Dani VS, Zeng H, Joo W, Zong H,
            Chen-Tsai Y, Luo L
  TITLE     Extensions of MADM (Mosaic Analysis with Double Markers) in Mice.
  JOURNAL   PLoS One. 2012;7(3):e33332. Epub 2012 Mar 27.
  PUBMED    22479386
REFERENCE   2  (bases 1 to 14560)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(3)"; pages: "e33332. Epub 2012 Mar 27"
COMMENT     Sequence Label: pRosa26-TG
FEATURES             Location/Qualifiers
     source          1..14560
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    3..1085
                     /label=Rosa26 left arm
                     /note="left homology arm from intron 1 of the mouse Rosa26 
                     gene (Soriano, 1999)"
     enhancer        1181..1484
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1486..1763
                     /label=chicken beta-actin promoter
     intron          1764..2780
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2704..2726
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2788..2807
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      2859..2868
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    2887..2920
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    complement(2921..2954)
                     /label=lox5171
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTGTAC) (Shaw et al., 2021). lox5171 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(2955..2988)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    2997..3030
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    complement(3037..3070)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        3098..3202
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3204..3561
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3204..3395
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3227..3247)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3412..3547
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3596..4390
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3596..4390
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3650..3669)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4260..4279
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4578..4597)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4622..4669
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     protein_bind    complement(4855..4888)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(5040..5059)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5387..5408
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    5581..5702
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5618..5637)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5672..5691
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     misc_feature    5722..10062
                     /label=Rosa26 right arm
                     /note="right homology arm from intron 1 of the mouse Rosa26
                     gene (Soriano, 1999)"
     promoter        10112..10611
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(10147..10170)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     10544..10563
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             10638..11210
                     /codon_start=1
                     /gene="Corynebacterium diphtheriae tox"
                     /product="diphtheria toxin fragment A"
                     /label=DTA
                     /translation="DDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYD
                     DDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKEL
                     GLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEI
                     NFETRGKRGQDAMYEYMAQACAGNRVRR"
     intron          11287..11352
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     primer_bind     complement(11420..11437)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    11426..11650
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(11712..11732)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(11712..11730)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11751..11767)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11751..11767)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11764..11786)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11775..11791
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11799..11805,11806..11823,11824..11829))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11844..11865
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11982..11999)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12153..12741)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12233..12252)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12912..13703,13704..13772))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13535..13554
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13773..13877)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(13903..14358)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(14040..14061)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     14252..14271
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     14485..14507
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     14499..14516
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     14500..14516
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     14526..14545
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        14526..14544
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ccgcggcagg ccctccgagc gtggtggagc cgttctgtga gacagccggg tacgagtcgt
       61 gacgctggaa ggggcaagcg ggtggtgggc aggaatgcgg tccgccctgc agcaaccgga
      121 gggggaggga gaagggagcg gaaaagtctc caccggacgc ggccatggct cggggggggg
      181 ggggcagcgg aggagcgctt ccggccgacg tctcgtcgct gattggcttc ttttcctccc
      241 gccgtgtgtg aaaacacaaa tggcgtgttt tggttggcgt aaggcgcctg tcagttaacg
      301 gcagccggag tgcgcagccg ccggcagcct cgctctgccc actgggtggg gcgggaggta
      361 ggtggggtga ggcgagctgg acgtgcgggc gcggtcggcc tctggcgggg cggggggggg
      421 gagggagggt cagcgaaagt agctcgcgcg cgagcggccg cccaccctcc ccttcctctg
      481 ggggagtcgt tttacccgcc gccggccggg cctcgtcgtc tgattggctc tcggggccca
      541 gaaaactggc ccttgccatt ggctcgtgtt cgtgcaagtt gagtccatcc gccggccagc
      601 gggggcggcg aggaggcgct cccaggttcc ggccctcccc tcggccccgc gccgcagagt
      661 ctggccgcgc gcccctgcgc aacgtggcag gaagcgcgcg ctgggggcgg ggacgggcag
      721 tagggctgag cggctgcggg gcgggtgcaa gcacgtttcc gacttgagtt gcctcaagag
      781 gggcgtgctg agccagacct ccatcgcgca ctccggggag tggagggaag gagcgagggc
      841 tcagttgggc tgttttggag gcaggaagca cttgctctcc caaagtcgct ctgagttgtt
      901 atcagtaagg gagctgcagt ggagtaggcg gggagaaggc cgcacccttc tccggagggg
      961 ggaggggagt gttgcaatac ctttctggga gttctctgct gcctcctggc ttctgaggac
     1021 cgccctgggc ctgggagaat cccttccccc tcttccctcg tgatctgcaa ctccagtctt
     1081 tctagcctta attaaccgtt taaacaattc tgcaggaatc tagttattaa tagtaatcaa
     1141 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     1201 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     1261 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     1321 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     1381 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     1441 ctacttggca gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca
     1501 cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg tatttattta
     1561 ttttttaatt attttgtgca gcgatggggg cggggggggg gggggggcgc gcgccaggcg
     1621 gggcgggggg gggggggggg gggggggggg cggggcggag aggtgcggcg gcagccaatc
     1681 agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata
     1741 aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgcgctg ccttcgcccc gtgccccgct
     1801 ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac cgcgttactc ccacaggtga
     1861 gcgggcggga cggcccttct cctccgggct gtaattagcg cttggtttaa tgacggcttg
     1921 tttcttttct gtggctgcgt gaaagccttg aggggctccg ggagggccct ttgtgcgggg
     1981 ggagcggctc ggggggtgcg tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg
     2041 cgctgcccgg cggctgtgag cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg
     2101 tgcgcgaggg gagcgcggcc gggggcggtg ccccgcggtg cggggggggc tgcgagggga
     2161 acaaaggctg cgtgcggggt gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg
     2221 tcgggctgca accccccctg cacccccctc cccgagttgc tgagcacggc ccggcttcgg
     2281 gtgcggggct ccgtacgggg cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc
     2341 aggtgggggt gccgggcggg gcggggccgc ctcgggccgg ggagggctcg ggggaggggc
     2401 gcggcggccc ccggagcgcc ggcggctgtc gaggcgcggc gagccgcagc cattgccttt
     2461 tatggtaatc gtgcgagagg gcgcagggac ttcctttgtc ccaaatctgt gcggagccga
     2521 aatctgggag gcgccgccgc accccctcta gcgggcgcgg ggcgaagcgg tgcggcgccg
     2581 gcaggaagga aatgggcggg gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc
     2641 ctctccagcc tcggggctgt ccgcgggggg acggctgcct tcggggggga cggggcaggg
     2701 cggggttcgg cttctggcgt gtgaccggcg gctctagagc ctctgctaac catgttcatg
     2761 ccttcttctt tttcctacag ctcctgggca acgtgctggt tattgtgctg tctcatcatt
     2821 ttggcaaaga attgatttga taccgcgggc ccgggttcgc caccatggtt ggtatcaaag
     2881 atctccgaag ttcctattct ctagaaagta taggaacttc ataacttcgt atagtacaca
     2941 ttatacgaag ttatataact tcgtatagga tactttatac gaagttatgg atctccgaag
     3001 ttcctattct ctagaaagta taggaacttc ggatctataa cttcgtatag catacattat
     3061 acgaagttat tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     3121 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     3181 ataatattga aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag
     3241 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     3301 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     3361 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     3421 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     3481 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     3541 ggaggcctag gcttttgcaa agatcgatca agagacagga tgaggatcgt ttcgcatgat
     3601 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
     3661 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
     3721 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga
     3781 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
     3841 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
     3901 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
     3961 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
     4021 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
     4081 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga
     4141 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
     4201 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
     4261 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     4321 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     4381 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca
     4441 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     4501 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     4561 cctaggggga ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg
     4621 acggcaataa aaagacagaa taaaacgcac ggtgttgggt cgtttgttca taaacgcggg
     4681 gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac
     4741 gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc
     4801 gcagccaacg tcggggcggc aggccctgcc atagcctcag gttactcagg atctataact
     4861 tcgtatagca tacattatac gaagttatgg ttacaagaca ggtttaagga gaccaataga
     4921 aactgggcat gtggagacag agaagactct tgggtttctg ataggcactg actctctctg
     4981 cctattggtc tattttccca cccttagggt acgtccagga gcgcaccatc ttcttcaagg
     5041 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     5101 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     5161 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
     5221 aggcgaactt caagatccgc cacaacatcg aggacggcgg cgtgcagctc gccgaccact
     5281 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     5341 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     5401 agttcgtgac cgccgccggg atcactctcg gcatggacga gctttacaag gatatccctg
     5461 aattctagat aactgatcat aatcagccat accacatttg tagaggtttt acttgcttta
     5521 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
     5581 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     5641 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     5701 tatttaaatt gggcgcgccg gctagaagat gggcgggagt cttctgggca ggcttaaagg
     5761 ctaacctggt gtgtgggcgt tgtcctgcag gggaattgaa caggtgtaaa attggaggga
     5821 caagacttcc cacagatttt cggttttgtc gggaagtttt ttaatagggg caaataagga
     5881 aaatgggagg ataggtagtc atctggggtt ttatgcagca aaactacagg ttattattgc
     5941 ttgtgatccg cctcggagta ttttccatcg aggtagatta aagacatgct cacccgagtt
     6001 ttatactctc ctgcttgaga tccttactac agtatgaaat tacagtgtcg cgagttagac
     6061 tatgtaagca gaattttaat catttttaaa gagcccagta cttcatatcc atttctcccg
     6121 ctccttctgc agccttatca aaaggtattt tagaacactc attttagccc cattttcatt
     6181 tattatactg gcttatccaa cccctagaca gagcattggc attttccctt tcctgatctt
     6241 agaagtctga tgactcatga aaccagacag attagttaca tacaccacaa atcgaggctg
     6301 tagctggggc ctcaacactg cagttctttt ataactcctt agtacacttt ttgttgatcc
     6361 tttgccttga tccttaattt tcagtgtcta tcacctctcc cgtcaggtgg tgttccacat
     6421 ttgggcctat tctcagtcca gggagtttta caacaataga tgtattgaga atccaaccta
     6481 aagcttaact ttccactccc atgaatgcct ctctcctttt tctccattta taaactgagc
     6541 tattaaccat taatggtttc caggtggatg tctcctcccc caatattacc tgatgtatct
     6601 tacatattgc caggctgata ttttaagaca ttaaaaggta tatttcatta ttgagccaca
     6661 tggtattgat tactgcttac taaaattttg tcattgtaca catctgtaaa aggtggttcc
     6721 ttttggaatg caaagttcag gtgtttgttg tctttcctga cctaaggtct tgtgagcttg
     6781 tattttttct atttaagcag tgctttctct tggactggct tgactcatgg cattctacac
     6841 gttattgctg gtctaaatgt gattttgcca agcttcttca ggacctataa ttttgcttga
     6901 cttgtagcca aacacaagta aaatgattaa gcaacaaatg tatttgtgaa gcttggtttt
     6961 taggttgttg tgttgtgtgt gcttgtgctc tataataata ctatccaggg gctggagagg
     7021 tggctcggag ttcaagagca cagactgctc ttccagaagt cctgagttca attcccagca
     7081 accacatggt ggctcacaac catctgtaat gggatctgat gccctcttct ggtgtgtctg
     7141 aagaccacaa gtgtattcac attaaataaa taaatcctcc ttcttcttct tttttttttt
     7201 tttaaagaga atactgtctc cagtagaatt tactgaagta atgaaatact ttgtgtttgt
     7261 tccaatatgg tagccaataa tcaaattact ctttaagcac tggaaatgtt accaaggaac
     7321 taatttttat ttgaagtgta actgtggaca gaggagccat aactgcagac ttgtgggata
     7381 cagaagacca atgcagactt taatgtcttt tctcttacac taagcaataa agaaataaaa
     7441 attgaacttc tagtatccta tttgtttaaa ctgctagctt tacttaactt ttgtgcttca
     7501 tctatacaaa gctgaaagct aagtctgcag ccattactaa acatgaaagc aagtaatgat
     7561 aattttggat ttcaaaaatg tagggccaga gtttagccag ccagtggtgg tgcttgcctt
     7621 tatgccttta atcccagcac tctggaggca gagacaggca gatctctgag tttgagccca
     7681 gcctggtcta cacatcaagt tctatctagg atagccagga atacacacag aaaccctgtt
     7741 ggggaggggg gctctgagat ttcataaaat tataattgaa gcattcccta atgagccact
     7801 atggatgtgg ctaaatccgt ctacctttct gatgagattt gggtattatt ttttctgtct
     7861 ctgctgttgg ttgggtcttt tgacactgtg ggctttcttt aaagcctcct tcctgccatg
     7921 tggtctcttg tttgctacta acttcccatg gcttaaatgg catggctttt tgccttctaa
     7981 gggcagctgc tgagatttgc agcctgattt ccagggtggg gttgggaaat ctttcaaaca
     8041 ctaaaattgt cctttaattt tttttttaaa aaatgggtta tataataaac ctcataaaat
     8101 agttatgagg agtgaggtgg actaatatta aatgagtccc tcccctataa aagagctatt
     8161 aaggcttttt gtcttatact taactttttt tttaaatgtg gtatctttag aaccaagggt
     8221 cttagagttt tagtatacag aaactgttgc atcgcttaat cagattttct agtttcaaat
     8281 ccagagaatc caaattcttc acagccaaag tcaaattaag aatttctgac ttttaatgtt
     8341 aatttgctta ctgtgaatat aaaaatgata gcttttcctg aggcagggtc tcactatgta
     8401 tctctgcctg atctgcaaca agatatgtag actaaagttc tgcctgcttt tgtctcctga
     8461 atactaaggt taaaatgtag taatactttt ggaacttgca ggtcagattc ttttataggg
     8521 gacacactaa gggagcttgg gtgatagttg gtaaaatgtg tttcaagtga tgaaaacttg
     8581 aattattatc accgcaacct actttttaaa aaaaaaagcc aggcctgtta gagcatgctt
     8641 aagggatccc taggacttgc tgagcacaca agagtagtta cttggcaggc tcctggtgag
     8701 agcatatttc aaaaaacaag gcagacaacc aagaaactac agttaaggtt acctgtcttt
     8761 aaaccatctg catatacaca gggatattaa aatattccaa ataatatttc attcaagttt
     8821 tcccccatca aattgggaca tggatttctc cggtgaatag gcagagttgg aaactaaaca
     8881 aatgttggtt ttgtgatttg tgaaattgtt ttcaagtgat agttaaagcc catgagatac
     8941 agaacaaagc tgctatttcg aggtctcttg gtttatactc agaagcactt ctttgggttt
     9001 ccctgcacta tcctgatcat gtgctaggcc taccttaggc tgattgttgt tcaaataaac
     9061 ttaagtttcc tgtcaggtga tgtcatatga tttcatatat caaggcaaaa catgttatat
     9121 atgttaaaca tttgtactta atgtgaaagt taggtctttg tgggtttgat ttttaatttt
     9181 caaaacctga gctaaataag tcatttttac atgtcttaca tttggtggaa ttgtataatt
     9241 gtggtttgca ggcaagactc tctgacctag taaccctacc tatagagcac tttgctgggt
     9301 cacaagtcta ggagtcaagc atttcacctt gaagttgaga cgttttgtta gtgtatacta
     9361 gtttatatgt tggaggacat gtttatccag aagatattca ggactatttt tgactgggct
     9421 aaggaattga ttctgattag cactgttagt gagcattgag tggcctttag gcttgaattg
     9481 gagtcacttg tatatctcaa ataatgctgg ccttttttaa aaagcccttg ttctttatca
     9541 ccctgttttc tacataattt ttgttcaaag aaatacttgt ttggatctcc ttttgacaac
     9601 aatagcatgt tttcaagcca tatttttttt cctttttttt tttttttttg gtttttcgag
     9661 acagggtttc tctgtatagc cctggctgtc ctggaactca ctttgtagac caggctggcc
     9721 tcgaactcag aaatccgcct gcctctgcct cctgagtgcc gggattaaag gcgtgcacca
     9781 ccacgcctgg ctaagttgga tattttgtta tataactata accaatacta actccactgg
     9841 gtggattttt aattcagtca gtagtcttaa gtggtcttta ttggcccttc attaaaatct
     9901 actgttcact ctaacagagg ctgttggtac tagtggcact taagcaactt cctacggata
     9961 tactagcaga ttaagggtca gggatagaaa ctagtctagc gttttgtata cctaccagct
    10021 ttatactacc ttgttctgat agaaatattt caggacatct agcttatcga taccgtcgac
    10081 ggtatcgata agcttgatat cgaattctac cgggtagggg aggcgctttt cccaaggcag
    10141 tctggagcat gcgctttagc agccccgctg ggcacttggc gctacacaag tggcctctgg
    10201 cctcgcacac attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc
    10261 ttcgcgccac cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg
    10321 tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc
    10381 accgctgagc aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc
    10441 ttcgctttct gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg
    10501 ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa
    10561 aagcgcacgt ctgccgcgct gttctcctct tcctcatctc cgggcctttc gacctgcagg
    10621 tcctcgccat ggatcctgat gatgttgttg attcttctaa atcttttgtg atggaaaact
    10681 tttcttcgta ccacgggact aaacctggtt atgtagattc cattcaaaaa ggtatacaaa
    10741 agccaaaatc tggtacacaa ggaaattatg acgatgattg gaaagggttt tatagtaccg
    10801 acaataaata cgacgctgcg ggatactctg tagataatga aaacccgctc tctggaaaag
    10861 ctggaggcgt ggtcaaagtg acgtatccag gactgacgaa ggttctcgca ctaaaagtgg
    10921 ataatgccga aactattaag aaagagttag gtttaagtct cactgaaccg ttgatggagc
    10981 aagtcggaac ggaagagttt atcaaaaggt tcggtgatgg tgcttcgcgt gtagtgctca
    11041 gccttccctt cgctgagggg agttctagcg ttgaatatat taataactgg gaacaggcga
    11101 aagcgttaag cgtagaactt gagattaatt ttgaaacccg tggaaaacgt ggccaagatg
    11161 cgatgtatga gtatatggct caagcctgtg caggaaatcg tgtcaggcga tctctttgtg
    11221 aaggaacctt acttctgtgg tgtgacataa ttggacaaac tacctacaga gatttaaagc
    11281 tctaaggtaa atataaaatt tttaagtgta taatgtgtta aactactgat tctaattgtt
    11341 tgtgtatttt agattccaac ctatggaact gatgaatggg agcagtggtg gaatgcagat
    11401 cctagagctc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
    11461 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
    11521 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
    11581 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
    11641 ggctctatgg cttctgaggc ggaaagaacc agctggggct cgaggggggg cccggtaccc
    11701 agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg
    11761 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
    11821 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
    11881 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
    11941 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
    12001 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
    12061 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
    12121 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
    12181 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
    12241 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
    12301 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
    12361 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
    12421 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
    12481 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
    12541 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
    12601 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
    12661 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
    12721 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
    12781 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
    12841 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
    12901 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
    12961 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
    13021 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
    13081 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
    13141 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
    13201 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
    13261 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
    13321 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
    13381 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
    13441 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
    13501 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
    13561 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
    13621 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
    13681 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
    13741 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
    13801 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
    13861 caaatagggg ttccgcgcac atttccccga aaagtgcaat ggaaattgta agcgttaata
    13921 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
    13981 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
    14041 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
    14101 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
    14161 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
    14221 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
    14281 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
    14341 cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg caactgttgg gaagggcgat
    14401 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
    14461 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgagc
    14521 gcgcgtaata cgactcacta tagggcgaat tggagctcca
//
