LOCUS       sequence-210933-       15938 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15938)
  AUTHORS   Tasic B, Miyamichi K, Hippenmeyer S, Dani VS, Zeng H, Joo W, Zong H,
            Chen-Tsai Y, Luo L
  TITLE     Extensions of MADM (Mosaic Analysis with Double Markers) in Mice.
  JOURNAL   PLoS One. 2012;7(3):e33332. Epub 2012 Mar 27.
  PUBMED    22479386
REFERENCE   2  (bases 1 to 15938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(3)"; pages: "e33332. Epub 2012 Mar 27"
COMMENT     Sequence Label: pRosa26-GT
FEATURES             Location/Qualifiers
     source          1..15938
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    3..1085
                     /label=Rosa26 left arm
                     /note="left homology arm from intron 1 of the mouse Rosa26 
                     gene (Soriano, 1999)"
     enhancer        1181..1484
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1486..1763
                     /label=chicken beta-actin promoter
     intron          1764..2780
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2704..2726
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2788..2807
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      2867..2876
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(2918..2939)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     protein_bind    3166..3199
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    complement(3200..3233)
                     /label=lox5171
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTGTAC) (Shaw et al., 2021). lox5171 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(3234..3267)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    3276..3309
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    complement(3316..3349)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        3377..3481
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3483..3840
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3483..3674
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3506..3526)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3691..3826
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3875..4669
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3875..4669
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3929..3948)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4539..4558
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4857..4876)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4901..4948
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     protein_bind    complement(5140..5173)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             5293..6717
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGL
                     VTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIHQ
                     ALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRHH
                     LFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYE
                     GTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMN
                     FEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVL
                     KGEIHQALKLKDGGRYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYER
                     SEGRHHLFLYGMDELYK"
     CDS             6727..6756
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             6763..6792
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             6799..6828
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    6959..7080
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6996..7015)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7050..7069
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     misc_feature    7100..11440
                     /label=Rosa26 right arm
                     /note="right homology arm from intron 1 of the mouse Rosa26
                     gene (Soriano, 1999)"
     promoter        11490..11989
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(11525..11548)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     11922..11941
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             12016..12588
                     /codon_start=1
                     /gene="Corynebacterium diphtheriae tox"
                     /product="diphtheria toxin fragment A"
                     /label=DTA
                     /translation="DDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYD
                     DDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKEL
                     GLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEI
                     NFETRGKRGQDAMYEYMAQACAGNRVRR"
     intron          12665..12730
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     primer_bind     complement(12798..12815)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    12804..13028
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(13090..13110)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(13090..13108)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(13129..13145)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13129..13145)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(13142..13164)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    13153..13169
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(13177..13183,13184..13201,13202..13207))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13222..13243
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(13360..13377)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13531..14119)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13611..13630)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(14290..15081,15082..15150))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14913..14932
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(15151..15255)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(15281..15736)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(15418..15439)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     15630..15649
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     15863..15885
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     15877..15894
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     15878..15894
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     15904..15923
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        15904..15922
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ccgcggcagg ccctccgagc gtggtggagc cgttctgtga gacagccggg tacgagtcgt
       61 gacgctggaa ggggcaagcg ggtggtgggc aggaatgcgg tccgccctgc agcaaccgga
      121 gggggaggga gaagggagcg gaaaagtctc caccggacgc ggccatggct cggggggggg
      181 ggggcagcgg aggagcgctt ccggccgacg tctcgtcgct gattggcttc ttttcctccc
      241 gccgtgtgtg aaaacacaaa tggcgtgttt tggttggcgt aaggcgcctg tcagttaacg
      301 gcagccggag tgcgcagccg ccggcagcct cgctctgccc actgggtggg gcgggaggta
      361 ggtggggtga ggcgagctgg acgtgcgggc gcggtcggcc tctggcgggg cggggggggg
      421 gagggagggt cagcgaaagt agctcgcgcg cgagcggccg cccaccctcc ccttcctctg
      481 ggggagtcgt tttacccgcc gccggccggg cctcgtcgtc tgattggctc tcggggccca
      541 gaaaactggc ccttgccatt ggctcgtgtt cgtgcaagtt gagtccatcc gccggccagc
      601 gggggcggcg aggaggcgct cccaggttcc ggccctcccc tcggccccgc gccgcagagt
      661 ctggccgcgc gcccctgcgc aacgtggcag gaagcgcgcg ctgggggcgg ggacgggcag
      721 tagggctgag cggctgcggg gcgggtgcaa gcacgtttcc gacttgagtt gcctcaagag
      781 gggcgtgctg agccagacct ccatcgcgca ctccggggag tggagggaag gagcgagggc
      841 tcagttgggc tgttttggag gcaggaagca cttgctctcc caaagtcgct ctgagttgtt
      901 atcagtaagg gagctgcagt ggagtaggcg gggagaaggc cgcacccttc tccggagggg
      961 ggaggggagt gttgcaatac ctttctggga gttctctgct gcctcctggc ttctgaggac
     1021 cgccctgggc ctgggagaat cccttccccc tcttccctcg tgatctgcaa ctccagtctt
     1081 tctagcctta attaaccgtt taaacaattc tgcaggaatc tagttattaa tagtaatcaa
     1141 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     1201 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     1261 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     1321 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     1381 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     1441 ctacttggca gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca
     1501 cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg tatttattta
     1561 ttttttaatt attttgtgca gcgatggggg cggggggggg gggggggcgc gcgccaggcg
     1621 gggggggggg gggggggggg gggggggggg cgggggggag aggtgcggcg gcagccaatc
     1681 agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata
     1741 aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgcgctg ccttcgcccc gtgccccgct
     1801 ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac cgcgttactc ccacaggtga
     1861 gcgggcggga cggcccttct cctccgggct gtaattagcg cttggtttaa tgacggcttg
     1921 tttcttttct gtggctgcgt gaaagccttg aggggctccg ggagggccct ttgtgcgggg
     1981 ggagcggctc ggggggtgcg tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg
     2041 cgctgcccgg cggctgtgag cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg
     2101 tgcgcgaggg gagcgcggcc gggggcggtg ccccgcggtg cggggggggc tgcgagggga
     2161 acaaaggctg cgtgcggggt gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg
     2221 tcgggctgca accccccctg cacccccctc cccgagttgc tgagcacggc ccggcttcgg
     2281 gtgcggggct ccgtacgggg cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc
     2341 aggtgggggt gccgggcggg gcggggccgc ctcgggccgg ggagggctcg ggggaggggc
     2401 gcggcggccc ccggagcgcc ggcggctgtc gaggcgcggc gagccgcagc cattgccttt
     2461 tatggtaatc gtgcgagagg gcgcagggac ttcctttgtc ccaaatctgt gcggagccga
     2521 aatctgggag gcgccgccgc accccctcta gcgggcgcgg ggcgaagcgg tgcggcgccg
     2581 gcaggaagga aatgggcggg gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc
     2641 ctctccagcc tcggggctgt ccgcgggggg acggctgcct tcggggggga cggggcaggg
     2701 cggggttcgg cttctggcgt gtgaccggcg gctctagagc ctctgctaac catgttcatg
     2761 ccttcttctt tttcctacag ctcctgggca acgtgctggt tattgtgctg tctcatcatt
     2821 ttggcaaaga attgatttga taccgcgggc ccgggatccg atatccgcca ccatggtgag
     2881 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     2941 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
     3001 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     3061 caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga
     3121 cttcttcaag tccgccatgc ccgaaggttg gtatcaaaga tctccgaagt tcctattctc
     3181 tagaaagtat aggaacttca taacttcgta tagtacacat tatacgaagt tatataactt
     3241 cgtataggat actttatacg aagttatgga tctccgaagt tcctattctc tagaaagtat
     3301 aggaacttcg gatctataac ttcgtatagc atacattata cgaagttatt caggtggcac
     3361 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     3421 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     3481 tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     3541 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
     3601 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     3661 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     3721 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     3781 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     3841 gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc
     3901 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
     3961 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
     4021 caagaccgac ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg
     4081 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
     4141 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
     4201 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
     4261 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
     4321 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
     4381 actgttcgcc aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg
     4441 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
     4501 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
     4561 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
     4621 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg
     4681 gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc
     4741 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
     4801 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ctagggggag gctaactgaa
     4861 acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat
     4921 aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca gggctggcac
     4981 tctgtcgata ccccaccgag accccattgg ggccaatacg cccgcgtttc ttccttttcc
     5041 ccaccccacc ccccaagttc gggtgaaggc ccagggctcg cagccaacgt cggggcggca
     5101 ggccctgcca tagcctcagg ttactcagga tctggatcta taacttcgta tagcatacat
     5161 tatacgaagt tatggttaca agacaggttt aaggagacca atagaaactg ggcatgtgga
     5221 gacagagaag actcttgggt ttctgatagg cactgactct ctctgcctat tggtctattt
     5281 tcccaccctt aggtgagtaa gggcgaggag gtcatcaaag agttcatgcg cttcaaggtg
     5341 cgcatggagg gctccatgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc
     5401 ccctacgagg gcacccagac cgccaagctg aaggtgacca agggcggccc cctgcccttc
     5461 gcctgggaca tcctgtcccc ccagttcatg tacggctcca aggcgtacgt gaagcacccc
     5521 gccgacatcc ccgattacaa gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg
     5581 atgaacttcg aggacggcgg tctggtgacc gtgacccagg actcctccct gcaggacggc
     5641 acgctgatct acaaggtgaa gatgcgcggc accaacttcc cccccgacgg ccccgtaatg
     5701 cagaagaaga ccatgggctg ggaggcctcc accgagcgcc tgtacccccg cgacggcgtg
     5761 ctgaagggcg agatccacca ggccctgaag ctgaaggacg gcggccacta cctggtggag
     5821 ttcaagacca tctacatggc caagaagccc gtgcaactgc ccggctacta ctacgtggac
     5881 accaagctgg acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgagcgc
     5941 tccgagggcc gccaccacct gttcctgggg catggcaccg gcagcaccgg cagcggcagc
     6001 tccggcaccg cctcctccga ggacaacaac atggccgtca tcaaagagtt catgcgcttc
     6061 aaggtgcgca tggagggctc catgaacggc cacgagttcg agatcgaggg cgagggcgag
     6121 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg cggccccctg
     6181 cccttcgcct gggacatcct gtccccccag ttcatgtacg gctccaaggc gtacgtgaag
     6241 caccccgccg acatccccga ttacaagaag ctgtccttcc ccgagggctt caagtgggag
     6301 cgcgtgatga acttcgagga cggcggtctg gtgaccgtga cccaggactc ctccctgcag
     6361 gacggcacgc tgatctacaa ggtgaagatg cgcggcacca acttcccccc cgacggcccc
     6421 gtaatgcaga agaagaccat gggctgggag gcctccaccg agcgcctgta cccccgcgac
     6481 ggcgtgctga agggcgagat ccaccaggcc ctgaagctga aggacggcgg ccgctacctg
     6541 gtggagttca agaccatcta catggccaag aagcccgtgc aactgcccgg ctactactac
     6601 gtggacacca agctggacat cacctcccac aacgaggact acaccatcgt ggaacagtac
     6661 gagcgctccg agggccgcca ccacctgttc ctgtacggca tggacgagct gtacaagaga
     6721 tccttagagc agaaactcat tagcgaggag gacctgaaca gcgaacagaa actcatttcc
     6781 gaagaggatc tcaactccga gcagaagctg atcagcgagg aggacctgag atcctgagaa
     6841 ttctagataa ctgatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
     6901 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
     6961 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     7021 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     7081 tttaaattgg gcgcgccggc tagaagatgg gcgggagtct tctgggcagg cttaaaggct
     7141 aacctggtgt gtgggcgttg tcctgcaggg gaattgaaca ggtgtaaaat tggagggaca
     7201 agacttccca cagattttcg gttttgtcgg gaagtttttt aataggggca aataaggaaa
     7261 atgggaggat aggtagtcat ctggggtttt atgcagcaaa actacaggtt attattgctt
     7321 gtgatccgcc tcggagtatt ttccatcgag gtagattaaa gacatgctca cccgagtttt
     7381 atactctcct gcttgagatc cttactacag tatgaaatta cagtgtcgcg agttagacta
     7441 tgtaagcaga attttaatca tttttaaaga gcccagtact tcatatccat ttctcccgct
     7501 ccttctgcag ccttatcaaa aggtatttta gaacactcat tttagcccca ttttcattta
     7561 ttatactggc ttatccaacc cctagacaga gcattggcat tttccctttc ctgatcttag
     7621 aagtctgatg actcatgaaa ccagacagat tagttacata caccacaaat cgaggctgta
     7681 gctggggcct caacactgca gttcttttat aactccttag tacacttttt gttgatcctt
     7741 tgccttgatc cttaattttc agtgtctatc acctctcccg tcaggtggtg ttccacattt
     7801 gggcctattc tcagtccagg gagttttaca acaatagatg tattgagaat ccaacctaaa
     7861 gcttaacttt ccactcccat gaatgcctct ctcctttttc tccatttata aactgagcta
     7921 ttaaccatta atggtttcca ggtggatgtc tcctccccca atattacctg atgtatctta
     7981 catattgcca ggctgatatt ttaagacatt aaaaggtata tttcattatt gagccacatg
     8041 gtattgatta ctgcttacta aaattttgtc attgtacaca tctgtaaaag gtggttcctt
     8101 ttggaatgca aagttcaggt gtttgttgtc tttcctgacc taaggtcttg tgagcttgta
     8161 ttttttctat ttaagcagtg ctttctcttg gactggcttg actcatggca ttctacacgt
     8221 tattgctggt ctaaatgtga ttttgccaag cttcttcagg acctataatt ttgcttgact
     8281 tgtagccaaa cacaagtaaa atgattaagc aacaaatgta tttgtgaagc ttggttttta
     8341 ggttgttgtg ttgtgtgtgc ttgtgctcta taataatact atccaggggc tggagaggtg
     8401 gctcggagtt caagagcaca gactgctctt ccagaagtcc tgagttcaat tcccagcaac
     8461 cacatggtgg ctcacaacca tctgtaatgg gatctgatgc cctcttctgg tgtgtctgaa
     8521 gaccacaagt gtattcacat taaataaata aatcctcctt cttcttcttt tttttttttt
     8581 taaagagaat actgtctcca gtagaattta ctgaagtaat gaaatacttt gtgtttgttc
     8641 caatatggta gccaataatc aaattactct ttaagcactg gaaatgttac caaggaacta
     8701 atttttattt gaagtgtaac tgtggacaga ggagccataa ctgcagactt gtgggataca
     8761 gaagaccaat gcagacttta atgtcttttc tcttacacta agcaataaag aaataaaaat
     8821 tgaacttcta gtatcctatt tgtttaaact gctagcttta cttaactttt gtgcttcatc
     8881 tatacaaagc tgaaagctaa gtctgcagcc attactaaac atgaaagcaa gtaatgataa
     8941 ttttggattt caaaaatgta gggccagagt ttagccagcc agtggtggtg cttgccttta
     9001 tgcctttaat cccagcactc tggaggcaga gacaggcaga tctctgagtt tgagcccagc
     9061 ctggtctaca catcaagttc tatctaggat agccaggaat acacacagaa accctgttgg
     9121 ggaggggggc tctgagattt cataaaatta taattgaagc attccctaat gagccactat
     9181 ggatgtggct aaatccgtct acctttctga tgagatttgg gtattatttt ttctgtctct
     9241 gctgttggtt gggtcttttg acactgtggg ctttctttaa agcctccttc ctgccatgtg
     9301 gtctcttgtt tgctactaac ttcccatggc ttaaatggca tggctttttg ccttctaagg
     9361 gcagctgctg agatttgcag cctgatttcc agggtggggt tgggaaatct ttcaaacact
     9421 aaaattgtcc tttaattttt tttttaaaaa atgggttata taataaacct cataaaatag
     9481 ttatgaggag tgaggtggac taatattaaa tgagtccctc ccctataaaa gagctattaa
     9541 ggctttttgt cttatactta actttttttt taaatgtggt atctttagaa ccaagggtct
     9601 tagagtttta gtatacagaa actgttgcat cgcttaatca gattttctag tttcaaatcc
     9661 agagaatcca aattcttcac agccaaagtc aaattaagaa tttctgactt ttaatgttaa
     9721 tttgcttact gtgaatataa aaatgatagc ttttcctgag gcagggtctc actatgtatc
     9781 tctgcctgat ctgcaacaag atatgtagac taaagttctg cctgcttttg tctcctgaat
     9841 actaaggtta aaatgtagta atacttttgg aacttgcagg tcagattctt ttatagggga
     9901 cacactaagg gagcttgggt gatagttggt aaaatgtgtt tcaagtgatg aaaacttgaa
     9961 ttattatcac cgcaacctac tttttaaaaa aaaaagccag gcctgttaga gcatgcttaa
    10021 gggatcccta ggacttgctg agcacacaag agtagttact tggcaggctc ctggtgagag
    10081 catatttcaa aaaacaaggc agacaaccaa gaaactacag ttaaggttac ctgtctttaa
    10141 accatctgca tatacacagg gatattaaaa tattccaaat aatatttcat tcaagttttc
    10201 ccccatcaaa ttgggacatg gatttctccg gtgaataggc agagttggaa actaaacaaa
    10261 tgttggtttt gtgatttgtg aaattgtttt caagtgatag ttaaagccca tgagatacag
    10321 aacaaagctg ctatttcgag gtctcttggt ttatactcag aagcacttct ttgggtttcc
    10381 ctgcactatc ctgatcatgt gctaggccta ccttaggctg attgttgttc aaataaactt
    10441 aagtttcctg tcaggtgatg tcatatgatt tcatatatca aggcaaaaca tgttatatat
    10501 gttaaacatt tgtacttaat gtgaaagtta ggtctttgtg ggtttgattt ttaattttca
    10561 aaacctgagc taaataagtc atttttacat gtcttacatt tggtggaatt gtataattgt
    10621 ggtttgcagg caagactctc tgacctagta accctaccta tagagcactt tgctgggtca
    10681 caagtctagg agtcaagcat ttcaccttga agttgagacg ttttgttagt gtatactagt
    10741 ttatatgttg gaggacatgt ttatccagaa gatattcagg actatttttg actgggctaa
    10801 ggaattgatt ctgattagca ctgttagtga gcattgagtg gcctttaggc ttgaattgga
    10861 gtcacttgta tatctcaaat aatgctggcc ttttttaaaa agcccttgtt ctttatcacc
    10921 ctgttttcta cataattttt gttcaaagaa atacttgttt ggatctcctt ttgacaacaa
    10981 tagcatgttt tcaagccata ttttttttcc tttttttttt tttttttggt ttttcgagac
    11041 agggtttctc tgtatagccc tggctgtcct ggaactcact ttgtagacca ggctggcctc
    11101 gaactcagaa atccgcctgc ctctgcctcc tgagtgccgg gattaaaggc gtgcaccacc
    11161 acgcctggct aagttggata ttttgttata taactataac caatactaac tccactgggt
    11221 ggatttttaa ttcagtcagt agtcttaagt ggtctttatt ggcccttcat taaaatctac
    11281 tgttcactct aacagaggct gttggtacta gtggcactta agcaacttcc tacggatata
    11341 ctagcagatt aagggtcagg gatagaaact agtctagcgt tttgtatacc taccagcttt
    11401 atactacctt gttctgatag aaatatttca ggacatctag cttatcgata ccgtcgacgg
    11461 tatcgataag cttgatatcg aattctaccg ggtaggggag gcgcttttcc caaggcagtc
    11521 tggagcatgc gctttagcag ccccgctggg cacttggcgc tacacaagtg gcctctggcc
    11581 tcgcacacat tccacatcca ccggtaggcg ccaaccggct ccgttctttg gtggcccctt
    11641 cgcgccacct tctactcctc ccctagtcag gaagttcccc cccgccccgc agctcgcgtc
    11701 gtgcaggacg tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga tggacagcac
    11761 cgctgagcaa tggaagcggg taggcctttg gggcagcggc caatagcagc tttgctcctt
    11821 cgctttctgg gctcagaggc tgggaagggg tgggtccggg ggcgggctca ggggcgggct
    11881 caggggcggg gcgggcgccc gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa
    11941 gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg ggcctttcga cctgcaggtc
    12001 ctcgccatgg atcctgatga tgttgttgat tcttctaaat cttttgtgat ggaaaacttt
    12061 tcttcgtacc acgggactaa acctggttat gtagattcca ttcaaaaagg tatacaaaag
    12121 ccaaaatctg gtacacaagg aaattatgac gatgattgga aagggtttta tagtaccgac
    12181 aataaatacg acgctgcggg atactctgta gataatgaaa acccgctctc tggaaaagct
    12241 ggaggcgtgg tcaaagtgac gtatccagga ctgacgaagg ttctcgcact aaaagtggat
    12301 aatgccgaaa ctattaagaa agagttaggt ttaagtctca ctgaaccgtt gatggagcaa
    12361 gtcggaacgg aagagtttat caaaaggttc ggtgatggtg cttcgcgtgt agtgctcagc
    12421 cttcccttcg ctgaggggag ttctagcgtt gaatatatta ataactggga acaggcgaaa
    12481 gcgttaagcg tagaacttga gattaatttt gaaacccgtg gaaaacgtgg ccaagatgcg
    12541 atgtatgagt atatggctca agcctgtgca ggaaatcgtg tcaggcgatc tctttgtgaa
    12601 ggaaccttac ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc
    12661 taaggtaaat ataaaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg
    12721 tgtattttag attccaacct atggaactga tgaatgggag cagtggtgga atgcagatcc
    12781 tagagctcgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
    12841 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
    12901 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
    12961 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
    13021 ctctatggct tctgaggcgg aaagaaccag ctggggctcg agggggggcc cggtacccag
    13081 cttttgttcc ctttagtgag ggttaattgc gcgcttggcg taatcatggt catagctgtt
    13141 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
    13201 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
    13261 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
    13321 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
    13381 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
    13441 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
    13501 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
    13561 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
    13621 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
    13681 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
    13741 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
    13801 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
    13861 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
    13921 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
    13981 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
    14041 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
    14101 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
    14161 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
    14221 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
    14281 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
    14341 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
    14401 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
    14461 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
    14521 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
    14581 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
    14641 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
    14701 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
    14761 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
    14821 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
    14881 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
    14941 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
    15001 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
    15061 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
    15121 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
    15181 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
    15241 aataggggtt ccgcgcacat ttccccgaaa agtgccacct aaattgtaag cgttaatatt
    15301 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
    15361 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
    15421 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
    15481 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
    15541 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
    15601 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
    15661 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
    15721 ccgctacagg gcgcgtcagg tgcgccattc aggctgcgca actgttggga agggcgatcg
    15781 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta
    15841 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgagcgc
    15901 gcgtaatacg actcactata gggcgaattg gagctcca
//
