LOCUS       sequence-210946-        2553 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Control for NMD sensor: pCMV-3XFLAG-Fluc-betaglobin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Baird TD, Cheng KC, Chen YC, Buehler E, Martin SE, Inglese J, Hogg 
            JR
  TITLE     ICE1 promotes the link between splicing and nonsense-mediated mRNA 
            decay.
  JOURNAL   eLife. 2018 Mar 12;7. pii: 33178. doi: 10.7554/eLife.33178.
  PUBMED    29528287
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2018-03-12"; volume: "7. pii: 33178. doi"; pages: " 
            10.7554/eLife.33178"
COMMENT     Sequence Label: pKC-4.04
FEATURES             Location/Qualifiers
     source          1..2553
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             35..100
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             119..1765
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="EDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAH
                     IEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPA
                     NDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSM
                     YTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHAR
                     DPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKI
                     QSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYG
                     LTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSG
                     YVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILL
                     QHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVF
                     VDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(182..200)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     protein_bind    1793..1817
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gagctcgttt agtgaaccgt cagaattaac catggactac aaagaccatg acggtgatta
       61 taaagatcat gacatcgatt acaaggatga cgatgacaag cttgcggccg cgaattcaga
      121 agacgccaaa aacataaaga aaggcccggc gccattctat ccgctggaag atggaaccgc
      181 tggagagcaa ctgcataagg ctatgaagag atacgccctg gttcctggaa caattgcttt
      241 tacagatgca catatcgagg tggacatcac ttacgctgag tacttcgaaa tgtccgttcg
      301 gttggcagaa gctatgaaac gatatgggct gaatacaaat cacagaatcg tcgtatgcag
      361 tgaaaactct cttcaattct ttatgccggt gttgggcgcg ttatttatcg gagttgcagt
      421 tgcgcccgcg aacgacattt ataatgaacg tgaattgctc aacagtatgg gcatttcgca
      481 gcctaccgtg gtgttcgttt ccaaaaaggg gttgcaaaaa attttgaacg tgcaaaaaaa
      541 gctcccaatc atccaaaaaa ttattatcat ggattctaaa acggattacc agggatttca
      601 gtcgatgtac acgttcgtca catctcatct acctcccggt tttaatgaat acgattttgt
      661 gccagagtcc ttcgataggg acaagacaat tgcactgatc atgaactcct ctggatctac
      721 tggtctgcct aaaggtgtcg ctctgcctca tagaactgcc tgcgtgagat tctcgcatgc
      781 cagagatcct atttttggca atcaaatcat tccggatact gcgattttaa gtgttgttcc
      841 attccatcac ggttttggaa tgtttactac actcggatat ttgatatgtg gatttcgagt
      901 cgtcttaatg tatagatttg aagaagagct gtttctgagg agccttcagg attacaagat
      961 tcaaagtgcg ctgctggtgc caaccctatt ctccttcttc gccaaaagca ctctgattga
     1021 caaatacgat ttatctaatt tacacgaaat tgcttctggt ggcgctcccc tctctaagga
     1081 agtcggggaa gcggttgcca agaggttcca tctgccaggt atcaggcaag gatatgggct
     1141 cactgagact acatcagcta ttctgattac acccgagggg gatgataaac cgggcgcggt
     1201 cggtaaagtt gttccatttt ttgaagcgaa ggttgtggat ctggataccg ggaaaacgct
     1261 gggcgttaat caaagaggcg aactgtgtgt gagaggtcct atgattatgt ccggttatgt
     1321 aaacaatccg gaagcgacca acgccttgat tgacaaggat ggatggctac attctggaga
     1381 catagcttac tgggacgaag acgaacactt cttcatcgtt gaccgcctga agtctctgat
     1441 taagtacaaa ggctatcagg tggctcccgc tgaattggaa tccatcttgc tccaacaccc
     1501 caacatcttc gacgcaggtg tcgcaggtct tcccgacgat gacgccggtg aacttcccgc
     1561 cgccgttgtt gttttggagc acggaaagac gatgacggaa aaagagatcg tggattacgt
     1621 cgccagtcaa gtaacaaccg cgaaaaagtt gcgcggagga gttgtgtttg tggacgaagt
     1681 accgaaaggt cttaccggaa aactcgacgc aagaaaaatc agagagatcc tcataaaggc
     1741 caagaagggc ggaaagatcg ccgtggcgaa ttcatcgata gatctgatat caacaagttt
     1801 gtacaaaaaa gcaggctccg cggccgcccc cttcaccgtg catctgactc ctgaggagaa
     1861 gtctgccgtt actgccctgt ggggcaaggt gaacgtggat gaagttggtg gtgaggccct
     1921 gggcaggttg gtatcaaggt tacaagacag gtttaaggag accaatagaa actgggcatg
     1981 tggagacaga gaagactctt gggtttctga taggcactga ctctctctgc ctattggtct
     2041 attttcccac ccttaggctg ctggtggtct acccttggac ccagaggttc tttgagtcct
     2101 ttggggatct gtccactcct gatgctgtta tgggcaaccc taaggtgaag gctcatggca
     2161 agaaagtgct cggtgccttt agtgatggcc tggctcacct ggacaacctc aagggcacct
     2221 ttgccacact gagtgagctg cactgtgaca agctgcacgt ggatcctgag aacttcaggg
     2281 tgagtctgat gggcacctcc tgggtttcct tcccctggct attctgctca accttcctat
     2341 cagaaaaaaa ggggaagcga ttctagggag cagtctccat gactgtgtgt ggagtgttga
     2401 caagagttcg gatattttat tctctactca gaattgctgc tccccctcac tctgttctgt
     2461 gttgtcattt cctctttctt tggtaagctt ttaatttcca gttgcatttt actaaattaa
     2521 ttaagctggg tatttacttc ccatcctgat atc
//
