LOCUS       sequence-211093-         528 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  empty sgRNA cloning vector with MS2-AID3f.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Liu LD, Huang M, Dai P, Liu T, Fan S, Cheng X, Zhao Y, Yeap LS, Meng
            FL
  TITLE     Intrinsic Nucleotide Preference of Diversifying Base Editors Guides 
            Antibody Ex Vivo Affinity Maturation.
  JOURNAL   Cell Rep. 2018 Oct 23;25(4):884-892.e3. doi: 
            10.1016/j.celrep.2018.09.090.
  PUBMED    30355495
REFERENCE   2  (bases 1 to 528)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2018.09"; journalName: "Cell Rep"; date: 
            "2018-10-23- 23"; volume: "25"; issue: "4"; pages: "884-892.e3"
COMMENT     Sequence Label: pLL3 sgRNA(MS2)-MS2-AID3f
FEATURES             Location/Qualifiers
     source          1..528
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggaccccg ctaccttcac ataccaattc aaaaatgtcc gctgggctaa gggtcggcgt
       61 gagacctacc tgtgctacgt agtgaagagg cgtgacagtg ctacatcctt ttcactggac
      121 tttggttatc ttcgcaataa gaacggctgc cacgtggaat tgctcttcct ccgctacatc
      181 tcggactggg acctagaccc tggccgctgc taccgcgtca cctggttcac ctcctggagc
      241 ccctgctacg actgtgcccg acatgtggcc gactttctgc gagggaaccc caacctcagt
      301 ctgaggatct tcaccgcgcg cctctactac ttctgggata ctgactacca agaggggctg
      361 cggcggctgc accgcgccgg ggtgcaaata gccatcatga ccttcaaaga ttatttttac
      421 tgctggaata cttttgtaga aaaccatgaa agaactttca aagcctggga agggctgcat
      481 gaaaattcag ttcgtctctc cagacagctt cggcgcatcc ttcagtaa
//
