LOCUS       sequence-211148-       10438 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Cas9 fusion to HE (HDR-enhancer) domain of human CtIP 
            protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10438)
  AUTHORS   Charpentier M, Khedher AHY, Menoret S, Brion A, Lamribet K, 
            Dardillac E, Boix C, Perrouault L, Tesson L, Geny S, De Cian A, 
            Itier JM, Anegon I, Lopez B, Giovannangeli C, Concordet JP
  TITLE     CtIP fusion to Cas9 enhances transgene integration by 
            homology-dependent repair.
  JOURNAL   Nat Commun. 2018 Mar 19;9(1):1133. doi: 10.1038/s41467-018-03475-7.
  PUBMED    29556040
REFERENCE   2  (bases 1 to 10438)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-03-19"; volume: "9(1):1133. doi"; pages: " 
            10.1038/s41467-018-03475-7"
COMMENT     Sequence Label: pCas9-HE
FEATURES             Location/Qualifiers
     source          1..10438
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        68..447
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        448..651
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     602..622
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     648..672
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      760..769
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             766..4869
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             766..4869
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             766..4869
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             4882..4902
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(5842..5861)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5886..5934
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6136..6564
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6223..6242)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6433..6454
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6573..6593)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6578..6907
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6758..6893
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6820..6839
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6974..7768
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7028..7047)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7638..7657
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7944..8065
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7981..8000)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8035..8054
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8114..8130)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8114..8130)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8127..8149)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8138..8154
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8162..8168,8169..8186,8187..8192))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8207..8228
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8345..8362)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8516..9104)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8596..8615)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9275..10066,10067..10135))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9898..9917
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10136..10240)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10315..10334)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat
       61 acgcgttgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
      121 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
      181 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
      241 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
      301 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
      361 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
      421 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
      481 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
      541 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
      601 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg
      661 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg
      721 gaccgatcca gcctccggac tctagaggat cgaacccttg ccaccatgga caagaagtac
      781 tccattgggc tcgatatcgg cacaaacagc gtcggctggg ccgtcattac ggacgagtac
      841 aaggtgccga gcaaaaaatt caaagttctg ggcaataccg atcgccacag cataaagaag
      901 aacctcattg gcgccctcct gttcgactcc ggggagacgg ccgaagccac gcggctcaaa
      961 agaacagcac ggcgcagata tacccgcaga aagaatcgga tctgctacct gcaggagatc
     1021 tttagtaatg agatggctaa ggtggatgac tctttcttcc ataggctgga ggagtccttt
     1081 ttggtggagg aggataaaaa gcacgagcgc cacccaatct ttggcaatat cgtggacgag
     1141 gtggcgtacc atgaaaagta cccaaccata tatcatctga ggaagaagct tgtagacagt
     1201 actgataagg ctgacttgcg gttgatctat ctcgcgctgg cgcatatgat caaatttcgg
     1261 ggacacttcc tcatcgaggg ggacctgaac ccagacaaca gcgatgtcga caaactcttt
     1321 atccaactgg ttcagactta caatcagctt ttcgaagaga acccgatcaa cgcatccgga
     1381 gttgacgcca aagcaatcct gagcgctagg ctgtccaaat cccggcggct cgaaaacctc
     1441 atcgcacagc tccctgggga gaagaagaac ggcctgtttg gtaatcttat cgccctgtca
     1501 ctcgggctga cccccaactt taaatctaac ttcgacctgg ccgaagatgc caagcttcaa
     1561 ctgagcaaag acacctacga tgatgatctc gacaatctgc tggcccagat cggcgaccag
     1621 tacgcagacc tttttttggc ggcaaagaac ctgtcagacg ccattctgct gagtgatatt
     1681 ctgcgagtga acacggagat caccaaagct ccgctgagcg ctagtatgat caagcgctat
     1741 gatgagcacc accaagactt gactttgctg aaggcccttg tcagacagca actgcctgag
     1801 aagtacaagg aaattttctt cgatcagtct aaaaatggct acgccggata cattgacggc
     1861 ggagcaagcc aggaggaatt ttacaaattt attaagccca tcttggaaaa aatggacggc
     1921 accgaggagc tgctggtaaa gcttaacaga gaagatctgt tgcgcaaaca gcgcactttc
     1981 gacaatggaa gcatccccca ccagattcac ctgggcgaac tgcacgctat cctcaggcgg
     2041 caagaggatt tctacccctt tttgaaagat aacagggaaa agattgagaa aatcctcaca
     2101 tttcggatac cctactatgt aggccccctc gcccggggaa attccagatt cgcgtggatg
     2161 actcgcaaat cagaagagac catcactccc tggaacttcg aggaagtcgt ggataagggg
     2221 gcctctgccc agtccttcat cgaaaggatg actaactttg ataaaaatct gcctaacgaa
     2281 aaggtgcttc ctaaacactc tctgctgtac gagtacttca cagtttataa cgagctcacc
     2341 aaggtcaaat acgtcacaga agggatgaga aagccagcat tcctgtctgg agagcagaag
     2401 aaagctatcg tggacctcct cttcaagacg aaccggaaag ttaccgtgaa acagctcaaa
     2461 gaagactatt tcaaaaagat tgaatgtttc gactctgttg aaatcagcgg agtggaggat
     2521 cgcttcaacg catccctggg aacgtatcac gatctcctga aaatcattaa agacaaggac
     2581 ttcctggaca atgaggagaa cgaggacatt cttgaggaca ttgtcctcac ccttacgttg
     2641 tttgaagata gggagatgat tgaagaacgc ttgaaaactt acgctcatct cttcgacgac
     2701 aaagtcatga aacagctcaa gaggcgccga tatacaggat gggggcggct gtcaagaaaa
     2761 ctgatcaatg ggatccgaga caagcagagt ggaaagacaa tcctggattt tcttaagtcc
     2821 gatggatttg ccaaccggaa cttcatgcag ttgatccatg atgactctct cacctttaag
     2881 gaggacatcc agaaagcaca agtttctggc cagggggaca gtcttcacga gcacatcgct
     2941 aatcttgcag gtagcccagc tatcaaaaag ggaatactgc agaccgttaa ggtcgtggat
     3001 gaactcgtca aagtaatggg aaggcataag cccgagaata tcgttatcga gatggcccga
     3061 gagaaccaaa ctacccagaa gggacagaag aacagtaggg aaaggatgaa gaggattgaa
     3121 gagggtataa aagaactggg gtcccaaatc cttaaggaac acccagttga aaacacccag
     3181 cttcagaatg agaagctcta cctgtactac ctgcagaacg gcagggacat gtacgtggat
     3241 caggaactgg acatcaatcg gctctccgac tacgacgtgg atcatatcgt gccccagtct
     3301 tttctcaaag atgattctat tgataataaa gtgttgacaa gatccgataa aaatagaggg
     3361 aagagtgata acgtcccctc agaagaagtt gtcaagaaaa tgaaaaatta ttggcggcag
     3421 ctgctgaacg ccaaactgat cacacaacgg aagttcgata atctgactaa ggctgaacga
     3481 ggtggcctgt ctgagttgga taaagccggc ttcatcaaaa ggcagcttgt tgagacacgc
     3541 cagatcacca agcacgtggc ccaaattctc gattcacgca tgaacaccaa gtacgatgaa
     3601 aatgacaaac tgattcgaga ggtgaaagtt attactctga agtctaagct ggtctcagat
     3661 ttcagaaagg actttcagtt ttataaggtg agagagatca acaattacca ccatgcgcat
     3721 gatgcctacc tgaatgcagt ggtaggcact gcacttatca aaaaatatcc caagcttgaa
     3781 tctgaatttg tttacggaga ctataaagtg tacgatgtta ggaaaatgat cgcaaagtct
     3841 gagcaggaaa taggcaaggc caccgctaag tacttctttt acagcaatat tatgaatttt
     3901 ttcaagaccg agattacact ggccaatgga gagattcgga agcgaccact tatcgaaaca
     3961 aacggagaaa caggagaaat cgtgtgggac aagggtaggg atttcgcgac agtccggaag
     4021 gtcctgtcca tgccgcaggt gaacatcgtt aaaaagaccg aagtacagac cggaggcttc
     4081 tccaaggaaa gtatcctccc gaaaaggaac agcgacaagc tgatcgcacg caaaaaagat
     4141 tgggacccca agaaatacgg cggattcgat tctcctacag tcgcttacag tgtactggtt
     4201 gtggccaaag tggagaaagg gaagtctaaa aaactcaaaa gcgtcaagga actgctgggc
     4261 atcacaatca tggagcgatc aagcttcgaa aaaaacccca tcgactttct cgaggcgaaa
     4321 ggatataaag aggtcaaaaa agacctcatc attaagcttc ccaagtactc tctctttgag
     4381 cttgaaaacg gccggaaacg aatgctcgct agtgcgggcg agctgcagaa aggtaacgag
     4441 ctggcactgc cctctaaata cgttaatttc ttgtatctgg ccagccacta tgaaaagctc
     4501 aaagggtctc ccgaagataa tgagcagaag cagctgttcg tggaacaaca caaacactac
     4561 cttgatgaga tcatcgagca aataagcgaa ttctccaaaa gagtgatcct cgccgacgct
     4621 aacctcgata aggtgctttc tgcttacaat aagcacaggg ataagcccat cagggagcag
     4681 gcagaaaaca ttatccactt gtttactctg accaacttgg gcgcgcctgc agccttcaag
     4741 tacttcgaca ccaccataga cagaaagcgg tacacctcta caaaggaggt cctggacgcc
     4801 acactgattc atcagtcaat tacggggctc tatgaaacaa gaatcgacct ctctcagctc
     4861 ggtggagaca gcagggctga ccccaagaaa aagaggaagg tgggatccgc tagcatgaac
     4921 atctcgggaa gcagctgtgg aagccctaac tctgcagata catctagtga ctttaaagat
     4981 ctgtggacca aactaaaaga atgtcatgat agagaagtac aaggtttaca agtaaaagta
     5041 accaagctta agcaagagcg aatcttagat gcacaaagac tagaagaatt cttcaccaaa
     5101 aatcaacagc tgagggaaca gcagaaagtc cttcatgaag ccattaaagt tttagaagat
     5161 cggttaagag caggcttatg tgatcgctgt gcagtaactg aagaacatat gcggaaaaaa
     5221 cagcaagagt ttgaaaatat ccggcagcag aatcttaaac ttattacaga acttatgaat
     5281 gaaaggaata ctctacagga agaaaataaa aagctttctg aacaactcca gcagaaaatt
     5341 gagaatgatc aacagcatca agcagctgag cttgaatgtg aggaagacgt tattccagat
     5401 tcaccgataa cagccttctc attttctggc gttaaccggc tacgaagaaa ggagaacccc
     5461 catgtccgat acatagaaca aacacatact aaattggagc actctgtgtg tgcaaatgaa
     5521 atgagaaaag tttccaagtc ttcaactcat ccacaacata atcctaatga aaatgaaatt
     5581 ctagtagctg acacttatga ccaaagtcaa tctccaatgg ccaaagcaca tggaacaagc
     5641 agctataccc ctgataagtc atcttttaat ttagctacag ttgttgctga aacacttgga
     5701 cttggtgttc aagaagaatc tgaaactcaa ggtcccatga gcccccttgg tgatgagctc
     5761 taccactgtc tggaaggaaa tcacaagaaa cagccttttg agtagaccgg ttagtaatga
     5821 gtttaaacgg gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc
     5881 tatgacggca ataaaaagac agaataaaac gcacgggtgt tgggtcgttt gttcataaac
     5941 gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc cattggggcc
     6001 aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt gaaggcccag
     6061 ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc agatctgcgc agctggggct
     6121 ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     6181 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     6241 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     6301 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     6361 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     6421 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     6481 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     6541 tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa
     6601 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     6661 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     6721 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     6781 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     6841 cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     6901 ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcaag agacaggatg
     6961 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
     7021 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
     7081 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
     7141 cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
     7201 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
     7261 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
     7321 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
     7381 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
     7441 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
     7501 gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
     7561 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
     7621 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
     7681 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
     7741 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgcgaa atgaccgacc
     7801 aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt
     7861 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
     7921 tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa
     7981 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     8041 tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct
     8101 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     8161 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     8221 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     8281 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     8341 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     8401 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     8461 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     8521 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     8581 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     8641 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     8701 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     8761 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     8821 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     8881 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     8941 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     9001 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     9061 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     9121 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     9181 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     9241 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     9301 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     9361 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     9421 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     9481 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     9541 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     9601 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     9661 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     9721 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     9781 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     9841 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     9901 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     9961 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
    10021 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
    10081 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
    10141 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
    10201 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
    10261 cacctgacgt cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat
    10321 ctgctctgat gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc
    10381 tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgc
//
