LOCUS       sequence-211167-        8644 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CMV expression vector for BE3-FNLS construct (not codon optimized).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8644)
  AUTHORS   Zafra MP, Schatoff EM, Katti A, Foronda M, Breinig M, Schweitzer AY,
            Simon A, Han T, Goswami S, Montgomery E, Thibado J, Kastenhuber ER, 
            Sanchez-Rivera FJ, Shi J, Vakoc CR, Lowe SW, Tschaharganeh DF, Dow 
            LE
  TITLE     Optimized base editors enable efficient editing in cells, organoids 
            and mice.
  JOURNAL   Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.
  PUBMED    29969439
REFERENCE   2  (bases 1 to 8644)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194."
COMMENT     Sequence Label: pCMV-BE3-FNLS
FEATURES             Location/Qualifiers
     source          1..8644
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        154..357
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     308..328
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     391..410
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        391..409
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      417..426
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             426..491
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             498..518
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             543..1229
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG
                     GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL
                     SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN
                     EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI
                     LWATGLK"
     CDS             join(1278..4676,4677..4679,4680..5276,5277,5278..5280,
                     5281..5282,5283..5285,5286..5378)
                     /codon_start=1
                     /product="Streptococcus pyogenes Cas9 endonuclease with the
                     D1135V/R1335Q/T1337R mutations"
                     /label=Cas9 VQR
                     /note="has an altered recognition specificity for the 
                     protospacer adjacent motif (PAM) (Kleinstiver et al., 
                     2015)"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(1278..1301,1302..1304,1305..5378)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5391..5639
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5652..5672
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5681..5698
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5721..5738)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5727..5951
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6022..6038)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6022..6038)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6035..6057)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6046..6062
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6070..6076,6077..6094,6095..6100))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6115..6136
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6253..6270)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6424..7012)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6504..6523)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7183..7974,7975..8043))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7806..7825
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8044..8148)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8227..8246)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(8418..8644,1..153)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
       61 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      121 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
      181 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
      241 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
      301 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctggt
      361 ttagtgaacc gtcagatccg ctagagatcc taatacgact cactataggg agagccgcca
      421 ccatggacta taaggaccac gacggagact acaaggatca tgatattgat tacaaagacg
      481 atgacgataa gatggcccca aagaagaagc ggaaggtcgg tatccacgga gtcccagcag
      541 ccatgagctc agagactggc ccagtggctg tggaccccac attgagacgg cggatcgagc
      601 cccatgagtt tgaggtattc ttcgatccga gagagctccg caaggagacc tgcctgcttt
      661 acgaaattaa ttgggggggc cggcactcca tttggcgaca tacatcacag aacactaaca
      721 agcacgtcga agtcaacttc atcgagaagt tcacgacaga aagatatttc tgtccgaaca
      781 caaggtgcag cattacctgg tttctcagct ggagcccatg cggcgaatgt agtagggcca
      841 tcactgaatt cctgtcaagg tatccccacg tcactctgtt tatttacatc gcaaggctgt
      901 accaccacgc tgacccccgc aatcgacaag gcctgcggga tttgatctct tcaggtgtga
      961 ctatccaaat tatgactgag caggagtcag gatactgctg gagaaacttt gtgaattata
     1021 gcccgagtaa tgaagcccac tggcctaggt atccccatct gtgggtacga ctgtacgttc
     1081 ttgaactgta ctgcatcata ctgggcctgc ctccttgtct caacattctg agaaggaagc
     1141 agccacagct gacattcttt accatcgctc ttcagtcttg tcattaccag cgactgcccc
     1201 cacacattct ctgggccacc gggttgaaaa gcggcagcga gactcccggg acctcagagt
     1261 ccgccacacc cgaaagtgat aaaaagtatt ctattggttt agccatcggc actaattccg
     1321 ttggatgggc tgtcataacc gatgaataca aagtaccttc aaagaaattt aaggtgttgg
     1381 ggaacacaga ccgtcattcg attaaaaaga atcttatcgg tgccctccta ttcgatagtg
     1441 gcgaaacggc agaggcgact cgcctgaaac gaaccgctcg gagaaggtat acacgtcgca
     1501 agaaccgaat atgttactta caagaaattt ttagcaatga gatggccaaa gttgacgatt
     1561 ctttctttca ccgtttggaa gagtccttcc ttgtcgaaga ggacaagaaa catgaacggc
     1621 accccatctt tggaaacata gtagatgagg tggcatatca tgaaaagtac ccaacgattt
     1681 atcacctcag aaaaaagcta gttgactcaa ctgataaagc ggacctgagg ttaatctact
     1741 tggctcttgc ccatatgata aagttccgtg ggcactttct cattgagggt gatctaaatc
     1801 cggacaactc ggatgtcgac aaactgttca tccagttagt acaaacctat aatcagttgt
     1861 ttgaagagaa ccctataaat gcaagtggcg tggatgcgaa ggctattctt agcgcccgcc
     1921 tctctaaatc ccgacggcta gaaaacctga tcgcacaatt acccggagag aagaaaaatg
     1981 ggttgttcgg taaccttata gcgctctcac taggcctgac accaaatttt aagtcgaact
     2041 tcgacttagc tgaagatgcc aaattgcagc ttagtaagga cacgtacgat gacgatctcg
     2101 acaatctact ggcacaaatt ggagatcagt atgcggactt atttttggct gccaaaaacc
     2161 ttagcgatgc aatcctccta tctgacatac tgagagttaa tactgagatt accaaggcgc
     2221 cgttatccgc ttcaatgatc aaaaggtacg atgaacatca ccaagacttg acacttctca
     2281 aggccctagt ccgtcagcaa ctgcctgaga aatataagga aatattcttt gatcagtcga
     2341 aaaacgggta cgcaggttat attgacggcg gagcgagtca agaggaattc tacaagttta
     2401 tcaaacccat attagagaag atggatggga cggaagagtt gcttgtaaaa ctcaatcgcg
     2461 aagatctact gcgaaagcag cggactttcg acaacggtag cattccacat caaatccact
     2521 taggcgaatt gcatgctata cttagaaggc aggaggattt ttatccgttc ctcaaagaca
     2581 atcgtgaaaa gattgagaaa atcctaacct ttcgcatacc ttactatgtg ggacccctgg
     2641 cccgagggaa ctctcggttc gcatggatga caagaaagtc cgaagaaacg attactccat
     2701 ggaattttga ggaagttgtc gataaaggtg cgtcagctca atcgttcatc gagaggatga
     2761 ccaactttga caagaattta ccgaacgaaa aagtattgcc taagcacagt ttactttacg
     2821 agtatttcac agtgtacaat gaactcacga aagttaagta tgtcactgag ggcatgcgta
     2881 aacccgcctt tctaagcgga gaacagaaga aagcaatagt agatctgtta ttcaagacca
     2941 accgcaaagt gacagttaag caattgaaag aggactactt taagaaaatt gaatgcttcg
     3001 attctgtcga gatctccggg gtagaagatc gatttaatgc gtcacttggt acgtatcatg
     3061 acctcctaaa gataattaaa gataaggact tcctggataa cgaagagaat gaagatatct
     3121 tagaagatat agtgttgact cttaccctct ttgaagatcg ggaaatgatt gaggaaagac
     3181 taaaaacata cgctcacctg ttcgacgata aggttatgaa acagttaaag aggcgtcgct
     3241 atacgggctg gggacgattg tcgcggaaac ttatcaacgg gataagagac aagcaaagtg
     3301 gtaaaactat tctcgatttt ctaaagagcg acggcttcgc caataggaac tttatgcagc
     3361 tgatccatga tgactcttta accttcaaag aggatataca aaaggcacag gtttccggac
     3421 aaggggactc attgcacgaa catattgcga atcttgctgg ttcgccagcc atcaaaaagg
     3481 gcatactcca gacagtcaaa gtagtggatg agctagttaa ggtcatggga cgtcacaaac
     3541 cggaaaacat tgtaatcgag atggcacgcg aaaatcaaac gactcagaag gggcaaaaaa
     3601 acagtcgaga gcggatgaag agaatagaag agggtattaa agaactgggc agccagatct
     3661 taaaggagca tcctgtggaa aatacccaat tgcagaacga gaaactttac ctctattacc
     3721 tacaaaatgg aagggacatg tatgttgatc aggaactgga cataaaccgt ttatctgatt
     3781 acgacgtcga tcacattgta ccccaatcct ttttgaagga cgattcaatc gacaataaag
     3841 tgcttacacg ctcggataag aaccgaggga aaagtgacaa tgttccaagc gaggaagtcg
     3901 taaagaaaat gaagaactat tggcggcagc tcctaaatgc gaaactgata acgcaaagaa
     3961 agttcgataa cttaactaaa gctgagaggg gtggcttgtc tgaacttgac aaggccggat
     4021 ttattaaacg tcagctcgtg gaaacccgcc aaatcacaaa gcatgttgca cagatactag
     4081 attcccgaat gaatacgaaa tacgacgaga acgataagct gattcgggaa gtcaaagtaa
     4141 tcactttaaa gtcaaaattg gtgtcggact tcagaaagga ttttcaattc tataaagtta
     4201 gggagataaa taactaccac catgcgcacg acgcttatct taatgccgtc gtagggaccg
     4261 cactcattaa gaaatacccg aagctagaaa gtgagtttgt gtatggtgat tacaaagttt
     4321 atgacgtccg taagatgatc gcgaaaagcg aacaggagat aggcaaggct acagccaaat
     4381 acttctttta ttctaacatt atgaatttct ttaagacgga aatcactctg gcaaacggag
     4441 agatacgcaa acgaccttta attgaaacca atggggagac aggtgaaatc gtatgggata
     4501 agggccggga cttcgcgacg gtgagaaaag ttttgtccat gccccaagtc aacatagtaa
     4561 agaaaactga ggtgcagacc ggagggtttt caaaggaatc gattcttcca aaaaggaata
     4621 gtgataagct catcgctcgt aaaaaggact gggacccgaa aaagtacggt ggcttcgata
     4681 gccctacagt tgcctattct gtcctagtag tggcaaaagt tgagaaggga aaatccaaga
     4741 aactgaagtc agtcaaagaa ttattgggga taacgattat ggagcgctcg tcttttgaaa
     4801 agaaccccat cgacttcctt gaggcgaaag gttacaagga agtaaaaaag gatctcataa
     4861 ttaaactacc aaagtatagt ctgtttgagt tagaaaatgg ccgaaaacgg atgttggcta
     4921 gcgccggaga gcttcaaaag gggaacgaac tcgcactacc gtctaaatac gtgaatttcc
     4981 tgtatttagc gtcccattac gagaagttga aaggttcacc tgaagataac gaacagaagc
     5041 aactttttgt tgagcagcac aaacattatc tcgacgaaat catagagcaa atttcggaat
     5101 tcagtaagag agtcatccta gctgatgcca atctggacaa agtattaagc gcatacaaca
     5161 agcacaggga taaacccata cgtgagcagg cggaaaatat tatccatttg tttactctta
     5221 ccaacctcgg cgctccagcc gcattcaagt attttgacac aacgatagat cgcaaacgat
     5281 acacttctac caaggaggtg ctagacgcga cactgattca ccaatccatc acgggattat
     5341 atgaaactcg gatagatttg tcacagcttg ggggtgactc tggtggttct actaatctgt
     5401 cagatattat tgaaaaggag accggtaagc aactggttat ccaggaatcc atcctcatgc
     5461 tcccagagga ggtggaagaa gtcattggga acaagccgga aagcgatata ctcgtgcaca
     5521 ccgcctacga cgagagcacc gacgagaatg tcatgcttct gactagcgac gcccctgaat
     5581 acaagccttg ggctctggtc atacaggata gcaacggtga gaacaagatt aagatgctct
     5641 ctggtggttc tcccaagaag aagaggaaag tctaaccggt catcatcacc atcaccattg
     5701 agtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
     5761 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     5821 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     5881 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
     5941 gggctctatg gcttctgagg cggaaagaac cagctggggc tcgataccgt cgacctctag
     6001 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     6061 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctagggtg cctaatgagt
     6121 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     6181 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     6241 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     6301 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     6361 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     6421 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     6481 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     6541 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     6601 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     6661 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     6721 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     6781 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     6841 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     6901 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     6961 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     7021 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     7081 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     7141 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     7201 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     7261 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     7321 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     7381 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     7441 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     7501 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     7561 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     7621 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     7681 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     7741 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     7801 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     7861 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     7921 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     7981 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     8041 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     8101 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     8161 aaaagtgcca cctgacgtcg acggatcggg agatcgatct cccgatcccc tagggtcgac
     8221 tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt
     8281 gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac
     8341 cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg
     8401 ggccagatat acgcgttgac attgattatt gactagttat taatagtaat caattacggg
     8461 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
     8521 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     8581 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     8641 ccac
//
