LOCUS       sequence-211196-        1530 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A genetically encoded voltage indicator (Aahn), capable of optically
            monitoring both plasma membrane and internal membrane potentials..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Sepehri Rad M, Cohen LB, Braubach O, Baker BJ
  TITLE     Monitoring voltage fluctuations of intracellular membranes.
  JOURNAL   Sci Rep. 2018 May 2;8(1):6911. doi: 10.1038/s41598-018-25083-7.
  PUBMED    29720664
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2018-05-2"; volume: "8(1):6911. doi"; pages: " 
            10.1038/s41598-018-25083-7"
COMMENT     Sequence Label: Aahn
FEATURES             Location/Qualifiers
     source          1..1530
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             776..1492
                     /codon_start=1
                     /product="pH-sensitive mutant of green fluorescent protein 
                     (Ng et al., 2002)"
                     /label=superecliptic pHluorin
                     /note="excited strongly by blue light at neutral pH but 
                     minimally fluorescent at acidic pH"
                     /translation="MSKGEELFTGAVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNDHQVYIMADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLFTTSTLSKDPNEKRDHMVLLE
                     FVTADGITHGMDELYK"
     primer_bind     1429..1449
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 aatacgactc actataggga gacccaagct ggctagcacc atggagggat tcgacggttc
       61 agattttagt cctccagctg atttagttgg cgttgacggt gcagtcatgc ggaacgtcgt
      121 tgacgtcacg ataaatggtg acgtcactgc tccgccgaaa gctgcgccaa gaaaaagtga
      181 atcggtaaag aaagttcatt ggaatgatgt agaccaagga ccgagtgaaa aaccagagac
      241 aagacaggag gaacgaatag atatacccga gatttcaggt ctatggtggg gcgagaatga
      301 acatggagtg gacgatggga gaatggagat acctactact ggtgtaggtc gcgtccagtt
      361 tcgtgtccga gcagtgattg atcatctagg gatgcgagtc tttggagtct tcctaatttt
      421 cttggacatc atcctcatga tcattgatct cagtctttcc gataaaagtg aatcttcaca
      481 atccttttat gacgggatgg ctttggctct ttcttgttat ttcatgctgg atttaggatt
      541 aaggatattt gcctacgggc ccaagaattt cttcaccaac ccctgggagg ttgctgatgg
      601 tttgattatc gttgtcacat tcgtcgtcac gatattttac actgtgttag atgaatacgt
      661 gcaagaaaca ggagccgatg gtttggggca attggttgtg ttggcccgtt tgctgcgtgt
      721 ggttcgatta gcaagaatat tttattccca ccaacaaatg aaggctgggg atcccatgag
      781 taaaggagaa gaacttttca ctggagctgt cccaattctt gttgaattag atggtgatgt
      841 taatgggcac aaattttctg tcagtggaga gggtgaaggt gatgcaacat acggaaaact
      901 tacccttaaa tttatttgca ctactggaaa actacctgtt ccatggccaa cacttgtcac
      961 tactttaact tatggtgttc aatgcttttc aagataccca gatcatatga aacggcatga
     1021 ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa agaactatat ttttcaaaga
     1081 tgacgggaac tacaagacac gtgctgaagt caagtttgaa ggtgataccc ttgttaatag
     1141 aatcgagtta aaaggtattg attttaaaga agatggaaac attcttggac acaaattgga
     1201 atacaactat aacgatcacc aggtgtacat catggcagac aaacaaaaga atggaatcaa
     1261 agctaacttc aaaattagac acaacattga agatggaggc gttcaactag cagaccatta
     1321 tcaacaaaat actccaattg gcgatgggcc cgtcctttta ccagacaacc attacctgtt
     1381 tacaacttct actctttcga aagatcccaa cgaaaagaga gaccacatgg tccttcttga
     1441 gtttgtaaca gctgatggga ttacacatgg catggatgaa ctatacaaat gatgactcga
     1501 ggtctagagg gcccgtttaa acccgctgat
//
