LOCUS       sequence-211254-        7485 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length BMR1_02g04275 
            ectodomain with no N-linked glycans upon cotransfection with BirA in
            mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7485)
  AUTHORS   Elton CM, Rodriguez M, Ben Mamoun C, Lobo CA, Wright GJ
  TITLE     A library of recombinant Babesia microti cell surface and secreted 
            proteins for diagnostics discovery and reverse vaccinology.
  JOURNAL   Int J Parasitol. 2019 Feb;49(2):115-125. doi: 
            10.1016/j.ijpara.2018.10.003. Epub 2018 Oct 24.
  PUBMED    30367868
REFERENCE   2  (bases 1 to 7485)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ijpara.2018.10.003"; journalName: "Int J Parasitol"; 
            date: "2019-02"; volume: "49"; issue: "2"; pages: "115-125"
COMMENT     Sequence Label: BMR1_02g04275-bio-His
FEATURES             Location/Qualifiers
     source          1..7485
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2649..2666
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2718..2773
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2772..2791)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5009..5027)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5095..5199
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5200..5268,5269..6060)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5418..5437)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6231..6819
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6720..6739
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6973..6990
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7107..7128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7143..7148,7149..7166,7167..7173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7181..7197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7186..7208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7205..7221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7205..7221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgccg
     1201 gcaacggcga cgtgaaccag tacagcagcg acttcggcag agccctgaac gacctgatga
     1261 tcgccttcaa cgaggccaag aagatgtacg ccaagttcag cgagcagatc acctacacca
     1321 tgttccacac ctacaagaac agcatcgaca tcctgaaggc cgacgagaag aacggcggcc
     1381 acaagaacta cctggaaaag aaagagatcg agctgaagtc caagaccgtc gagttcgacg
     1441 tgatcttcag caacatcgac ctgaacaacg ccgccgtgaa gaacgagatc atcaagctgc
     1501 tgaacgacat cagcaccatc tccaccgaca tcaagagcat cgtggacgag atctactaca
     1561 aggccctggg caccatcgag ggcgagaacg ccgagaactt cgagtacgag atcaagaaga
     1621 agaaggccga gctgctgcgg aacctgctga atgacaacat caagcccatc atgggctacc
     1681 tgaccgagat ctacaacatg cacatcccca tcatcagcaa caaggccgag ttcaacgata
     1741 tcaagaaggc cttcgagaag cacgagctgg aagccaacgt gctgatctcc aagatcctgg
     1801 aaaacaacca gaacttcggc accaacttca acgacatcct caacgaagtg aacggcgcca
     1861 tcgaggaatt caacaaggcc atcgacgtga tgaacaatgc catcggcgac ctgggcatcg
     1921 tgatcgacag cggcatcatc agctccatca agagccacat cagcacaatc gccaagatca
     1981 gcaaggccat catccctggc gcgccgtcga cctccatcac ggcctataag agtgaggggg
     2041 agtcagcgga gttctccttc ccactcaacc ttggagagga aagcctgcag ggagagttga
     2101 gatggaaggc agagaaggct ccttcttccc agtcctggat caccttctcc ctaaagaacc
     2161 aaaaggtgtc tgtgcagaag tctactagca accccaagtt ccagctgtcc gaaacgctcc
     2221 cactcaccct tcagataccc caggtctccc ttcagtttgc tggttctggc aacctgaccc
     2281 tgactctgga cagagggata ctgtatcagg aagtgaacct ggtggtgatg aaagtgactc
     2341 agcccgacag caacactttg acctgtgagg tgatgggacc cacctcaccc aagatgagac
     2401 tgatcttgaa gcaggagaat caggaggcca gggtctccag gcaggagaaa gtgattcaag
     2461 tgcaggcccc tgaagcaggg gtgtggcaat gtctactgag tgaaggtgaa gaggtcaaga
     2521 tggactccaa gatccaggtt ttatccaaag ggttgaattc cggcagcctg caccacatcc
     2581 tggacgccca gaagatgctg tggaaccaca gggacaggaa cctgcctcct ctggcccctc
     2641 tgggccccca ccaccatcac caccattgag tctagacacg tgattaatta aggatccccc
     2701 gacctcgacc tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt
     2761 tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttggtcg agatccctcg
     2821 gagatctcta gctagaggat cgatccccgc cccggacgaa ctaaacctga ctacgacatc
     2881 tctgcccctt cttcgcgggg cagtgcatgt aatcccttca gttggttggt acaacttgcc
     2941 aactgggccc tgttccacat gtgacacggg gggggaccaa acacaaaggg gttctctgac
     3001 tgtagttgac atccttataa atggatgtgc acatttgcca acactgagtg gctttcatcc
     3061 tggagcagac tttgcagtct gtggactgca acacaacatt gcctttatgt gtaactcttg
     3121 gctgaagctc ttacaccaat gctgggggac atgtacctcc caggggccca ggaagactac
     3181 gggaggctac accaacgtca atcagagggg cctgtgtagc taccgataag cggaccctca
     3241 agagggcatt agcaatagtg tttataaggc ccccttgtta accctaaacg ggtagcatat
     3301 gcttcccggg tagtagtata tactatccag actaacccta attcaatagc atatgttacc
     3361 caacgggaag catatgctat cgaattaggg ttagtaaaag ggtcctaagg aacagcgata
     3421 tctcccaccc catgagctgt cacggtttta tttacatggg gtcaggattc cacgagggta
     3481 gtgaaccatt ttagtcacaa gggcagtggc tgaagatcaa ggagcgggca gtgaactctc
     3541 ctgaatcttc gcctgcttct tcattctcct tcgtttagct aatagaataa ctgctgagtt
     3601 gtgaacagta aggtgtatgt gaggtgctcg aaaacaaggt ttcaggtgac gcccccagaa
     3661 taaaatttgg acggggggtt cagtggtggc attgtgctat gacaccaata taaccctcac
     3721 aaaccccttg ggcaataaat actagtgtag gaatgaaaca ttctgaatat ctttaacaat
     3781 agaaatccat ggggtgggga caagccgtaa agactggatg tccatctcac acgaatttat
     3841 ggctatgggc aacacataat cctagtgcaa tatgatactg gggttattaa gatgtgtccc
     3901 aggcagggac caagacaggt gaaccatgtt gttacactct atttgtaaca aggggaaaga
     3961 gagtggacgc cgacagcagc ggactccact ggttgtctct aacacccccg aaaattaaac
     4021 ggggctccac gccaatgggg cccataaaca aagacaagtg gccactcttt tttttgaaat
     4081 tgtggagtgg gggcacgcgt cagcccccac acgccgccct gcggttttgg actgtaaaat
     4141 aagggtgtaa taacttggct gattgtaacc ccgctaacca ctgcggtcaa accacttgcc
     4201 cacaaaacca ctaatggcac cccggggaat acctgcataa gtaggtgggc gggccaagat
     4261 aggggcgcga ttgctgcgat ctggaggaca aattacacac acttgcgcct gagcgccaag
     4321 cacagggttg ttggtcctca tattcacgag gtcgctgaga gcacggtggg ctaatgttgc
     4381 catgggtagc atatactacc caaatatctg gatagcatat gctatcctaa tctatatctg
     4441 ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg
     4501 ggtagtatat gctatcctaa tttatatctg ggtagcatag gctatcctaa tctatatctg
     4561 ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa tctgtatccg
     4621 ggtagcatat gctatcctaa tagagattag ggtagtatat gctatcctaa tttatatctg
     4681 ggtagcatat actacccaaa tatctggata gcatatgcta tcctaatcta tatctgggta
     4741 gcatatgcta tcctaatcta tatctgggta gcataggcta tcctaatcta tatctgggta
     4801 gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaattta tatctgggta
     4861 gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     4921 gtatatgcta tcctaatctg tatccgggta gcatatgcta tcctcatgat aagctgtcaa
     4981 acatgagaat tttcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     5041 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     5101 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     5161 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     5221 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     5281 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     5341 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     5401 agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag
     5461 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     5521 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     5581 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     5641 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     5701 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg
     5761 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     5821 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     5881 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     5941 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     6001 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     6061 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     6121 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     6181 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     6241 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     6301 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     6361 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     6421 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     6481 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     6541 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     6601 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     6661 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     6721 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     6781 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     6841 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     6901 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     6961 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     7021 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     7081 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     7141 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     7201 cacacaggaa acagctatga ccatgattac gccaagctct agctagaggt cgaccaattc
     7261 tcatgtttga cagcttatca tcgcagatcc gggcaacgtt gttgccattg ctgcaggcgc
     7321 agaactggta ggtatggaag atctatacat tgaatcaata ttggcaatta gccatattag
     7381 tcattggtta tatagcataa atcaatattg gctattggcc attgcatacg ttgtatctat
     7441 atcataatat gtacatttat attggctcat gtccaatatg accgc
//
