LOCUS       sequence-211328-       11066 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11066)
  AUTHORS   Ahmad K, Henikoff S
  TITLE     Centromeres are specialized replication domains in heterochromatin.
  JOURNAL   J Cell Biol 2001 Apr 2;153(1):101-10.
  PUBMED    11285277
REFERENCE   2  (bases 1 to 11066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol"; date: "2001-04-2"; volume: "153(1)"; pages: "101-10"
COMMENT     Sequence Label: pPgCdH3
FEATURES             Location/Qualifiers
     source          1..11066
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(235..823)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(315..334)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(994..1785,1786..1854))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1617..1636
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1855..1959)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2027..2045
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2083..2105)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2205..2224
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    join(2762..2792,2793..2994)
                     /label=P element 3' end
                     /note="P element 3' end"
     protein_bind    3056..3150
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        3169..3407
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     promoter        3632..3870
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     CDS             4749..5465
                     /codon_start=1
                     /gene="mgfp5"
                     /product="GFP with folding enhancement mutations"
                     /label=mgfp5
                     /note="suitable for expression in plants due to removal of 
                     a cryptic intron"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFNDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKA
                     NFKTRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(4779..4807)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     5402..5422
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     intron          5555..5620
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5750..5770
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    6199..6333
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6236..6255)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6290..6309
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6360..6378)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(6830..6901,7303..7576,7651..8305,8367..8682,8886..9017,
                     9088..9702)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    join(10481..11035,11036..11066)
                     /label=P element 5' end
ORIGIN
        1 ggccagaccc acgtagtcca gcggcagatc ggcggcggag aagttaagcg tctccaggat
       61 gaccttgccc gaactggggc acgtggtgtt cgacgatgtg cagctaattt cgcccggctc
      121 cacgtccgcc cattggttaa tcagcagacc ctcgttggcg taacggaacc atgagaggta
      181 cgacaaccat ttgaggtata ctggcaccga gcccgagttc aagaagaagg cgtttttcca
      241 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      301 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
      361 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
      421 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
      481 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
      541 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
      601 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
      661 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
      721 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
      781 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
      841 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
      901 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
      961 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     1021 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     1081 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     1141 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     1201 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     1261 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     1321 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     1381 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     1441 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     1501 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     1561 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     1621 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     1681 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     1741 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     1801 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     1861 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     1921 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     1981 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     2041 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     2101 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     2161 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
     2221 tgtactgaga gtgcaccata tgcggtgtga aataccgcac cgaatcgcgc ggaactaacg
     2281 acagtcgctc caaggtcgtc gaacaaaagg tgaatgtgtt gcggagagcg ggtgggagac
     2341 agcgaaagag caactacgaa acgtggtgtg gtggaggtga attatgaaga gggcgcgcga
     2401 tttgaaaagt atgtatataa aaaatatatc ccggtgtttt atgtagcgat aaacgagttt
     2461 ttgatgtaag gtatgcaggt gtgtaagtct tttggttaga agacaaatcc aaagtctact
     2521 tgtggggatg ttcgaagggg aaatacttgt attctatagg tcatatcttg tttttattgg
     2581 cacaaatata attacattag ctttttgagg gggcaataaa cagtaaacac gatggtaata
     2641 atggtaaaaa aaaaaaacaa gcagttattt cggatatatg tcggctactc cttgcgtcgg
     2701 gcccgaagtc ttagagccag atatgcgagc acccggaagc tcacgatgag aatggccaga
     2761 ccatgatgaa ataacataag gtggtcccgt cggcaagaga catccactta acgtatgctt
     2821 gcaataagtg cgagtgaaag gaatagtatt ctgagtgtcg tattgagtct gagtgagaca
     2881 gcgatatgat tgttgattaa cccttagcat gtccgtgggg tttgaattaa ctcataatat
     2941 taattagacg aaattatttt taaagtttta tttttaataa tttgcgagta cgcaaagctt
     3001 ggctgcatcc aacgcgttgg gagctctccg gatccaagct tgcatgcctg caggtcggag
     3061 tactgtcctc cgagcggagt actgtcctcc gagcggagta ctgtcctccg agcggagtac
     3121 tgtcctccga gcggagtact gtcctccgag cggagactct agcgagcgcc ggagtataaa
     3181 tagaggcgct tcgtctacgg agcgacaatt caattcaaac aagcaaagtg aacacgtcgc
     3241 taagcgaaag ctaagcaaat aaacaagcgc agctgaacaa gctaaacaat ctgcagtaaa
     3301 gtgcaagtta aagtgaatca attaaaagta accagcaacc aagtaaatca actgcaacta
     3361 ctgaaatctg ccaagaagta attattgaat acaagaagag aactctgaat agggaattgg
     3421 gaattccaaa acaaactggt tattgtggta ggtcatttgt ttggcagaaa gaaaactcga
     3481 gaaatttctc tggccgttat tcgttattct ctcttttctt tttgggtctc tccctctctg
     3541 cactaatgct ctctcactct gtcacacagt aaacggcata ctgctctcgt tggttcgaga
     3601 gagcgcgcct cgaatgttcg cgaaaagagc gccggagtat aaatagaggc gcttcgtcta
     3661 cggagcgaca attcaattca aacaagcaaa gtgaacacgt cgctaagcga aagctaagca
     3721 aataaacaag cgcagctgaa caagctaaac aatctgcagt aaagtgcaag ttaaagtgaa
     3781 tcaattaaaa gtaaccagca accaagtaaa tcaactgcaa ctactgaaat ctgccaagaa
     3841 gtaattattg aatacaagaa gagaactctg ggggatctga tcaagagaca ggatgaggtc
     3901 tagacgacat ggctgtatct tcagtgctct gctaatgagt tttaatggcg aaatagggta
     3961 attatccaaa aatttaggag gtaaacaatt tcacatcagc tgtgcactaa cgatgggaat
     4021 tgatcgatac ctgtatagta gtaattaatc gatttctagg ggtataaatg atcactgcaa
     4081 aataagttaa agtactggac gtctttaaac aattaccatg tattcccatt aatattaatt
     4141 gtcacataaa taaacatatt ttaattctca actgctagaa atatatttag ttagtcagtg
     4201 cccattcaaa tcggtatcgg tactgggcat tatttgcttt gctttatacg gtcacactgt
     4261 tccaaaaaag cgggaattct ttaaaaatat tttaagcaaa taccgaaaat ttactagagg
     4321 agatggctcg taccaagcaa actgctcgca aatcgactgg tggaaaggcg ccacgcaaac
     4381 aactggctac taaggccgct cgcaagagtg ctccagccac cggaggtgtg aagaagcccc
     4441 accgctatcg ccctggaacc gtggccttgc gtgaaattcg tcgctaccaa aagagcaccg
     4501 agcttctaat ccgcaagctg cctttccagc gtctggtgcg tgaaatcgct caggacttta
     4561 agacggactt gcgattccag agctcggcgg ttatggctct gcaggaagct agcgaagcct
     4621 acctggttgg tctcttcgaa gataccaact tgtgtgccat tcatgccaag cgtgtcacca
     4681 taatgcccaa agacatccag ttagcgcgac gcattcgcgg cgagcgtgct cagcggccgg
     4741 tcgcaacaat gagtaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat
     4801 tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa
     4861 catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct gttccatggc
     4921 caacacttgt cactactttc acttatggtg ttcaatgctt ttcaagatac ccagatcata
     4981 tgaagcggca cgacttcttc aagagcgcca tgcctgaggg atacgtgcag gagaggacca
     5041 tcttcttcaa tgacgacggg aactacaaga cacgtgctga agtcaagttt gagggagaca
     5101 ccctcgtcaa caggatcgag cttaagggaa tcgatttcaa ggaggacgga aacatcctcg
     5161 gccacaagtt ggaatacaac tacaactccc acaacgtata catcatggcc gacaagcaaa
     5221 agaacggcat caaagccaac ttcaagaccc gccacaacat cgaagacggc ggcgtgcaac
     5281 tcgctgatca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt ttaccagaca
     5341 accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag agagaccaca
     5401 tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat gaactataca
     5461 aataaagatc cactagagga tctttgtgaa ggaaccttac ttctgtggtg tgacataatt
     5521 ggacaaacta cctacagaga tttaaagctc taaggtaaat ataaaatttt taagtgtata
     5581 atgtgttaaa ctactgattc taattgtttg tgtattttag attccaacct atggaactga
     5641 tgaatgggag cagtggtgga atgcctttaa tgaggaaaac ctgttttgct cagaagaaat
     5701 gccatctagt gatgatgagg ctactgctga ctctcaacat tctactcctc caaaaaagaa
     5761 gagaaaggta gaagacccca aggactttcc ttcagaattg ctaagttttt tgagtcatgc
     5821 tgtgtttagt aatagaactc ttgcttgctt tgctatttac accacaaagg aaaaagctgc
     5881 actgctatac aagaaaatta tggaaaaata tttgatgtat agtgccttga ctagagatca
     5941 taatcagcca taccacattt gtagaggttt tacttgcttt awatagtaat agaactcttg
     6001 cttgctttgc tatttacacc acaaaggaaa aagctgcact gctatacaag aaaattatgg
     6061 aaaaatattt gatgtatagt gccttgacta gagatcataa tcagccatac cacatttgta
     6121 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
     6181 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     6241 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     6301 aaactcatca atgtatctta tcatgtctgg atccactaga aggcctaatt ctagtatgta
     6361 tgtaagttaa taaaacccat ttttgcggaa agtagataaa aaaaacattt ttttttttta
     6421 ctgcactgga tatcattgaa cttatctgat cagttttaaa tttacttcga tccaagggta
     6481 tttgatgtac caggttcttt cgattacctc tcactcaaaa tgacattcca ctcaaagtca
     6541 gcgctgtttg cctccttctc tgtccacaga aatatcgccg tctctttcgc cgctgcgtcc
     6601 gctatctctt tcgccaccgt ttgtagcgtt acgtagcgtc aatgtccgcc ttcagttgca
     6661 ttttgtcagc ggtttcgtga cgaagctcca agcggtttac gccatcaatt aaacacaaag
     6721 tgctgtgcca aaactcctct cgcttcttat ttttgtttgt tttttgagtg attggggtgg
     6781 tgattggttt tgggtgggta agcaggggaa agtgtgaaaa atcccggcaa tgggccaaga
     6841 ggatcaggag ctattaattc gcggaggcag caaacaccca tctgccgagc atctgaacaa
     6901 tgtgagtagt acatgtgcat acatcttaag ttcacttgat ctataggaac tgcgattgca
     6961 acatcaaatt gtctgcggcg tgagaactgc gacccacaaa aatcccaaac cgcaattgca
     7021 caaacaaata gtgacacgaa acagattatt ctggtagctg ttctcgctat ataagacaat
     7081 ttttgagatc atatcatgat caagacatct aaaggcattc attttcgact atattctttt
     7141 ttacaaaaaa tataacaacc agatatttta agctgatcct agatgcacaa aaaataaata
     7201 aaagtataaa cctacttcgt aggatacttc ggggtacttt ttgttcgggg ttagatgagc
     7261 ataacgcttg tagttgatat ttgagatccc ctatcattgc agggtgacag cggagcggct
     7321 tcgcagagct gcattaacca gggcttcggg caggccaaaa actacggcac gctccggcca
     7381 cccagtccgc cggaggactc cggttcaggg agcggccaac tagccgagaa cctcacctat
     7441 gcctggcaca atatggacat ctttggggcg gtcaatcagc cgggctccgg atggcggcag
     7501 ctggtcaacc ggacacgcgg actattctgc aacgagcgac acataccggc gcccaggaaa
     7561 catttgctca agaacggtga gtttctattc gcagtcggct gatctgtgtg aaatcttaat
     7621 aaagggtcca attaccaatt tgaaactcag tttgcggcgt ggcctatccg ggcgaacttt
     7681 tggccgtgat gggcagttcc ggtgccggaa agacgaccct gctgaatgcc cttgcctttc
     7741 gatcgccgca gggcatccaa gtatcgccat ccgggatgcg actgctcaat ggccaacctg
     7801 tggacgccaa ggagatgcag gccaggtgcg cctatgtcca gcaggatgac ctctttatcg
     7861 gctccctaac ggccagggaa cacctgattt tccaagccat ggtgcggatg ccacgacatc
     7921 tgacctatcg gcagcgagtg gcccgcgtgg atcaggtgat ccaggagctt tcgctcagca
     7981 aatgtcagca cacgatcatc ggtgtgcccg gcagggtgaa aggtctgtcc ggcggagaaa
     8041 ggaagcgtct ggcattcgcc tccgaggctc taaccgatcc gccgcttctg atctgcgatg
     8101 agcccacctc cggactggac tcctttaccg cccacagcgt cgtccaggtg ctgaagaagc
     8161 tgtcgcagaa gggcaagacc gtcatcctga ccattcatca gccgtcttcc gagctgtttg
     8221 agctctttga caagatcctt ctgatggccg agggcagggt agctttcttg ggcactccca
     8281 gcgaagccgt cgacttcttt tcctagtgag ttcgatgtgt ttattaaggg tatctagtat
     8341 tacataacat ctcaactcct atccagcgtg ggtgcccagt gtcctaccaa ctacaatccg
     8401 gcggactttt acgtacaggt gttggccgtt gtgcccggac gggagatcga gtcccgtgat
     8461 cggatcgcca agatatgcga caattttgcc attagcaaag tagcccggga tatggagcag
     8521 ttgttggcca ccaaaaatct ggagaagcca ctggagcagc cggagaatgg gtacacctac
     8581 aaggccacct ggttcatgca gttccgggcg gtcctgtggc gatcctggct gtcggtgctc
     8641 aaggaaccac tcctcgtaaa agtgcgactt attcagacaa cggtgagtgg ttccagtgga
     8701 aacaaatgat ataacgctta caattcttgg aaacaaattc gctagatttt agatagaatt
     8761 gcctgattcc acacccttct tagttttttt caatgagatg tatagtttat agttttgcag
     8821 aagataaata aatttcattt aactcgcgaa tattaatgag atgcgagtaa cattttaatt
     8881 tgcagatggt tgccatcttg attggcctca tctttttggg ccaacaactc acgcaagtgg
     8941 gtgtgatgaa tatcaacgga gccatcttcc tcttcctgac caacatgacc tttcaaaacg
     9001 tctttgccac gataaatgta agtcatgttt agaatacatt tgcatttcaa taatttacta
     9061 actttctaat gaatcgattc gatttaggtg ttcacctcag agctgccagt ttttatgagg
     9121 gaggcccgaa gtcgacttta tcgctgtgac acatactttc tgggcaaaac gattgccgaa
     9181 ttgccgcttt ttctcacagt gccactggtc ttcacggcga ttgcctatcc gatgatcgga
     9241 ctgcgggccg gagtgctgca cttcttcaac tgcctggcgc tggtcactct ggtggccaat
     9301 gtgtcaacgt ccttcggata tctaatatcc tgcgccagct cctcgacctc gatggcgctg
     9361 tctgtgggtc cgccggttat cataccattc ctgctctttg gcggcttctt cttgaactcg
     9421 ggctcggtgc cagtatacct caaatggttg tcgtacctct catggttccg ttacgccaac
     9481 gagggtctgc tgattaacca atgggcggac gtggagccgg gcgaaattag ctgcacatcg
     9541 tcgaacacca cgtgccccag ttcgggcaag gtcatcctgg agacgcttaa cttctccgcc
     9601 gccgatctgc cgctggacta cgtgggtctg gccattctca tcgtgagctt ccgggtgctc
     9661 gcatatctgg ctctaagact tcgggcccga cgcaaggagt agccgacata tatccgaaat
     9721 aactgcttgt tttttttttt taccattatt accatcgtgt ttactgttta ttgccccctc
     9781 aaaaagctaa tgtaattata tttgtgccaa taaaaacaag atatgaccta tagaatacaa
     9841 gtatttcccc ttcgaacatc cccacaagta gactttggat ttgtcttcta accaaaagac
     9901 ttacacacct gcatacctta catcaaaaac tcgtttatcg ctacataaaa caccgggata
     9961 tattttttat atacatactt ttcaaatcgc gcgccctctt cataattcac ctccaccaca
    10021 ccacgtttcg tagttgctct ttcgctgtct cccacccgct ctccgcaaca cattcacctt
    10081 ttgttcgacg accttggagc gactgtcgtt agttccgcgc gattcggttc gctcaaatgg
    10141 ttccgagtgg ttcatttcgt ctcaatagaa attagtaata aatatttgta tgtacaattt
    10201 atttgctcca atatatttgt atatatttcc ctcacagcta tatttattct aatttaatat
    10261 tatgactttt taaggtaatt ttttgtgacc tgttcggagt gattagcgtt acaatttgaa
    10321 ctgaaagtga catccagtgt ttgttccttg tgtagatgca tctcaaaaaa atggtgggca
    10381 taatagtgtt gtttatatat atcaaaaata acaactataa taataagaat acatttaatt
    10441 tagaaaatgc ttggatttca ctggaactag aattaattcg gctgctgctc taaacgacgc
    10501 atttcgtact ccaaagtacg aattttttcc ctcaagctct tattttcatt aaacaatgaa
    10561 caggacctaa cgcacagtca cgttattgtt tacataaatg atttttttta ctattcaaac
    10621 ttactctgtt tgtgtactcc cactggtata gccttctttt atcttttctg gttcaggctc
    10681 tatcacttta ctaggtacgg catctgcgtt gagtcgcctc cttttaaatg tctgaccttt
    10741 tgcaggtgca gccttccact gcgaatcatt aaagtgggta tcacaaattt gggagttttc
    10801 accaaggctg cacccaaggc tctgctccca caattttctc ttaatagcac acttcggcac
    10861 gtgaattaat tttactccag tcacagcttt gcagcaaaat ttgcaatatt tcattttttt
    10921 ttattccacg taagggttaa tgttttcaaa aaaaaattcg tccgcacaca acctttcctc
    10981 tcaacaagca aacgtgcact gaatttaagt gtatacttcg gtaagcttcg gctatcgacg
    11041 ggaccacctt atgttatttc atcatg
//
