LOCUS       sequence-211340-       10381 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targeted DNA methylation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10381)
  AUTHORS   Lu Z, Liu Z, Mao W, Wang X, Zheng X, Chen S, Cao B, Huang S, Zhang 
            X, Zhou T, Zhang Y, Huang X, Sun Q, Li JD
  TITLE     Locus-specific DNA methylation of Mecp2 promoter leads to 
            autism-like phenotypes in mice.
  JOURNAL   Cell Death Dis. 2020 Feb 3;11(2):85. doi: 10.1038/s41419-020-2290-x.
  PUBMED    32015323
REFERENCE   2  (bases 1 to 10381)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Death 
            Dis."; date: "2020-02-3"; volume: "11(2):85. doi"; pages: " 
            10.1038/s41419-020-2290-x"
COMMENT     Sequence Label: 
            pSt1374m-EF1alpha-N-NLS-DNMT3L-L-DNMT3Amut-L-dcas9-NLS
FEATURES             Location/Qualifiers
     source          1..10381
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        238..1419
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          469..1411
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1368..1388
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     1469..1488
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1469..1487
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1516..1536
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2191..2208
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             7225..7245
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7276..7332
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7333..8043
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7478..7496)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7746..7765
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(8063..8080)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8069..8293
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8455..8472)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8626..9214)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8706..8725)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9385..10176,10177..10245))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10008..10027
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10246..10350)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtgtgaggc
      241 tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga
      301 ggggtcggca attgaaccgg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat
      361 gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta
      421 gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg
      481 tgtggttccc gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc ttgaattact
      541 tccacgcccc tggctgcagt acgtgattct tgatcccgag cttcgggttg gaagtgggtg
      601 ggagagttcg aggccttgcg cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg
      661 gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc
      721 tttcgataag tctctagcca tttaaaattt ttgatgacct gctgcgacgc tttttttctg
      781 gcaagatagt cttgtaaatg cgggccaaga tctgcacact ggtatttcgg tttttggggc
      841 cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg
      901 agcgcggcca ccgagaatcg gacgggggta gtctcaagct ggccggcctg ctctggtgcc
      961 tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca aggctggccc ggtcggcacc
     1021 agttgcgtga gcggaaagat ggccgcttcc cggccctgct gcagggagct caaaatggag
     1081 gacgcggcgc tcgggagagc gggcgggtga gtcacccaca caaaggaaaa gggcctttcc
     1141 gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca ggcacctcga
     1201 ttagttctcg agcttttgga gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat
     1261 ggagtttccc cacactgagt gggtggagac tgaagttagg ccagcttggc acttgatgta
     1321 attctccttg gaatttgccc tttttgagtt tggatcttgg ttcattctca agcctcagac
     1381 agtggttcaa agtttttttc ttccatttca ggtgtcgtgg agctctctgg ctaactagag
     1441 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
     1501 gctagcacca tgggacctaa gaaaaagagg aaggtggcgg ccgctggcgg cagcatgttc
     1561 gaaaccgtgc ctgtgtggag gagacagcca gtccgggtgc tgtccctttt tgaagacatc
     1621 aagaaagagc tgacgagttt gggctttttg gaaagtggtt ctgacccggg acaactgaag
     1681 catgtggttg atgtcacaga cacagtgagg aaggatgtgg aggagtgggg acccttcgat
     1741 cttgtgtacg gcgccacacc tcccctgggc cacacctgtg accgtcctcc cagctggtac
     1801 ctgttccagt tccaccggct cctgcagtac gcacggccca agccaggcag ccccaggccc
     1861 ttcttctgga tgttcgtgga caatctggtg ctgaacaagg aagacctgga cgtcgcatct
     1921 cgcttcctgg agatggagcc agtcaccatc ccagatgtcc acggcggatc cttgcagaat
     1981 gctgtccgcg tgtggagcaa catcccagcc ataaggagca gcaggcactg ggctctggtt
     2041 tcggaagaag aattgtccct gctggcccag aacaagcaga gctcgaagct cgcggccaag
     2101 tggcccacca agctggtgaa gaactgcttt ctccccctaa gagaatattt caagtatttt
     2161 tcaagcagcg ggcgcagctt cagcagcgga ctagtgcccc gcggcagcca cccagctgag
     2221 aagaggaagc ccatccgggt gctgtctctc tttgatggaa tcgctacagg gctcctggtg
     2281 ctgaaggact tgggcattca ggtggaccgc tacattgcct cggaggtgtg tgaggactcc
     2341 atcacggtgg gcatggtgcg gcaccagggg aagatcatgt acgtcgggga cgtccgcagc
     2401 gtcacacaga agcatatcca ggagtggggc ccattcgatc tggtgattgg gggcagtccc
     2461 tccaatgacc tctccatcgt caaccctgct cgcaagggcc tctacgaggg cactggccgg
     2521 ctcttctttg agttctaccg cctcctgcat gatgcgcggc ccaaggaggg agatgatcgc
     2581 cccttcttct ggctctttga gaatgtggtg gccatgggcg ttagtgacaa gagggacatc
     2641 tcgcgatttc tcgagtccaa ccctgtgatg attgatgcca aagaagtgtc agctgcacac
     2701 agggcccgct acttctgggg taaccttccc ggtatgaaca ggccgttggc atccactgtg
     2761 aatgataagc tggagctgca ggagtgtctg gagcatggca ggatagccaa gttcagcaaa
     2821 gtgaggacca ttactacgag gtcaaactcc ataaagcagg gcaaagacca gcattttcct
     2881 gtcttcatga atgagaaaga ggacatctta tggtgcactg aaatggaaag ggtatttggt
     2941 ttcccagtcc actatactga cgtctccaac atgagccgct tggcgaggca gagactgctg
     3001 ggccggtcat ggagcgtgcc agtcatccgc cacctcttcg ctccgctgaa ggagtatttt
     3061 gcgtgtgtga gcggcagcga gacccccggt accagcgaga gcgccacccc cgagagcgac
     3121 aagaaatact ctattggact ggctatcggg acaaactccg ttggctgggc cgtcataacc
     3181 gacgagtata aggtgccaag caagaaattc aaggtgctgg gtaatactga ccgccattca
     3241 atcaagaaga acctgatcgg agcactcctc ttcgactccg gtgaaaccgc tgaagctact
     3301 cggctgaagc ggaccgcaag gcggagatac acccgccgca agaatcggat atgttatctg
     3361 caagagatct ttagcaacga aatggctaag gtggacgact ccttctttca ccgcctggaa
     3421 gagagctttc tggtggagga ggataagaaa cacgagaggc accctatatt cggaaatatc
     3481 gtggatgagg tggcttacca tgaaaagtat cctacaatct accatctgag gaagaagctg
     3541 gtggacagca ccgataaagc agacctgagg ctcatctatc tggccctggc tcatatgata
     3601 aagtttagag gacactttct gatcgagggc gacctgaatc ccgataattc cgatgtggat
     3661 aaactcttca ttcaactggt gcagacatat aaccaactgt tcgaggagaa tcccataaac
     3721 gcttctggtg tggatgccaa ggctattctg tccgctcggc tgtccaagtc acgcagactg
     3781 gagaatctga ttgcccaact gccaggagaa aagaagaacg gcctgtttgg gaacctcatc
     3841 gccctgagcc tgggcctgac acctaacttc aagtccaatt ttgatctggc cgaagatgct
     3901 aaactccagc tctccaagga cacctatgac gatgatctgg acaacctgct cgcacagata
     3961 ggcgaccagt acgccgatct ctttctggct gctaagaatc tctccgacgc cattctgctg
     4021 agcgacatac tccgggtcaa cactgagatc accaaagcac ctctgagcgc ctccatgata
     4081 aaacgctatg atgaacacca tcaagacctg actctgctca aagccctcgt gaggcaacag
     4141 ctgccagaga agtacaaaga gatattcttc gaccagagca agaatggata tgccggatac
     4201 atcgatggcg gagcatcaca ggaagaattt tacaagttca tcaaaccaat cctcgagaag
     4261 atggacggta ctgaagagct gctggtgaag ctgaacaggg aggacctgct gaggaagcag
     4321 aggacctttg ataatggctc cattccacat cagatacacc tgggagagct gcatgcaatc
     4381 ctccgcaggc aggaggattt ctatcctttc ctgaaggata accgggagaa gatagagaag
     4441 atcctgacct tcaggatccc ttattacgtc ggccctctgg ctagaggcaa ctcccgcttc
     4501 gcttggatga ccaggaaatc tgaggagaca attactcctt ggaacttcga agaggtcgtg
     4561 gataagggcg caagcgccca gtcattcatc gaacggatga ccaatttcga taagaacctg
     4621 ccaaacgaga aggtcctgcc caaacattca ctcctgtacg agtatttcac cgtctataac
     4681 gagctgacta aagtgaagta cgtgaccgag ggcatgagga agcctgcctt cctgtccgga
     4741 gagcagaaga aggctatcgt tgatctgctc ttcaagacta atagaaaggt gacagtgaag
     4801 cagctcaagg aggattactt taagaagatc gaatgctttg actcagtgga aatctctggc
     4861 gtggaggacc gctttaatgc cagcctgggc acttaccatg atctgctgaa gataatcaaa
     4921 gacaaagatt tcctcgataa tgaggagaac gaggacatcc tggaagatat cgtgctgacc
     4981 ctgactctgt tcgaggatag agagatgatc gaagagcgcc tgaagaccta tgcccatctg
     5041 tttgacgata aagtcatgaa acagctcaag cggcggcgct acactgggtg gggtagactc
     5101 tccaggaaac tcataaacgg catccgcgac aaacagagcg gaaagaccat cctggatttc
     5161 ctgaaatccg acggattcgc taacaggaac ttcatgcaac tgattcacga tgactctctg
     5221 acatttaaag aggacatcca gaaggcacag gtgagcggtc aaggcgacag cctgcacgag
     5281 cacatcgcca acctcgctgg atcacccgcc ataaagaagg gaatactgca gacagtcaag
     5341 gtcgtggacg aactcgtcaa agtgatgggt cggcacaagc cagagaatat cgttatcgaa
     5401 atggcaaggg agaaccaaac cacccagaag ggccagaaga actctcggga acggatgaaa
     5461 agaatcgaag agggaattaa ggagctggga tctcagatac tgaaggagca ccctgtggag
     5521 aatacacagc tccagaacga gaaactctac ctgtactacc tccagaacgg gcgggacatg
     5581 tacgttgacc aggaactcga catcaaccgg ctgtccgatt atgacgtgga cgctattgtt
     5641 ccacagtcct tcctcaaaga tgactccatt gacaacaagg tgctgaccag atccgataag
     5701 gcccgcggta agtctgacaa tgttccatca gaagaggtgg tcaagaagat gaagaattac
     5761 tggcggcagc tcctcaacgc caaactgatc acccagcgga agtttgacaa tctgactaag
     5821 gcagaaagag gaggtctgag cgaactcgac aaggccggct ttattaagag gcaactggtc
     5881 gaaacacgcc agattaccaa acacgtggca caaatcctcg actctaggat gaacactaag
     5941 tacgatgaga acgataagct gatcagggaa gtgaaagtga taactctgaa gagcaagctg
     6001 gtgtctgact tccggaagga ctttcaattc tacaaagttc gcgaaataaa caattaccat
     6061 catgctcacg atgcctatct caatgctgtc gttggcaccg ccctgatcaa gaaataccct
     6121 aaactggagt ctgagttcgt gtacggtgac tataaagtct acgatgtgag gaagatgata
     6181 gcaaagtctg agcaagagat tggcaaagcc accgccaagt acttcttcta ctctaatatc
     6241 atgaatttct ttaagactga gataaccctg gctaacggcg aaatccggaa gcgcccactg
     6301 atcgaaacaa acggagaaac aggagaaatc gtgtgggata aaggcaggga cttcgcaact
     6361 gtgcggaagg tgctgtccat gccacaagtc aatatcgtga agaagaccga agtgcagacc
     6421 ggcggattct caaaggagag catcctgcca aagcggaact ctgacaagct gatcgccagg
     6481 aagaaagatt gggacccaaa gaagtatggc ggtttcgatt cccctacagt ggcttattcc
     6541 gttctggtcg tggcaaaagt ggagaaaggc aagtccaaga aactcaagtc tgttaaggag
     6601 ctgctcggaa ttactattat ggagagatcc agcttcgaga agaatccaat cgatttcctg
     6661 gaagctaagg gctataaaga agtgaagaaa gatctcatca tcaaactgcc caagtactct
     6721 ctctttgagc tggagaatgg taggaagcgg atgctggcct ccgccggaga gctgcagaaa
     6781 ggaaacgagc tggctctgcc ctccaaatac gtgaacttcc tgtatctggc ctcccactac
     6841 gagaaactca aaggtagccc tgaagacaat gagcagaagc aactctttgt tgagcaacat
     6901 aaacactacc tggacgaaat cattgaacag attagcgagt tcagcaagcg ggttattctg
     6961 gccgatgcaa acctcgataa agtgctgagc gcatataata agcacaggga caagccaatt
     7021 cgcgaacaag cagagaatat tatccacctc tttactctga ctaatctggg cgctcctgct
     7081 gccttcaagt atttcgatac aactattgac aggaagcggt acacctctac caaagaagtt
     7141 ctcgatgcca ccctgataca ccagtcaatt accggactgt acgagactcg catcgacctg
     7201 tctcagctcg gcggcgacgg ttctcccaag aagaagagga aagtctcgag cggtggagct
     7261 gcaggaggat ccggcgccac gaacttctct ctgttaaagc aagcaggaga cgtggaagaa
     7321 aaccccggtc ctatggtgag caagggcgag gaggataaca tggccatcat caaggagttc
     7381 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
     7441 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
     7501 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
     7561 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
     7621 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
     7681 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
     7741 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
     7801 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     7861 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     7921 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
     7981 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     8041 taagtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     8101 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     8161 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     8221 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     8281 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccat
     8341 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     8401 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     8461 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     8521 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     8581 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     8641 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     8701 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     8761 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     8821 atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     8881 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     8941 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     9001 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     9061 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     9121 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     9181 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     9241 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     9301 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     9361 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     9421 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     9481 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     9541 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     9601 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     9661 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     9721 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     9781 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     9841 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     9901 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     9961 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
    10021 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
    10081 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
    10141 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
    10201 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
    10261 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
    10321 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
    10381 c
//
