LOCUS       sequence-211438-       16444 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16444)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 16444)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHKU32.2-2.2
FEATURES             Location/Qualifiers
     source          1..16444
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1177..1476
                     /label=ADH2 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 2 (ADH2) gene"
     promoter        3854..4074
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             4075..4878
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     CDS             11365..11376
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(12330..12350)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(12330..12348)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(12687..13275)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12767..12786)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13446..14237,14238..14306))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14069..14088
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14307..14411)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      14438..15780
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(15401..15448)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     15821..15839
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(16181..16197)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(16181..16197)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 atttcagatt gagagaatga aaaaaaaaaa aaaaaaaaag gcagaggaga gcatagaaat
       61 ggggttcact ttttggtaaa gctatagcat gcctatcaca tataaataga gtgccagtag
      121 cgactttttt cacactcgaa atactcttac tactgctctc ttgttgtttt tatcacttct
      181 tgtttcttct tggtaaatag aatatcaagc tacaaaaagc atacaatcaa ctatcaacta
      241 ttaactatat cgtaatacac aatgagtggg gagcgtaaag atgttgagtt caaaactctg
      301 gatggcttga ccctgcgcgg ctggttgttt cagggaccca agggcggtcc tgcagtagtt
      361 gtcaatggtg catttaacag cccaaaagag atctttgtag ccagcgtcgc cgaatggttc
      421 gggaagaacg gcgtgacggc tctcgtttac gacgcccgca cgctgggcca gagcgatggc
      481 ctgcctcgaa acgacctcga cccgcaaaag atggcggagg acaaccacga cgctgtcact
      541 ttcctgcaag atgggggatg ggtcgaccca gaccgtatcg ccatctgggg cttcttctat
      601 agctccggca ttgcgctcga ggctgctgct tttgacaagc gcgtcaaggc ggtcatcgca
      661 caaggtctga tgccggactg gtgcctcaac ccgccggacg aggctgccat agttgcgagt
      721 gcggtcgcag atcgggctag ccagcttcgt ggcaacccgc ctcagtacat cccccttctc
      781 aacgacaagg gcgagcacct gatgcacttc aagtatctgg ccgagatgac ccccgagcag
      841 aagcaccatc tccccgcctg ggtccacggg gccaagaaaa acgccccgag cttcaacgac
      901 cacatgacga tccagagctt ctaccgccat gctaagtgga agccaatgaa tctcttcgcg
      961 gccgtcaacc caaccccggt catgattctg acgcctgagg acgacgagat cgtgccgccc
     1021 gcattccaac ggcgcatttt tgatagcctg cagtcgccac agaagaggta tgagattgta
     1081 aagggcagag gtcacatgaa ctttttgaag gatgtaaact ttgatgagct cttgggcggc
     1141 cagctagcgt tcctcaagga ggtgatgaag ttctgagcgg atctcttatg tctttacgat
     1201 ttatagtttt cattatcaag tatgcctata ttagtatata gcatctttag atgacagtgt
     1261 tcgaagtttc acgaataaaa gataatattc tactttttgc tcccaccgcg tttgctagca
     1321 cgagtgaaca ccatccctcg cctgtgagtt gtacccattc ctctaaactg tagacatggt
     1381 agcttcagca gtgttcgtta tgtacggcat cctccaacaa acagtcggtt atagtttgtc
     1441 ctgctcctct gaatcgtctc cctcgatatt tctcattttc cttcgcatgc cagcattgaa
     1501 atgatcgaag ttcaatgatg aaacggtaat tcttctgtca tttactcatc tcatctcatc
     1561 aagttatata attctatacg gatgtaattt ttcacttttc gtcttgacgt ccaccctata
     1621 atttcaatta ttgaaccctc acgatccagt tctccagtga cacagccttt atctggtcaa
     1681 acctttcttt ctaatcacct atgctgatgc ttaattaagg gatttttgtc tccatcaacg
     1741 gcatgcgccc aaaaatgacg ttttttttaa cccatagaca cgaaactacc cattttccac
     1801 cggcctgacc taccaccgga acaacggcca tctccaactt gcaagttggg gaaattaaga
     1861 gcatcgcagg tttaatggaa gaaaaaaaaa aggtacagca cagcgcaaat ggagttagtt
     1921 cccttatgtc acacactcac acacagtcgg tcagatcaag catactgggt gcgtataaat
     1981 agagtggcca ttgccaccct gtttatctca aaatctgtct tgttagtggt cttctccctt
     2041 tttcaggtta caattctctt gtttctactt agtatataag tatatcaagc tatattaagc
     2101 atactatcaa ctgtcaactc tatcctcaaa atacaataca aaatgggcat cgagctgaaa
     2161 caacccgact ctgcgcatct gccgcacaca aacggctcat ctggcacact gcctgagaga
     2221 tcatcaccaa ctggaatcgc tgttttgatt gttggcggag gagtagccgg cttactggcc
     2281 ggtctcgagt gctggcgtca gggccacgag cggtggccag acatggccaa ggagaacgac
     2341 cgcatttcat acgagatgtg gatttcatgg cataaaatca atggggacct gatttccggt
     2401 ccggctcctt tccgtcccaa tgctcaaaag aaagatggcc ctgaaggtcc aaatactctg
     2461 caaaccgcca agatatatag acacagtcgt ccgaccatgc acaagatgct cgccgatcag
     2521 gttgagagag ttggactcac ggtggaatac ggcaaacacg ttactcaata ccaagaggac
     2581 cctgattctt ccaaggctta tgtgattttg gaaaataacg aaaagatcga ggcggacttg
     2641 gttgtcgcag ctgacggaat tggcagcaag tcttatagta tcactttggg ccgtcaagtc
     2701 ccggcccgcc ccacagggtt ttcaatctat cgagcgttgc tgcctatcga tcaggctctt
     2761 gtagatcccg ttatcgatga gaagttccct ctgttagaga atgggatgtc ctccacccaa
     2821 ctctggatgg gcattgagtc ctggtctaac aaggttggac ccgaggtcgt gcttcaaacc
     2881 acggccacga ttgacggttg gcccgacttc gcggatcgat tgatcaaggc aacagccaag
     2941 gatcagatcc acgacttcaa actcatgtgg cgtgagcctc aaccttgctg ggtttcccca
     3001 ggcggccgag ttgtgcagat cggtgatgcg gcgcacagtt ttcttccatc ctccgggaac
     3061 ggtgccacgc agggtatgga agacgccgtc tctctggcag cttgcttgcg acttgctggg
     3121 aaagacaatg tgccctgggc aacccgtgtt cataacaaac tgaggtttga gcgggtatcc
     3181 tgtctgcaaa tgctgggcgt cctcaaccat gagatgcgca atcgctctgc caatgcggat
     3241 gcttcacaga cgaagcccat cggcatactt ggcagctgga tttggaaaca cgatccagag
     3301 cattatgcaa tcgaaaacta cgagaaggcg ttaaggcacc tgacgagtgg gagtgaattt
     3361 ctcaatacta atgtccctcc tggacatgta taccagccgt ggaccattga tgaaatcctg
     3421 aaggccaagg aaacaggcag ggagattgtc ttggatgggg actgggatta aacaaatcgc
     3481 tcttaaatat atacctaaag aacattaaag ctatattata agcaaagata cgtaaatttt
     3541 gcttatatta ttatacacat atcatatttc tatattttta agatttggtt atataatgta
     3601 cgtaatgcaa aggaaataaa ttttatacat tattgaacag cgtccaagta actacattat
     3661 gtgcactaat agtttagcgt cgtgaagact ttattgtgtc gcgaaaagta aaaattttaa
     3721 aaattagagc accttgaact tgcgaaaaag gttctcatca actgtttaaa aggaggatat
     3781 caggtcctat ttctgacaaa caatatacaa atttagtttc aaagatgaat cagtgcgcga
     3841 aggacataac tcattcaatt caattcatca tttttttttt attctttttt ttgatttcgg
     3901 tttccttgaa atttttttga ttcggtaatc tccgaacaga aggaagaacg aaggaaggag
     3961 cacagactta gattggtata tatacgcata tgtagtgttg aagaaacatg aaattgccca
     4021 gtattcttaa cccaactgca cagaacaaaa acctgcagga aacgaagata aatcatgtcg
     4081 aaagctacat ataaggaacg tgctgctact catcctagtc ctgttgctgc caagctattt
     4141 aatatcatgc acgaaaagca aacaaacttg tgtgcttcat tggatgttcg taccaccaag
     4201 gaattactgg agttagttga agcattaggt cccaaaattt gtttactaaa aacacatgtg
     4261 gatatcttga ctgatttttc catggagggc acagttaagc cgctaaaggc attatccgcc
     4321 aagtacaatt ttttactctt cgaagacaga aaatttgctg acattggtaa tacagtcaaa
     4381 ttgcagtact ctgcgggtgt atacagaata gcagaatggg cagacattac gaatgcacac
     4441 ggtgtggtgg gcccaggtat tgttagcggt ttgaagcagg cggcagaaga agtaacaaag
     4501 gaacctagag gccttttgat gttagcagaa ttgtcatgca agggctccct atctactgga
     4561 gaatatacta agggtactgt tgacattgcg aagagcgaca aagattttgt tatcggcttt
     4621 attgctcaaa gagacatggg tggaagagat gaaggttacg attggttgat tatgacaccc
     4681 ggtgtgggtt tagatgacaa gggagacgca ttgggtcaac agtatagaac cgtggatgat
     4741 gtggtctcta caggatctga cattattatt gttggaagag gactatttgc aaagggaagg
     4801 gatgctaagg tagagggtga acgttacaga aaagcaggct gggaagcata tttgagaaga
     4861 tgcggccagc aaaactaaaa aactgtatta taagtaaatg catgtatact aaactcacaa
     4921 attagagctt caatttaatt atatcagtta ttaccctatg cggtgtgaaa taccgcacag
     4981 atgcgtaagg agaaaatacc gcatcaggat aggaaaaaac cgagcttcct ttcatccggc
     5041 gcggctgtgt tctacatatc actgaagctc cgggtatttt aagttataca agggaaagat
     5101 gccggctaga ctagcaagtt ttaggctgct taacattatg gataggcgga taaagggccc
     5161 aaacaggatt gtaaagctta gacgcttctg gttggacaat ggtacgtttg tgtattaagt
     5221 aaggcttggc tggggatagc aacattgggc agagtataga agaccacaaa aaaaaggtat
     5281 ataagggcag agaagtcttt gtaatgtgtg taacttctct tccatgtgta atcagtattt
     5341 ctacttactt cttaaatata cagaagtaag acagataacc aacagccttt cccagatata
     5401 catatatatc tttatttcag cttaaacaat aattatattt gtttaactca aaaataaaaa
     5461 aaaaaaacca aactcacgca actaattatt ccataataaa ataacaacat ggatcgcttt
     5521 cgcagctata ccgagccatg ggaccccatc cgatatggca tctgggccat ctatgcccaa
     5581 tcaaaagagg cctttttcag tggcagatac cccgatcttt ttaacctcaa atatctccta
     5641 gactcggggt tttatttgtg gtttaaagtt ctgaatgagc atttttgccg ctttgagcaa
     5701 aatcaaacca ctatcccagt tcttgtccca gcttctactg gtgtggtact tgagctcggt
     5761 cccggaatgg gtaaccaact gcaccgcttc gacaggacaa aggtgactcg tgtcgtgggc
     5821 gtggagagca actcgcactt cacacgagat attgagacac aggtcaagaa aaacggtctt
     5881 gaagacattt acgagctcgt tacatgccgt gtggaagata gcgacgtgct agaaacgcat
     5941 ggtgttgttg ccgagagtgt ggatactgtc ctgagcatcc aggttctgtg ctcggtggac
     6001 aacccggaag ccgttatcaa ggagctttac cggctgctta agcctggtgg gaagttcata
     6061 ttctgggagc atcaccgtaa ctcggaccgt actactgccg caatccaatt tcatcgcctc
     6121 ccgatagacg tggtcgggcg ctctaagatg gacgacctct tacagctgga gcagtttgct
     6181 ctggcttcca aagtacaagc cgaaattaac aaccacacag gcgtctccga caagaccctt
     6241 gccgaattca tcatcgccca gcggctcgaa tgcgaagacc ttgccgcttt caagcagagg
     6301 ctaaaatcta tcggcgccga tttccctacg agtttggtcg agagtataga ccggctgtgc
     6361 aggtcactac acccgaagct caagaagaag caaggcgccg cgaacaagat cgaagaggaa
     6421 gagcatccga cgcgaagtaa tgaggagaag gcaaaaatat tttcagggtt ggcgcttcct
     6481 gacaaggaag tcccgcctga gtcgaatggc gatgcgatag atgataccct ggcgcagttg
     6541 gagagtttag gagggaggcc gcgcaagccg aaagagtcgc gaaaacgttc acggtccccc
     6601 gaagacggcg acagagattc gaggagacga aaccatagag aacgagatcg gtcacgcgac
     6661 agatcgcgag accgagattc gcggaggaag agatatagat caaggtcgag atcaccaccg
     6721 aggtaccaag gagaactgga ccccgcgccg attatcgaca agatctatga cggaaaggtc
     6781 acgggcgtca aggattttgg cgcatttgtg tccctagaag gtatcaaggg gcgatgtact
     6841 ggcctaatac atatatcgag gctggccgaa ggccgacgca ttggccaccc ttctgaggta
     6901 gtatctatgg gtgagcccgt caaggtcaaa gtcgtcagca tcgaaggaaa caggataggc
     6961 ctgtcgctca aggatgtcga ccaacacacc ggtgaggatc tcgcggcccc ggctcccgcc
     7021 tttggatccg gtgccaacat ggaggccttg ggaagccgac atggcttcgt cgaccagccc
     7081 acatcattcc gcaatccaca gccgccgagg cacaagaagc aattgacaga ttcggacaga
     7141 tgggagatac gacagctcat ccgctccgga gtagcgaagg catcggactt tcccgaactt
     7201 gaggaggaga cgaaagcagt ccataaggaa gacggtggca tggagctgga ggaggatgtc
     7261 gatattgaag ttcgagagga ggaaccccca ttcttggttg gccaaacaaa gcagtctctg
     7321 gaactttccc cgatccgcgt tgtcaaggct cctgatggtt cgatgaaccg agcggccgta
     7381 acgggatcgg cgctcgtgag ggacaggaag gaagctcgcc agcaagaggc agatgcggaa
     7441 gcgcatgcag cgcccaagga tgacttatcc gcgcagtggc atgaccctat ggcagacccg
     7501 gataagcgaa catttgcgag cgagctccga aaggcccgga taaacgcaaa gtcggatgtt
     7561 gtgccggagt ggaagaagac cgtccagaat agggaccagt ctttcggaaa gcgaaccaac
     7621 cttagcatca aggaacagcg agagggcctc cccatattcg cctttaggtc acagctcatc
     7681 agtgcggtga aggagaacca gattcttatc gttgttggtg agacgggatc cggcaaaacc
     7741 acgcagctca cccagtatct tgctgaagca ggctttgcaa atagtggcat gattgggtgt
     7801 acacagccgc gtcgtgtcgc ggccatgtct gtcgccaagc gtgtctccga ggaagttggc
     7861 tgtaagcttg gcgaggaggt cggatacacg gttcgtttcg acgacactac cactccttcg
     7921 accaggatca agtacatgac ggatggtatg ttacaaagag agatcctcat ggatggcgat
     7981 ctgaagcgct actccgtcat catgttggac gaggcccacg agcgaaccat cgccacagat
     8041 gttcttttcg cccttttgaa gaagacggtc aagcgtcggc cggacctcaa gattatcgtc
     8101 acctctgcga ccctcgacgc agataaattc tcgagctatt ttaacgaatg ccctatcttt
     8161 acgattcctg gacgaacgta ccccgtggaa atcttatatt cgagagagcc cgagagcgac
     8221 tacctcgagg ctgcccttct tactgttatg cagattcact tgacagaacc caaaggagat
     8281 attcttctct tcttaacggg ccaggaggag attgacacct catgcgaaat tttatatgaa
     8341 cgtatgaaag ctctgggtcc tgatgtgcca gaactcctta ttctccccgt ttattcggcc
     8401 ctgccgagcg aaatgcagag tcgaatcttc gaaccagcgc cgccgggaac cagaaaagtc
     8461 gtcattgcga caaatatcgc cgagacctcc attaccattg acgacatcta ctttgtcgta
     8521 gatccaggct tcgtcaaaca aaacgcgtat gatccaaagc tcggtatgga ctcgcttgtt
     8581 gtcacaccaa tttcacaggc ccaggcaaac cagcgagcag gtcgagccgg ccgaactggg
     8641 cccggaaaat gcttcaggtt atacaccgag gcagcgtacc aaaccgagat gctgccgacg
     8701 acgatcccag aaatccagcg gcagaacttg tcgttaacta ttctcatgct caaagccatg
     8761 ggcatcaacg atcttatcaa ctttgacttc atggacccgc ctcccgttaa cactatgctt
     8821 acagccctcg aggagctgta tgcgctcagc gctctcgacg acgagggcct gcttacccgc
     8881 ctcggacgca agatggccga ctttcctatg gagccgtcac tcgccaaagt cctaatcgcc
     8941 tcggtcgatc tgggctgctc cgacgaaatg ctcagcatag tagcgatgct cggcatgaac
     9001 gtcttctacc ggcccaagga gaagcagacc caggccgacc agaagaaggc caagttccac
     9061 gatccgcacg gcgaccacct gaccctgctc aacgtctaca acagctggaa gcgcagcggg
     9121 tatcagaacg cctggtgctt cgagaactac atccagcacc ggtccatgaa gcgctccaag
     9181 gacgtgcgcg accagctcgt caaaatcatg gagcgctacc gccatcccat cgtctcgtgc
     9241 ggccgcgaca cacacaaggt gcggcaggct ctctgctccg gcttcttccg ccactcggcg
     9301 cgtaaggacc cccaggaagg ctacaagacc ctcatcgagg gcacccccgt gtacctgcat
     9361 cccagctcgg cgctgttcgg caagcaggcc gagtgggtca tctaccatga gctcgtgctg
     9421 acaacgaagg agtacatgca ttggacgacg gtcatcgagc ccaagtggtt ggtggaggcg
     9481 gcgccgacgt ttttcaaggt tgcgccgacg gataggctgt caaagaggaa gcaggctgag
     9541 aggatccagc cgttgtttaa taggtttgag ggaccggatg attggaggct tagtgcgcag
     9601 aggagggttg gaaggtctgg tggtggtgga ggtacttggg gttagagtgc ttttaactaa
     9661 gaattattag tcttttctgc ttattttttc atcatagttt agaacacttt atattaacga
     9721 atagtttatg aatctattta ggtttaaaaa ttgatacagt tttataagtt actttttcaa
     9781 agactcgtgc tgtctattgc ataatgcact ggaaggagaa aaaaaaggtg cacacgcgtg
     9841 gctttttctt gaatttgcag tttgaaaaat aactacatgg atgataagaa aacatggagt
     9901 acagtcactt tgagaacctt caatcagctg gtaacgtctt cgttaattgg atactcaaaa
     9961 aagatggata gcatgaatca caagatggaa ggaaatgcgg gccacgacca cagtgatatg
    10021 catatgggag atggagatga tacctccatt gggccgatga agttagtcga cggatagaag
    10081 cggttgtccc ctttcccggc gagccggcag tcgggccgag gttcggataa attttgtatt
    10141 gtgttttgat tctgtcatga gtattactta tgttctcttt aggtaacccc aggttaatca
    10201 atcacagttt cataccggct agtattcaaa ttatgacttt tcttctgcag tgtcagcctt
    10261 acgacgatta tctatgagct ttgaatatag tttgccgtga ttcgtatctt taattggata
    10321 ataaaatgcg aaggatcgat gacccttatt attatttttc tacactggct accgatttaa
    10381 ctcatcttct tgaaagtata taagtaacag taaaatatac cgtacttctg ctaatgttat
    10441 ttgtccctta tttttctttt cttgtcttat gctatagtac ctaagaataa cgactattgt
    10501 tttgaactaa acaaagtagt aaaagcacat aaaagaatta agaaaatggg ctccatgccg
    10561 aataactcag aaccaatatc agcgatcctt gatgccatcg gtcatgtcac gtcctctctg
    10621 gccgcctacg agcgggtaac gaggaagtac ggtggtagtg aagaagttgc ccataagggc
    10681 gagattgcag ttgcacgcaa tgccatgacc aaggatgcta tccagcttct ccaagaaatt
    10741 cgtgggccca ttgataccgt ttatggattc ttcgaaatgg gatcccaagc atcggctatt
    10801 cgctgcctca ttgcaatggg agcgtttgac aagattccca ctgaaggcag catcactgta
    10861 gccaagctgg catccgagct cggtgttgac cagaacctgc tcacccgttt gatgagagtt
    10921 gtgagcgtca ccggaccctt ccaacaagtg cgagaggatg agtatgccca tactgctttc
    10981 tccaaggctt atctgatccc agaagtccga ggaatgtata ctctgatatt cgacgaaatg
    11041 taccccccgt gggtcaaaat gcacgagttt ctcgcctctc gcggttggaa gagtgatggg
    11101 gaggagagag ataatgcata cacgtatgcg catcaaaccg gcggcaagag cttctgggag
    11161 catctgaagc agtatccaaa gcgtgtggag gctctcaacc tcggtatggg agcacagact
    11221 gctacaacca tgtggtccgt ggacattgtc gacttcaggg ccctgctggc atccaagaag
    11281 acaaacaagg atgacgtgtt ggtagtcgac gttggaggtg ggaagggaca ctgccttcag
    11341 aggatcaaca aggctatctc ggacatcgag ggccgcctgg tcctgcaaga tcgtccagag
    11401 gtgattagcg atacgtacga cttggattct tcgcggatcg agaagtacga gtacaacttc
    11461 tttgaacctc agccaatcca gggcgcgcat atatactaca tccgtcgcgt cttgcatgac
    11521 tggccgaatc atctttgcgt cgacatcctg aagaacattg ccaatgcgat ggagcgcgga
    11581 aagtcgcgtt tggtcatcgc agaaatcgtt gttcccccaa ctggagggga ttcggaaact
    11641 gggtggatgg atctagttct aatgactgtc actggtgtcg agcgcagcgc aaaagactgg
    11701 caagaattac tcagtgccgc aggtctcaag ctggagaaga tctatacttc ccctggcact
    11761 aaccacggtg ctgtccaagc cgtccttgca tgaggagatt gataagactt ttctagttgc
    11821 atatctttta tatttaaatc ttatctatta gttaattttt tgtaatttat ccttatatat
    11881 agtctggtta ttctaaaata tcatttcagt atctaaaaat tcccctcttt tttcagttat
    11941 atcttaacag gcgacagtcc aaatgttgat ttatcccagt ccgattcatc agggttgtga
    12001 agcattttgt caatggtcga aatcacatca gtaatagtgc ctcttacttg cctcatagaa
    12061 tttctttctc ttaacgtcac cgtttggtct tttatagttt cgaaatctat ggtgatacca
    12121 aatggtgttc ccaattcatc gttacgggcg tattttttac caattgaagt attggaatcg
    12181 tcaattttaa agtatatctc tcttttacgt aaagcctgcg agatcctctt aagtatagcg
    12241 gggaagccat cgttattcga tattgtcgta acaaatactt tgatcggcgc tatgcggccg
    12301 ccaccgcggt ggagctccag cttttgttcc ctttagtgag ggttaattgc gcgcttggcg
    12361 agactcaaac aagatatatt ttgcccgcct tacagacgaa actagttgga ggttatggag
    12421 catactatca cgtgggcggc cactggtgag ttactacacc ccaggggcaa cgttgatgct
    12481 cctaaaaaac tctggctgga cgcaagccgt aacacccgtg tcacttcata atcgtttgca
    12541 attcagggct tgatctacac attaccctgt tatccctatt aattaatacg gttatccaca
    12601 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
    12661 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
    12721 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
    12781 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
    12841 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
    12901 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
    12961 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
    13021 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
    13081 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
    13141 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
    13201 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
    13261 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
    13321 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
    13381 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
    13441 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
    13501 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
    13561 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
    13621 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
    13681 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
    13741 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
    13801 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
    13861 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
    13921 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
    13981 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
    14041 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
    14101 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
    14161 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
    14221 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
    14281 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
    14341 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
    14401 ggggttccgc gcacatttcc ccgaaaagtg ccacctgaac gaagcatctg tgcttcattt
    14461 tgtagaacaa aaatgcaacg cgagagcgct aatttttcaa acaaagaatc tgagctgcat
    14521 ttttacagaa cagaaatgca acgcgaaagc gctattttac caacgaagaa tctgtgcttc
    14581 atttttgtaa aacaaaaatg caacgcgaga gcgctaattt ttcaaacaaa gaatctgagc
    14641 tgcattttta cagaacagaa atgcaacgcg agagcgctat tttaccaaca aagaatctat
    14701 acttcttttt tgttctacaa aaatgcatcc cgagagcgct atttttctaa caaagcatct
    14761 tagattactt tttttctcct ttgtgcgctc tataatgcag tctcttgata actttttgca
    14821 ctgtaggtcc gttaaggtta gaagaaggct actttggtgt ctattttctc ttccataaaa
    14881 aaagcctgac tccacttccc gcgtttactg attactagcg aagctgcggg tgcatttttt
    14941 caagataaag gcatccccga ttatattcta taccgatgtg gattgcgcat actttgtgaa
    15001 cagaaagtga tagcgttgat gattcttcat tggtcagaaa attatgaacg gtttcttcta
    15061 ttttgtctct atatactacg tataggaaat gtttacattt tcgtattgtt ttcgattcac
    15121 tctatgaata gttcttacta caattttttt gtctaaagag taatactaga gataaacata
    15181 aaaaatgtag aggtcgagtt tagatgcaag ttcaaggagc gaaaggtgga tgggtaggtt
    15241 atatagggat atagcacaga gatatatagc aaagagatac ttttgagcaa tgtttgtgga
    15301 agcggtattc gcaatatttt agtagctcgt tacagtccgg tgcgtttttg gttttttgaa
    15361 agtgcgtctt cagagcgctt ttggttttca aaagcgctct gaagttccta tactttctag
    15421 agaataggaa cttcggaata ggaacttcaa agcgtttccg aaaacgagcg cttccgaaaa
    15481 tgcaacgcga gctgcgcaca tacagctcac tgttcacgtc gcacctatat ctgcgtgttg
    15541 cctgtatata tatatacatg agaagaacgg catagtgcgt gtttatgctt aaatgcgtac
    15601 ttatatgcgt ctatttatgt aggatgaaag gtagtctagt acctcctgtg atattatccc
    15661 attccatgcg gggtatcgta tgcttccttc agcactaccc tttagctgtt ctatatgctg
    15721 ccactcctca attggattag tctcatcctt caatgctatc atttcctttg atattggatc
    15781 atactaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
    15841 ctttcgtctc gcgcgtttcg gtgatgacgg tgagcgcgtt tcggtgatga cggtgaaaac
    15901 ctcggcgcgc cattaccctg ttatccctaa tatacaacgc aaagaagctg gaaaattggc
    15961 gagggaatcc tgtttctgtc tatccaagaa tgggcatgag gtggcaaccg tcgtgctagc
    16021 gtacagggtg cactttgtaa ccatttggga caccggacac tcgctgtttt cgaaattacc
    16081 ctttaagcgc gggtattgaa ccaggcttat gcccaagatc gtagcaagca tttaaatgac
    16141 ggtatcgata agcttgatat cgaattcctg cagcccgggg gatccactag ttctagatta
    16201 attaatatct aaaaattgcc ttatgatccg tctctccggt tacagcctgt gtaactgatt
    16261 aatcctgcct ttctaatcac cattctaatg ttttaattaa gggattttgt cttcattaac
    16321 ggctttcgct cataaaaatg ttatgacgtt ttgcccgcag gcgggaaacc atccacttca
    16381 cgagactgat ctcctctgcc ggaacaccgg gcatctccaa cttataagtt ggagaaataa
    16441 gaga
//
