LOCUS       sequence-211447-       13620 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  HIVGKO contains a codon-switched eGFP under the control of the HIV-1
            specific promoter, and mKO2 under the control of the constitutive 
            promoter EF1a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 13620)
  AUTHORS   Battivelli E, Dahabieh MS, Abdel-Mohsen M, Svensson JP, Tojal Da 
            Silva I, Cohn LB, Gramatica A, Deeks S, Greene WC, Pillai SK, Verdin
            E
  TITLE     Distinct chromatin functional states correlate with HIV latency 
            reactivation in infected primary CD4(+) T cells.
  JOURNAL   Elife. 2018 May 1;7. pii: 34655. doi: 10.7554/eLife.34655.
  PUBMED    29714165
REFERENCE   2  (bases 1 to 13620)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-05-1"; volume: "7. pii: 34655. doi"; pages: " 
            10.7554/eLife.34655"
COMMENT     Sequence Label: HIVGKO
FEATURES             Location/Qualifiers
     source          1..13620
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..17
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1..17
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     LTR             48..681
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    728..853
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4864..4981
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    7863..8096
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8281..8325
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             8619..8645
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             join(8892..8894,8895..8897,8898..9608)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     9545..9566
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        9679..9890
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             9903..10171
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      10209..10218
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             10215..10865
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAH"
     LTR             11065..11697
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(11730..11748)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11816..11920
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11921..11989,11990..12781)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12139..12158)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12952..13540
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13441..13460
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tctagaacta gtggatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
       61 tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac aaggctactt
      121 ccctgattgg cagaactaca caccagggcc aggggtcaga tatccactga cctttggatg
      181 gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata aaggagagaa
      241 caccagcttg ttacaccctg tgagcctgca tggaatggat gaccctgaga gagaagtgtt
      301 agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc tgcatccgga
      361 gtacttcaag aactgctgac atcgagcttg ctacaaggga ctttccgctg gggactttcc
      421 agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatgct gcatataagc
      481 agctgctttt tgcctgtact gggtctctct ggttagacca gatttgagcc tgggagctct
      541 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
      601 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
      661 cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc
      721 agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg
      781 gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg
      841 gtgcgagagc gtcggtatta agcgggggag aattagataa atgggaaaaa attcggttaa
      901 ggccaggggg aaagaaacaa tataaactaa aacatatagt atgggcaagc agggagctag
      961 aacgattcgc agttaatcct ggccttttag agacatcaga aggctgtaga caaatactgg
     1021 gacagctaca accatccctt cagacaggat cagaagaact tagatcatta tataatacaa
     1081 tagcagtcct ctattgtgtg catcaaagga tagatgtaaa agacaccaag gaagccttag
     1141 ataagataga ggaagagcaa aacaaaagta agaaaaaagc acagcaagca gcagctgaca
     1201 caggacacag cagccaggtc agccaaaatt accctatagt gcagaacatc caggggcaaa
     1261 tggtacatca ggccatatca cctagaactt taaatgcatg ggtaaaagta gtagaagaga
     1321 aggctttcag cccagaagtg atacccatgt tttcagcatt atcagaagga gccaccccac
     1381 aagatttaaa caccatgcta aacacagtgg ggggacatca agcagccatg caaatgttaa
     1441 aagagaccat caatgaggaa gctgcagaat gggatagagt gcatccagtg catgcagggc
     1501 ctattgcacc aggccagatg agagaaccaa ggggaagtga catagcagga actactagta
     1561 cccttcagga acaaatagga tggatgacac ataatccacc tatcccagta ggagaaatct
     1621 ataaaagatg gataatcctg ggattaaata aaatagtaag aatgtatagc cctaccagca
     1681 ttctggacat aagacaagga ccaaaggaac cctttagaga ctatgtagac cgattctata
     1741 aaactctaag agccgagcaa gcttcacaag aggtaaaaaa ttggatgaca gaaaccttgt
     1801 tggtccaaaa tgcgaaccca gattgtaaga ctattttaaa agcattggga ccaggagcga
     1861 cactagaaga aatgatgaca gcatgtcagg gagtgggggg acccggccat aaagcaagag
     1921 ttttggctga agcaatgagc caagtaacaa atccagctac cataatgata cagaaaggca
     1981 attttaggaa ccaaagaaag actgttaagt gtttcaattg tggcaaagaa gggcacatag
     2041 ccaaaaattg cagggcccct aggaaaaagg gctgttggaa atgtggaaag gaaggacacc
     2101 aaatgaaaga ttgtactgag agacaggcta attttttagg gaagatctgg ccttcctaca
     2161 agggaaggcc agggaatttt cttcagagca gaccagagcc aacagcccca ccatttcttc
     2221 agagcagacc agagccaaca gccccaccag aagagagctt caggtctggg gtagagacaa
     2281 caactccctc tcagaagcag gagccgatag acaaggaact gtatccttta acttccctca
     2341 gatcactctt tggcaacgac ccctcgtcac aataaagata ggggggcaac taaaggaagc
     2401 tctattagat acaggagcag atgatacagt attagaagaa atgagtttgc caggaagatg
     2461 gaaaccaaaa atgatagggg gaattggagg ttttatcaaa gtaagacagt atgatcagat
     2521 actcatagaa atctgtggac ataaagctat aggtacagta ttagtaggac ctacacctgt
     2581 caacataatt ggaagaaatc tgttgactca gattggttgc actttaaatt ttcccattag
     2641 tcctattgaa actgtaccag taaaattaaa gccaggaatg gatggcccaa aagttaaaca
     2701 atggccattg acagaagaaa aaataaaagc attagtagaa atttgtacag aaatggaaaa
     2761 ggaagggaaa atttcaaaaa ttgggcctga aaatccatac aatactccag tatttgccat
     2821 aaagaaaaaa gacagtacta aatggagaaa attagtagat ttcagagaac ttaataagag
     2881 aactcaagac ttctgggaag ttcaattagg aataccacat cccgcagggt taaaaaagaa
     2941 aaaatcagta acagtactgg atgtgggtga tgcatatttt tcagttccct tagatgaaga
     3001 cttcaggaag tatactgcat ttaccatacc tagtataaac aatgagacac cagggattag
     3061 atatcagtac aatgtgcttc cacagggatg gaaaggatca ccagcaatat tccaaagtag
     3121 catgacaaaa atcttagagc cttttagaaa acaaaatcca gacatagtta tctatcaata
     3181 catggatgat ttgtatgtag gatctgactt agaaataggg cagcatagaa caaaaataga
     3241 ggagctgaga caacatctgt tgaggtgggg acttaccaca ccagacaaaa aacatcagaa
     3301 agaacctcca ttcctttgga tgggttatga actccatcct gataaatgga cagtacagcc
     3361 tatagtgctg ccagaaaaag acagctggac tgtcaatgac atacagaagt tagtgggaaa
     3421 attgaattgg gcaagtcaga tttacccagg gattaaagta aggcaattat gtaaactcct
     3481 tagaggaacc aaagcactaa cagaagtaat accactaaca gaagaagcag agctagaact
     3541 ggcagaaaac agagagattc taaaagaacc agtacatgga gtgtattatg acccatcaaa
     3601 agacttaata gcagaaatac agaagcaggg gcaaggccaa tggacatatc aaatttatca
     3661 agagccattt aaaaatctga aaacaggaaa atatgcaaga acgaggggtg cccacactaa
     3721 tgatgtaaaa caattaacag aggcagtgca aaaaataacc acagaaagca tagtaatatg
     3781 gggaaagact cctaaattta aactacccat acaaaaggaa acatgggaaa catggtggac
     3841 agagtattgg caagccacct ggattcctga gtgggagttt gtcaataccc ctcctttagt
     3901 gaaattatgg taccagttag agaaagaacc catagtagga gcagaaacgt tctatgtaga
     3961 tggggcagct agcagggaga ctaaattagg aaaagcagga tatgttacta atagaggaag
     4021 acaaaaagtt gtcaccctaa ctgacacaac aaatcagaag actgagttac aagcaattca
     4081 tctagctttg caggattcgg gattagaagt aaatatagta acagactcac aatatgcatt
     4141 aggaatcatt caagcacaac cagataaaag tgaatcagag ttagtcaatc aaataataga
     4201 gcagttaata aaaaaggaaa aggtctatct ggcatgggta ccagcacaca aaggaattgg
     4261 aggaaatgaa caagtagata aattagtcag tgctggaatc aggaaagtac tatttttaga
     4321 tggaatagat aaggcccaag atgaacatga gaaatatcac agtaattgga gagcaatggc
     4381 tagtgatttt aacctgccac ctgtagtagc aaaagaaata gtagccagct gtgataaatg
     4441 tcagctaaaa ggagaagcca tgcatggaca agtagactgt agtccaggaa tatggcaact
     4501 agattgtaca catttagaag gaaaagttat cctggtagca gttcatgtag ccagtggata
     4561 tatagaagca gaagttattc cagcagaaac agggcaggaa acagcatact ttcttttaaa
     4621 attagcagga agatggccag taaaaacaat acatacagac aatggcagca atttcaccag
     4681 tactacggtt aaggccgcct gttggtgggc gggaatcaag caggaatttg gaattcccta
     4741 caatccccaa agtcaaggag tagtagaatc tatgaataaa gaattaaaga aaattatagg
     4801 ccaggtaaga gatcaggctg aacatcttaa gacagcagta caaatggcag tattcatcca
     4861 caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     4921 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     4981 tcgggtttat tacagggaca gcagagatcc actttggaaa ggaccagcaa agctcctctg
     5041 gaaaggtgaa ggggcagtag taatacaaga taatagtgac ataaaagtag tgccaagaag
     5101 aaaagcaaag atcattaggg attatggaaa acagatggca ggtgatgatt gtgtggcaag
     5161 tagacaggat gaggattaga acatggaaaa gtttagtaaa acaccatatg tatgtttcag
     5221 ggaaagctag gggatggttt tatagacatc actatgaaag ccctcatcca agaataagtt
     5281 cagaagtaca catcccacta ggggatgcta gattggtaat aacaacatat tggggtctgc
     5341 atacaggaga aagagactgg catctgggtc agggagtctc catagaatgg aggaaaaaga
     5401 gatatagcac acaagtagac cctgaactag cagaccaact aattcatctg tattactttg
     5461 actgtttttc agactctgct ataagaaagg ccttattagg acatatagtt agccctaggt
     5521 gtgaatatca agcaggacat aacaaggtag gatctctaca atacttggca ctagcagcat
     5581 taataacacc aaaaaagata aagccacctt tgcctagtgt tacgaaactg acagaggata
     5641 gatggaacaa gccccagaag accaagggcc acagagggag ccacacaatg aatggacact
     5701 agagctttta gaggagctta agaatgaagc tgttagacat tttcctagga tttggctcca
     5761 tggcttaggg caacatatct atgaaactta tggggatact tgggcaggag tggaagccat
     5821 aataagaatt ctgcaacaac tgctgtttat ccatttcaga attgggtgtc gacatagcag
     5881 aataggcgtt actcaacaga ggagagcaag aaatggagcc agtagatcct agactagagc
     5941 cctggaagca tccaggaagt cagcctaaaa ctgcttgtac cacttgctat tgtaaaaagt
     6001 gttgctttca ttgccaagtt tgtttcacaa caaaagcctt aggcatctcc tatggcagga
     6061 agaagcggag acagcgacga agacctcctc aaggcagtca gactcatcaa gtttctctat
     6121 caaagcagta agtagtacat gtaatgcaac ctatacaaat agcaatagca gcattagtag
     6181 tagcaataat aatagcaata gttgtgtggt ccatagtaat catagaatat aggaaaatat
     6241 taagacaaag aaaaatagac aggttaattg atagactaat agaaagagca gaagacagtg
     6301 gcaatgagag tgaaggagaa atatcagcac ttgtggagat gggggtggaa atggggcacc
     6361 atgctccttg ggatattgat gatctgtagt gctacagaaa aattgtgggt cacagtctat
     6421 tatggggtac ctgtgtggaa ggaagcaacc accactctat tttgtgcatc agatgctaaa
     6481 gcatatgata cagaggtaca taatgtttgg gccacacatg cctgtgtacc cacagacccc
     6541 aacccacaag aagtagtatt ggtaaatgtg acagaaaatt ttaacatgtg gaaaaatgac
     6601 atggtagaac agatgcatga ggatataatc agtttatggg atcaaagcct aaagccatgt
     6661 gtaaaattaa ccccactctg tgttagttta aagtgcactg atttggggaa tgctactaat
     6721 accaatagta gtaataccaa tagtagtagc ggggaaatga tgatggagaa aggagagata
     6781 aaaaactgct ctttcaatat cagcacaagc ataagaggta aggtgcagaa agaatatgca
     6841 tttttttata aacttgatat aataccaata gataatgata ctaccagcta tacgttgaca
     6901 agttgtaaca cctcagtcat tacacaggcc tgtccaaagg tatcctttga gccaattccc
     6961 atacattatt gtgccccggc tggttttgcg attctaaaat gtaataataa gacgttcaat
     7021 ggaacaggac catgtacaaa tgtcagcaca gtacaatgta cacatggaat taggccagta
     7081 gtatcaactc aactgctgtt gaatggcagt ctagcagaag aagaggtagt aattagatct
     7141 gccaatttca cagacaatgc taaaaccata atagtacagc tgaaccaatc tgtagaaatt
     7201 aattgtacaa gacccaacaa caatacaaga aaaagtatcc gtatccagag gggaccaggg
     7261 agagcatttg ttacaatagg aaaaatagga aatatgagac aagcacattg taacattagt
     7321 agagcaaaat ggaatgccac tttaaaacag atagctagca aattaagaga acaatttgga
     7381 aataataaaa caataatctt taagcaatcc tcaggagggg acccagaaat tgtaacgcac
     7441 agttttaatt gtggagggga atttttctac tgtaattcaa cacaactgtt taatagtact
     7501 tggtttaata gtacttggag tactgaaggg tcaaataaca ctgaaggaag tgacacaatc
     7561 acactcccat gcagaataaa acaatttata aacatgtggc aggaagtagg aaaagcaatg
     7621 tatgcccctc ccatcagcgg acaaattaga tgttcatcaa atattacagg gctgctatta
     7681 acaagagatg gtggtaataa caacaatggg tccgagatct tcagacctgg aggaggagat
     7741 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     7801 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     7861 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     7921 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     7981 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     8041 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     8101 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     8161 agtaataaat ctctggaaca gatttggaat aacatgacct ggatggagtg ggacagagaa
     8221 attaacaatt acacaagctt aatacattcc ttaattgaag aatcgcaaaa ccagcaagaa
     8281 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     8341 ataacaaatt ggctgtggta tataaaaata ttcataatga tagtaggagg cttggtaggt
     8401 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     8461 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     8521 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atccttggca
     8581 cttatctggg acgatctgcg gagcctgtgc ctcttcagct accaccgctt gagagactta
     8641 ctcttgattg taacgaggat tgtggaactt ctgggacgca gggggtggga agccctcaaa
     8701 tattggtgga atctcctaca atattggagt caggagctaa agaatagtgc tgttagcttg
     8761 ctcaatgcca cagccatagc agtagctgag gggacagata gggttataga agtagtacaa
     8821 ggagcttgta gagctattcg ccacatacct agaagaataa gacagggctt ggaaaggatt
     8881 ttgctataag catggtctcc aaaggggaag agctgttcac cggcgtggtg cccatcctgg
     8941 tcgagctgga tggggatgtg aatgggcata aatttagcgt gtccggggaa ggggaagggg
     9001 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctccctgtcc
     9061 cttggcccac cctcgtgacc accctgactt atggcgtgca gtgcttcagc cgctaccctg
     9121 accatatgaa acagcacgat ttcttcaagt ccgccatgcc tgaggggtat gtgcaggaac
     9181 ggactatctt tttcaaagac gatgggaatt ataagacccg ggccgaagtc aagttcgagg
     9241 gcgacacact cgtgaaccgc atcgaactca aagggattga ttttaaggag gatgggaata
     9301 ttctggggca caaactggag tacaactaca acagccacaa cgtctatatc atggccgaca
     9361 agcagaagaa cggcattaaa gtgaacttca agatccgcca caacatcgaa gatggcagcg
     9421 tccagctcgc cgatcattat caacagaaca cccccatcgg cgacgggcct gtcctcctcc
     9481 ctgataacca ctacctgagc acccagtccg ccctgagcaa agaccctaat gaaaagcgcg
     9541 atcacatggt cctgctggag ttcgtgacag ctgctggcat cactctgggg atggatgaac
     9601 tctataaata aagcgaccct cgagtactag gatcgattag gcggccgcgg atctgcgatc
     9661 gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg
     9721 gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg
     9781 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag
     9841 tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag ctgaagcttc
     9901 gaggggctcg catctctcct tcacgcgccc gccgccctac ctgaggccgc catccacgcc
     9961 ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg tccgccgtct
    10021 aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct tggagcctac
    10081 ctagactcag ccggctctcc acgctttgcc tgaccctgct tgctcaactc tacgtctttg
    10141 tttcgttttc tgttctgcgc cgttacagat ccaagctgtg accggcgcct acgctagcgc
    10201 taccggtcgc caccatggtt tctgtgatca agcccgaaat gaagatgagg tactacatgg
    10261 acggcagcgt caatggacat gagtttacga tcgaagggga gggcaccggc cgaccctacg
    10321 agggccacca ggagatgacc ctgagggtga cgatggcaga gggcggtccc atgcccttcg
    10381 ccttcgacct ggtaagccac gtgttctgct acggccaccg agtgttcaca aaatacccgg
    10441 aggagatccc agactacttc aagcaggcct ttcccgaggg gctgagctgg gagaggagcc
    10501 tggaatttga ggacggtggc agtgctagcg ttagcgccca catcagtctg aggggcaaca
    10561 ccttttacca caagagcaag ttcactggcg ttaacttccc agcggacggc cccatcatgc
    10621 agaaccagag cgtggactgg gagcccagca ccgagaagat caccgccagc gacggcgtgc
    10681 tgaagggcga cgtgaccatg tacctgaagc tggaaggagg tggcaaccat aagtgccaga
    10741 tgaagaccac ctacaaggcc gccaaggaga ttctggagat gccgggagac cactatattg
    10801 gacacaggct ggtccgaaag actgaaggta acatcaccga acaggtggaa gacgccgtgg
    10861 cccactacag ctaggctcga gacctggaaa aacatggagc aatcacaagt agcaatacag
    10921 cagctaccaa tgctgcttgt gcctggctag aagcacaaga ggaggaggag gtgggttttc
    10981 cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
    11041 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagata
    11101 tccttgatct gtggatctac cacacacaag gctacttccc tgattggcag aactacacac
    11161 cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
    11221 agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga
    11281 gcctgcatgg aatggatgac cctgagagag aagtgttaga gtggaggttt gacagccgcc
    11341 tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgactcg
    11401 agcttgctac aagggacttt ccgctgggga ctttccaggg aggcgtggcc tgggcgggac
    11461 tggggagtgg cgagccctca gatgctgcat ataagcagct gctttttgcc tgtactgggt
    11521 ctctctggtt agaccagatt tgagcctggg agctctctgg ctaactaggg aacccactgc
    11581 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
    11641 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg
    11701 gcgatgaatt aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa
    11761 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
    11821 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
    11881 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
    11941 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
    12001 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
    12061 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
    12121 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga
    12181 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
    12241 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
    12301 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
    12361 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
    12421 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac
    12481 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
    12541 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
    12601 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
    12661 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
    12721 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
    12781 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
    12841 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
    12901 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
    12961 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
    13021 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
    13081 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
    13141 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
    13201 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
    13261 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
    13321 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
    13381 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
    13441 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
    13501 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
    13561 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatccgc
//
