LOCUS       sequence-211461-        7543 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pRepCap6 contains both rep and cap genes from the AAV6 genome, but 
            lacks the viral terminal repeats (Rutledge et al., 1998). It lacks 
            adenovirus helper functions required for stock production..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7543)
  AUTHORS   Rutledge EA, Halbert CL, Russell DW
  TITLE     Infectious clones and vectors derived from adeno-associated virus 
            (AAV) serotypes other than AAV type 2.
  JOURNAL   J Virol. 1998 Jan;72(1):309-19.
  PUBMED    9420229
REFERENCE   2  (bases 1 to 7543)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol."; 
            date: "1998-01"; volume: "72(1)"; pages: "309-19"
COMMENT     Sequence Label: pRepCap6
FEATURES             Location/Qualifiers
     source          1..7543
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     68..86
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(124..146)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     246..265
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(332..787)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(469..490)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     681..700
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     913..935
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     927..944
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     928..944
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     951..970
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        951..969
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(1258..1269)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     promoter        complement(5378..5396)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5417..5433)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5417..5433)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5430..5452)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5441..5457
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5465..5471,5472..5489,5490..5495))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5510..5531
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5648..5665)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5819..6407)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5899..5918)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6578..7369,7370..7438))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7201..7220
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7439..7543)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
       61 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga
      121 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
      181 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
      241 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
      301 cagatgcgta aggagaaaat accgcatcag gaaattgtaa gcgttaatat tttgttaaaa
      361 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa
      421 atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac
      481 aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag
      541 ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt
      601 aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg
      661 gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca
      721 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
      781 ggcgcgtcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
      841 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
      901 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgaattg taatacgact
      961 cactataggg cgaattgggc ccaatgcatt ggcgccgcgg ccgcgaattc gagctcggta
     1021 cccggggatc tggtgaccag ataagataat aacgacatga gaccaaagtt caactgacac
     1081 gaattaaccg gtttattgat taacagacaa ttacagggga cgggtgaggt aacgggtgcc
     1141 aatggggcga ggctcagtat aaagtccatt gttgtccaca gtgaaatcaa cgttggcaga
     1201 ttttgcatag ttagatgtat actgcacttc gggattccag cgtttgctgt tttctttctg
     1261 cagctcccat tcaatctcca cgctcacttg tcctgtggaa tactgggtga tgaatgaagc
     1321 aaactttgta gccgaaaact ctgccggagg attcgcagga acaggcgtgt ttttgatgag
     1381 gatctgagga ggcgggtgct taagtccaaa gccgcccatg agaggagacg ggtgaaagtg
     1441 tccatccgtg tgaggaattt tggcccaaat aggaccctgc aggtatacgt ctctgtcttg
     1501 ccacaccatt ccaggtaagg ctcccataac atgcacatct ccggtcgcag ggtctgtgct
     1561 gctgctctgg agattgactg ccacagtccc aaatctttcg gtggccacgg ggttagtggc
     1621 tttgatttcc tcttcgtctg tgatcatgac attgtccaat gcagtgtttg aagctccggc
     1681 gctctccttt ccaaaaatca tgacaccgct catgggaaag aacttgtctt tgtcgtcttt
     1741 gtgtgaggcc atagcagtgc cagggttgat tatagattca cgcccattaa ggttatattt
     1801 tgaagcacca gtccaggtaa agttgctgtt gttgttgtct gtttttgttt tagaaacgcg
     1861 ctgctgccgg taacagggtc caggtagcca gtttttgggc tgaacagaca tgccagctgg
     1921 agacccccgg ctaaacagca agtccttgtt ttgggcactt ccggactgat tctgagttct
     1981 gttcaggtaa tacaggtact ggtcgatgag aggattcatc agccggtcca ggctctggct
     2041 gtgcgcgtag ctgctgtgga aaggcacgtc ctcgaaggtg tagctgaagg taaagttatt
     2101 gcccgttctc agcatctgcg atgggaaata ttccaggcag taaaaggatg accgtcccac
     2161 tgcctggctg ccattgttga gcgttaggta gccgtactgc ggaatcatga acacgtccgc
     2221 cgggaacgga gggaggcagc cctggtgcgc agagccgagg acgtacggca actggtactc
     2281 cgagtccgag aagacttgaa ccgtgctggt aaggttatta gcgatggtcg tgacgccatc
     2341 attcgtcgtg acctccttga cttggatgtt gaagagcttg aagttgagtc tcttgggccg
     2401 gaatccccaa ttgttgttga tgagtcgctg ccagtcacgt ggtgagaaat ggcagtggaa
     2461 tctgttgaaa tcaaaatacc cccagggggt gctgtagccg aagtagtggt tgtcgttgct
     2521 ggcccccgtt gaagcactgg agatttgctt gtagaggtgg ttgttatagg tgggcaaggc
     2581 ccatgttcgg gtgctggtgg tgatgactct gtcgcccagc catgtggaat cgcaatgcca
     2641 atttcctgag gcattaccca ctccgtcggc gccttcgtta ttgtctgcca ttggtgcgcc
     2701 accgcctgaa gccattgtag taggtcccac agcagcgggg gttgctggag gttctccgag
     2761 aggttgtggg tcggggactg actctgagtc gccagtctga ccaaaattga gtctcttttt
     2821 agcgggctgc tggcctgtct tgccaatgcc cgaggaggag tctggctctt gtggcgactg
     2881 ctctaccgga cgtttctttc caggagccgt cttagcacct tcctcaacca gaccaaaagg
     2941 ttcgagaacc ctcttcttgg cctggaagac tgctcgcccg aggttgcccc caaaagacgt
     3001 atcttcttgc agacgctcct gaaactcggc gtcggcgtgg ttataccgca ggtacggatt
     3061 gtcacccgct ttgagctgct ggtcgtaggc cttgtcgtgc tcgagggccg ctgcatccgc
     3121 cgcgttgacg ggctccccct tgtcgagtcc gttgaagggt ccgaggtact tgtagccagg
     3181 aagcaccaga ccccggccgt cgtcctgctt ttgctggttg gctttgggtt tcggggctcc
     3241 aggtttcaag tcccaccact cgcgaatgcc ctcagagagg ttgtcctcga gccaatctgg
     3301 aagataacca tcggcagcca tacctggttt aagtcattta ttgctcagaa acacagtcat
     3361 ccagatccac gttgaccaga tcgcaggccg agcaagcaat ctcgggagcc cgccccagca
     3421 gatgatgaat ggcacagagt ttccgatacg tcctctttct gacgaccggt tgagattctg
     3481 acacgccggg gaaacattct gaacagtctc tggtcccgtg cgtgaagcaa atgttgaaat
     3541 tctgattcat tctctcgcat gttttgcagg gaaacagcat ctgaagcatg cccgcgtgac
     3601 gagaacattt gttttggtac ctgtcggcaa agtccaccgg agctccttcc gcgtctgacg
     3661 tcgatggatc cgcgactgag gggcaggccc gcttgggctc gcttttatcc gcgtcatcgg
     3721 gggcgggtct cttgttggct ccaccctttc tgacgtagaa ctcatgcgcc acctcggtca
     3781 cgtgatcctg cgcccagcgg aagaactctt tgacttcctg ctttgtcacc ttgccaaagt
     3841 catgctccag acggcgggtg agttcaaatt tgaacatccg gtcctgcaac ggctgctggt
     3901 gctcgaaggt ggtgctgttc ccgtcaatca cggcgcacat gttggtgttg gaggtgacga
     3961 tcacgggggt gggatcgatc tgggcggacg acttgcactt ttggtccacg cgcaccttgc
     4021 tgccgccgag aatggccttg gcggactcca cgaccttggc cgtcatcttg ccctcctccc
     4081 accagatcac catcttgtcg acgcaatcgt tgaagggaaa gttctcattg gtccagttga
     4141 cgcagccgta gaagggcacg gcgtgggcga tggcttccgc gatgttggtc ttgcccgtgg
     4201 tggccggccc aaacagccag atggtgttgc gttttccgaa ccttttctgg gcccagccga
     4261 gaaagacgga gccggcgtag gcagggtcgt agccgttcag ctccaggatg cggtaaatgc
     4321 ggttggtttt aatgtcggcg ggcggagcgg ggcctaccag gtagtcgggc gcggatttgg
     4381 tcagcgccat gatcttgccg gcattgtcca gagcggcctt gatctgggac cgcgagttgg
     4441 aggcggcgtt gaaggagatg tacgaggcct ggtcctcctg gatccactgc ttctcggagg
     4501 tgatgccccg gtccaccagc cacccgacca gctccatgta gcgtgcggag gtttttgacc
     4561 ggatgacagg cgcgtcagaa ttggggttca gattctcctt gttctgctcc tgggtctggc
     4621 tgacgtgggt caggtgctgc gccacgagcc gtttgcgctc ggccaggttt aaacacgcgc
     4681 ttatatactc ctccatgtta gtccacgccc actgcagctc gggctgagtc ttgggcagga
     4741 ggtagttggg gatgtagcac tcgtccacca ccttgttccc ccctccggcg ccattacgcg
     4801 tcttggtcac cgcgaaccag ttgggcaggg tcggctcgat cccgcggtag atggtctgca
     4861 ccagcttgtc cctaatctga ctcaggaagc ggcccagcac catggatttg acccccgtgg
     4921 tctccaccag aatatggagg tggaagtagg actcgccctt ctcgaactga acaaagaaga
     4981 gggcctccgg ggccttactc acgcggcgcc actggaccag gaagtcgcgc tgcagcttct
     5041 cggccacggt caggggtgcc tgctcaatca gattcagatc catgtcagaa tctggcggca
     5101 actcccattc cttctcggcc acccagttca caaagctgtc agaaatgccg ggcagatgct
     5161 cgtcaaggtc gctggggacc ttaatcacaa tctcgtaaaa ccccggcatg gcggctgcgc
     5221 gttcaaacct cccgcttcaa aatggagacc ctgcgtgctc actcgggctt aaatacccag
     5281 cgtgaccaca tggtgtcgca aaatgtcgca aaacactcac gtgacctcta atacaagatc
     5341 ctctagagtc gacctgcagg catgcaagct tttgttccct ttagtgaggg ttaatttcga
     5401 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     5461 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     5521 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     5581 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     5641 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     5701 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     5761 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     5821 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     5881 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     5941 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     6001 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     6061 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     6121 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     6181 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     6241 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     6301 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     6361 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     6421 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     6481 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     6541 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     6601 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     6661 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     6721 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     6781 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     6841 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     6901 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     6961 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     7021 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     7081 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     7141 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     7201 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     7261 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     7321 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     7381 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     7441 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     7501 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcg
//
